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The map label for this gene is ddl

Identifier: 213159075

GI number: 213159075

Start: 3903962

End: 3904888

Strand: Reverse

Name: ddl

Synonym: AB57_3785

Alternate gene names: 213159075

Gene position: 3904888-3903962 (Counterclockwise)

Preceding gene: 213159076

Following gene: 213159074

Centisome position: 96.4

GC content: 43.69

Gene sequence:

>927_bases
GTGTCAAATGCTACAAAATTCGGCAAAGTTGCCGTGTTACTTGGTGGGAAATCGGCTGAGCGTGCTGTGTCTTTAGATAG
TGGTCAGGCAGTTTTGGACGCCTTGTTACGTTCAGGTGTTCAGGCTGAGGCATTTGATCCGCAAAACCGTAGCGTAACTG
AACTTGTAAACTATGACCGTGCATTTATTGTATTGCATGGTCGTGGTGGTGAAGACGGTCAAATCCAAGGTGTGCTTGAG
TGGTTGAACATCCCTTATACAGGAACGGGTGTACAAGGTTCTGCCATTGGTATGGATAAAGTCAAAACCAAGCAAATCTG
GCAAGGTAGTGATTTACCAACCGCACCGTATCGCATTATTACTAAAGAAACTGACTTGGATTCAGTGATTGCTGAATTGG
GGTTGCCTGTCATTATCAAGCCTGTACATGAAGGTTCAAGTGTAGGCATGAGTAAGGTTGAGAAAGCTGAAGATTTTGCG
GCAGCAATTGAAAAGGCAACTCAGCACGATGCTGTAGTTATGGCAGAAAAGTGGATTACAGGACGCGAATTTACGATTTC
ATTCTTAAATGGTCAGCCTCTACCTGTTATTCGTTTGCAGCCACCAGCAGATGTTGCTTTCTATGACTACGAAGCAAAAT
ATCAACGTAATGATGTTGAGTATGGCATTCCTTGTGGTCTTAGCGAAACTGAAGAGAAAAAACTACAAGCTCTATGTTTA
CGCGCTTTTCAAGCCGTAGGTGCAGAAGGCTGGGGACGTATTGATGCAATGCAGGATGAGCAAGGTAACTTCTGGCTTTT
AGAAGTAAATACTGTGCCAGGCATGACCAGTCATTCGCTTGTACCAAAGGCTGCTAAAGCAGTAGGTTATAGCTTTGATG
AATTGTGTGTTGCAATTCTTGATCAAACATTGGAAGGTACGGCTTAA

Upstream 100 bases:

>100_bases
GTGCAGGTAACGTGGGAGCAATTTCAGTAGAACTTGCACAGCACCATTTGTATGTGAAATAAAACAAAAAGTATTGAATT
ATCGGGTTAAGGATTTAAAC

Downstream 100 bases:

>100_bases
ATATGGCACAACTTCCGGCATCCATGCGCCGTAAACGTGCGGCAATTACCTCCATTCATGAAAAACCGCCTACGCGCAAG
CAAAAACTTGCCAATGCCGG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSNATKFGKVAVLLGGKSAERAVSLDSGQAVLDALLRSGVQAEAFDPQNRSVTELVNYDRAFIVLHGRGGEDGQIQGVLE
WLNIPYTGTGVQGSAIGMDKVKTKQIWQGSDLPTAPYRIITKETDLDSVIAELGLPVIIKPVHEGSSVGMSKVEKAEDFA
AAIEKATQHDAVVMAEKWITGREFTISFLNGQPLPVIRLQPPADVAFYDYEAKYQRNDVEYGIPCGLSETEEKKLQALCL
RAFQAVGAEGWGRIDAMQDEQGNFWLLEVNTVPGMTSHSLVPKAAKAVGYSFDELCVAILDQTLEGTA

Sequences:

>Translated_308_residues
MSNATKFGKVAVLLGGKSAERAVSLDSGQAVLDALLRSGVQAEAFDPQNRSVTELVNYDRAFIVLHGRGGEDGQIQGVLE
WLNIPYTGTGVQGSAIGMDKVKTKQIWQGSDLPTAPYRIITKETDLDSVIAELGLPVIIKPVHEGSSVGMSKVEKAEDFA
AAIEKATQHDAVVMAEKWITGREFTISFLNGQPLPVIRLQPPADVAFYDYEAKYQRNDVEYGIPCGLSETEEKKLQALCL
RAFQAVGAEGWGRIDAMQDEQGNFWLLEVNTVPGMTSHSLVPKAAKAVGYSFDELCVAILDQTLEGTA
>Mature_307_residues
SNATKFGKVAVLLGGKSAERAVSLDSGQAVLDALLRSGVQAEAFDPQNRSVTELVNYDRAFIVLHGRGGEDGQIQGVLEW
LNIPYTGTGVQGSAIGMDKVKTKQIWQGSDLPTAPYRIITKETDLDSVIAELGLPVIIKPVHEGSSVGMSKVEKAEDFAA
AIEKATQHDAVVMAEKWITGREFTISFLNGQPLPVIRLQPPADVAFYDYEAKYQRNDVEYGIPCGLSETEEKKLQALCLR
AFQAVGAEGWGRIDAMQDEQGNFWLLEVNTVPGMTSHSLVPKAAKAVGYSFDELCVAILDQTLEGTA

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=302, Percent_Identity=52.6490066225166, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1786579, Length=351, Percent_Identity=29.9145299145299, Blast_Score=153, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_ACIB3 (B7GV75)

Other databases:

- EMBL:   CP001172
- RefSeq:   YP_002324088.1
- ProteinModelPortal:   B7GV75
- SMR:   B7GV75
- GeneID:   7058878
- GenomeReviews:   CP001172_GR
- KEGG:   abb:ABBFA_000147
- HOGENOM:   HBG427092
- OMA:   EVLVEKW
- ProtClustDB:   PRK01372
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33346; Mature: 33215

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNATKFGKVAVLLGGKSAERAVSLDSGQAVLDALLRSGVQAEAFDPQNRSVTELVNYDR
CCCCCCCCEEEEEECCCCCCCEEECCCCHHHHHHHHHCCCCEECCCCCCCCHHHHHCCCE
AFIVLHGRGGEDGQIQGVLEWLNIPYTGTGVQGSAIGMDKVKTKQIWQGSDLPTAPYRII
EEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEE
TKETDLDSVIAELGLPVIIKPVHEGSSVGMSKVEKAEDFAAAIEKATQHDAVVMAEKWIT
EECCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEHHCCC
GREFTISFLNGQPLPVIRLQPPADVAFYDYEAKYQRNDVEYGIPCGLSETEEKKLQALCL
CCEEEEEEECCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RAFQAVGAEGWGRIDAMQDEQGNFWLLEVNTVPGMTSHSLVPKAAKAVGYSFDELCVAIL
HHHHHHCCCCCCCCCCEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCHHHHHHHHH
DQTLEGTA
HHHHCCCC
>Mature Secondary Structure 
SNATKFGKVAVLLGGKSAERAVSLDSGQAVLDALLRSGVQAEAFDPQNRSVTELVNYDR
CCCCCCCEEEEEECCCCCCCEEECCCCHHHHHHHHHCCCCEECCCCCCCCHHHHHCCCE
AFIVLHGRGGEDGQIQGVLEWLNIPYTGTGVQGSAIGMDKVKTKQIWQGSDLPTAPYRII
EEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEE
TKETDLDSVIAELGLPVIIKPVHEGSSVGMSKVEKAEDFAAAIEKATQHDAVVMAEKWIT
EECCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEHHCCC
GREFTISFLNGQPLPVIRLQPPADVAFYDYEAKYQRNDVEYGIPCGLSETEEKKLQALCL
CCEEEEEEECCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RAFQAVGAEGWGRIDAMQDEQGNFWLLEVNTVPGMTSHSLVPKAAKAVGYSFDELCVAIL
HHHHHHCCCCCCCCCCEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCHHHHHHHHH
DQTLEGTA
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA