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The map label for this gene is amiC [H]

Identifier: 213691025

GI number: 213691025

Start: 124700

End: 126001

Strand: Direct

Name: amiC [H]

Synonym: Blon_0104

Alternate gene names: 213691025

Gene position: 124700-126001 (Clockwise)

Preceding gene: 213691024

Following gene: 213691026

Centisome position: 4.4

GC content: 59.29

Gene sequence:

>1302_bases
ATGGCGAAGTTGGGAATGATGCGACCGGTCGTGGCCGCTATGGTGCTCGCCGCGGTGGTCGCCTTGACCAGTGGCTGTGG
CTGGGGCCGCACCGTGGCCGACAGAAAGGCCATGGAATCCGATGGCTCCGGCACTCCTGCGCAAAGCTGTGTGGATACCA
GCGCAAAGAGCATCGCCATCGGTTTCGTGAACTCGCTGAGCGGCACCATGGCCCTCTCTGAGAAGACCGTGTCCGATTCG
CTGCATATGGCGGTCGATGAGATCAACGCCAAGGGCGGTGTGCTCGGCAAGAAATTGGAAGTCGTGCAACAGGACGGCAA
GTCCGAACCGGCGGTGTTCGCGGAGAAAGCCAAGACCCTGATTGAAAAGAACTGCGTGGCGGCGGTGTTCGGCGGATGGA
CTTCCGCGTCCCGCAAAGCCATGAAGCCGGTGTTCGAAGCGGACGACGCGCTGCTGTTCTATCCGCTGCAGTATGAAGGC
ATGGAGGCCAGCCCCAATATCTTCTATACGGGTGCGGCGCCGAACCAGCAGATCGTTCCCGCTCTCGACTATCTCAAGGA
GCAAGGCCGCAGAAGGCTGTTCCTTGTGGGATCCGATTATGTGTTCCCCCAAACCGCGAACCGCATCGTGCGCGCCTATG
CGAAACACAATGGCATGCAGATCGTCGGAGAGGAATACACGCCGATGGGTGCCACGGACTTCAGCACCATCGTCAATAAG
CTCAAGGCCTCGGGGGCTGACGCCGTGGTCAACACGCTGAACGGTGATTCCAACGTCGCGTTCTTCAAGGAATACAAGGC
GTCGGGTCTGCAGGCGGACAGTCTTCCCGTCATCTCCATGTCCATCGCGGAAGAAGAAGTCGCCTCGATCGGCCCGGACA
ATGTCAAAGGCCAGTTGACCGCATGGAACTACTACCAGACTTTGGGCGACGAGGAAAACAGGACCTTCGTGAAGGATTTC
AAAGCCAGGTACGGGCATGACAGACCGACCTCGGACCCGATGGAATCCGCCTACACCTCCGTGTACCTGTGGAAGGAAAT
GGTCGAGAAGGCGAAGAGCTTCGCCGTGGATGACGTAAAGCGCGCCGCCGGCGGCATCACGTTCGATGCCCCCGAAGGCA
CGGTCACCGTCGATGGCGAAAACCACCACATCTACAAGACGTCGTATATCGGCCGGATCGGCGATGATCGCCTGATCCAT
ACCGTGTGGAAGTCCGATGGCCCCATCAAGCCCGATCCGTATCTCAAGGCGTATGCGTGGGCCGGGAGCCTGCAGAGTCC
GGATTTGGATTCATCCAAGTGA

Upstream 100 bases:

>100_bases
TGAGACGTTTTGGAAACACAAGGGCAACACGACGCGCGAAAGGGGTTGGGATAAATGGGCATGTAACTGCTTGGGCGTCG
TGCGAGAGAGGAACAGGCGT

Downstream 100 bases:

>100_bases
GTGAAGGGAGAAGGAGGGATGAACATGCTTGCACCGCGGATTGTCGAGGGACTGCCAAGCGGCTCGATATGGTCCCAGCA
GGCCCGATTCGTATCTCAAG

Product: Extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MAKLGMMRPVVAAMVLAAVVALTSGCGWGRTVADRKAMESDGSGTPAQSCVDTSAKSIAIGFVNSLSGTMALSEKTVSDS
LHMAVDEINAKGGVLGKKLEVVQQDGKSEPAVFAEKAKTLIEKNCVAAVFGGWTSASRKAMKPVFEADDALLFYPLQYEG
MEASPNIFYTGAAPNQQIVPALDYLKEQGRRRLFLVGSDYVFPQTANRIVRAYAKHNGMQIVGEEYTPMGATDFSTIVNK
LKASGADAVVNTLNGDSNVAFFKEYKASGLQADSLPVISMSIAEEEVASIGPDNVKGQLTAWNYYQTLGDEENRTFVKDF
KARYGHDRPTSDPMESAYTSVYLWKEMVEKAKSFAVDDVKRAAGGITFDAPEGTVTVDGENHHIYKTSYIGRIGDDRLIH
TVWKSDGPIKPDPYLKAYAWAGSLQSPDLDSSK

Sequences:

>Translated_433_residues
MAKLGMMRPVVAAMVLAAVVALTSGCGWGRTVADRKAMESDGSGTPAQSCVDTSAKSIAIGFVNSLSGTMALSEKTVSDS
LHMAVDEINAKGGVLGKKLEVVQQDGKSEPAVFAEKAKTLIEKNCVAAVFGGWTSASRKAMKPVFEADDALLFYPLQYEG
MEASPNIFYTGAAPNQQIVPALDYLKEQGRRRLFLVGSDYVFPQTANRIVRAYAKHNGMQIVGEEYTPMGATDFSTIVNK
LKASGADAVVNTLNGDSNVAFFKEYKASGLQADSLPVISMSIAEEEVASIGPDNVKGQLTAWNYYQTLGDEENRTFVKDF
KARYGHDRPTSDPMESAYTSVYLWKEMVEKAKSFAVDDVKRAAGGITFDAPEGTVTVDGENHHIYKTSYIGRIGDDRLIH
TVWKSDGPIKPDPYLKAYAWAGSLQSPDLDSSK
>Mature_432_residues
AKLGMMRPVVAAMVLAAVVALTSGCGWGRTVADRKAMESDGSGTPAQSCVDTSAKSIAIGFVNSLSGTMALSEKTVSDSL
HMAVDEINAKGGVLGKKLEVVQQDGKSEPAVFAEKAKTLIEKNCVAAVFGGWTSASRKAMKPVFEADDALLFYPLQYEGM
EASPNIFYTGAAPNQQIVPALDYLKEQGRRRLFLVGSDYVFPQTANRIVRAYAKHNGMQIVGEEYTPMGATDFSTIVNKL
KASGADAVVNTLNGDSNVAFFKEYKASGLQADSLPVISMSIAEEEVASIGPDNVKGQLTAWNYYQTLGDEENRTFVKDFK
ARYGHDRPTSDPMESAYTSVYLWKEMVEKAKSFAVDDVKRAAGGITFDAPEGTVTVDGENHHIYKTSYIGRIGDDRLIHT
VWKSDGPIKPDPYLKAYAWAGSLQSPDLDSSK

Specific function: Negatively regulates the expression of the aliphatic amidase operon. AmiC functions by inhibiting the action of AmiR at the protein level. It exhibits protein kinase activity [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 46816; Mature: 46685

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLGMMRPVVAAMVLAAVVALTSGCGWGRTVADRKAMESDGSGTPAQSCVDTSAKSIAI
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHCCHHEEEE
GFVNSLSGTMALSEKTVSDSLHMAVDEINAKGGVLGKKLEVVQQDGKSEPAVFAEKAKTL
EEHHHCCCCCEECHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHH
IEKNCVAAVFGGWTSASRKAMKPVFEADDALLFYPLQYEGMEASPNIFYTGAAPNQQIVP
HHHCCCEEEECCCCHHHHHHHCHHHCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCHH
ALDYLKEQGRRRLFLVGSDYVFPQTANRIVRAYAKHNGMQIVGEEYTPMGATDFSTIVNK
HHHHHHHCCCEEEEEEECCEECCHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHH
LKASGADAVVNTLNGDSNVAFFKEYKASGLQADSLPVISMSIAEEEVASIGPDNVKGQLT
HHCCCCCHHHEECCCCCCEEHHHHHHHCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCEEE
AWNYYQTLGDEENRTFVKDFKARYGHDRPTSDPMESAYTSVYLWKEMVEKAKSFAVDDVK
EEHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAAGGITFDAPEGTVTVDGENHHIYKTSYIGRIGDDRLIHTVWKSDGPIKPDPYLKAYAW
HHHCCCEEECCCCEEEECCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCEEEEEE
AGSLQSPDLDSSK
CCCCCCCCCCCCC
>Mature Secondary Structure 
AKLGMMRPVVAAMVLAAVVALTSGCGWGRTVADRKAMESDGSGTPAQSCVDTSAKSIAI
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHCCHHEEEE
GFVNSLSGTMALSEKTVSDSLHMAVDEINAKGGVLGKKLEVVQQDGKSEPAVFAEKAKTL
EEHHHCCCCCEECHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHH
IEKNCVAAVFGGWTSASRKAMKPVFEADDALLFYPLQYEGMEASPNIFYTGAAPNQQIVP
HHHCCCEEEECCCCHHHHHHHCHHHCCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCHH
ALDYLKEQGRRRLFLVGSDYVFPQTANRIVRAYAKHNGMQIVGEEYTPMGATDFSTIVNK
HHHHHHHCCCEEEEEEECCEECCHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHH
LKASGADAVVNTLNGDSNVAFFKEYKASGLQADSLPVISMSIAEEEVASIGPDNVKGQLT
HHCCCCCHHHEECCCCCCEEHHHHHHHCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCEEE
AWNYYQTLGDEENRTFVKDFKARYGHDRPTSDPMESAYTSVYLWKEMVEKAKSFAVDDVK
EEHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAAGGITFDAPEGTVTVDGENHHIYKTSYIGRIGDDRLIHTVWKSDGPIKPDPYLKAYAW
HHHCCCEEECCCCEEEECCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCEEEEEE
AGSLQSPDLDSSK
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1907262; 10984043; 1762155; 7813419; 10508151; 10708652 [H]