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The map label for this gene is dnaN [H]

Identifier: 213690931

GI number: 213690931

Start: 2447

End: 3571

Strand: Direct

Name: dnaN [H]

Synonym: Blon_0003

Alternate gene names: 213690931

Gene position: 2447-3571 (Clockwise)

Preceding gene: 213690930

Following gene: 213690932

Centisome position: 0.09

GC content: 60.71

Gene sequence:

>1125_bases
ATGAAAGTCGAAATCGATACCGCAGCCCTGGCCGACGCCGTAGCCTGGACCTCCCGAGTCATCGACGCACGTCCCTCCAA
CCCGATTCTCGCTGGCGTCAAGCTGGAAGCCATTGAAGGAACGCTGCAATTCTCCGCCTTCAACTACGAGATTTCCGCTC
GCCACCACATCGAAGCCGGCGTTGACGAAGCCGGTTCCGTACTGGTTCAGGGCAAACTGCTGGCCGATATCACCAAATCG
CTGCGTTCGGAGAAAACGTACCTGGTTACCGACGGCTCCACGCTGACCATCACGTCCGGCAAGTCGAAGTTCACCTTGCA
GCTCATGCCCGACACCGAGTACCCGGATCTGCCTGTGGTACCGGCCATGCTCGGCCAAGTCGATGCCCCGACTTTCGTTC
AGGCAGTGAACCAAGCATGCGTTGCCGTCTCCCGCGAAGAGAACCGCCCGGTGCTGACCGGCGTACGCATGCAGTTCCAA
GGCGACAAGGTCGTCATGACCTCCACCGATCGCTTCCGTTTGGCCCGCGCCACCTTCACTTGGACTCCGCAGAACCCGGA
CGTCGACACCACCACGCTGGTTCGCGGCTCACTACTCAAGGATGTGGCCCGCGCTCTTGATGAGCACCAGAACATCCGCC
TTGATTTCGACGCCGATAGCCCGACGCTGCTCGGCTTCGAAAACGCCGGCCGCGTCTCCACCTCCCAGCTCATCGACGGT
GAATTCCCGGCGGTCGACCGCCTGTTCGCCGACGAATACCCGATTCAGGCCGTAGTCAACAAGCAGGATCTGCTCGACGC
CATCAGCCGTGTGGCTTTGGTCGCCGAACGCAATGCTCCGATTCGCATGACCTTCACCGGTCAGGAAGTGGCGCTGTCCG
CCGGATCGGTCGACGAGGCCCAGGCCAACGAGACACTGGACATCGACATGGATGGCGACGACATCACCGTGGCATTCAAC
CCGTCCTACCTGAAGGAGGGTCTGTCCGCCGTGACCGAACCATTCGTGCGCATCAAGATGACGACCCCGGTCAAGCCGGT
CGAGTTCAACGGCCAGCAGGAAGCTGATTCCGACGAGTCCATGGACTACCGATACCTGCTCGTGCCGATGCGCTTTAACA
ACTGA

Upstream 100 bases:

>100_bases
CATCATCGCCACAGTAACAGCCGGTCACCCATCGTACGTAAGTTCGCCACCCAACGGCTAGAATATTTCTCAGCCAATCC
GACGAAGGGAACAATCACAC

Downstream 100 bases:

>100_bases
AGTCCCTGAATTTCCAACGATTCGATAATGCACATCTCCCGTCTCGCACTCGACCACTACCGCTCATGGAGTCAGGTAGT
GGTCGATTTCGTACCAGGCG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKVEIDTAALADAVAWTSRVIDARPSNPILAGVKLEAIEGTLQFSAFNYEISARHHIEAGVDEAGSVLVQGKLLADITKS
LRSEKTYLVTDGSTLTITSGKSKFTLQLMPDTEYPDLPVVPAMLGQVDAPTFVQAVNQACVAVSREENRPVLTGVRMQFQ
GDKVVMTSTDRFRLARATFTWTPQNPDVDTTTLVRGSLLKDVARALDEHQNIRLDFDADSPTLLGFENAGRVSTSQLIDG
EFPAVDRLFADEYPIQAVVNKQDLLDAISRVALVAERNAPIRMTFTGQEVALSAGSVDEAQANETLDIDMDGDDITVAFN
PSYLKEGLSAVTEPFVRIKMTTPVKPVEFNGQQEADSDESMDYRYLLVPMRFNN

Sequences:

>Translated_374_residues
MKVEIDTAALADAVAWTSRVIDARPSNPILAGVKLEAIEGTLQFSAFNYEISARHHIEAGVDEAGSVLVQGKLLADITKS
LRSEKTYLVTDGSTLTITSGKSKFTLQLMPDTEYPDLPVVPAMLGQVDAPTFVQAVNQACVAVSREENRPVLTGVRMQFQ
GDKVVMTSTDRFRLARATFTWTPQNPDVDTTTLVRGSLLKDVARALDEHQNIRLDFDADSPTLLGFENAGRVSTSQLIDG
EFPAVDRLFADEYPIQAVVNKQDLLDAISRVALVAERNAPIRMTFTGQEVALSAGSVDEAQANETLDIDMDGDDITVAFN
PSYLKEGLSAVTEPFVRIKMTTPVKPVEFNGQQEADSDESMDYRYLLVPMRFNN
>Mature_374_residues
MKVEIDTAALADAVAWTSRVIDARPSNPILAGVKLEAIEGTLQFSAFNYEISARHHIEAGVDEAGSVLVQGKLLADITKS
LRSEKTYLVTDGSTLTITSGKSKFTLQLMPDTEYPDLPVVPAMLGQVDAPTFVQAVNQACVAVSREENRPVLTGVRMQFQ
GDKVVMTSTDRFRLARATFTWTPQNPDVDTTTLVRGSLLKDVARALDEHQNIRLDFDADSPTLLGFENAGRVSTSQLIDG
EFPAVDRLFADEYPIQAVVNKQDLLDAISRVALVAERNAPIRMTFTGQEVALSAGSVDEAQANETLDIDMDGDDITVAFN
PSYLKEGLSAVTEPFVRIKMTTPVKPVEFNGQQEADSDESMDYRYLLVPMRFNN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=381, Percent_Identity=23.0971128608924, Blast_Score=95, Evalue=8e-21,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40982; Mature: 40982

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVEIDTAALADAVAWTSRVIDARPSNPILAGVKLEAIEGTLQFSAFNYEISARHHIEAG
CEEEECHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECEEEEEEEEEEEECCEEECCC
VDEAGSVLVQGKLLADITKSLRSEKTYLVTDGSTLTITSGKSKFTLQLMPDTEYPDLPVV
CCCCCCEEEECHHHHHHHHHHHCCCEEEEECCCEEEEECCCCEEEEEEECCCCCCCCCCH
PAMLGQVDAPTFVQAVNQACVAVSREENRPVLTGVRMQFQGDKVVMTSTDRFRLARATFT
HHHHCCCCCCHHHHHHHHHHEEEECCCCCCEEEEEEEEECCCEEEEECCCCEEEEEEEEE
WTPQNPDVDTTTLVRGSLLKDVARALDEHQNIRLDFDADSPTLLGFENAGRVSTSQLIDG
ECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCHHHCCCC
EFPAVDRLFADEYPIQAVVNKQDLLDAISRVALVAERNAPIRMTFTGQEVALSAGSVDEA
CCCHHHHHHCCCCCHHEECCHHHHHHHHHHHEEEEECCCCEEEEECCCCEEEECCCCCCC
QANETLDIDMDGDDITVAFNPSYLKEGLSAVTEPFVRIKMTTPVKPVEFNGQQEADSDES
CCCCEEEEEECCCEEEEEECHHHHHHHHHHHCCCEEEEEEECCCCCEECCCCCCCCCCCC
MDYRYLLVPMRFNN
CCEEEEEEEEEECC
>Mature Secondary Structure
MKVEIDTAALADAVAWTSRVIDARPSNPILAGVKLEAIEGTLQFSAFNYEISARHHIEAG
CEEEECHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECEEEEEEEEEEEECCEEECCC
VDEAGSVLVQGKLLADITKSLRSEKTYLVTDGSTLTITSGKSKFTLQLMPDTEYPDLPVV
CCCCCCEEEECHHHHHHHHHHHCCCEEEEECCCEEEEECCCCEEEEEEECCCCCCCCCCH
PAMLGQVDAPTFVQAVNQACVAVSREENRPVLTGVRMQFQGDKVVMTSTDRFRLARATFT
HHHHCCCCCCHHHHHHHHHHEEEECCCCCCEEEEEEEEECCCEEEEECCCCEEEEEEEEE
WTPQNPDVDTTTLVRGSLLKDVARALDEHQNIRLDFDADSPTLLGFENAGRVSTSQLIDG
ECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCCCCCHHHCCCC
EFPAVDRLFADEYPIQAVVNKQDLLDAISRVALVAERNAPIRMTFTGQEVALSAGSVDEA
CCCHHHHHHCCCCCHHEECCHHHHHHHHHHHEEEEECCCCEEEEECCCCEEEECCCCCCC
QANETLDIDMDGDDITVAFNPSYLKEGLSAVTEPFVRIKMTTPVKPVEFNGQQEADSDES
CCCCEEEEEECCCEEEEEECHHHHHHHHHHHCCCEEEEEEECCCCCEECCCCCCCCCCCC
MDYRYLLVPMRFNN
CCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1577691; 12000953 [H]