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The map label for this gene is betT [H]

Identifier: 213159037

GI number: 213159037

Start: 3858739

End: 3860706

Strand: Reverse

Name: betT [H]

Synonym: AB57_3745

Alternate gene names: 213159037

Gene position: 3860706-3858739 (Counterclockwise)

Preceding gene: 213159038

Following gene: 213159035

Centisome position: 95.31

GC content: 40.75

Gene sequence:

>1968_bases
ATGCCTTCCAAACAAGCGAGTTGGTTCGCAAATGTTAATCCCAATGTCTTTTTGGGTACGGTCATTATTATTGCTTTGTT
CTTAGCAGTTGTTGTGATCGCTCCGAGTTCTTTTGAACTTCTCACGCAACAGTTAAAGCAGTGGATCACTGATTCTTTTA
GTTGGTTCTATGTCTTATCGGTTGCCTTCTTTCTTATCCTACTCATTTATATTGCCTGTTCTGCGATTGGACGTATTAAA
TTAGGCCCTGACCATAGCCAGCCCGAATATAGTAACGGATCATGGTTTGCCATGTTGTTTACTGCGGGTATGGGTATTGG
CTTAATGTTCTTTGGTGTGGCTGAACCTGTCATGCACTATGTCAACCCACCAAGTGGTGAGCCACAGACAATTGAGGCAG
CACAACAAGCTTTACGTGTAACCTTTTTCCACTGGGGCTTACATGCATGGGCAATTTACGCCGTGGTCGGTTTAGCATTA
GCTTATTTTGCTTATCGGCATAATTTACCGCTAAAAACCCGTTCAGCACTGTATCCACTGATTGGTAAAAAGATTTATGG
ACCGTGGGGCGACAGCATCGATATATTTGCGACCATTGGTACGGTATTTGGTGTAGCGACTTCTCTCGGATTTGGTGTTA
CCCAGATTAACTCTGGTTTACATTATCTATTTGGTATTGAGCAAAGCACCACTACTCAGGTTTTACTCATTATTTTTGTG
AGTATTCTCGCTTCATTATCTGTGTTCTTAGGACTAGATAAAGGGGTCAAACGTTTATCAGAGCTAAACTTAGTATTAGC
TTTAATATTATTAGTATTCGTTTTTATCGCTGGCCCTAGTATTTATTTACTTCAAACCACTATTCAAAACACTGGGCAGT
ATATCTCTAACCTTTTTACCATGACATTCAATTTATATGCTTATCAGCCGAATGGATGGATTGGGGGATGGACGATTTTA
TATTGGGCTTGGTGGATTTCTTGGTCGCCATTTGTAGGTATGTTTATCGCAAGGGTTTCACGTGGTCGTACTATTCGAGA
ATTTATTGTCGGGGTACTCCTCATTCCAACAGGCTTTACCATTATCTGGATGGGCTTTTTAGGAAATGCTGCGTTATTTA
GCATTATCCATGAACATCAAACCACTTTGATTCAGGCTGTTCAGCAAGACTCGTCAGTTGCTCTATTTGAGTTTTTAGGA
CACTTGCCATGGTCAGGTGTCATGAATATTCTGGCGACTATTTTGGTGGTTTTATTCTTTGTGACTTCTGCTGACTCAGG
GGCATTGGTTACTGACTACTTAACAGCAAAAACAGAAAATTCACCTACTTGGCAGCGCCTGTTCTGGACAGTGCTTATGG
CGGTGTTGGCTATTATTTTACTCTTGGTGGGCGGTCTTGCGGCGCTTCAGTCTTCAATCATTATGAGTGCGTTGCCTTTT
ACCGTAGTCATGCTGCTAATGAGTTGGGGATTAATAAAAGCCTTGCATCTTGATGTCACTAAAATGATAGCAATTCAAGA
AGCTCGCATTACACCACGCGCCATTCATAACCCTAGAAGCTGGCAGCAGCGCCTTGGTTTAATCATGCATTATCCTCATA
GTGAAGAAGAGGTGAAGCAATATATTGAAAAGCAGGTTTCACGTGCTTTTGAAAATATTCAGCATGAGTTTAGACGCCGT
CATCTTGAGGTGTCGATTAGTGAAGTGGAAGATGGTATGCAGCTTCGAGTCGATCATCATCATGAAATTAATTTTATTTA
TAAAGTTGTTTCACGTGAAACAGTACCTCCAAGTTTTTTAATTGGTCGTACAGAAGCAGAAGACGGTCAATATTTTCAAG
CTGAGGTATTTTTGAGAGAAGGCGGACAAAACTATGATGTAATGGACTGGACTCAGGAAGATTTGATTCAGGATATTATT
GATCAGTACGAACGTCATTTACATTTCTTAAATATCGTACGCAGTTAA

Upstream 100 bases:

>100_bases
ATTATCGGCTTTCTCTATTGAAAGATATAACTTTGTTCATTCCTAAATGTCTTTATTGGCTTTAAGCCTGTCATTTTCAT
TAAAATAACATGGAGGATTT

Downstream 100 bases:

>100_bases
TAGCCATAAAAAAAGGACTCAATTGAGTCCTTTTTTTTAATTCAATTTCTAAATTAAATTAGAAACGGAATTTAGCATTT
AAGCCGATAGTTTGTGTATC

Product: high-affinity choline transport protein

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 655; Mature: 654

Protein sequence:

>655_residues
MPSKQASWFANVNPNVFLGTVIIIALFLAVVVIAPSSFELLTQQLKQWITDSFSWFYVLSVAFFLILLIYIACSAIGRIK
LGPDHSQPEYSNGSWFAMLFTAGMGIGLMFFGVAEPVMHYVNPPSGEPQTIEAAQQALRVTFFHWGLHAWAIYAVVGLAL
AYFAYRHNLPLKTRSALYPLIGKKIYGPWGDSIDIFATIGTVFGVATSLGFGVTQINSGLHYLFGIEQSTTTQVLLIIFV
SILASLSVFLGLDKGVKRLSELNLVLALILLVFVFIAGPSIYLLQTTIQNTGQYISNLFTMTFNLYAYQPNGWIGGWTIL
YWAWWISWSPFVGMFIARVSRGRTIREFIVGVLLIPTGFTIIWMGFLGNAALFSIIHEHQTTLIQAVQQDSSVALFEFLG
HLPWSGVMNILATILVVLFFVTSADSGALVTDYLTAKTENSPTWQRLFWTVLMAVLAIILLLVGGLAALQSSIIMSALPF
TVVMLLMSWGLIKALHLDVTKMIAIQEARITPRAIHNPRSWQQRLGLIMHYPHSEEEVKQYIEKQVSRAFENIQHEFRRR
HLEVSISEVEDGMQLRVDHHHEINFIYKVVSRETVPPSFLIGRTEAEDGQYFQAEVFLREGGQNYDVMDWTQEDLIQDII
DQYERHLHFLNIVRS

Sequences:

>Translated_655_residues
MPSKQASWFANVNPNVFLGTVIIIALFLAVVVIAPSSFELLTQQLKQWITDSFSWFYVLSVAFFLILLIYIACSAIGRIK
LGPDHSQPEYSNGSWFAMLFTAGMGIGLMFFGVAEPVMHYVNPPSGEPQTIEAAQQALRVTFFHWGLHAWAIYAVVGLAL
AYFAYRHNLPLKTRSALYPLIGKKIYGPWGDSIDIFATIGTVFGVATSLGFGVTQINSGLHYLFGIEQSTTTQVLLIIFV
SILASLSVFLGLDKGVKRLSELNLVLALILLVFVFIAGPSIYLLQTTIQNTGQYISNLFTMTFNLYAYQPNGWIGGWTIL
YWAWWISWSPFVGMFIARVSRGRTIREFIVGVLLIPTGFTIIWMGFLGNAALFSIIHEHQTTLIQAVQQDSSVALFEFLG
HLPWSGVMNILATILVVLFFVTSADSGALVTDYLTAKTENSPTWQRLFWTVLMAVLAIILLLVGGLAALQSSIIMSALPF
TVVMLLMSWGLIKALHLDVTKMIAIQEARITPRAIHNPRSWQQRLGLIMHYPHSEEEVKQYIEKQVSRAFENIQHEFRRR
HLEVSISEVEDGMQLRVDHHHEINFIYKVVSRETVPPSFLIGRTEAEDGQYFQAEVFLREGGQNYDVMDWTQEDLIQDII
DQYERHLHFLNIVRS
>Mature_654_residues
PSKQASWFANVNPNVFLGTVIIIALFLAVVVIAPSSFELLTQQLKQWITDSFSWFYVLSVAFFLILLIYIACSAIGRIKL
GPDHSQPEYSNGSWFAMLFTAGMGIGLMFFGVAEPVMHYVNPPSGEPQTIEAAQQALRVTFFHWGLHAWAIYAVVGLALA
YFAYRHNLPLKTRSALYPLIGKKIYGPWGDSIDIFATIGTVFGVATSLGFGVTQINSGLHYLFGIEQSTTTQVLLIIFVS
ILASLSVFLGLDKGVKRLSELNLVLALILLVFVFIAGPSIYLLQTTIQNTGQYISNLFTMTFNLYAYQPNGWIGGWTILY
WAWWISWSPFVGMFIARVSRGRTIREFIVGVLLIPTGFTIIWMGFLGNAALFSIIHEHQTTLIQAVQQDSSVALFEFLGH
LPWSGVMNILATILVVLFFVTSADSGALVTDYLTAKTENSPTWQRLFWTVLMAVLAIILLLVGGLAALQSSIIMSALPFT
VVMLLMSWGLIKALHLDVTKMIAIQEARITPRAIHNPRSWQQRLGLIMHYPHSEEEVKQYIEKQVSRAFENIQHEFRRRH
LEVSISEVEDGMQLRVDHHHEINFIYKVVSRETVPPSFLIGRTEAEDGQYFQAEVFLREGGQNYDVMDWTQEDLIQDIID
QYERHLHFLNIVRS

Specific function: High-affinity uptake of choline driven by a proton- motive force [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=657, Percent_Identity=41.7047184170472, Blast_Score=531, Evalue=1e-152,
Organism=Escherichia coli, GI1788102, Length=459, Percent_Identity=30.5010893246187, Blast_Score=198, Evalue=9e-52,
Organism=Escherichia coli, GI1786224, Length=491, Percent_Identity=27.4949083503055, Blast_Score=190, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 73906; Mature: 73775

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSKQASWFANVNPNVFLGTVIIIALFLAVVVIAPSSFELLTQQLKQWITDSFSWFYVLS
CCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHH
VAFFLILLIYIACSAIGRIKLGPDHSQPEYSNGSWFAMLFTAGMGIGLMFFGVAEPVMHY
HHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
VNPPSGEPQTIEAAQQALRVTFFHWGLHAWAIYAVVGLALAYFAYRHNLPLKTRSALYPL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
IGKKIYGPWGDSIDIFATIGTVFGVATSLGFGVTQINSGLHYLFGIEQSTTTQVLLIIFV
HCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEECCCCHHHHHHHHHHH
SILASLSVFLGLDKGVKRLSELNLVLALILLVFVFIAGPSIYLLQTTIQNTGQYISNLFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHH
MTFNLYAYQPNGWIGGWTILYWAWWISWSPFVGMFIARVSRGRTIREFIVGVLLIPTGFT
HEEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHH
IIWMGFLGNAALFSIIHEHQTTLIQAVQQDSSVALFEFLGHLPWSGVMNILATILVVLFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VTSADSGALVTDYLTAKTENSPTWQRLFWTVLMAVLAIILLLVGGLAALQSSIIMSALPF
HHCCCCCCEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVVMLLMSWGLIKALHLDVTKMIAIQEARITPRAIHNPRSWQQRLGLIMHYPHSEEEVKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHCCEEEECCCCHHHHHH
YIEKQVSRAFENIQHEFRRRHLEVSISEVEDGMQLRVDHHHEINFIYKVVSRETVPPSFL
HHHHHHHHHHHHHHHHHHHHHHHEEHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCCE
IGRTEAEDGQYFQAEVFLREGGQNYDVMDWTQEDLIQDIIDQYERHLHFLNIVRS
EECCCCCCCCEEEEEHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PSKQASWFANVNPNVFLGTVIIIALFLAVVVIAPSSFELLTQQLKQWITDSFSWFYVLS
CCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHH
VAFFLILLIYIACSAIGRIKLGPDHSQPEYSNGSWFAMLFTAGMGIGLMFFGVAEPVMHY
HHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
VNPPSGEPQTIEAAQQALRVTFFHWGLHAWAIYAVVGLALAYFAYRHNLPLKTRSALYPL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
IGKKIYGPWGDSIDIFATIGTVFGVATSLGFGVTQINSGLHYLFGIEQSTTTQVLLIIFV
HCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEECCCCHHHHHHHHHHH
SILASLSVFLGLDKGVKRLSELNLVLALILLVFVFIAGPSIYLLQTTIQNTGQYISNLFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHH
MTFNLYAYQPNGWIGGWTILYWAWWISWSPFVGMFIARVSRGRTIREFIVGVLLIPTGFT
HEEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHH
IIWMGFLGNAALFSIIHEHQTTLIQAVQQDSSVALFEFLGHLPWSGVMNILATILVVLFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VTSADSGALVTDYLTAKTENSPTWQRLFWTVLMAVLAIILLLVGGLAALQSSIIMSALPF
HHCCCCCCEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVVMLLMSWGLIKALHLDVTKMIAIQEARITPRAIHNPRSWQQRLGLIMHYPHSEEEVKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHCCEEEECCCCHHHHHH
YIEKQVSRAFENIQHEFRRRHLEVSISEVEDGMQLRVDHHHEINFIYKVVSRETVPPSFL
HHHHHHHHHHHHHHHHHHHHHHHEEHHHHCCCCEEEECCCCHHHHHHHHHHCCCCCCCCE
IGRTEAEDGQYFQAEVFLREGGQNYDVMDWTQEDLIQDIIDQYERHLHFLNIVRS
EECCCCCCCCEEEEEHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]