Click here to switch to the map view.

The map label for this gene is murF [H]

Identifier: 213158952

GI number: 213158952

Start: 3763311

End: 3764711

Strand: Reverse

Name: murF [H]

Synonym: AB57_3653

Alternate gene names: 213158952

Gene position: 3764711-3763311 (Counterclockwise)

Preceding gene: 213158953

Following gene: 213158951

Centisome position: 92.94

GC content: 43.75

Gene sequence:

>1401_bases
ATGCATACTTCAACCACCAGTACCGTGCCTTTGGAACCGTGGACAGCTCAACAATTACAACAGGCAACACAGGGTTACTG
GCATAAAGACCAGATCCCTCAAACTGAAATTAAACGTATTTTGACTGACTCGCGTCATGCAGAAAGTGGGGATGCCTTTT
TAGCACTAAAAGGTGAACGTTTCGACGCGCATAATTTTGTCGCGCAAGTGGTTGCAAATGGTTGCCAAGTCGCTATTGTC
GAGCGGCCTATAGATGCAGAAATTGCTCAGCTTGTAGTTGCCGATACCCGTTTGGCTTTGGGGCAGCTTGGTGCGTATCG
TCGTGAACAAAATGCTCAATTGAAAGTAATTGCTTTAACGGGCAGTAGTGGTAAAACCACCACCAAAGAAATGCTAGGAA
GTATTTTGTCTCGTTTAGCACCTACATTGATTACCCGAGGCAATTTAAATAATGACCTTGGTGTGCCGATGATGTTGCTT
GAGCTTCGAAAGGAGCATCAGTATGCGGTGATGGAACTTGGTGCAAATCATCAAGGTGAAATTGATTACACATCAAAAAT
TGTTCAACCGCATGTTGCTGGCATTTTAAATATTGGAACTGCTCATTTGGGTGAGTTCGGCGGGCGTGATGGCATTTGCC
GCGCTAAATCAGAAATCTATCGCCATATTTTACCTCAGGGCGTTGCTATTGTTCCGCAACAAGATGACTTTACCGCTGAG
ATTCGTGAAGCAGCCAAAAGCCATCAAATCATGAGCTTTGGTGCTGGTGGTGATGTCTTTGCTACAGAGATTGAATTGTT
GCCACAATCTGCCAATTTCCAGTTACATACGCCTCAAGGTTCAAGTTTTGTAAGACTGCCGTTTGCGGGTGAGCACAATG
TGCAAAACGCAACTGCAGCAGTAGCATTTGCTTTGGCGTTAGGTGTTAGCTTGGAAGATATCGTAAAAGGTCTAGAGCAA
GCACAAGGTGCAAAAGGTCGTTTGAATTTTATTCAAAAAACGCCTCATTTATTTATTGATGATACTTATAACGCCAATCC
AACATCTATGCGTGCTGCGGCACAAGTATTACTTCAGCAAAATGGCATTAAAGTCATGGTGATGGGGGATATTGGGGAGT
TAGGTGATAGCAGTTGGCAAGAACATCACGATTTAGGTCGCGATCTTGCTGAGCTTCCTTTAGACCATATTGTTGCAGTT
GGGCAGTTTGCCTCAGCAGCTCTCGAAGGCGCAGGTCTTCATTCTACTAAATTGAAAGCATTTCAGACTCAAGCTGAGGC
GCTTCCATTTTTAATCAATCTAATCCAAACACATCAACCCCAGTCCATGAGTTTCCTGTTTAAAGGTTCTCGCTTTACTC
ATATGGAAACGTTGATGGCTGATTTGATGGAGAAACTTTAA

Upstream 100 bases:

>100_bases
GTGTGCGTCACTGGTTTGATGATGTGGTTGAAGTGCGTTCCGCGATTGATGCCCAACATCATACGGTAGATGCTGCTTAC
CCTGCACAATAGGAAAAATT

Downstream 100 bases:

>100_bases
ATGCTGTTATGGTTGTTTGAACAACTTGCGGGCTATCACAGTTCGTTTCAGGTTGTTCGTTATTTAACATTACGTTCTTT
ACTCAGTGTATTAACGTCAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MHTSTTSTVPLEPWTAQQLQQATQGYWHKDQIPQTEIKRILTDSRHAESGDAFLALKGERFDAHNFVAQVVANGCQVAIV
ERPIDAEIAQLVVADTRLALGQLGAYRREQNAQLKVIALTGSSGKTTTKEMLGSILSRLAPTLITRGNLNNDLGVPMMLL
ELRKEHQYAVMELGANHQGEIDYTSKIVQPHVAGILNIGTAHLGEFGGRDGICRAKSEIYRHILPQGVAIVPQQDDFTAE
IREAAKSHQIMSFGAGGDVFATEIELLPQSANFQLHTPQGSSFVRLPFAGEHNVQNATAAVAFALALGVSLEDIVKGLEQ
AQGAKGRLNFIQKTPHLFIDDTYNANPTSMRAAAQVLLQQNGIKVMVMGDIGELGDSSWQEHHDLGRDLAELPLDHIVAV
GQFASAALEGAGLHSTKLKAFQTQAEALPFLINLIQTHQPQSMSFLFKGSRFTHMETLMADLMEKL

Sequences:

>Translated_466_residues
MHTSTTSTVPLEPWTAQQLQQATQGYWHKDQIPQTEIKRILTDSRHAESGDAFLALKGERFDAHNFVAQVVANGCQVAIV
ERPIDAEIAQLVVADTRLALGQLGAYRREQNAQLKVIALTGSSGKTTTKEMLGSILSRLAPTLITRGNLNNDLGVPMMLL
ELRKEHQYAVMELGANHQGEIDYTSKIVQPHVAGILNIGTAHLGEFGGRDGICRAKSEIYRHILPQGVAIVPQQDDFTAE
IREAAKSHQIMSFGAGGDVFATEIELLPQSANFQLHTPQGSSFVRLPFAGEHNVQNATAAVAFALALGVSLEDIVKGLEQ
AQGAKGRLNFIQKTPHLFIDDTYNANPTSMRAAAQVLLQQNGIKVMVMGDIGELGDSSWQEHHDLGRDLAELPLDHIVAV
GQFASAALEGAGLHSTKLKAFQTQAEALPFLINLIQTHQPQSMSFLFKGSRFTHMETLMADLMEKL
>Mature_466_residues
MHTSTTSTVPLEPWTAQQLQQATQGYWHKDQIPQTEIKRILTDSRHAESGDAFLALKGERFDAHNFVAQVVANGCQVAIV
ERPIDAEIAQLVVADTRLALGQLGAYRREQNAQLKVIALTGSSGKTTTKEMLGSILSRLAPTLITRGNLNNDLGVPMMLL
ELRKEHQYAVMELGANHQGEIDYTSKIVQPHVAGILNIGTAHLGEFGGRDGICRAKSEIYRHILPQGVAIVPQQDDFTAE
IREAAKSHQIMSFGAGGDVFATEIELLPQSANFQLHTPQGSSFVRLPFAGEHNVQNATAAVAFALALGVSLEDIVKGLEQ
AQGAKGRLNFIQKTPHLFIDDTYNANPTSMRAAAQVLLQQNGIKVMVMGDIGELGDSSWQEHHDLGRDLAELPLDHIVAV
GQFASAALEGAGLHSTKLKAFQTQAEALPFLINLIQTHQPQSMSFLFKGSRFTHMETLMADLMEKL

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=432, Percent_Identity=41.6666666666667, Blast_Score=286, Evalue=2e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50798; Mature: 50798

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTSTTSTVPLEPWTAQQLQQATQGYWHKDQIPQTEIKRILTDSRHAESGDAFLALKGER
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCC
FDAHNFVAQVVANGCQVAIVERPIDAEIAQLVVADTRLALGQLGAYRREQNAQLKVIALT
CCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEE
GSSGKTTTKEMLGSILSRLAPTLITRGNLNNDLGVPMMLLELRKEHQYAVMELGANHQGE
CCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHCEEEEHCCCCCCCC
IDYTSKIVQPHVAGILNIGTAHLGEFGGRDGICRAKSEIYRHILPQGVAIVPQQDDFTAE
CCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHH
IREAAKSHQIMSFGAGGDVFATEIELLPQSANFQLHTPQGSSFVRLPFAGEHNVQNATAA
HHHHHHHCCEEEECCCCCEEEEEHEECCCCCCEEEECCCCCCEEEECCCCCCCCCHHHHH
VAFALALGVSLEDIVKGLEQAQGAKGRLNFIQKTPHLFIDDTYNANPTSMRAAAQVLLQQ
HHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHC
NGIKVMVMGDIGELGDSSWQEHHDLGRDLAELPLDHIVAVGQFASAALEGAGLHSTKLKA
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHH
FQTQAEALPFLINLIQTHQPQSMSFLFKGSRFTHMETLMADLMEKL
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHC
>Mature Secondary Structure
MHTSTTSTVPLEPWTAQQLQQATQGYWHKDQIPQTEIKRILTDSRHAESGDAFLALKGER
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCC
FDAHNFVAQVVANGCQVAIVERPIDAEIAQLVVADTRLALGQLGAYRREQNAQLKVIALT
CCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEE
GSSGKTTTKEMLGSILSRLAPTLITRGNLNNDLGVPMMLLELRKEHQYAVMELGANHQGE
CCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHCEEEEHCCCCCCCC
IDYTSKIVQPHVAGILNIGTAHLGEFGGRDGICRAKSEIYRHILPQGVAIVPQQDDFTAE
CCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHH
IREAAKSHQIMSFGAGGDVFATEIELLPQSANFQLHTPQGSSFVRLPFAGEHNVQNATAA
HHHHHHHCCEEEECCCCCEEEEEHEECCCCCCEEEECCCCCCEEEECCCCCCCCCHHHHH
VAFALALGVSLEDIVKGLEQAQGAKGRLNFIQKTPHLFIDDTYNANPTSMRAAAQVLLQQ
HHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHC
NGIKVMVMGDIGELGDSSWQEHHDLGRDLAELPLDHIVAVGQFASAALEGAGLHSTKLKA
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHH
FQTQAEALPFLINLIQTHQPQSMSFLFKGSRFTHMETLMADLMEKL
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]