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The map label for this gene is glnB [H]

Identifier: 213691145

GI number: 213691145

Start: 268614

End: 268952

Strand: Direct

Name: glnB [H]

Synonym: Blon_0224

Alternate gene names: 213691145

Gene position: 268614-268952 (Clockwise)

Preceding gene: 213691144

Following gene: 213691146

Centisome position: 9.48

GC content: 65.19

Gene sequence:

>339_bases
ATGAAGCTGATCACCGCTATCATTCAGCCTCATAAGCTCGACGATGTCAAGGAGGCCCTCGCGGCCGCCGGCGTGCACGG
TCTGACCGTGTCCGAGGCCAACGGCTACGGCCGCCAGCGCGGCCACACCGAGGTCTACCGTGGCGCTGAGTACACCGTGG
ACCTGATTCCGAAGATTCGCGTTGAAGTGCTGTCCGATGACGCGGATGCCGAGACGCTGGTCGGTGTGATCATCAAGTCC
GCCGGCACCGGCACCATCGGTGACGGCAAGGTGTGGGTCGCTCCGCTGGACTCCGTGGCCCGCGTGCGTACCGGTGAGAC
CGGTTCCGCCGCGATCTGA

Upstream 100 bases:

>100_bases
GAGGCCCAGGAAGTCGGCGGCATCGACCTTGCCGACCAGGGCGAACGTGCTTACGATTTTGCTGGTACTGCCAGCTCCGT
CCTCAAGGAGGTGAAGTGAA

Downstream 100 bases:

>100_bases
TAACACAGACGTCCATGCCTAGAAGGGGATGGACTGCATAGGAAAGGTCCCCGCTCGCCGCGAATTGACGCCGTGCGGGG
ACCTTTTTGGCAATGAAAGA

Product: nitrogen regulatory protein P-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MKLITAIIQPHKLDDVKEALAAAGVHGLTVSEANGYGRQRGHTEVYRGAEYTVDLIPKIRVEVLSDDADAETLVGVIIKS
AGTGTIGDGKVWVAPLDSVARVRTGETGSAAI

Sequences:

>Translated_112_residues
MKLITAIIQPHKLDDVKEALAAAGVHGLTVSEANGYGRQRGHTEVYRGAEYTVDLIPKIRVEVLSDDADAETLVGVIIKS
AGTGTIGDGKVWVAPLDSVARVRTGETGSAAI
>Mature_112_residues
MKLITAIIQPHKLDDVKEALAAAGVHGLTVSEANGYGRQRGHTEVYRGAEYTVDLIPKIRVEVLSDDADAETLVGVIIKS
AGTGTIGDGKVWVAPLDSVARVRTGETGSAAI

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=56.25, Blast_Score=134, Evalue=1e-33,
Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=51.7857142857143, Blast_Score=132, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 11723; Mature: 11723

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00496 PII_GLNB_UMP ; PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLITAIIQPHKLDDVKEALAAAGVHGLTVSEANGYGRQRGHTEVYRGAEYTVDLIPKIR
CCCEEECCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHCCCCCEEECCCCEE
VEVLSDDADAETLVGVIIKSAGTGTIGDGKVWVAPLDSVARVRTGETGSAAI
EEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHCCCCCCCCCC
>Mature Secondary Structure
MKLITAIIQPHKLDDVKEALAAAGVHGLTVSEANGYGRQRGHTEVYRGAEYTVDLIPKIR
CCCEEECCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHCCCCCEEECCCCEE
VEVLSDDADAETLVGVIIKSAGTGTIGDGKVWVAPLDSVARVRTGETGSAAI
EEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]