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The map label for this gene is amt [H]

Identifier: 213691144

GI number: 213691144

Start: 267317

End: 268612

Strand: Direct

Name: amt [H]

Synonym: Blon_0223

Alternate gene names: 213691144

Gene position: 267317-268612 (Clockwise)

Preceding gene: 213691143

Following gene: 213691145

Centisome position: 9.44

GC content: 61.88

Gene sequence:

>1296_bases
ATGGGAGCGATTGATTCCGGTAACACCGCATGGATTCTGACATCCGCGTCCTTGGTTTTCCTCATGACGCCTGGTGTGGC
GTTCTTCTACGGTGGTATGGTTCGCGCCAAGGCCGTACTGAACATGCTGATGCTTGAGGCGGCCGCGCTGTCGGTCACTA
TGATTATCTGGACTTTGTGGGGTTGGTCCATCGCATACGCCGGTTCCGACATCGGAGGCATCTTCGGCGACCCGGCCAGC
GGCTTCCTGCTGAAGGACTCCATGGTCGCGCAGGACGGCGTGTTCACCGCCACCGGTCTGAACGGCAACAACTACCCGGT
TTCTGTGGACGTCGCCTTCCAGGTCGCCTTCGCGATGATTACCGTAGGCCTGATCTGTGGCGCCATCGCCGAGCGTGTGA
AGTACAGCACCTGGATGATCTTCGTGGCGCTGTGGGTTACCTTCGACTACGCTCCTATGGCCCACATGGTCTGGAACGGT
GGTCTGCTGTCTGCTGACGGCGCCATCTCCAAGGCCATCGGCGCGGCCGCCCACGATTTCGCAGGTGGTACCGTGGTGCA
CATCAACGCCGCAGTCGCCGCCCTGATTATCGTGCTCATCATCGGCAAGCGTAAGGGCTTCGGCACTCAGCCGTTCCGCC
CGCACAACGTGCCGTTCGTGATGCTCGGCGCCTTCCTGCTGTGGTTCGGCTGGTTCGGCTTCAACGCTGGTTCCGCCTTC
GCCGCCAACGGTACCGCTGGTTATGCTTGGGTTTCCACTTCCGCCGCTACCGCCGCCGCAATGCTTTCGTGGGGCTTCAC
CGAGAAGATTCGCTCCGGCCACTACACCGCAATGGGTGCCGCTTCGGGTATGGTCGCCGGCCTGGTCGCCATCACCCCGG
CCGCCGATGTGGTTTCCCCGCTGTGGGCCATCGTGATGGGTGCCATCGCGGGTGTTCTGACCTGCCTGGCTTGCGGTCTC
AAGTTCAAGTTCGGTTACGATGACTCCCTCGACGTCGTCGGCGTACATGGCGTGGGTGGTTTCACCGGCACCATTCTCGT
TGGCTTCTTCGGCGAAGGCGCCGGTCTGCTGGCTGGTGGCGACTGGCGTCAGCTCGTTGTCCAGCTGGTCATCGCTCTCG
TCGCCATCCTCTACTCCGCGGTGATCACCGCGATCATCGCCTTCGCTCTGGAGAAGACCATTGGTTGGCGCGTCACCGAG
GCCCAGGAAGTCGGCGGCATCGACCTTGCCGACCAGGGCGAACGTGCTTACGATTTTGCTGGTACTGCCAGCTCCGTCCT
CAAGGAGGTGAAGTGA

Upstream 100 bases:

>100_bases
AAACTGTTAGCCCCCATAAGGGGGACGGTATTGTAGAGACAAATAAGACGCCGGCCGCACAAAGCCCCCGGTGCCATGAA
CGAAAGAGAGGGAGGTAGAC

Downstream 100 bases:

>100_bases
AATGAAGCTGATCACCGCTATCATTCAGCCTCATAAGCTCGACGATGTCAAGGAGGCCCTCGCGGCCGCCGGCGTGCACG
GTCTGACCGTGTCCGAGGCC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MGAIDSGNTAWILTSASLVFLMTPGVAFFYGGMVRAKAVLNMLMLEAAALSVTMIIWTLWGWSIAYAGSDIGGIFGDPAS
GFLLKDSMVAQDGVFTATGLNGNNYPVSVDVAFQVAFAMITVGLICGAIAERVKYSTWMIFVALWVTFDYAPMAHMVWNG
GLLSADGAISKAIGAAAHDFAGGTVVHINAAVAALIIVLIIGKRKGFGTQPFRPHNVPFVMLGAFLLWFGWFGFNAGSAF
AANGTAGYAWVSTSAATAAAMLSWGFTEKIRSGHYTAMGAASGMVAGLVAITPAADVVSPLWAIVMGAIAGVLTCLACGL
KFKFGYDDSLDVVGVHGVGGFTGTILVGFFGEGAGLLAGGDWRQLVVQLVIALVAILYSAVITAIIAFALEKTIGWRVTE
AQEVGGIDLADQGERAYDFAGTASSVLKEVK

Sequences:

>Translated_431_residues
MGAIDSGNTAWILTSASLVFLMTPGVAFFYGGMVRAKAVLNMLMLEAAALSVTMIIWTLWGWSIAYAGSDIGGIFGDPAS
GFLLKDSMVAQDGVFTATGLNGNNYPVSVDVAFQVAFAMITVGLICGAIAERVKYSTWMIFVALWVTFDYAPMAHMVWNG
GLLSADGAISKAIGAAAHDFAGGTVVHINAAVAALIIVLIIGKRKGFGTQPFRPHNVPFVMLGAFLLWFGWFGFNAGSAF
AANGTAGYAWVSTSAATAAAMLSWGFTEKIRSGHYTAMGAASGMVAGLVAITPAADVVSPLWAIVMGAIAGVLTCLACGL
KFKFGYDDSLDVVGVHGVGGFTGTILVGFFGEGAGLLAGGDWRQLVVQLVIALVAILYSAVITAIIAFALEKTIGWRVTE
AQEVGGIDLADQGERAYDFAGTASSVLKEVK
>Mature_430_residues
GAIDSGNTAWILTSASLVFLMTPGVAFFYGGMVRAKAVLNMLMLEAAALSVTMIIWTLWGWSIAYAGSDIGGIFGDPASG
FLLKDSMVAQDGVFTATGLNGNNYPVSVDVAFQVAFAMITVGLICGAIAERVKYSTWMIFVALWVTFDYAPMAHMVWNGG
LLSADGAISKAIGAAAHDFAGGTVVHINAAVAALIIVLIIGKRKGFGTQPFRPHNVPFVMLGAFLLWFGWFGFNAGSAFA
ANGTAGYAWVSTSAATAAAMLSWGFTEKIRSGHYTAMGAASGMVAGLVAITPAADVVSPLWAIVMGAIAGVLTCLACGLK
FKFGYDDSLDVVGVHGVGGFTGTILVGFFGEGAGLLAGGDWRQLVVQLVIALVAILYSAVITAIIAFALEKTIGWRVTEA
QEVGGIDLADQGERAYDFAGTASSVLKEVK

Specific function: Involved in the uptake of ammonia (Probable) [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=422, Percent_Identity=42.8909952606635, Blast_Score=288, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI17550180, Length=446, Percent_Identity=28.0269058295964, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17550182, Length=467, Percent_Identity=29.1220556745182, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32564042, Length=437, Percent_Identity=27.2311212814645, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI133931100, Length=441, Percent_Identity=25.1700680272109, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6325396, Length=430, Percent_Identity=31.8604651162791, Blast_Score=209, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6321559, Length=428, Percent_Identity=29.9065420560748, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6324187, Length=435, Percent_Identity=32.8735632183908, Blast_Score=194, Evalue=3e-50,
Organism=Drosophila melanogaster, GI161078314, Length=437, Percent_Identity=27.6887871853547, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 44755; Mature: 44623

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAIDSGNTAWILTSASLVFLMTPGVAFFYGGMVRAKAVLNMLMLEAAALSVTMIIWTLW
CCCCCCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GWSIAYAGSDIGGIFGDPASGFLLKDSMVAQDGVFTATGLNGNNYPVSVDVAFQVAFAMI
CCCEEECCCCCCCCCCCCCCCEEEECCHHHCCCCEEEECCCCCCCEEEEHHHHHHHHHHH
TVGLICGAIAERVKYSTWMIFVALWVTFDYAPMAHMVWNGGLLSADGAISKAIGAAAHDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEECCCHHHHHHHHHHHHC
AGGTVVHINAAVAALIIVLIIGKRKGFGTQPFRPHNVPFVMLGAFLLWFGWFGFNAGSAF
CCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEE
AANGTAGYAWVSTSAATAAAMLSWGFTEKIRSGHYTAMGAASGMVAGLVAITPAADVVSP
ECCCCCCEEEEECHHHHHHHHHHCCHHHHHCCCCCEECCCHHHHHHHHHHCCCHHHHHHH
LWAIVMGAIAGVLTCLACGLKFKFGYDDSLDVVGVHGVGGFTGTILVGFFGEGAGLLAGG
HHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEECCCCCCCCEEEEEEECCCCCEEECC
DWRQLVVQLVIALVAILYSAVITAIIAFALEKTIGWRVTEAQEVGGIDLADQGERAYDFA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECHHHCCCCCCCCCCCCHHCCC
GTASSVLKEVK
CCHHHHHHHCC
>Mature Secondary Structure 
GAIDSGNTAWILTSASLVFLMTPGVAFFYGGMVRAKAVLNMLMLEAAALSVTMIIWTLW
CCCCCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GWSIAYAGSDIGGIFGDPASGFLLKDSMVAQDGVFTATGLNGNNYPVSVDVAFQVAFAMI
CCCEEECCCCCCCCCCCCCCCEEEECCHHHCCCCEEEECCCCCCCEEEEHHHHHHHHHHH
TVGLICGAIAERVKYSTWMIFVALWVTFDYAPMAHMVWNGGLLSADGAISKAIGAAAHDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEECCCHHHHHHHHHHHHC
AGGTVVHINAAVAALIIVLIIGKRKGFGTQPFRPHNVPFVMLGAFLLWFGWFGFNAGSAF
CCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEE
AANGTAGYAWVSTSAATAAAMLSWGFTEKIRSGHYTAMGAASGMVAGLVAITPAADVVSP
ECCCCCCEEEEECHHHHHHHHHHCCHHHHHCCCCCEECCCHHHHHHHHHHCCCHHHHHHH
LWAIVMGAIAGVLTCLACGLKFKFGYDDSLDVVGVHGVGGFTGTILVGFFGEGAGLLAGG
HHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEECCCCCCCCEEEEEEECCCCCEEECC
DWRQLVVQLVIALVAILYSAVITAIIAFALEKTIGWRVTEAQEVGGIDLADQGERAYDFA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECHHHCCCCCCCCCCCCHHCCC
GTASSVLKEVK
CCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]