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The map label for this gene is pucK [H]

Identifier: 213159094

GI number: 213159094

Start: 3922568

End: 3923935

Strand: Direct

Name: pucK [H]

Synonym: AB57_3804

Alternate gene names: 213159094

Gene position: 3922568-3923935 (Clockwise)

Preceding gene: 213159093

Following gene: 213159095

Centisome position: 96.84

GC content: 42.84

Gene sequence:

>1368_bases
ATGAATCATCAGCAAAAGAATGCGATTTCTCCAGAGCATGAATATTTAGGAATTTCCAAAAGTTTTGCTTACGGCTTACA
GCATGTGCTCACCATGTATGGGGGCATTGTAGCCCCGCCACTGATTATTGGAACGGCAGCTGGCTTAAGCGCAGCACAAG
TCGGAATGCTCATTGCAGCGGCCCTATTTGTAGGCGGGTTGGCAACTCTCATACAAACCATCGGAACCAAATATCTAGGG
GCAAAATTACCTCTAGTGCAAGGTGTGTCTTTTGCTGGTGTGGCAACCATGGTTGCTATTATCACTACAGGCGGTGGTTT
ACCTGCTGTCTACGGCGCGGTTATTGCCGCATCTTTAATTGGACTATGCCTTGCGCCTTACTTTTCAAAAATTATTCGTT
TTTTTCCACCTGTTGTGACGGGATGTGTCATCACGATTATTGGGTTATCTCTGTTACCCGTTGCGATTCGCTGGATGATG
GGTGGTAACAACAAAGCACCTGATTGGGGTAGCGTTGAGAATATCTCTTTAGCTTTGCTGACCTTAGGCATTGTGATTAT
TCTAAATATACTGCCACAAGCAAGTATACGCCGTTTATCAATTTTGTTGGCTATTGTTGCTGGTACTACCCTTGCCTACT
TTATGGGTTTTGGAGATTTTAGTCAGGTGAGCTCAGGCGCATGGCTGCAATTTCCACACTTATTTGCATTTGGTTTACCA
ACCTTCGAGCTAAGTGCAATTTTATCGATGCTAATCGTTACTTTGGTGATTATGACGGAAACCACAGCCGATATTATTGC
TGTCGGAGATATTGTAGGAACCCAAGTCGATTCAAAACGTATCGCCAATGGTGTACGTGCAGATATGTTTTCAAGCGCTA
TTGCTCCTATCTTTGGTTCATTCATGCAAAGTGCTTTTGCCCAAAATGTTGGACTGGTGGCAATTACAGGCATCAAAAGC
CGCTTTGTCGTGGCAGCAGGCGGAGTCATTCTGATTATTTTAGGTCTATTACCTGTTATGGGCCGCCTTATTGCGGCAAT
TCCTATGCCAGTACTTGGTGGTGCGGGTTTGGTCTTATTTGGGTCGGTTGCAGCGAGTGGTATTCGCACTTTGGCAAAAA
TTGATTATAACGATCAGAAAAATTTGATTATTGTGGCAACTGCTTTATCAGCTGGGATGATTCCAATTATTAACCATGAA
TTTTATGCTCATTTCCCAGTTTGGGTACAAACTTTGTTCCATTCGGGTATTAGTTCGACTTGTATCTTTGCTATCTTATT
GAATTTGTTATTTAATCACTTACCTTCATTTCGCAGCTCGCGTACCCCTCATTTAAGCCAAACAATAAACACACGAAATA
CACATTAA

Upstream 100 bases:

>100_bases
AGAAGACACCAATCAGCATTACCACATTCCATTACTGATCAGCCCGTTTGCTTATTCCACTTATCGCGGCAGCTAACTCA
AATATAAATAGGAGAAAAAT

Downstream 100 bases:

>100_bases
TGAGTTAGTTTTTTAGCGCCCCACTTCGGTGGGCTTTTTTTTAACCGTTGATCAAATTTGGCTTCATTTTTGCTTAAGAC
CCTGTATCCTTAGCGTGCTT

Product: uracil-xanthine permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MNHQQKNAISPEHEYLGISKSFAYGLQHVLTMYGGIVAPPLIIGTAAGLSAAQVGMLIAAALFVGGLATLIQTIGTKYLG
AKLPLVQGVSFAGVATMVAIITTGGGLPAVYGAVIAASLIGLCLAPYFSKIIRFFPPVVTGCVITIIGLSLLPVAIRWMM
GGNNKAPDWGSVENISLALLTLGIVIILNILPQASIRRLSILLAIVAGTTLAYFMGFGDFSQVSSGAWLQFPHLFAFGLP
TFELSAILSMLIVTLVIMTETTADIIAVGDIVGTQVDSKRIANGVRADMFSSAIAPIFGSFMQSAFAQNVGLVAITGIKS
RFVVAAGGVILIILGLLPVMGRLIAAIPMPVLGGAGLVLFGSVAASGIRTLAKIDYNDQKNLIIVATALSAGMIPIINHE
FYAHFPVWVQTLFHSGISSTCIFAILLNLLFNHLPSFRSSRTPHLSQTINTRNTH

Sequences:

>Translated_455_residues
MNHQQKNAISPEHEYLGISKSFAYGLQHVLTMYGGIVAPPLIIGTAAGLSAAQVGMLIAAALFVGGLATLIQTIGTKYLG
AKLPLVQGVSFAGVATMVAIITTGGGLPAVYGAVIAASLIGLCLAPYFSKIIRFFPPVVTGCVITIIGLSLLPVAIRWMM
GGNNKAPDWGSVENISLALLTLGIVIILNILPQASIRRLSILLAIVAGTTLAYFMGFGDFSQVSSGAWLQFPHLFAFGLP
TFELSAILSMLIVTLVIMTETTADIIAVGDIVGTQVDSKRIANGVRADMFSSAIAPIFGSFMQSAFAQNVGLVAITGIKS
RFVVAAGGVILIILGLLPVMGRLIAAIPMPVLGGAGLVLFGSVAASGIRTLAKIDYNDQKNLIIVATALSAGMIPIINHE
FYAHFPVWVQTLFHSGISSTCIFAILLNLLFNHLPSFRSSRTPHLSQTINTRNTH
>Mature_455_residues
MNHQQKNAISPEHEYLGISKSFAYGLQHVLTMYGGIVAPPLIIGTAAGLSAAQVGMLIAAALFVGGLATLIQTIGTKYLG
AKLPLVQGVSFAGVATMVAIITTGGGLPAVYGAVIAASLIGLCLAPYFSKIIRFFPPVVTGCVITIIGLSLLPVAIRWMM
GGNNKAPDWGSVENISLALLTLGIVIILNILPQASIRRLSILLAIVAGTTLAYFMGFGDFSQVSSGAWLQFPHLFAFGLP
TFELSAILSMLIVTLVIMTETTADIIAVGDIVGTQVDSKRIANGVRADMFSSAIAPIFGSFMQSAFAQNVGLVAITGIKS
RFVVAAGGVILIILGLLPVMGRLIAAIPMPVLGGAGLVLFGSVAASGIRTLAKIDYNDQKNLIIVATALSAGMIPIINHE
FYAHFPVWVQTLFHSGISSTCIFAILLNLLFNHLPSFRSSRTPHLSQTINTRNTH

Specific function: Uptake of uric acid [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680148, Length=440, Percent_Identity=24.0909090909091, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI40316845, Length=440, Percent_Identity=24.0909090909091, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI44680145, Length=432, Percent_Identity=24.7685185185185, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI44680143, Length=436, Percent_Identity=24.5412844036697, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI87082181, Length=431, Percent_Identity=38.9791183294664, Blast_Score=291, Evalue=5e-80,
Organism=Escherichia coli, GI1790087, Length=421, Percent_Identity=33.2541567695962, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI87082178, Length=467, Percent_Identity=28.4796573875803, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1788843, Length=388, Percent_Identity=27.8350515463918, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI87081818, Length=456, Percent_Identity=28.2894736842105, Blast_Score=123, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17558856, Length=187, Percent_Identity=26.2032085561497, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21356175, Length=436, Percent_Identity=24.3119266055046, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017588
- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 47769; Mature: 47769

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHQQKNAISPEHEYLGISKSFAYGLQHVLTMYGGIVAPPLIIGTAAGLSAAQVGMLIAA
CCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCHHHCCHHHHHHHCCCHHHHHHHHHHH
ALFVGGLATLIQTIGTKYLGAKLPLVQGVSFAGVATMVAIITTGGGLPAVYGAVIAASLI
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GLCLAPYFSKIIRFFPPVVTGCVITIIGLSLLPVAIRWMMGGNNKAPDWGSVENISLALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
TLGIVIILNILPQASIRRLSILLAIVAGTTLAYFMGFGDFSQVSSGAWLQFPHLFAFGLP
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEECHHHHHHCCC
TFELSAILSMLIVTLVIMTETTADIIAVGDIVGTQVDSKRIANGVRADMFSSAIAPIFGS
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FMQSAFAQNVGLVAITGIKSRFVVAAGGVILIILGLLPVMGRLIAAIPMPVLGGAGLVLF
HHHHHHHCCCCEEEEECCCCCHHEECCHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHH
GSVAASGIRTLAKIDYNDQKNLIIVATALSAGMIPIINHEFYAHFPVWVQTLFHSGISST
HHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHCCCHHH
CIFAILLNLLFNHLPSFRSSRTPHLSQTINTRNTH
HHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MNHQQKNAISPEHEYLGISKSFAYGLQHVLTMYGGIVAPPLIIGTAAGLSAAQVGMLIAA
CCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCHHHCCHHHHHHHCCCHHHHHHHHHHH
ALFVGGLATLIQTIGTKYLGAKLPLVQGVSFAGVATMVAIITTGGGLPAVYGAVIAASLI
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GLCLAPYFSKIIRFFPPVVTGCVITIIGLSLLPVAIRWMMGGNNKAPDWGSVENISLALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
TLGIVIILNILPQASIRRLSILLAIVAGTTLAYFMGFGDFSQVSSGAWLQFPHLFAFGLP
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEECHHHHHHCCC
TFELSAILSMLIVTLVIMTETTADIIAVGDIVGTQVDSKRIANGVRADMFSSAIAPIFGS
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FMQSAFAQNVGLVAITGIKSRFVVAAGGVILIILGLLPVMGRLIAAIPMPVLGGAGLVLF
HHHHHHHCCCCEEEEECCCCCHHEECCHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHH
GSVAASGIRTLAKIDYNDQKNLIIVATALSAGMIPIINHEFYAHFPVWVQTLFHSGISST
HHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHCCCHHH
CIFAILLNLLFNHLPSFRSSRTPHLSQTINTRNTH
HHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]