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The map label for this gene is nemA [H]

Identifier: 213159053

GI number: 213159053

Start: 3878850

End: 3879908

Strand: Reverse

Name: nemA [H]

Synonym: AB57_3762

Alternate gene names: 213159053

Gene position: 3879908-3878850 (Counterclockwise)

Preceding gene: 213159054

Following gene: 213159052

Centisome position: 95.79

GC content: 42.3

Gene sequence:

>1059_bases
ATGGCTGAACTCAATACTCCCTTAACAGTTGGTGATTTTGAAATTAAAAATCGACTGGTAATGGCCCCATTAACACGTGC
GCGTAGTGGCGAAAGCCGTGTTCCAAACGATTTAATGGTGGAATATTATCAGCAACGTGCAAATGCTGGTCTCATTCTCA
CTGAAGCAACAGTGATTGGTTCAAAAACCGTAGGTTATGCAGATACTCCGGGACTATGGTCACAAGAGCAAGCACAAGCA
TGGAATAAGATTATTGAAGCAGTTCATGCTCAAGGTTCAAAAATTGTAGTTCAACTGTGGCATGTGGGTCGTATTTCACA
TCCAGAGCTTTTAAATGGCGATATTCCTGTTGCACCGAGTGCGATTCAACCTGCGGGTGAAGTAAGTTTATTACGCCCTA
AACGTCCATATGTAACGCCACGTGCGCTTTCAATTGAAGAAATTCAGGAAATTGTTGCTCAATATAAGCATTCAGCTGAA
TTGGCTAAAGCAGCAGGTTTTGATGGCGTTGAGCTACATGCAGCAAATGGTTATTTAATTGACCAGTTCCTTCAAAGTAA
TACTAACCAGCGTGATGATGAGTATGGTGGTCCGGTTGAAAACCGAGCTCGTTTGTTATTAGAAGTGGTTGATGCCTTTA
TTGAAGTGTGGGGAGCTGGCCGAGTAGGTGTGCATATTGCACCGCGTGGCGACTCGCATGATATGGGTGATGAAAACCCG
CTAGCGACATTTGGTTATGTGGTTGACCAACTTAGCCAACGTAATGTGGCTTTCATTTTCTCTCGTGAATATGAAGCAGC
TGATAGTATTAGCCCACAATTACGTGAAAAATTTAATGGTGTATGGATTGCCAATGAAAATCTGACACCAGAGTCAGCAA
AACGTATCTTGCGTGAAGGTCAAGCAGATGCTGTATCTTTTGGTAAAGCATATATCGCTAACCCAGATCTATTACAGCGT
TTAGAACAAGGCTTACCATTAAATGAACTTCAACCAACGACTTTATATGCTAAAGGCGCTGAAGGATATACAGACTATCC
GGCTTTAGAAGCTTCATAA

Upstream 100 bases:

>100_bases
CAATGGTCTTATTTTTCACGTAACGAAGCTTTGATTCAGCAATATATCGAACATTTAAAACAAACACTTTAAAACAAATA
AAGTGTAAGTCGAGGCGATC

Downstream 100 bases:

>100_bases
GTTCATAATATTATTTAAATTTACTATATGCTGAACTTGCTCTTTTGTGAGTTCAGTAAATGACTTTTCTTGTATATGGT
AGAGTTTAGGTATCGAAACT

Product: N-ethylmaleimide reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MAELNTPLTVGDFEIKNRLVMAPLTRARSGESRVPNDLMVEYYQQRANAGLILTEATVIGSKTVGYADTPGLWSQEQAQA
WNKIIEAVHAQGSKIVVQLWHVGRISHPELLNGDIPVAPSAIQPAGEVSLLRPKRPYVTPRALSIEEIQEIVAQYKHSAE
LAKAAGFDGVELHAANGYLIDQFLQSNTNQRDDEYGGPVENRARLLLEVVDAFIEVWGAGRVGVHIAPRGDSHDMGDENP
LATFGYVVDQLSQRNVAFIFSREYEAADSISPQLREKFNGVWIANENLTPESAKRILREGQADAVSFGKAYIANPDLLQR
LEQGLPLNELQPTTLYAKGAEGYTDYPALEAS

Sequences:

>Translated_352_residues
MAELNTPLTVGDFEIKNRLVMAPLTRARSGESRVPNDLMVEYYQQRANAGLILTEATVIGSKTVGYADTPGLWSQEQAQA
WNKIIEAVHAQGSKIVVQLWHVGRISHPELLNGDIPVAPSAIQPAGEVSLLRPKRPYVTPRALSIEEIQEIVAQYKHSAE
LAKAAGFDGVELHAANGYLIDQFLQSNTNQRDDEYGGPVENRARLLLEVVDAFIEVWGAGRVGVHIAPRGDSHDMGDENP
LATFGYVVDQLSQRNVAFIFSREYEAADSISPQLREKFNGVWIANENLTPESAKRILREGQADAVSFGKAYIANPDLLQR
LEQGLPLNELQPTTLYAKGAEGYTDYPALEAS
>Mature_351_residues
AELNTPLTVGDFEIKNRLVMAPLTRARSGESRVPNDLMVEYYQQRANAGLILTEATVIGSKTVGYADTPGLWSQEQAQAW
NKIIEAVHAQGSKIVVQLWHVGRISHPELLNGDIPVAPSAIQPAGEVSLLRPKRPYVTPRALSIEEIQEIVAQYKHSAEL
AKAAGFDGVELHAANGYLIDQFLQSNTNQRDDEYGGPVENRARLLLEVVDAFIEVWGAGRVGVHIAPRGDSHDMGDENPL
ATFGYVVDQLSQRNVAFIFSREYEAADSISPQLREKFNGVWIANENLTPESAKRILREGQADAVSFGKAYIANPDLLQRL
EQGLPLNELQPTTLYAKGAEGYTDYPALEAS

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=362, Percent_Identity=46.1325966850829, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI1789463, Length=347, Percent_Identity=30.5475504322767, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17559804, Length=237, Percent_Identity=32.0675105485232, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI72001454, Length=345, Percent_Identity=25.5072463768116, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17564188, Length=237, Percent_Identity=29.957805907173, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17565138, Length=357, Percent_Identity=26.3305322128852, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17559802, Length=347, Percent_Identity=25.0720461095101, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17540738, Length=139, Percent_Identity=34.5323741007194, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17566914, Length=215, Percent_Identity=29.3023255813954, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325086, Length=375, Percent_Identity=36.8, Blast_Score=219, Evalue=7e-58,
Organism=Saccharomyces cerevisiae, GI6321973, Length=378, Percent_Identity=35.978835978836, Blast_Score=218, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38701; Mature: 38570

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELNTPLTVGDFEIKNRLVMAPLTRARSGESRVPNDLMVEYYQQRANAGLILTEATVIG
CCCCCCCEEECCHHHCCEEEECCHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEC
SKTVGYADTPGLWSQEQAQAWNKIIEAVHAQGSKIVVQLWHVGRISHPELLNGDIPVAPS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHCCCCCCCCCC
AIQPAGEVSLLRPKRPYVTPRALSIEEIQEIVAQYKHSAELAKAAGFDGVELHAANGYLI
CCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHH
DQFLQSNTNQRDDEYGGPVENRARLLLEVVDAFIEVWGAGRVGVHIAPRGDSHDMGDENP
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
LATFGYVVDQLSQRNVAFIFSREYEAADSISPQLREKFNGVWIANENLTPESAKRILREG
HHHHHHHHHHHCCCCEEEEEECCCCCHHCCCHHHHHHHCCEEEECCCCCHHHHHHHHHCC
QADAVSFGKAYIANPDLLQRLEQGLPLNELQPTTLYAKGAEGYTDYPALEAS
CCHHHHHCCHHCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
AELNTPLTVGDFEIKNRLVMAPLTRARSGESRVPNDLMVEYYQQRANAGLILTEATVIG
CCCCCCEEECCHHHCCEEEECCHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEEC
SKTVGYADTPGLWSQEQAQAWNKIIEAVHAQGSKIVVQLWHVGRISHPELLNGDIPVAPS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHCCCCCCCCCC
AIQPAGEVSLLRPKRPYVTPRALSIEEIQEIVAQYKHSAELAKAAGFDGVELHAANGYLI
CCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHH
DQFLQSNTNQRDDEYGGPVENRARLLLEVVDAFIEVWGAGRVGVHIAPRGDSHDMGDENP
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
LATFGYVVDQLSQRNVAFIFSREYEAADSISPQLREKFNGVWIANENLTPESAKRILREG
HHHHHHHHHHHCCCCEEEEEECCCCCHHCCCHHHHHHHCCEEEECCCCCHHHHHHHHHCC
QADAVSFGKAYIANPDLLQRLEQGLPLNELQPTTLYAKGAEGYTDYPALEAS
CCHHHHHCCHHCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]