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The map label for this gene is dctA [H]

Identifier: 213158680

GI number: 213158680

Start: 2723249

End: 2724607

Strand: Reverse

Name: dctA [H]

Synonym: AB57_2634

Alternate gene names: 213158680

Gene position: 2724607-2723249 (Counterclockwise)

Preceding gene: 213158682

Following gene: 213158679

Centisome position: 67.27

GC content: 39.22

Gene sequence:

>1359_bases
ATGGCTAAAAAACCGATTTATAAATCACTTTATTTTCAAGTGATTATTGCGATTATTGCGGGTGTTTTGGTAGGGCATTT
CTCACCAAGTACAACGCAAATTGTTAATGGTGTTGAACAACATATTCCAGGTTTAGGTGAGCAACTCAAGCCATTAGGTG
ATGCTTTTATTCGTTTGATTAAAATGATCATCGCACCTGTTATTTTCTGTACAGTAGTAAGTGGTATTGCTGGTATGGAA
AGCATGAAGTCAGTTGGTAAAACTGGCGGTGTTGCGTTGTTATACTTCGAGATCGTTTCAACAATCGCGCTTCTGATTGG
CCTTGTGGTGATTAACATTGCAAAGCCGGGCGTAGGAATGAATGTTGATCCAGCAACTCTGGACACTTCAGGTATCCAAA
AATACGTCACTTCTGGTGAGTCACAATCTTCCATTGATTTCTTGATGCACATCATCCCGGATACGGTGGTTGGTGCATTT
GCAAATGGTGAAATCTTACAGGTCCTTCTTTTTGCAATACTATTTGGTTTTGCACTTCATAAGTTAGGTGATGCAGGCCG
CCCAGTTTTAAAGTTAATTGATCAAATTGCACACGTGTTCTTTAACATTGTGAATATGATTATGAAGCTTGCGCCAATTG
GTGCATTTGGGGCAATGGCGTTTACTATCGGTAAATATGGTGTAGGTTCACTGGTACAGTTAGGCCAGCTCATTATCTGC
TTCTATATTACGTGTTTACTCTTTATTTTCTTAATTTTGGGTACAATCAGCCGCATTAGTGGATTCAGTATTCTGAAAAT
GATTCGCCTGATTCGTGAAGAACTATTAATTGTACTTGGTACATCTTCTTCTGAATCTGTTTTGCCTCGCATGTTACGTA
AACTCGAAATTGCAGGTTGTGAAAAGTCGGTTGTTGGTTTAGTTATTCCAACAGGTTATTCATTTAACCTTGATGGTACA
TCAATCTATTTGACTATGGCTGCAATCTTTATTGCTCAGGCAACGAATACACAGCTTGATATTCAACATCAGATTACTTT
GTTGTTGGTACTCCTAATCTCATCTAAAGGTGCAGCGGGTGTAACAGGCTCTGGCTTTATTGTTATGGCAGCAACTTTAT
CTGCAGTAGGACATATTCCGGTAGCTGGTTTGGCATTGATTTTAGGTATTGACCGCTTCATGTCAGAAGCACGTGCTTTA
ACCAATCTTGTTGGTAACTCTTTGGCAACGATTGTAGTTGCAAAATGGGTGGGTGCTTTAGATAAAGATAAGCTCAATGA
TGCTTTAAACAACCCAGATGAAGTTGACAGAAAAATGTTGGCTCCAAAAAATCCTCAGGTTGCAGAAGGTTTAGATTAA

Upstream 100 bases:

>100_bases
GCGGTGTTCATGTGAAAGGTTAGGGCTGTGTATTCGTAAAGAATAGACGTTTCATTTTGATATTTCCAACGGACTTGGAA
ATATAGATTGAAGTAAAGTT

Downstream 100 bases:

>100_bases
TTCAAATCTACCTATATAAAAAAGGAAGCTTTGGCTTCCTTTTTTATTGGCTGACTGAAAAGTACAGTTTAAATGAGCAG
CTAAGCCATGACAGTTGTGT

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MAKKPIYKSLYFQVIIAIIAGVLVGHFSPSTTQIVNGVEQHIPGLGEQLKPLGDAFIRLIKMIIAPVIFCTVVSGIAGME
SMKSVGKTGGVALLYFEIVSTIALLIGLVVINIAKPGVGMNVDPATLDTSGIQKYVTSGESQSSIDFLMHIIPDTVVGAF
ANGEILQVLLFAILFGFALHKLGDAGRPVLKLIDQIAHVFFNIVNMIMKLAPIGAFGAMAFTIGKYGVGSLVQLGQLIIC
FYITCLLFIFLILGTISRISGFSILKMIRLIREELLIVLGTSSSESVLPRMLRKLEIAGCEKSVVGLVIPTGYSFNLDGT
SIYLTMAAIFIAQATNTQLDIQHQITLLLVLLISSKGAAGVTGSGFIVMAATLSAVGHIPVAGLALILGIDRFMSEARAL
TNLVGNSLATIVVAKWVGALDKDKLNDALNNPDEVDRKMLAPKNPQVAEGLD

Sequences:

>Translated_452_residues
MAKKPIYKSLYFQVIIAIIAGVLVGHFSPSTTQIVNGVEQHIPGLGEQLKPLGDAFIRLIKMIIAPVIFCTVVSGIAGME
SMKSVGKTGGVALLYFEIVSTIALLIGLVVINIAKPGVGMNVDPATLDTSGIQKYVTSGESQSSIDFLMHIIPDTVVGAF
ANGEILQVLLFAILFGFALHKLGDAGRPVLKLIDQIAHVFFNIVNMIMKLAPIGAFGAMAFTIGKYGVGSLVQLGQLIIC
FYITCLLFIFLILGTISRISGFSILKMIRLIREELLIVLGTSSSESVLPRMLRKLEIAGCEKSVVGLVIPTGYSFNLDGT
SIYLTMAAIFIAQATNTQLDIQHQITLLLVLLISSKGAAGVTGSGFIVMAATLSAVGHIPVAGLALILGIDRFMSEARAL
TNLVGNSLATIVVAKWVGALDKDKLNDALNNPDEVDRKMLAPKNPQVAEGLD
>Mature_451_residues
AKKPIYKSLYFQVIIAIIAGVLVGHFSPSTTQIVNGVEQHIPGLGEQLKPLGDAFIRLIKMIIAPVIFCTVVSGIAGMES
MKSVGKTGGVALLYFEIVSTIALLIGLVVINIAKPGVGMNVDPATLDTSGIQKYVTSGESQSSIDFLMHIIPDTVVGAFA
NGEILQVLLFAILFGFALHKLGDAGRPVLKLIDQIAHVFFNIVNMIMKLAPIGAFGAMAFTIGKYGVGSLVQLGQLIICF
YITCLLFIFLILGTISRISGFSILKMIRLIREELLIVLGTSSSESVLPRMLRKLEIAGCEKSVVGLVIPTGYSFNLDGTS
IYLTMAAIFIAQATNTQLDIQHQITLLLVLLISSKGAAGVTGSGFIVMAATLSAVGHIPVAGLALILGIDRFMSEARALT
NLVGNSLATIVVAKWVGALDKDKLNDALNNPDEVDRKMLAPKNPQVAEGLD

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI21314632, Length=408, Percent_Identity=27.9411764705882, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI169790839, Length=423, Percent_Identity=26.9503546099291, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI40254478, Length=417, Percent_Identity=26.1390887290168, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI194239697, Length=433, Percent_Identity=24.7113163972286, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI5032093, Length=417, Percent_Identity=27.3381294964029, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI66773030, Length=414, Percent_Identity=26.0869565217391, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI223468566, Length=288, Percent_Identity=30.9027777777778, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI223468564, Length=289, Percent_Identity=30.7958477508651, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI4827012, Length=372, Percent_Identity=26.6129032258064, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI262359914, Length=377, Percent_Identity=25.1989389920424, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI301601644, Length=150, Percent_Identity=30, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1789947, Length=433, Percent_Identity=71.5935334872979, Blast_Score=629, Evalue=0.0,
Organism=Escherichia coli, GI1790514, Length=442, Percent_Identity=39.1402714932127, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1788024, Length=397, Percent_Identity=25.4408060453401, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789473, Length=327, Percent_Identity=21.1009174311927, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17537407, Length=394, Percent_Identity=26.6497461928934, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI71983106, Length=434, Percent_Identity=25.3456221198157, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71983099, Length=434, Percent_Identity=25.3456221198157, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71996953, Length=436, Percent_Identity=23.394495412844, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI193206654, Length=443, Percent_Identity=23.0248306997743, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI193206505, Length=421, Percent_Identity=23.9904988123515, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17541374, Length=436, Percent_Identity=23.6238532110092, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24583025, Length=438, Percent_Identity=23.972602739726, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI17137668, Length=438, Percent_Identity=23.972602739726, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24583023, Length=438, Percent_Identity=23.972602739726, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI281360483, Length=458, Percent_Identity=23.7991266375546, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17137666, Length=458, Percent_Identity=23.7991266375546, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281360481, Length=458, Percent_Identity=23.7991266375546, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 48036; Mature: 47905

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AKKPIYKSLYFQVIIAIIAGVLVGHFSPSTTQIVNGVEQHIPGLGEQLKPLGDAFIRLI
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
KMIIAPVIFCTVVSGIAGMESMKSVGKTGGVALLYFEIVSTIALLIGLVVINIAKPGVGM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NVDPATLDTSGIQKYVTSGESQSSIDFLMHIIPDTVVGAFANGEILQVLLFAILFGFALH
CCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
KLGDAGRPVLKLIDQIAHVFFNIVNMIMKLAPIGAFGAMAFTIGKYGVGSLVQLGQLIIC
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
FYITCLLFIFLILGTISRISGFSILKMIRLIREELLIVLGTSSSESVLPRMLRKLEIAGC
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCC
EKSVVGLVIPTGYSFNLDGTSIYLTMAAIFIAQATNTQLDIQHQITLLLVLLISSKGAAG
CCCCEEEEEECCEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
VTGSGFIVMAATLSAVGHIPVAGLALILGIDRFMSEARALTNLVGNSLATIVVAKWVGAL
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DKDKLNDALNNPDEVDRKMLAPKNPQVAEGLD
CHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA