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The map label for this gene is hemK [H]

Identifier: 213158535

GI number: 213158535

Start: 2581186

End: 2582001

Strand: Reverse

Name: hemK [H]

Synonym: AB57_2487

Alternate gene names: 213158535

Gene position: 2582001-2581186 (Counterclockwise)

Preceding gene: 213158536

Following gene: 213158531

Centisome position: 63.75

GC content: 42.52

Gene sequence:

>816_bases
ATGAATATTGCTCAAGCGCTTGCAATTCGTGGTGAGCCTGACAGTTATGAACGACAGGAAAATGCTTGGTTACTTGAGTA
TTTTCTAAAAATCAATTCACTCGAATTAAAGTTCAGACTTGAGCAAGAGTTAACAGCCCAACAAGAGCAAGACTACCTGG
CTGGTCTTGAGCGTATCCAAAATGGTGAACCTTTAGCTTACGTGACAGGTTCACAACCATTTTGGACGCTCGATTTAAAA
GTAACGTCAGATACTTTAGTGCCACGACCAGATACTGAAGTTTTGGTCGAAACTGTTTTAAATCTAAATTTGCCAAAGAA
TGCAAATATCGTGGATTTGGGAACGGGGACAGGTGCAATTGCGCTTGCATTGGCAAGTGAACGTCCAGACTGGTTCGTCA
CAGCAACTGATATTTACGCACCTACTTTAGACGTTGCAAAAGAAAATGCACAAACACATGGCTTGCATCATGTGAAATTT
GCTTGTGGCGCTTGGTTTGAAGCACTAGAACCCCAACAATTTGATTTAATTGTTTCTAATCCGCCTTACATTGACCCTGA
AGATGAACACATGCAAGCTTTGGCAACAGAGCCGCGCCGTGCGTTGGTTGCAGACCATCATGGGTTAGCCGATATTGAAA
TTATTATTGCACAAGGCAAAAACTGGTTAAAACCGCAAGGCTGGATTGCACTGGAACATGGTTACGATCAGGGGCAAGCT
GTTCGAGGCATTTTCGCAGAGCATGGTTTTAGTGAGATAAAAAGTATACAAGACTATGGCCGAAATGATCGAGTCACTTT
GGCATGTTGGATTTAA

Upstream 100 bases:

>100_bases
ATGCCATTATGGAAGGTGATCTAACGGAATTGCTCGATAGTTTACATCGTGAATATCAGGCAGATCAGCTTGCGATGCTT
GCACAGGAAAATGGCGGCTA

Downstream 100 bases:

>100_bases
AGTTATTTTTGTAGCGTATCACGCCAACGTAACAACCAGTTTTCTATAAGTTTCATTAAGGTATCGGTTATTTTACCCAA
TAGCGCCAATAAAATAATTG

Product: protein methylase, HemK family

Products: NA

Alternate protein names: M.StyLTHemKP; Protein-(glutamine-N(5)) MTase hemK; Protein-glutamine N-methyltransferase hemK [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MNIAQALAIRGEPDSYERQENAWLLEYFLKINSLELKFRLEQELTAQQEQDYLAGLERIQNGEPLAYVTGSQPFWTLDLK
VTSDTLVPRPDTEVLVETVLNLNLPKNANIVDLGTGTGAIALALASERPDWFVTATDIYAPTLDVAKENAQTHGLHHVKF
ACGAWFEALEPQQFDLIVSNPPYIDPEDEHMQALATEPRRALVADHHGLADIEIIIAQGKNWLKPQGWIALEHGYDQGQA
VRGIFAEHGFSEIKSIQDYGRNDRVTLACWI

Sequences:

>Translated_271_residues
MNIAQALAIRGEPDSYERQENAWLLEYFLKINSLELKFRLEQELTAQQEQDYLAGLERIQNGEPLAYVTGSQPFWTLDLK
VTSDTLVPRPDTEVLVETVLNLNLPKNANIVDLGTGTGAIALALASERPDWFVTATDIYAPTLDVAKENAQTHGLHHVKF
ACGAWFEALEPQQFDLIVSNPPYIDPEDEHMQALATEPRRALVADHHGLADIEIIIAQGKNWLKPQGWIALEHGYDQGQA
VRGIFAEHGFSEIKSIQDYGRNDRVTLACWI
>Mature_271_residues
MNIAQALAIRGEPDSYERQENAWLLEYFLKINSLELKFRLEQELTAQQEQDYLAGLERIQNGEPLAYVTGSQPFWTLDLK
VTSDTLVPRPDTEVLVETVLNLNLPKNANIVDLGTGTGAIALALASERPDWFVTATDIYAPTLDVAKENAQTHGLHHVKF
ACGAWFEALEPQQFDLIVSNPPYIDPEDEHMQALATEPRRALVADHHGLADIEIIIAQGKNWLKPQGWIALEHGYDQGQA
VRGIFAEHGFSEIKSIQDYGRNDRVTLACWI

Specific function: Methylates the translation termination release factor RF1 on 'Gln-235' and RF2 on 'Gln-252' [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=244, Percent_Identity=32.3770491803279, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1787463, Length=230, Percent_Identity=48.2608695652174, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI87082085, Length=185, Percent_Identity=34.5945945945946, Blast_Score=86, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324266, Length=167, Percent_Identity=30.5389221556886, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24582226, Length=231, Percent_Identity=29.4372294372294, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30400; Mature: 30400

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIAQALAIRGEPDSYERQENAWLLEYFLKINSLELKFRLEQELTAQQEQDYLAGLERIQ
CCHHHEEEECCCCCCCHHHCCHHHHHHHHHHCCEEEEEEECHHHHHHHHHHHHHHHHHHC
NGEPLAYVTGSQPFWTLDLKVTSDTLVPRPDTEVLVETVLNLNLPKNANIVDLGTGTGAI
CCCCEEEEECCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCEE
ALALASERPDWFVTATDIYAPTLDVAKENAQTHGLHHVKFACGAWFEALEPQQFDLIVSN
EEEEECCCCCEEEEEECCCCCCHHHHHCCHHHCCEEEEEHHHHHHHHHCCCCEEEEEECC
PPYIDPEDEHMQALATEPRRALVADHHGLADIEIIIAQGKNWLKPQGWIALEHGYDQGQA
CCCCCCCHHHHHHHHCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCCHH
VRGIFAEHGFSEIKSIQDYGRNDRVTLACWI
HHHHHHHCCHHHHHHHHHCCCCCCEEEEEEC
>Mature Secondary Structure
MNIAQALAIRGEPDSYERQENAWLLEYFLKINSLELKFRLEQELTAQQEQDYLAGLERIQ
CCHHHEEEECCCCCCCHHHCCHHHHHHHHHHCCEEEEEEECHHHHHHHHHHHHHHHHHHC
NGEPLAYVTGSQPFWTLDLKVTSDTLVPRPDTEVLVETVLNLNLPKNANIVDLGTGTGAI
CCCCEEEEECCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCEE
ALALASERPDWFVTATDIYAPTLDVAKENAQTHGLHHVKFACGAWFEALEPQQFDLIVSN
EEEEECCCCCEEEEEECCCCCCHHHHHCCHHHCCEEEEEHHHHHHHHHCCCCEEEEEECC
PPYIDPEDEHMQALATEPRRALVADHHGLADIEIIIAQGKNWLKPQGWIALEHGYDQGQA
CCCCCCCHHHHHHHHCCCCCEEEECCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCCHH
VRGIFAEHGFSEIKSIQDYGRNDRVTLACWI
HHHHHHHCCHHHHHHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 2544564 [H]