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The map label for this gene is ask [H]

Identifier: 213691085

GI number: 213691085

Start: 193268

End: 194032

Strand: Direct

Name: ask [H]

Synonym: Blon_0164

Alternate gene names: 213691085

Gene position: 193268-194032 (Clockwise)

Preceding gene: 213691084

Following gene: 213691086

Centisome position: 6.82

GC content: 63.4

Gene sequence:

>765_bases
GTGGCTCTCATCGTGCAGAAGTACGGCGGCTCGTCGGTCGCCGATACCGAATCGATCAAACGTGTGGCCAAGCGCGTTGT
TGAGACGGAAAAGAAGGGCAACAAGGTGGCCGTGGTGGTCTCCGCCATGGGCGACACCACCGATGACCTTATCGATCAGG
CGCTGAGCATCGACTCCAACCCGCCCGAGCGCGAGATGGACATGCTGATGACCGCAGGCGAGCGAATTTCCATGAGCCTG
CTGGCCGTGGCCATTCACGCCGAAGGCAGCCGCGCCCACTCCTTCACCGGTCAGCAGGCCGGCTTCTTCACCGACGCCCG
TTACGGCGCGGCCCACATCAAGGCCGTGAGACCGGACCGCGTGAAAAACGCGCTCTCATTGGGGGACATCGCCATCGTTG
CCGGCTTCCAGGGCATCAACGCCAAGGGTGACGCCACCACGCTCGGTCGCGGTGGTTCGGACACGTCCGCCGTGGCACTC
GCTGTGGCCCTTGGTGCCGACATCTGCGAGATTTACACCGACGTGGACGGCATCTTTACCGCTGATCCGCGCATCGTGCC
TTCTGCCCGCCGCATTCCGTCCATCGATTATGAGTCGATTCTGGAGATGGCCTCCTGCGGCTCCAAGGTGCTGGCACTGC
GCTGCGTAGAGTACGCTCAGCGTTTCAACATGCCGTTGCACGTGCGCTCTTCGTTCTCCCGTCGCCCCGGCACGCTGGTT
GTGCCTGACGGCATTGACCCGCGCACGCTGCCGAACCTCGACTGA

Upstream 100 bases:

>100_bases
GGCGCGGGGTCGCGGGGTGAACGCGGCAACCTTTATAATCGTTGCGTTTGCGTAAAATATCGACCAAACAAATCTCTAAG
AGAAGAACAAAAGGACAGCA

Downstream 100 bases:

>100_bases
GCGCGGCTACAGCGAACAATAATCGTAAGTAATTTCAACCAAGACAAGGCAAGACATGACTGAAGAAGAAGCGAAATCCA
TGGGCGACCTGTTCCCTGAC

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase; ASK [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MALIVQKYGGSSVADTESIKRVAKRVVETEKKGNKVAVVVSAMGDTTDDLIDQALSIDSNPPEREMDMLMTAGERISMSL
LAVAIHAEGSRAHSFTGQQAGFFTDARYGAAHIKAVRPDRVKNALSLGDIAIVAGFQGINAKGDATTLGRGGSDTSAVAL
AVALGADICEIYTDVDGIFTADPRIVPSARRIPSIDYESILEMASCGSKVLALRCVEYAQRFNMPLHVRSSFSRRPGTLV
VPDGIDPRTLPNLD

Sequences:

>Translated_254_residues
MALIVQKYGGSSVADTESIKRVAKRVVETEKKGNKVAVVVSAMGDTTDDLIDQALSIDSNPPEREMDMLMTAGERISMSL
LAVAIHAEGSRAHSFTGQQAGFFTDARYGAAHIKAVRPDRVKNALSLGDIAIVAGFQGINAKGDATTLGRGGSDTSAVAL
AVALGADICEIYTDVDGIFTADPRIVPSARRIPSIDYESILEMASCGSKVLALRCVEYAQRFNMPLHVRSSFSRRPGTLV
VPDGIDPRTLPNLD
>Mature_253_residues
ALIVQKYGGSSVADTESIKRVAKRVVETEKKGNKVAVVVSAMGDTTDDLIDQALSIDSNPPEREMDMLMTAGERISMSLL
AVAIHAEGSRAHSFTGQQAGFFTDARYGAAHIKAVRPDRVKNALSLGDIAIVAGFQGINAKGDATTLGRGGSDTSAVALA
VALGADICEIYTDVDGIFTADPRIVPSARRIPSIDYESILEMASCGSKVLALRCVEYAQRFNMPLHVRSSFSRRPGTLVV
PDGIDPRTLPNLD

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=112, Percent_Identity=43.75, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790455, Length=119, Percent_Identity=46.218487394958, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1790376, Length=285, Percent_Identity=28.0701754385965, Blast_Score=74, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6320893, Length=193, Percent_Identity=27.979274611399, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 27009; Mature: 26878

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIVQKYGGSSVADTESIKRVAKRVVETEKKGNKVAVVVSAMGDTTDDLIDQALSIDSN
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHCCCCC
PPEREMDMLMTAGERISMSLLAVAIHAEGSRAHSFTGQQAGFFTDARYGAAHIKAVRPDR
CCHHHHHHHHHCCCHHHHEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHEEECCCHH
VKNALSLGDIAIVAGFQGINAKGDATTLGRGGSDTSAVALAVALGADICEIYTDVDGIFT
HHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
ADPRIVPSARRIPSIDYESILEMASCGSKVLALRCVEYAQRFNMPLHVRSSFSRRPGTLV
CCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEE
VPDGIDPRTLPNLD
ECCCCCCCCCCCCC
>Mature Secondary Structure 
ALIVQKYGGSSVADTESIKRVAKRVVETEKKGNKVAVVVSAMGDTTDDLIDQALSIDSN
EEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHCCCCC
PPEREMDMLMTAGERISMSLLAVAIHAEGSRAHSFTGQQAGFFTDARYGAAHIKAVRPDR
CCHHHHHHHHHCCCHHHHEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHEEECCCHH
VKNALSLGDIAIVAGFQGINAKGDATTLGRGGSDTSAVALAVALGADICEIYTDVDGIFT
HHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
ADPRIVPSARRIPSIDYESILEMASCGSKVLALRCVEYAQRFNMPLHVRSSFSRRPGTLV
CCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEE
VPDGIDPRTLPNLD
ECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7910936 [H]