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The map label for this gene is hisH [H]

Identifier: 213158981

GI number: 213158981

Start: 3802782

End: 3803399

Strand: Direct

Name: hisH [H]

Synonym: AB57_3683

Alternate gene names: 213158981

Gene position: 3802782-3803399 (Clockwise)

Preceding gene: 213158980

Following gene: 213158982

Centisome position: 93.88

GC content: 43.2

Gene sequence:

>618_bases
ATGACACGTATCGCATTGCTTGATTATGGCATGGGTAATCTGCACTCAGCCGCTAAAGCACTTGAATATGTAGGTGCAAC
CGTCGATGTGACAAATGATCCAAAATTAATTGCACAAGCCGACAAAATTGTTTTCCCGGGTGTTGGGGCAATGCGTGACT
GTATGCAAGGCATGCGTGAAGCAGGTATTGATGAAGTTGTACGTAAAGCAGCTTTTAATAAGCCTGTTCTTGCAATTTGC
GTAGGCATGCAGGCACTACTACAAAGCTCAGAAGAAAACGGCGGTGTAGATGCCTTAGGTATTTTTGACGGGATAGTAAA
ACACTTCCCTCAAATGGAAGGTTTAAAAGTCCCACACATGGGCTGGAACCAAGTTCACCAAATGGACCCAAGCCACCCGA
TGTGGAACAACATTGAACAAGATGCCCGCTTTTACTTTGTACACAGCTACTATGTTGAACCTAAAGATGAAAATCTTGTG
GCTGCCACATGTGAGTATGGCGTGAACTTCTGCACAGCAATTCACAAAGATAATTTATTCGCGACTCAATTCCACCCAGA
GAAAAGTCACACTGCTGGTTTGCAGTTACTGAAAAACTTTGTGGAATGGAATATTTAA

Upstream 100 bases:

>100_bases
TCGAGAGTGTATTTAAAGCACTAGCTCGTGCTTTACGTATGGCATGTGAAATTGATCCACGTGCTGAAAACACGATTGCA
TCGACAAAAGGTAGCCTCTA

Downstream 100 bases:

>100_bases
TTCGATTCAAAAACTAAAACAGATCAGGTCAAGATAGCCTGATCTGTTTTTTTATTTATAATAATAACCAATCATTTATA
GAATGATATTTTATTCAACA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MTRIALLDYGMGNLHSAAKALEYVGATVDVTNDPKLIAQADKIVFPGVGAMRDCMQGMREAGIDEVVRKAAFNKPVLAIC
VGMQALLQSSEENGGVDALGIFDGIVKHFPQMEGLKVPHMGWNQVHQMDPSHPMWNNIEQDARFYFVHSYYVEPKDENLV
AATCEYGVNFCTAIHKDNLFATQFHPEKSHTAGLQLLKNFVEWNI

Sequences:

>Translated_205_residues
MTRIALLDYGMGNLHSAAKALEYVGATVDVTNDPKLIAQADKIVFPGVGAMRDCMQGMREAGIDEVVRKAAFNKPVLAIC
VGMQALLQSSEENGGVDALGIFDGIVKHFPQMEGLKVPHMGWNQVHQMDPSHPMWNNIEQDARFYFVHSYYVEPKDENLV
AATCEYGVNFCTAIHKDNLFATQFHPEKSHTAGLQLLKNFVEWNI
>Mature_204_residues
TRIALLDYGMGNLHSAAKALEYVGATVDVTNDPKLIAQADKIVFPGVGAMRDCMQGMREAGIDEVVRKAAFNKPVLAICV
GMQALLQSSEENGGVDALGIFDGIVKHFPQMEGLKVPHMGWNQVHQMDPSHPMWNNIEQDARFYFVHSYYVEPKDENLVA
ATCEYGVNFCTAIHKDNLFATQFHPEKSHTAGLQLLKNFVEWNI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=201, Percent_Identity=42.2885572139304, Blast_Score=153, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6319725, Length=214, Percent_Identity=33.6448598130841, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 22810; Mature: 22679

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIALLDYGMGNLHSAAKALEYVGATVDVTNDPKLIAQADKIVFPGVGAMRDCMQGMRE
CCEEEEEECCCCHHHHHHHHHHHHCCEEEECCCCHHHHHCCCEECCCCHHHHHHHHHHHH
AGIDEVVRKAAFNKPVLAICVGMQALLQSSEENGGVDALGIFDGIVKHFPQMEGLKVPHM
CCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCC
GWNQVHQMDPSHPMWNNIEQDARFYFVHSYYVEPKDENLVAATCEYGVNFCTAIHKDNLF
CHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEHHHHCCHHHHHHHCCCEE
ATQFHPEKSHTAGLQLLKNFVEWNI
EEEECCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRIALLDYGMGNLHSAAKALEYVGATVDVTNDPKLIAQADKIVFPGVGAMRDCMQGMRE
CEEEEEECCCCHHHHHHHHHHHHCCEEEECCCCHHHHHCCCEECCCCHHHHHHHHHHHH
AGIDEVVRKAAFNKPVLAICVGMQALLQSSEENGGVDALGIFDGIVKHFPQMEGLKVPHM
CCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCC
GWNQVHQMDPSHPMWNNIEQDARFYFVHSYYVEPKDENLVAATCEYGVNFCTAIHKDNLF
CHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEHHHHCCHHHHHHHCCCEE
ATQFHPEKSHTAGLQLLKNFVEWNI
EEEECCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA