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The map label for this gene is pncA [H]

Identifier: 213158741

GI number: 213158741

Start: 4001198

End: 4001842

Strand: Reverse

Name: pncA [H]

Synonym: AB57_3875

Alternate gene names: 213158741

Gene position: 4001842-4001198 (Counterclockwise)

Preceding gene: 213158747

Following gene: 213158740

Centisome position: 98.8

GC content: 42.33

Gene sequence:

>645_bases
ATGAAAATGAATAAACAACCACAAAATTCGGCTTTAGTCGTAGTTGACGTGCAAAATGGTTTTACCCCTGGTGGAAACCT
AGCAGTTGCCGACGCCGATACGATTATTCCAACCATTAACCAATTGGCAGGTTGTTTTGAAAATGTAGTCCTCACTCAAG
ACTGGCATCCGGATAATCACATTTCTTTTGCAGCCAACCATCCGGGCAAGCAGCCATTTGAAACGATTGAACTGGATTAT
GGATCACAGGTGCTTTGGCCTAAGCATTGTATACAAGGCACTCATGATGCCGAATTTCATCCGGATTTAAATATCCCGAC
AGCTCAACTGATTATCCGAAAAGGTTTTCATGCTCATATTGATAGTTACTCGGCTTTTATGGAAGCCGACCACACCACAA
TGACAGGTTTAACGGGTTACTTAAAAGAACGTGGTATTGATACGGTCTATGTGGTCGGAATTGCCACCGACTTTTGTGTC
GCATGGACTGCTTTAGATGCGGTTAAGCAAGGCTTTAAAACTTTAGTGATTGAAGATGCGTGCAAGGGTATTGATCTTAA
TGGTTCATTAGAACAAGCGTGGCAAACTATGCAGCAACAAGGTGTTGTTCGTATTCAATCGACTGATCTGTTGAATGAAT
GCTAG

Upstream 100 bases:

>100_bases
AAAATGAGAATATGGTGCAATTCTTCCAACCCCTACTTAACACGGTCTTTTTTATGGCAAGATAAAATGCATATTTTTCC
CGAATGGGACTTAAGATGGC

Downstream 100 bases:

>100_bases
AGATACTGATTTTGATGCTTGGACTTTAATTGCGATTTGGTATGGATGAACAGTATGTCTGCATTACTCCGTTTTACTCA
GTTTGTTCAAAAGACGTTTG

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MKMNKQPQNSALVVVDVQNGFTPGGNLAVADADTIIPTINQLAGCFENVVLTQDWHPDNHISFAANHPGKQPFETIELDY
GSQVLWPKHCIQGTHDAEFHPDLNIPTAQLIIRKGFHAHIDSYSAFMEADHTTMTGLTGYLKERGIDTVYVVGIATDFCV
AWTALDAVKQGFKTLVIEDACKGIDLNGSLEQAWQTMQQQGVVRIQSTDLLNEC

Sequences:

>Translated_214_residues
MKMNKQPQNSALVVVDVQNGFTPGGNLAVADADTIIPTINQLAGCFENVVLTQDWHPDNHISFAANHPGKQPFETIELDY
GSQVLWPKHCIQGTHDAEFHPDLNIPTAQLIIRKGFHAHIDSYSAFMEADHTTMTGLTGYLKERGIDTVYVVGIATDFCV
AWTALDAVKQGFKTLVIEDACKGIDLNGSLEQAWQTMQQQGVVRIQSTDLLNEC
>Mature_214_residues
MKMNKQPQNSALVVVDVQNGFTPGGNLAVADADTIIPTINQLAGCFENVVLTQDWHPDNHISFAANHPGKQPFETIELDY
GSQVLWPKHCIQGTHDAEFHPDLNIPTAQLIIRKGFHAHIDSYSAFMEADHTTMTGLTGYLKERGIDTVYVVGIATDFCV
AWTALDAVKQGFKTLVIEDACKGIDLNGSLEQAWQTMQQQGVVRIQSTDLLNEC

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=180, Percent_Identity=40.5555555555556, Blast_Score=145, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI193206735, Length=219, Percent_Identity=34.703196347032, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17543130, Length=219, Percent_Identity=34.703196347032, Blast_Score=118, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71999339, Length=221, Percent_Identity=31.2217194570136, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6321401, Length=191, Percent_Identity=35.0785340314136, Blast_Score=111, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648247, Length=222, Percent_Identity=32.8828828828829, Blast_Score=120, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 23566; Mature: 23566

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMNKQPQNSALVVVDVQNGFTPGGNLAVADADTIIPTINQLAGCFENVVLTQDWHPDNH
CCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCEEEECCCCCCC
ISFAANHPGKQPFETIELDYGSQVLWPKHCIQGTHDAEFHPDLNIPTAQLIIRKGFHAHI
EEEEECCCCCCCCEEEEECCCCCEECHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCHHHH
DSYSAFMEADHTTMTGLTGYLKERGIDTVYVVGIATDFCVAWTALDAVKQGFKTLVIEDA
HHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHEEEHHH
CKGIDLNGSLEQAWQTMQQQGVVRIQSTDLLNEC
HCCCCCCCCHHHHHHHHHHCCCEEEECCHHHHCC
>Mature Secondary Structure
MKMNKQPQNSALVVVDVQNGFTPGGNLAVADADTIIPTINQLAGCFENVVLTQDWHPDNH
CCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCEEEECCCCCCC
ISFAANHPGKQPFETIELDYGSQVLWPKHCIQGTHDAEFHPDLNIPTAQLIIRKGFHAHI
EEEEECCCCCCCCEEEEECCCCCEECHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCHHHH
DSYSAFMEADHTTMTGLTGYLKERGIDTVYVVGIATDFCVAWTALDAVKQGFKTLVIEDA
HHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHEEEHHH
CKGIDLNGSLEQAWQTMQQQGVVRIQSTDLLNEC
HCCCCCCCCHHHHHHHHHHCCCEEEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]