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The map label for this gene is yafC [H]

Identifier: 213158760

GI number: 213158760

Start: 4016441

End: 4017343

Strand: Reverse

Name: yafC [H]

Synonym: AB57_3894

Alternate gene names: 213158760

Gene position: 4017343-4016441 (Counterclockwise)

Preceding gene: 213158764

Following gene: 213158758

Centisome position: 99.18

GC content: 39.31

Gene sequence:

>903_bases
ATGAAATCAACAATTGAAGAACTTGTTGCCTTTATCACAATTGTTGATACAGGTTCCTTTGTGGCAGCAGCAGAACATTT
AAAGCAAACACCTTCGGGGGTGAGCCGATCTTTAACCCGTTTGGAAGCAAAGTTAGATGTGACTTTGCTAGAGCGAACAA
CGCGTAAATTGAAACTGACTCAAGAAGGGCAACAATTTCTTATAAAGGCCCGTAAAATTTTAAATGAGCTGAATGCGGCC
GAAGAAGAACTTCAAAAGTCTGATCAAGGTACTGCCGGTCTTATTCGGGTTGATTCTGCCACACCGTTTGTTTTGCACGT
GATTGCACCATTAATGCATAAATTCCGTGAGTGTTACCCCGATATTGAAATCGAATTAAATAGCAATGATCAGGTGATTG
ACTTACTTCAGCATAAAACCGATGTCGCATTTCGATTTGGGGAACTTAATGACTCTAGTCTGCACGCCAAATTGGTATGT
AAGAGCCGTTTATATATTGTGGCAAGTCCTGACTATCTAGCTCTTAAGGGAACGCCAACACAACCTGAAGAACTTGAACA
GCATGATTTGATTGGCTTTACTAGACCTACTTATATCAATAGTTGGCCAATTAAAGTTGGCGATGAATATTTTTTTGCCC
AATCAAAAATTAAAGCATCGAGTGGAGAGACCGTACGCCAACTGACGATTAGAGGACACGGCATTGCGCGACTATCTGAA
TTTGAAATTTGGAAAGATATGGAAGAAGGGCGATTGATTGCATTATTTGAAGACAAAATTGAGCATCAATATCAAAGCAT
CCATGCTGTGTATTATCAACAAGAGCATCTACCAAAACGAATCCGTTTATTCATTGAATTTTTGGCAGAACAGTTAAAAG
ATGGTTTTAAAAACGCTCTCTAA

Upstream 100 bases:

>100_bases
AATTGTTTAACTTGAGGTGCATTTTGCTTTTTTTATTGTTGCGAAAAAATAGATGAATAAGCAAAATATCTTTGATGTTT
AGTCAATAATAGAATAAACC

Downstream 100 bases:

>100_bases
AGGTCGTATAACTATCGGTTGGAATATGAACTTCTAAAATAGGTGTCGTTTGTCCTTCTGCCGAATGTTGAGACAGAAAA
TAGCTTTTTTGCTGTTCTCG

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKSTIEELVAFITIVDTGSFVAAAEHLKQTPSGVSRSLTRLEAKLDVTLLERTTRKLKLTQEGQQFLIKARKILNELNAA
EEELQKSDQGTAGLIRVDSATPFVLHVIAPLMHKFRECYPDIEIELNSNDQVIDLLQHKTDVAFRFGELNDSSLHAKLVC
KSRLYIVASPDYLALKGTPTQPEELEQHDLIGFTRPTYINSWPIKVGDEYFFAQSKIKASSGETVRQLTIRGHGIARLSE
FEIWKDMEEGRLIALFEDKIEHQYQSIHAVYYQQEHLPKRIRLFIEFLAEQLKDGFKNAL

Sequences:

>Translated_300_residues
MKSTIEELVAFITIVDTGSFVAAAEHLKQTPSGVSRSLTRLEAKLDVTLLERTTRKLKLTQEGQQFLIKARKILNELNAA
EEELQKSDQGTAGLIRVDSATPFVLHVIAPLMHKFRECYPDIEIELNSNDQVIDLLQHKTDVAFRFGELNDSSLHAKLVC
KSRLYIVASPDYLALKGTPTQPEELEQHDLIGFTRPTYINSWPIKVGDEYFFAQSKIKASSGETVRQLTIRGHGIARLSE
FEIWKDMEEGRLIALFEDKIEHQYQSIHAVYYQQEHLPKRIRLFIEFLAEQLKDGFKNAL
>Mature_300_residues
MKSTIEELVAFITIVDTGSFVAAAEHLKQTPSGVSRSLTRLEAKLDVTLLERTTRKLKLTQEGQQFLIKARKILNELNAA
EEELQKSDQGTAGLIRVDSATPFVLHVIAPLMHKFRECYPDIEIELNSNDQVIDLLQHKTDVAFRFGELNDSSLHAKLVC
KSRLYIVASPDYLALKGTPTQPEELEQHDLIGFTRPTYINSWPIKVGDEYFFAQSKIKASSGETVRQLTIRGHGIARLSE
FEIWKDMEEGRLIALFEDKIEHQYQSIHAVYYQQEHLPKRIRLFIEFLAEQLKDGFKNAL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=301, Percent_Identity=43.1893687707641, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=27.891156462585, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789440, Length=287, Percent_Identity=29.616724738676, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI87081978, Length=297, Percent_Identity=26.5993265993266, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1789639, Length=290, Percent_Identity=25.5172413793103, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1787589, Length=289, Percent_Identity=26.643598615917, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787128, Length=296, Percent_Identity=23.3108108108108, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789173, Length=240, Percent_Identity=28.75, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI145693105, Length=195, Percent_Identity=24.1025641025641, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786448, Length=311, Percent_Identity=25.08038585209, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788481, Length=298, Percent_Identity=24.1610738255034, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082132, Length=300, Percent_Identity=23.6666666666667, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786508, Length=258, Percent_Identity=26.7441860465116, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788706, Length=292, Percent_Identity=26.027397260274, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI157672245, Length=169, Percent_Identity=29.585798816568, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788748, Length=175, Percent_Identity=30.2857142857143, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082024, Length=117, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788297, Length=239, Percent_Identity=25.5230125523013, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34269; Mature: 34269

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSTIEELVAFITIVDTGSFVAAAEHLKQTPSGVSRSLTRLEAKLDVTLLERTTRKLKLT
CCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEH
QEGQQFLIKARKILNELNAAEEELQKSDQGTAGLIRVDSATPFVLHVIAPLMHKFRECYP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCC
DIEIELNSNDQVIDLLQHKTDVAFRFGELNDSSLHAKLVCKSRLYIVASPDYLALKGTPT
CEEEEECCCCHHHHHHHHHHHHEEEECCCCCCCHHEEEEEECEEEEEECCCEEEEECCCC
QPEELEQHDLIGFTRPTYINSWPIKVGDEYFFAQSKIKASSGETVRQLTIRGHGIARLSE
CHHHHHHHCCCCCCCCCEECCCCCEECCCEEEHHHHHCCCCCCCEEEEEECCCCCHHHHH
FEIWKDMEEGRLIALFEDKIEHQYQSIHAVYYQQEHLPKRIRLFIEFLAEQLKDGFKNAL
HHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSTIEELVAFITIVDTGSFVAAAEHLKQTPSGVSRSLTRLEAKLDVTLLERTTRKLKLT
CCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEH
QEGQQFLIKARKILNELNAAEEELQKSDQGTAGLIRVDSATPFVLHVIAPLMHKFRECYP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCC
DIEIELNSNDQVIDLLQHKTDVAFRFGELNDSSLHAKLVCKSRLYIVASPDYLALKGTPT
CEEEEECCCCHHHHHHHHHHHHEEEECCCCCCCHHEEEEEECEEEEEECCCEEEEECCCC
QPEELEQHDLIGFTRPTYINSWPIKVGDEYFFAQSKIKASSGETVRQLTIRGHGIARLSE
CHHHHHHHCCCCCCCCCEECCCCCEECCCEEEHHHHHCCCCCCCEEEEEECCCCCHHHHH
FEIWKDMEEGRLIALFEDKIEHQYQSIHAVYYQQEHLPKRIRLFIEFLAEQLKDGFKNAL
HHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]