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The map label for this gene is pleD [H]

Identifier: 213159035

GI number: 213159035

Start: 3855100

End: 3856806

Strand: Reverse

Name: pleD [H]

Synonym: AB57_3743

Alternate gene names: 213159035

Gene position: 3856806-3855100 (Counterclockwise)

Preceding gene: 213159037

Following gene: 213159033

Centisome position: 95.22

GC content: 36.97

Gene sequence:

>1707_bases
ATGTCAGGATTACGTTCAGAACATATCAGTAATCGACTGTTTTTCTTCATGTTGGTCATTATTTTGTCCTTACTGTTTAT
GGCGGTTCCGCTTATTGTCAGCAGTTATCAAGAGTACTTAAAAACTAAACAGGCTTTGGTTGAAATTAAAAGCTTAAGAT
CAATTGCTGAAGTCGCAAATAAAGTATCTAAAGAACGTGCGCCCGCAAATAAATTAATGTCGAGTAACGCTGCCGACTTT
TTAAAAAATCAAAAAAACTTAAAAGAATATCGCCTCTCTGTTGATCGCCAACTTAATGAAACTATTCATATTTTAAAAGA
AGAAGGTTATACCGATTTAGCAAATACTTTAGATACTAAATTTAGAGATGATTTAAAACAGGCTCGTGCTGTAGTTGACT
ATTATGTTGCAACTCCTGAACAAGCGAGAAGCTCTATACAATTCGATAATGCAATTTTGGCTATGTTTGGAGCTTGGGAT
AGCTGCCGAGAAGTTTTACAAAATTTGATGCTACAGTTAAAAAGTAAAGATAGCCAAATTAACAATTACTTTACTTTGAT
TGTGGTGTTGACCGATATGCGGGATCAAGCTGGTCGTACCGCATCTAATGTTATTGCGCCTGTGACCTTTAAAGAAACCA
TACCTAATGATAATCGGGCGCGGTCTTTGCAGACTCAGCAGCAAGCACGCTACTTATGGGCACTTGTAGACACACTACAG
TCAGAGCAAGCTAAAACTCCGGAATATCATCGCCTTTACTCAAGGGTTAAAACTGAATTTTTAGATAAAGGCGTACCTAT
TGTGGGTCAGCTTTTAGATGAAAGCCAAAAGCACCAAGCCTATCATTTAAACGGAACAGAACTGACAGACCAAATCGTTG
ATAAATTTACCACGGTTATTGATTTGCAAAAGTTTTTACTTAATAAAAGCGTCTTGATTGCATCTCAAGAAAACTATTTG
GCAAAAAAGAAGTTTTTCCTTACTTTGCTCATTTCAAGCATATCCTTAGCAGCAGCGTTACTTACACTAATTTATGCAAA
GAGAAGGGTGTTTGAGCCTCTTATTCAGGCCCGTACCTTAATTCTTGATTTATCTCATAGCACAGATTACCCAATAGATG
AGTTGAAACCTAAAAAAGGCGAATTTATTTCGTTGTTCGAAGCTATTGACCGTTTACAAAGGATGCTCAAGCAGCGAGAT
GCTTTTGAATTCCAGCTTAAAAATATTGCCAATACAGATCCATTAACGGGTGTATCCAATCGTTTGGCTTTATCTGAGTA
TTTAAAAATTGTTGAGAGTTACCCTCAAAAATTTTCTCAAACATGTTTGATGATTATTGATATTGATCGCTTTAAACACG
TGAATGATCAATATGGTCATATTGTGGGTGACCAAGTGATTCGGCGTATCGCAGATCAATTAAAAGCAAATGTACGGGCT
TCTGATTTAATTGTTCGTTATGGTGGTGATGAGTTTTTAATTTTGTTAGAACAAGTGCAGTTTTTAGATGCACGTCTCTT
GGCCGAAAAAATTCGTATTGCGATTTCTTTAGAGGAAATTGATCTATCTGGTACAAAAGAAAAATTGCATGTATCGGTCA
GTATTGGGTTTGCGATTGGAGCGACAAGCTGGATGGAGTTATTAGAGAACGCTGATCGTTCACTCTTAAGAGCTAAAGCT
CGGGGACGGAATGTAGTAGAGGGATAA

Upstream 100 bases:

>100_bases
TGTTGCTGAACTTCTGTTCAGTAAAGTGCTAGGATGCGGATTAATTACTTCATTCTATAGTAGTGATCATGGTAGGACAT
TTACGGAGTACCAGCTAGAA

Downstream 100 bases:

>100_bases
AAAAAGCCGGAAAATAAATTCCGGCTTTTTGTTTTATTGTTTCAACATCGGTTTCAAGAAACGACCCGTATGGGAAATTT
CAACCTCAGCCACTTGTTCA

Product: GGDEF family protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MSGLRSEHISNRLFFFMLVIILSLLFMAVPLIVSSYQEYLKTKQALVEIKSLRSIAEVANKVSKERAPANKLMSSNAADF
LKNQKNLKEYRLSVDRQLNETIHILKEEGYTDLANTLDTKFRDDLKQARAVVDYYVATPEQARSSIQFDNAILAMFGAWD
SCREVLQNLMLQLKSKDSQINNYFTLIVVLTDMRDQAGRTASNVIAPVTFKETIPNDNRARSLQTQQQARYLWALVDTLQ
SEQAKTPEYHRLYSRVKTEFLDKGVPIVGQLLDESQKHQAYHLNGTELTDQIVDKFTTVIDLQKFLLNKSVLIASQENYL
AKKKFFLTLLISSISLAAALLTLIYAKRRVFEPLIQARTLILDLSHSTDYPIDELKPKKGEFISLFEAIDRLQRMLKQRD
AFEFQLKNIANTDPLTGVSNRLALSEYLKIVESYPQKFSQTCLMIIDIDRFKHVNDQYGHIVGDQVIRRIADQLKANVRA
SDLIVRYGGDEFLILLEQVQFLDARLLAEKIRIAISLEEIDLSGTKEKLHVSVSIGFAIGATSWMELLENADRSLLRAKA
RGRNVVEG

Sequences:

>Translated_568_residues
MSGLRSEHISNRLFFFMLVIILSLLFMAVPLIVSSYQEYLKTKQALVEIKSLRSIAEVANKVSKERAPANKLMSSNAADF
LKNQKNLKEYRLSVDRQLNETIHILKEEGYTDLANTLDTKFRDDLKQARAVVDYYVATPEQARSSIQFDNAILAMFGAWD
SCREVLQNLMLQLKSKDSQINNYFTLIVVLTDMRDQAGRTASNVIAPVTFKETIPNDNRARSLQTQQQARYLWALVDTLQ
SEQAKTPEYHRLYSRVKTEFLDKGVPIVGQLLDESQKHQAYHLNGTELTDQIVDKFTTVIDLQKFLLNKSVLIASQENYL
AKKKFFLTLLISSISLAAALLTLIYAKRRVFEPLIQARTLILDLSHSTDYPIDELKPKKGEFISLFEAIDRLQRMLKQRD
AFEFQLKNIANTDPLTGVSNRLALSEYLKIVESYPQKFSQTCLMIIDIDRFKHVNDQYGHIVGDQVIRRIADQLKANVRA
SDLIVRYGGDEFLILLEQVQFLDARLLAEKIRIAISLEEIDLSGTKEKLHVSVSIGFAIGATSWMELLENADRSLLRAKA
RGRNVVEG
>Mature_567_residues
SGLRSEHISNRLFFFMLVIILSLLFMAVPLIVSSYQEYLKTKQALVEIKSLRSIAEVANKVSKERAPANKLMSSNAADFL
KNQKNLKEYRLSVDRQLNETIHILKEEGYTDLANTLDTKFRDDLKQARAVVDYYVATPEQARSSIQFDNAILAMFGAWDS
CREVLQNLMLQLKSKDSQINNYFTLIVVLTDMRDQAGRTASNVIAPVTFKETIPNDNRARSLQTQQQARYLWALVDTLQS
EQAKTPEYHRLYSRVKTEFLDKGVPIVGQLLDESQKHQAYHLNGTELTDQIVDKFTTVIDLQKFLLNKSVLIASQENYLA
KKKFFLTLLISSISLAAALLTLIYAKRRVFEPLIQARTLILDLSHSTDYPIDELKPKKGEFISLFEAIDRLQRMLKQRDA
FEFQLKNIANTDPLTGVSNRLALSEYLKIVESYPQKFSQTCLMIIDIDRFKHVNDQYGHIVGDQVIRRIADQLKANVRAS
DLIVRYGGDEFLILLEQVQFLDARLLAEKIRIAISLEEIDLSGTKEKLHVSVSIGFAIGATSWMELLENADRSLLRAKAR
GRNVVEG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=320, Percent_Identity=29.375, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI87081881, Length=179, Percent_Identity=35.195530726257, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI145693134, Length=172, Percent_Identity=34.8837209302326, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1787802, Length=184, Percent_Identity=33.1521739130435, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI87082007, Length=183, Percent_Identity=31.6939890710383, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786584, Length=167, Percent_Identity=32.9341317365269, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=31.4465408805031, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1788085, Length=172, Percent_Identity=31.3953488372093, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1788381, Length=175, Percent_Identity=30.2857142857143, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788956, Length=252, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787541, Length=169, Percent_Identity=28.4023668639053, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787056, Length=172, Percent_Identity=30.8139534883721, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87081974, Length=86, Percent_Identity=38.3720930232558, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 64838; Mature: 64707

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLRSEHISNRLFFFMLVIILSLLFMAVPLIVSSYQEYLKTKQALVEIKSLRSIAEVAN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVSKERAPANKLMSSNAADFLKNQKNLKEYRLSVDRQLNETIHILKEEGYTDLANTLDTK
HHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
FRDDLKQARAVVDYYVATPEQARSSIQFDNAILAMFGAWDSCREVLQNLMLQLKSKDSQI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHH
NNYFTLIVVLTDMRDQAGRTASNVIAPVTFKETIPNDNRARSLQTQQQARYLWALVDTLQ
HHHEEEEEEEEHHHHHHCCCHHHCEECEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
SEQAKTPEYHRLYSRVKTEFLDKGVPIVGQLLDESQKHQAYHLNGTELTDQIVDKFTTVI
HHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCHHCCCEEECCCHHHHHHHHHHHHHHH
DLQKFLLNKSVLIASQENYLAKKKFFLTLLISSISLAAALLTLIYAKRRVFEPLIQARTL
HHHHHHHCCHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILDLSHSTDYPIDELKPKKGEFISLFEAIDRLQRMLKQRDAFEFQLKNIANTDPLTGVSN
HEECCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHH
RLALSEYLKIVESYPQKFSQTCLMIIDIDRFKHVNDQYGHIVGDQVIRRIADQLKANVRA
HHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCH
SDLIVRYGGDEFLILLEQVQFLDARLLAEKIRIAISLEEIDLSGTKEKLHVSVSIGFAIG
HHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEEEECHHHH
ATSWMELLENADRSLLRAKARGRNVVEG
HHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SGLRSEHISNRLFFFMLVIILSLLFMAVPLIVSSYQEYLKTKQALVEIKSLRSIAEVAN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVSKERAPANKLMSSNAADFLKNQKNLKEYRLSVDRQLNETIHILKEEGYTDLANTLDTK
HHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
FRDDLKQARAVVDYYVATPEQARSSIQFDNAILAMFGAWDSCREVLQNLMLQLKSKDSQI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHH
NNYFTLIVVLTDMRDQAGRTASNVIAPVTFKETIPNDNRARSLQTQQQARYLWALVDTLQ
HHHEEEEEEEEHHHHHHCCCHHHCEECEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
SEQAKTPEYHRLYSRVKTEFLDKGVPIVGQLLDESQKHQAYHLNGTELTDQIVDKFTTVI
HHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCHHCCCEEECCCHHHHHHHHHHHHHHH
DLQKFLLNKSVLIASQENYLAKKKFFLTLLISSISLAAALLTLIYAKRRVFEPLIQARTL
HHHHHHHCCHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILDLSHSTDYPIDELKPKKGEFISLFEAIDRLQRMLKQRDAFEFQLKNIANTDPLTGVSN
HEECCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHH
RLALSEYLKIVESYPQKFSQTCLMIIDIDRFKHVNDQYGHIVGDQVIRRIADQLKANVRA
HHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCH
SDLIVRYGGDEFLILLEQVQFLDARLLAEKIRIAISLEEIDLSGTKEKLHVSVSIGFAIG
HHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEEEECHHHH
ATSWMELLENADRSLLRAKARGRNVVEG
HHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA