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The map label for this gene is recF

Identifier: 213690932

GI number: 213690932

Start: 3599

End: 4837

Strand: Direct

Name: recF

Synonym: Blon_0004

Alternate gene names: 213690932

Gene position: 3599-4837 (Clockwise)

Preceding gene: 213690931

Following gene: 213690933

Centisome position: 0.13

GC content: 57.47

Gene sequence:

>1239_bases
ATGCACATCTCCCGTCTCGCACTCGACCACTACCGCTCATGGAGTCAGGTAGTGGTCGATTTCGTACCAGGCGTGAATAT
CCTGTTCGGCAAGAATGGCTTGGGCAAAACCAATCTGGTGGAAGCCGTCGAAGTGCTGTCCACCGGATCGAGCCATCGCA
CGTCCAGCACGTTGCCGCTGATCGAACGCGGGCAGACCACGGCCACCATCCGCGCGAACGTCGCTGATGATGCCGGCCAA
ACCACTACGTATGAAGCGTCGATTCATGCGCGTGGTGCCAACCGAGCCCGCATCAACTCGGGGTCGTCGCTGTATCTGCG
CGATATCATCGGCAAGATTCCCAGCGTTTCCTTCACTCCCGAAGATCAGCGATTGGTGTCCGGCGATCCGGGAGCACGCC
GCACCATGATGAATCAGGCTGCGGCTCTGCTCGAGCCCGGATACATGCAGACGTTGCAACAGTTCACGCGCATCGCCAAA
CAGCGTGCCACATTGTTGAAGCAATTGAATGCCAATGCCAATAATGGCCAGCCGATGGATGCGGTTCTGAGCGGTTTGGA
AATCTGGACCGGCCAGTTCATCGAAGCCGGTGTGGTGTTGACCCGTATGCGAGCCCATGTGATTAGCCTGCTTGCCGAGC
CATTTGCCGCGATTTACGCCGACTTGGCCGGTGCCGGCGAGCAGGTGACGCTCACATATGCGCCTTCATTCGACGAAGTG
CTGATGTTCAACGATCCGCATCCGCAGATCAGCGAGCATTTCCAACGCATCTACCCCGGTGAAGTGGCTCGCGGCGTGAA
CCTTATCGGTCCGCAGCGCGATGACATGAACCTTGACTTGGCCGGTATTCCCGCCCGCGAGTTCGCTTCGAACGGCGAGA
TGTGGACGATGGCGTTGGCCTTGAAAATGGCATTGTTCGAAATCGTGCGTGACCGGTTGGGATTGCAGCCGATTGTGATT
CTCGATGATGTGTTCGCCCAGCTTGATGACAGCCGGCGCGCGCAGATTCTTGATTTCGCCAGAAAACAGGATCAGGTGCT
GATTACCGTGGCCGCTGAAGGCGATGTGCCCGACTATGAATCGGCCCATCGCATCGACGTGGCCGCACTGGCCGAACCAG
CTTCCGTGATACTGCCAACTCTGGGCAATTTGTCCGATTTGCGGGAAAACGAGAGTTTGGGTAAGTATGACGGAAAAACC
GCGGTGGACAGCGCGTCCGTAGAACGGAAAGTGTCATGA

Upstream 100 bases:

>100_bases
CCAGCAGGAAGCTGATTCCGACGAGTCCATGGACTACCGATACCTGCTCGTGCCGATGCGCTTTAACAACTGAAGTCCCT
GAATTTCCAACGATTCGATA

Downstream 100 bases:

>100_bases
ACCCGCCGATCGCCCGCCAACTCAACCTGGACGAGACCAAACTGCCGGCCGAAATATTCGAGCGGATCTCCCGTCGCGGG
GCGACCTTGAAGGACTACAG

Product: DNA replication and repair protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MHISRLALDHYRSWSQVVVDFVPGVNILFGKNGLGKTNLVEAVEVLSTGSSHRTSSTLPLIERGQTTATIRANVADDAGQ
TTTYEASIHARGANRARINSGSSLYLRDIIGKIPSVSFTPEDQRLVSGDPGARRTMMNQAAALLEPGYMQTLQQFTRIAK
QRATLLKQLNANANNGQPMDAVLSGLEIWTGQFIEAGVVLTRMRAHVISLLAEPFAAIYADLAGAGEQVTLTYAPSFDEV
LMFNDPHPQISEHFQRIYPGEVARGVNLIGPQRDDMNLDLAGIPAREFASNGEMWTMALALKMALFEIVRDRLGLQPIVI
LDDVFAQLDDSRRAQILDFARKQDQVLITVAAEGDVPDYESAHRIDVAALAEPASVILPTLGNLSDLRENESLGKYDGKT
AVDSASVERKVS

Sequences:

>Translated_412_residues
MHISRLALDHYRSWSQVVVDFVPGVNILFGKNGLGKTNLVEAVEVLSTGSSHRTSSTLPLIERGQTTATIRANVADDAGQ
TTTYEASIHARGANRARINSGSSLYLRDIIGKIPSVSFTPEDQRLVSGDPGARRTMMNQAAALLEPGYMQTLQQFTRIAK
QRATLLKQLNANANNGQPMDAVLSGLEIWTGQFIEAGVVLTRMRAHVISLLAEPFAAIYADLAGAGEQVTLTYAPSFDEV
LMFNDPHPQISEHFQRIYPGEVARGVNLIGPQRDDMNLDLAGIPAREFASNGEMWTMALALKMALFEIVRDRLGLQPIVI
LDDVFAQLDDSRRAQILDFARKQDQVLITVAAEGDVPDYESAHRIDVAALAEPASVILPTLGNLSDLRENESLGKYDGKT
AVDSASVERKVS
>Mature_412_residues
MHISRLALDHYRSWSQVVVDFVPGVNILFGKNGLGKTNLVEAVEVLSTGSSHRTSSTLPLIERGQTTATIRANVADDAGQ
TTTYEASIHARGANRARINSGSSLYLRDIIGKIPSVSFTPEDQRLVSGDPGARRTMMNQAAALLEPGYMQTLQQFTRIAK
QRATLLKQLNANANNGQPMDAVLSGLEIWTGQFIEAGVVLTRMRAHVISLLAEPFAAIYADLAGAGEQVTLTYAPSFDEV
LMFNDPHPQISEHFQRIYPGEVARGVNLIGPQRDDMNLDLAGIPAREFASNGEMWTMALALKMALFEIVRDRLGLQPIVI
LDDVFAQLDDSRRAQILDFARKQDQVLITVAAEGDVPDYESAHRIDVAALAEPASVILPTLGNLSDLRENESLGKYDGKT
AVDSASVERKVS

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=362, Percent_Identity=23.2044198895028, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_BIFLI (B7GSG2)

Other databases:

- EMBL:   CP001095
- RefSeq:   YP_002321518.1
- GeneID:   7054721
- GenomeReviews:   CP001095_GR
- KEGG:   bln:Blon_0004
- HOGENOM:   HBG645124
- ProtClustDB:   CLSK572721
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 44909; Mature: 44909

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHISRLALDHYRSWSQVVVDFVPGVNILFGKNGLGKTNLVEAVEVLSTGSSHRTSSTLPL
CCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCH
IERGQTTATIRANVADDAGQTTTYEASIHARGANRARINSGSSLYLRDIIGKIPSVSFTP
HHCCCCEEEEEEECCCCCCCCEEEEEEEEECCCCCCEECCCCCCHHHHHHHHCCCCCCCC
EDQRLVSGDPGARRTMMNQAAALLEPGYMQTLQQFTRIAKQRATLLKQLNANANNGQPMD
CCCEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
AVLSGLEIWTGQFIEAGVVLTRMRAHVISLLAEPFAAIYADLAGAGEQVTLTYAPSFDEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCE
LMFNDPHPQISEHFQRIYPGEVARGVNLIGPQRDDMNLDLAGIPAREFASNGEMWTMALA
EEECCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCEEEECCCHHHHHCCCCCHHHHHH
LKMALFEIVRDRLGLQPIVILDDVFAQLDDSRRAQILDFARKQDQVLITVAAEGDVPDYE
HHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCC
SAHRIDVAALAEPASVILPTLGNLSDLRENESLGKYDGKTAVDSASVERKVS
CCCCEEHHHHCCCHHHHHHCCCCHHHHHCCCCCCCCCCCCHHCCCCHHHCCC
>Mature Secondary Structure
MHISRLALDHYRSWSQVVVDFVPGVNILFGKNGLGKTNLVEAVEVLSTGSSHRTSSTLPL
CCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCH
IERGQTTATIRANVADDAGQTTTYEASIHARGANRARINSGSSLYLRDIIGKIPSVSFTP
HHCCCCEEEEEEECCCCCCCCEEEEEEEEECCCCCCEECCCCCCHHHHHHHHCCCCCCCC
EDQRLVSGDPGARRTMMNQAAALLEPGYMQTLQQFTRIAKQRATLLKQLNANANNGQPMD
CCCEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
AVLSGLEIWTGQFIEAGVVLTRMRAHVISLLAEPFAAIYADLAGAGEQVTLTYAPSFDEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCE
LMFNDPHPQISEHFQRIYPGEVARGVNLIGPQRDDMNLDLAGIPAREFASNGEMWTMALA
EEECCCCHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCEEEECCCHHHHHCCCCCHHHHHH
LKMALFEIVRDRLGLQPIVILDDVFAQLDDSRRAQILDFARKQDQVLITVAAEGDVPDYE
HHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCC
SAHRIDVAALAEPASVILPTLGNLSDLRENESLGKYDGKTAVDSASVERKVS
CCCCEEHHHHCCCHHHHHHCCCCHHHHHCCCCCCCCCCCCHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA