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The map label for this gene is prfA [H]

Identifier: 213158536

GI number: 213158536

Start: 2582001

End: 2583089

Strand: Reverse

Name: prfA [H]

Synonym: AB57_2488

Alternate gene names: 213158536

Gene position: 2583089-2582001 (Counterclockwise)

Preceding gene: 213158537

Following gene: 213158535

Centisome position: 63.77

GC content: 43.07

Gene sequence:

>1089_bases
ATGAAAGCGTCACTCCGTTTACGTCTTGATCAACTCTGTGATCGTCATGAAGAACTTACTGCATTGCTTGCAGATGCAGA
AGTGATTTCCGATAACAAACGTTTCCGTAAACTGTCTCGTGAACATAGTGATTTAACTGAAATTACCGAAGTTTGGGGCA
AATACCGTCAAGCAGAAGAAGATATTGAAACTGCTGAAATGATGAAGTCGGACCCTGACTTTAAAGATATGGCAGAAGAA
GAAATTCAAGCAAATAAAGTATTGCTTGAAGAATTAGAAAGCCAGCTTAATATTTTGATGATTCCAAAAGATCCAAATGA
CTCTAATGCTGCATATTTAGAAATTCGTGCCGGGACGGGCGGTGATGAAGCTGCAATTTTCTCAGGCGATTTATTCCGTA
TGTATAGCAAATATGCCGAATCGCAAGGTTGGCGTATTGAAGTCCTTTCTGAAAATGAAGGCGAGCATGGTGGTTTTAAA
GAAGTCATTTGCCGTGTAGATGGTGATGGTGTGTATGGCCGCTTAAAGTTCGAAAGTGGCGCGCATCGTGTACAACGTGT
TCCAGCAACTGAATCACAAGGTCGTGTGCATACGTCAGCTTGTACAGTAGCAATCTTGCCTGAAATTGACGTTGATACCA
ATGTTGAAATTAATCCGGCAGATTTACGTATCGATACTTACCGTGCATCAGGTGCGGGTGGTCAGCACATTAACAAAACC
GATTCAGCGGTGCGTATTACTCATATTCCAACCGGTACAGTCGTTGAGTGCCAAGAAGAGCGTTCACAGCATAAAAACAA
AGCTAAGGCGATGGCATTGTTAGTGTCACGTTTAGAAAATGCAAAACGCGCAGCGGCTGATGCAGCAACTTCTGAAATGC
GCCGTGATTTGGTCGGTTCTGGTGACCGCTCTGAACGTATCCGTACTTATAACTACCCGCAAGGACGTATGACGGATCAC
CGTATTAACCTAACTTTATATAAGTTAGATGCCATTATGGAAGGTGATCTAACGGAATTGCTCGATAGTTTACATCGTGA
ATATCAGGCAGATCAGCTTGCGATGCTTGCACAGGAAAATGGCGGCTAA

Upstream 100 bases:

>100_bases
AATCGGCATAAACTGTAAACTATGCGCTATTTTTATGATTTGGCTTATCTTCTTATATTTATCTAGATAAGTTTTTTGGA
CTTTTGAGATATTTCCTCTT

Downstream 100 bases:

>100_bases
TGAATATTGCTCAAGCGCTTGCAATTCGTGGTGAGCCTGACAGTTATGAACGACAGGAAAATGCTTGGTTACTTGAGTAT
TTTCTAAAAATCAATTCACT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MKASLRLRLDQLCDRHEELTALLADAEVISDNKRFRKLSREHSDLTEITEVWGKYRQAEEDIETAEMMKSDPDFKDMAEE
EIQANKVLLEELESQLNILMIPKDPNDSNAAYLEIRAGTGGDEAAIFSGDLFRMYSKYAESQGWRIEVLSENEGEHGGFK
EVICRVDGDGVYGRLKFESGAHRVQRVPATESQGRVHTSACTVAILPEIDVDTNVEINPADLRIDTYRASGAGGQHINKT
DSAVRITHIPTGTVVECQEERSQHKNKAKAMALLVSRLENAKRAAADAATSEMRRDLVGSGDRSERIRTYNYPQGRMTDH
RINLTLYKLDAIMEGDLTELLDSLHREYQADQLAMLAQENGG

Sequences:

>Translated_362_residues
MKASLRLRLDQLCDRHEELTALLADAEVISDNKRFRKLSREHSDLTEITEVWGKYRQAEEDIETAEMMKSDPDFKDMAEE
EIQANKVLLEELESQLNILMIPKDPNDSNAAYLEIRAGTGGDEAAIFSGDLFRMYSKYAESQGWRIEVLSENEGEHGGFK
EVICRVDGDGVYGRLKFESGAHRVQRVPATESQGRVHTSACTVAILPEIDVDTNVEINPADLRIDTYRASGAGGQHINKT
DSAVRITHIPTGTVVECQEERSQHKNKAKAMALLVSRLENAKRAAADAATSEMRRDLVGSGDRSERIRTYNYPQGRMTDH
RINLTLYKLDAIMEGDLTELLDSLHREYQADQLAMLAQENGG
>Mature_362_residues
MKASLRLRLDQLCDRHEELTALLADAEVISDNKRFRKLSREHSDLTEITEVWGKYRQAEEDIETAEMMKSDPDFKDMAEE
EIQANKVLLEELESQLNILMIPKDPNDSNAAYLEIRAGTGGDEAAIFSGDLFRMYSKYAESQGWRIEVLSENEGEHGGFK
EVICRVDGDGVYGRLKFESGAHRVQRVPATESQGRVHTSACTVAILPEIDVDTNVEINPADLRIDTYRASGAGGQHINKT
DSAVRITHIPTGTVVECQEERSQHKNKAKAMALLVSRLENAKRAAADAATSEMRRDLVGSGDRSERIRTYNYPQGRMTDH
RINLTLYKLDAIMEGDLTELLDSLHREYQADQLAMLAQENGG

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=278, Percent_Identity=49.6402877697842, Blast_Score=269, Evalue=2e-72,
Organism=Homo sapiens, GI34577120, Length=335, Percent_Identity=39.7014925373134, Blast_Score=234, Evalue=8e-62,
Organism=Homo sapiens, GI166795305, Length=179, Percent_Identity=49.7206703910614, Blast_Score=186, Evalue=3e-47,
Organism=Escherichia coli, GI1787462, Length=359, Percent_Identity=61.0027855153203, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI2367172, Length=340, Percent_Identity=38.8235294117647, Blast_Score=227, Evalue=7e-61,
Organism=Caenorhabditis elegans, GI17542784, Length=288, Percent_Identity=33.6805555555556, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6321295, Length=317, Percent_Identity=40.3785488958991, Blast_Score=248, Evalue=1e-66,
Organism=Drosophila melanogaster, GI19921226, Length=308, Percent_Identity=40.5844155844156, Blast_Score=215, Evalue=3e-56,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40614; Mature: 40614

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASLRLRLDQLCDRHEELTALLADAEVISDNKRFRKLSREHSDLTEITEVWGKYRQAEE
CCCCCEEHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIETAEMMKSDPDFKDMAEEEIQANKVLLEELESQLNILMIPKDPNDSNAAYLEIRAGTG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCC
GDEAAIFSGDLFRMYSKYAESQGWRIEVLSENEGEHGGFKEVICRVDGDGVYGRLKFESG
CCCCEEECHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHEEEEECCCCEEEEEEECCC
AHRVQRVPATESQGRVHTSACTVAILPEIDVDTNVEINPADLRIDTYRASGAGGQHINKT
CHHHHCCCCCCCCCCEEECEEEEEEECCCCCCCCCEECCCCEEEEEEECCCCCCCCCCCC
DSAVRITHIPTGTVVECQEERSQHKNKAKAMALLVSRLENAKRAAADAATSEMRRDLVGS
CCEEEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSERIRTYNYPQGRMTDHRINLTLYKLDAIMEGDLTELLDSLHREYQADQLAMLAQEN
CCCCCCCEEECCCCCCCCCEEEEEEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
GG
CC
>Mature Secondary Structure
MKASLRLRLDQLCDRHEELTALLADAEVISDNKRFRKLSREHSDLTEITEVWGKYRQAEE
CCCCCEEHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIETAEMMKSDPDFKDMAEEEIQANKVLLEELESQLNILMIPKDPNDSNAAYLEIRAGTG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCC
GDEAAIFSGDLFRMYSKYAESQGWRIEVLSENEGEHGGFKEVICRVDGDGVYGRLKFESG
CCCCEEECHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHEEEEECCCCEEEEEEECCC
AHRVQRVPATESQGRVHTSACTVAILPEIDVDTNVEINPADLRIDTYRASGAGGQHINKT
CHHHHCCCCCCCCCCEEECEEEEEEECCCCCCCCCEECCCCEEEEEEECCCCCCCCCCCC
DSAVRITHIPTGTVVECQEERSQHKNKAKAMALLVSRLENAKRAAADAATSEMRRDLVGS
CCEEEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSERIRTYNYPQGRMTDHRINLTLYKLDAIMEGDLTELLDSLHREYQADQLAMLAQEN
CCCCCCCEEECCCCCCCCCEEEEEEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
GG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA