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The map label for this gene is ntrC [H]

Identifier: 213158377

GI number: 213158377

Start: 2408038

End: 2409489

Strand: Direct

Name: ntrC [H]

Synonym: AB57_2324

Alternate gene names: 213158377

Gene position: 2408038-2409489 (Clockwise)

Preceding gene: 213158376

Following gene: 213158378

Centisome position: 59.45

GC content: 42.29

Gene sequence:

>1452_bases
ATGCGATGGGTACTTGAAAAGACCTTTAAAGAAGAAGGATTTGACGTCACCAATTTTGAAGAAGCTCAAACAGCACTTGA
ACGTTTACATCATGATGCGCCCGATGTCATCTTAACTGACATTCGTATGCCAGGTATTGATGGGTTAACTTTCTTATCTA
AAGTGAAAAACTCTCACCCCGACTTGCCTGTTATCATTATGACAGCGCATTCGGACTTAGAGTCTGCTGTATCAAGCTAT
CAGACTGGTGCATTTGAGTATTTACCAAAACCATTCGATATTGATGAAGCTTTAGCTCTAGTCAATCGAGCGATTTTGCA
TATCAATAAATTGCAGCAGCAAGAAGCGACAAAAACAGCTTCACCACTACAATCAACAGAAATTATTGGCGAATCTCCTG
CTATGCAAGAGGTTTTCCGAGCGATTGGTCGTTTATCTCAGTCGCATATTACCGTTTTAATTAATGGTGAATCAGGCACA
GGTAAAGAACTGGTTGCGCATGCTTTACATAAACATTCTCCACGACGCGCTAAGCCTTTTATTGCTTTAAACATGGCAGC
AATTCCTAAAGATTTAATCGAAACGGAATTATTTGGTCATGAAAAAGGTGCTTTCACGGGTGCAAACACCCAACATCAAG
GCCGCTTTGAACAAGCAAATGGTGGAACTCTCTTCCTTGATGAAATTGGTGACATGCCATTTGAAACCCAGACTCGATTG
TTACGTGTGCTCGCAGATGGTGAGTTTTACCGAGTTGGTGGTCATATACCGGTTAAAGTAGATGTCAGAATTGTCGCGGC
AACCCATCAAGACTTAGAAAAACTGGTAAATGAAGGTCGTTTCCGTGAAGACTTATACCACCGTCTAAATGTTATTCGAA
TTCATATTCCGAAGCTTGCTCATCGCAGTGAAGACATCCCGATGCTGGCTCAACATTTCTTGGCTCGCGCGGGTAAAGAG
CTAGGTGTAAGTCCAAAGATTTTACGCACTGAAACAACGGACTATATGCAACAATTACCATGGCCGGGTAATGTTCGTCA
GCTTGAGAATACCTGCCGTTGGTTAACCGTCATGATTACTGGTCGTGAGGTTTATCCGGAAGATTTACCTTCTGAGCTTA
AACAGGTTCCACTCCAAAAAAGTAGTGAGACTAGTCAACCAGCACCATCTTTTGAACGTATTTCTTTGCACCATTGGGAT
GAGCTACTAAGCCAATGGGCAATTCAAAAACTTAAAAATGGTGAAATGAAGATTTTAGACATTGCTACTCCAATGTTTGA
GCGCACCTTAATTAATGCAGCACTACAGCAAACTCGTGGGCGCAAACGTCATGCAGCCGAGCTGTTAGGTTGGGGTCGTA
ACACCCTAACCAGAAAGTTAAAAGAGCTAGGCATGGATTCTGCTGATGACGATGATGAGGACGAACATAAAGCAACATTG
TCAGAAGCTTAA

Upstream 100 bases:

>100_bases
TGCCAATCAGTTCCTGGAAAAACAATGTTTAGCTTATATTTACCTTGGGAGTCAGACCGTGTCGCGAAATAAAATATGGG
TTATTGATGACGATCGCGCC

Downstream 100 bases:

>100_bases
AACCGAAGCAAAAATCAAAAAGCGGCTAATTTAATTTAGCCGCTTTTCTTTATGACTATTTAAATCGGAATTGACTATCA
AACTAAAATATGAACATACA

Product: nitrogen metabolism transcriptional regulator, NtrC, Fis Family

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MRWVLEKTFKEEGFDVTNFEEAQTALERLHHDAPDVILTDIRMPGIDGLTFLSKVKNSHPDLPVIIMTAHSDLESAVSSY
QTGAFEYLPKPFDIDEALALVNRAILHINKLQQQEATKTASPLQSTEIIGESPAMQEVFRAIGRLSQSHITVLINGESGT
GKELVAHALHKHSPRRAKPFIALNMAAIPKDLIETELFGHEKGAFTGANTQHQGRFEQANGGTLFLDEIGDMPFETQTRL
LRVLADGEFYRVGGHIPVKVDVRIVAATHQDLEKLVNEGRFREDLYHRLNVIRIHIPKLAHRSEDIPMLAQHFLARAGKE
LGVSPKILRTETTDYMQQLPWPGNVRQLENTCRWLTVMITGREVYPEDLPSELKQVPLQKSSETSQPAPSFERISLHHWD
ELLSQWAIQKLKNGEMKILDIATPMFERTLINAALQQTRGRKRHAAELLGWGRNTLTRKLKELGMDSADDDDEDEHKATL
SEA

Sequences:

>Translated_483_residues
MRWVLEKTFKEEGFDVTNFEEAQTALERLHHDAPDVILTDIRMPGIDGLTFLSKVKNSHPDLPVIIMTAHSDLESAVSSY
QTGAFEYLPKPFDIDEALALVNRAILHINKLQQQEATKTASPLQSTEIIGESPAMQEVFRAIGRLSQSHITVLINGESGT
GKELVAHALHKHSPRRAKPFIALNMAAIPKDLIETELFGHEKGAFTGANTQHQGRFEQANGGTLFLDEIGDMPFETQTRL
LRVLADGEFYRVGGHIPVKVDVRIVAATHQDLEKLVNEGRFREDLYHRLNVIRIHIPKLAHRSEDIPMLAQHFLARAGKE
LGVSPKILRTETTDYMQQLPWPGNVRQLENTCRWLTVMITGREVYPEDLPSELKQVPLQKSSETSQPAPSFERISLHHWD
ELLSQWAIQKLKNGEMKILDIATPMFERTLINAALQQTRGRKRHAAELLGWGRNTLTRKLKELGMDSADDDDEDEHKATL
SEA
>Mature_483_residues
MRWVLEKTFKEEGFDVTNFEEAQTALERLHHDAPDVILTDIRMPGIDGLTFLSKVKNSHPDLPVIIMTAHSDLESAVSSY
QTGAFEYLPKPFDIDEALALVNRAILHINKLQQQEATKTASPLQSTEIIGESPAMQEVFRAIGRLSQSHITVLINGESGT
GKELVAHALHKHSPRRAKPFIALNMAAIPKDLIETELFGHEKGAFTGANTQHQGRFEQANGGTLFLDEIGDMPFETQTRL
LRVLADGEFYRVGGHIPVKVDVRIVAATHQDLEKLVNEGRFREDLYHRLNVIRIHIPKLAHRSEDIPMLAQHFLARAGKE
LGVSPKILRTETTDYMQQLPWPGNVRQLENTCRWLTVMITGREVYPEDLPSELKQVPLQKSSETSQPAPSFERISLHHWD
ELLSQWAIQKLKNGEMKILDIATPMFERTLINAALQQTRGRKRHAAELLGWGRNTLTRKLKELGMDSADDDDEDEHKATL
SEA

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=466, Percent_Identity=64.8068669527897, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI1788550, Length=479, Percent_Identity=41.3361169102296, Blast_Score=347, Evalue=9e-97,
Organism=Escherichia coli, GI1790437, Length=459, Percent_Identity=40.7407407407407, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI1788905, Length=461, Percent_Identity=37.527114967462, Blast_Score=298, Evalue=6e-82,
Organism=Escherichia coli, GI87082117, Length=373, Percent_Identity=38.6058981233244, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1789087, Length=342, Percent_Identity=40.9356725146199, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1789233, Length=341, Percent_Identity=39.2961876832845, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI87082152, Length=305, Percent_Identity=40.655737704918, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1787583, Length=373, Percent_Identity=34.8525469168901, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1786524, Length=350, Percent_Identity=39.1428571428571, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI87081872, Length=326, Percent_Identity=38.9570552147239, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1789828, Length=225, Percent_Identity=35.1111111111111, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI87081858, Length=328, Percent_Identity=30.7926829268293, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789809, Length=172, Percent_Identity=26.7441860465116, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790860, Length=103, Percent_Identity=31.0679611650485, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786599, Length=132, Percent_Identity=28.030303030303, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54525; Mature: 54525

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWVLEKTFKEEGFDVTNFEEAQTALERLHHDAPDVILTDIRMPGIDGLTFLSKVKNSHP
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCC
DLPVIIMTAHSDLESAVSSYQTGAFEYLPKPFDIDEALALVNRAILHINKLQQQEATKTA
CCCEEEEECCCHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SPLQSTEIIGESPAMQEVFRAIGRLSQSHITVLINGESGTGKELVAHALHKHSPRRAKPF
CCCHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCE
IALNMAAIPKDLIETELFGHEKGAFTGANTQHQGRFEQANGGTLFLDEIGDMPFETQTRL
EEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCCCHHHHHH
LRVLADGEFYRVGGHIPVKVDVRIVAATHQDLEKLVNEGRFREDLYHRLNVIRIHIPKLA
HHHHHCCCEEEECCEEEEEEEEEEEEHHHHHHHHHHHCCCHHHHHHHHHCEEEEECCHHH
HRSEDIPMLAQHFLARAGKELGVSPKILRTETTDYMQQLPWPGNVRQLENTCRWLTVMIT
CCCCCCHHHHHHHHHHCCHHCCCCCCEEECCCHHHHHHCCCCCCHHHHHHHHEEEEEEEE
GREVYPEDLPSELKQVPLQKSSETSQPAPSFERISLHHWDELLSQWAIQKLKNGEMKILD
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
IATPMFERTLINAALQQTRGRKRHAAELLGWGRNTLTRKLKELGMDSADDDDEDEHKATL
ECCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHH
SEA
CCC
>Mature Secondary Structure
MRWVLEKTFKEEGFDVTNFEEAQTALERLHHDAPDVILTDIRMPGIDGLTFLSKVKNSHP
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCC
DLPVIIMTAHSDLESAVSSYQTGAFEYLPKPFDIDEALALVNRAILHINKLQQQEATKTA
CCCEEEEECCCHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SPLQSTEIIGESPAMQEVFRAIGRLSQSHITVLINGESGTGKELVAHALHKHSPRRAKPF
CCCHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCE
IALNMAAIPKDLIETELFGHEKGAFTGANTQHQGRFEQANGGTLFLDEIGDMPFETQTRL
EEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCCCHHHHHH
LRVLADGEFYRVGGHIPVKVDVRIVAATHQDLEKLVNEGRFREDLYHRLNVIRIHIPKLA
HHHHHCCCEEEECCEEEEEEEEEEEEHHHHHHHHHHHCCCHHHHHHHHHCEEEEECCHHH
HRSEDIPMLAQHFLARAGKELGVSPKILRTETTDYMQQLPWPGNVRQLENTCRWLTVMIT
CCCCCCHHHHHHHHHHCCHHCCCCCCEEECCCHHHHHHCCCCCCHHHHHHHHEEEEEEEE
GREVYPEDLPSELKQVPLQKSSETSQPAPSFERISLHHWDELLSQWAIQKLKNGEMKILD
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
IATPMFERTLINAALQQTRGRKRHAAELLGWGRNTLTRKLKELGMDSADDDDEDEHKATL
ECCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHH
SEA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]