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The map label for this gene is ispF [H]

Identifier: 213158380

GI number: 213158380

Start: 2412166

End: 2412648

Strand: Reverse

Name: ispF [H]

Synonym: AB57_2327

Alternate gene names: 213158380

Gene position: 2412648-2412166 (Counterclockwise)

Preceding gene: 213158383

Following gene: 213158375

Centisome position: 59.56

GC content: 39.13

Gene sequence:

>483_bases
ATGGTTGCTCAAATTCGTATTGGACAAGGAATGGATGTGCATGCCTTTGAAGAGGGTAACTTCGTAACCTTAGCAGGCGT
TCAAATTCCGCATACGCATGGTTTGAAGGCACATTCTGATGGCGATGTCGTGCTACATGCATTATGTGATGCATTACTAG
GTGCTCTAGCACTTGGTGATATTGGTCAGCATTTTCCGGATACAGATCCAGAATTTAAAGGAGCGGATAGCCGTGTTCTA
CTTAAACATGTTTATCAATTAATATTAGACCGAGGTTATCATTTAAATAATGCTGATATTACGGTAGCTTGTGAGCGTCC
TAAATTGGCCAAATACAATTTGGAAATGCGTCAAAGCATTGCAGATGTTTTAAATGTAGATTTAAATCAAATTAGTATCA
AAGCGACCACAACCGAAAAACTTGGATTTACTGGTCGCCAAGAAGGAATTTTAGCAACAGCGACGGTGTTAATATCTCAT
TAA

Upstream 100 bases:

>100_bases
CGCTTATACTTTCAGTCTACTTTGCTAAGTGTACTATAGCGAAACACGACGGTTGAATACAAGATTTCTTAAGCTTTTAT
AATAGAAGGGAAGAGTTTAT

Downstream 100 bases:

>100_bases
ACTAAATCATTGGTTTGTTGCAAAGACTGTTGAAGTTCTAAGGTAGCCTTACGGCCTTGAAGCTGTAAGGTAATACTATG
AATGAGTCCTGTCCATGTTT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MVAQIRIGQGMDVHAFEEGNFVTLAGVQIPHTHGLKAHSDGDVVLHALCDALLGALALGDIGQHFPDTDPEFKGADSRVL
LKHVYQLILDRGYHLNNADITVACERPKLAKYNLEMRQSIADVLNVDLNQISIKATTTEKLGFTGRQEGILATATVLISH

Sequences:

>Translated_160_residues
MVAQIRIGQGMDVHAFEEGNFVTLAGVQIPHTHGLKAHSDGDVVLHALCDALLGALALGDIGQHFPDTDPEFKGADSRVL
LKHVYQLILDRGYHLNNADITVACERPKLAKYNLEMRQSIADVLNVDLNQISIKATTTEKLGFTGRQEGILATATVLISH
>Mature_160_residues
MVAQIRIGQGMDVHAFEEGNFVTLAGVQIPHTHGLKAHSDGDVVLHALCDALLGALALGDIGQHFPDTDPEFKGADSRVL
LKHVYQLILDRGYHLNNADITVACERPKLAKYNLEMRQSIADVLNVDLNQISIKATTTEKLGFTGRQEGILATATVLISH

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=55.1948051948052, Blast_Score=166, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 17375; Mature: 17375

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAQIRIGQGMDVHAFEEGNFVTLAGVQIPHTHGLKAHSDGDVVLHALCDALLGALALGD
CEEEEECCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IGQHFPDTDPEFKGADSRVLLKHVYQLILDRGYHLNNADITVACERPKLAKYNLEMRQSI
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCCHHHHHHH
ADVLNVDLNQISIKATTTEKLGFTGRQEGILATATVLISH
HHHHCCCCCEEEEEEECCHHCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MVAQIRIGQGMDVHAFEEGNFVTLAGVQIPHTHGLKAHSDGDVVLHALCDALLGALALGD
CEEEEECCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IGQHFPDTDPEFKGADSRVLLKHVYQLILDRGYHLNNADITVACERPKLAKYNLEMRQSI
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCCHHHHHHH
ADVLNVDLNQISIKATTTEKLGFTGRQEGILATATVLISH
HHHHCCCCCEEEEEEECCHHCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA