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The map label for this gene is grpE [H]

Identifier: 213691063

GI number: 213691063

Start: 171234

End: 171920

Strand: Direct

Name: grpE [H]

Synonym: Blon_0142

Alternate gene names: 213691063

Gene position: 171234-171920 (Clockwise)

Preceding gene: 213691062

Following gene: 213691064

Centisome position: 6.04

GC content: 63.17

Gene sequence:

>687_bases
ATGTCCGAGTTCAACAAGGACGACTACCTGAACGACCTGCCGGATCCCTCAGATGCTGAGGCTGCCGGCCAGGCCGCTCA
GGCCTCTTCAGGGGCTGACGCCTCCGCCGAGTCCGACTCTGCGCAGGATTCGGCTGCCCAGGCTCCTTCCAATGAAGGAG
CCGACGCGGCTCCTGCCGCAGCTGAGGGAGAGAAGTCGGATGCCGGAGAGAAGACTGGCGAAGGCCAGTCCGATTCCGAA
GACACCCTCACCCCCCTCAGCAAGGCCAAGAAGGAAGCCGCAGACTACCTCGAAGCCCTGCAACGCGAACGTGCGGAGTT
CATCAACTACCGCAATCGCACGCAGAAGGAGCAGGAGCGCTTCCGCCAGCACGGCATCATCGACGTGCTGACCGCGCTGC
TGCCCGCCCTCGACGATATCGACCGCATTCGCGAACACAGCGAGATGGACGACTCGTTCAAGGCCGTTGCCACCAAGATC
GACAAAGCCTTCGAGAAGTTCGGCGTCGAGAAGTTCGGCGAAAAGGGCGAGGACTTCGACCCCACCAAGCATGACGCGAT
CCTGCACAAGCCGGACGCGAATGCCGACAAGGAAACGGTTGACACCGTGGTTGAGGCCGGCTACCGCATCGGCGACCGCG
TAATCCGCGCCGCCCGTGTGGTCGTGGCCTCCCCGCAAGACCGATGA

Upstream 100 bases:

>100_bases
CTGCCGGCGCTGGCGCTTCCGCTGGCTCCGCCTCCGGTTCCGACGACGACACCGTCGAGGCCGAGGTCGTGGACGACGAT
GACGACAAGGACAACAAGTG

Downstream 100 bases:

>100_bases
CATGTTGGCTCCCCTCGATGAGGGGAGCCAACGCGAAGAACGTATACGAAAGGAGACATGAATGGCTGAAAACGAATGGC
TCAGCAAAGACTTCTACAAA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MSEFNKDDYLNDLPDPSDAEAAGQAAQASSGADASAESDSAQDSAAQAPSNEGADAAPAAAEGEKSDAGEKTGEGQSDSE
DTLTPLSKAKKEAADYLEALQRERAEFINYRNRTQKEQERFRQHGIIDVLTALLPALDDIDRIREHSEMDDSFKAVATKI
DKAFEKFGVEKFGEKGEDFDPTKHDAILHKPDANADKETVDTVVEAGYRIGDRVIRAARVVVASPQDR

Sequences:

>Translated_228_residues
MSEFNKDDYLNDLPDPSDAEAAGQAAQASSGADASAESDSAQDSAAQAPSNEGADAAPAAAEGEKSDAGEKTGEGQSDSE
DTLTPLSKAKKEAADYLEALQRERAEFINYRNRTQKEQERFRQHGIIDVLTALLPALDDIDRIREHSEMDDSFKAVATKI
DKAFEKFGVEKFGEKGEDFDPTKHDAILHKPDANADKETVDTVVEAGYRIGDRVIRAARVVVASPQDR
>Mature_227_residues
SEFNKDDYLNDLPDPSDAEAAGQAAQASSGADASAESDSAQDSAAQAPSNEGADAAPAAAEGEKSDAGEKTGEGQSDSED
TLTPLSKAKKEAADYLEALQRERAEFINYRNRTQKEQERFRQHGIIDVLTALLPALDDIDRIREHSEMDDSFKAVATKID
KAFEKFGVEKFGEKGEDFDPTKHDAILHKPDANADKETVDTVVEAGYRIGDRVIRAARVVVASPQDR

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324806, Length=172, Percent_Identity=27.3255813953488, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 24702; Mature: 24571

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFNKDDYLNDLPDPSDAEAAGQAAQASSGADASAESDSAQDSAAQAPSNEGADAAPAA
CCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCC
AEGEKSDAGEKTGEGQSDSEDTLTPLSKAKKEAADYLEALQRERAEFINYRNRTQKEQER
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
FRQHGIIDVLTALLPALDDIDRIREHSEMDDSFKAVATKIDKAFEKFGVEKFGEKGEDFD
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCC
PTKHDAILHKPDANADKETVDTVVEAGYRIGDRVIRAARVVVASPQDR
CCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SEFNKDDYLNDLPDPSDAEAAGQAAQASSGADASAESDSAQDSAAQAPSNEGADAAPAA
CCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCC
AEGEKSDAGEKTGEGQSDSEDTLTPLSKAKKEAADYLEALQRERAEFINYRNRTQKEQER
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
FRQHGIIDVLTALLPALDDIDRIREHSEMDDSFKAVATKIDKAFEKFGVEKFGEKGEDFD
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCC
PTKHDAILHKPDANADKETVDTVVEAGYRIGDRVIRAARVVVASPQDR
CCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]