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The map label for this gene is ntrB [H]

Identifier: 213158376

GI number: 213158376

Start: 2406900

End: 2408009

Strand: Direct

Name: ntrB [H]

Synonym: AB57_2323

Alternate gene names: 213158376

Gene position: 2406900-2408009 (Clockwise)

Preceding gene: 213158363

Following gene: 213158377

Centisome position: 59.42

GC content: 38.92

Gene sequence:

>1110_bases
ATGGATCAACACACACCTATCGACTATCGCCTATTGGTAGACAATTTAACTACTGCGATTTTATTGATCGATAGTAACTT
TAATATTTTTTATTTGAATTCCGCTTGTGAAGCACTTTTTGACATTAGTTTGCTTCGCGCTTCTGGACAACCGGTTTTAA
ACTTACTTCATGCACCTGATGATACCTTTAATACACATGAAGCCTTATTGAACACATTAAAAACGGGACAACCTTATACA
CGTCGTGAAGCCATCATTAATGTCAATTTTAAAGATTTACATGTGGATTATACGGTCTCGCAATTAAATGCAGGTAAAAG
CTACCACCCGCTCTTACTTATTGAGTTAAATCCTATTGATCGTATGTTGAAAATTTCTAAGGAAGAAAACTTAATTCAAC
AACATCAAGTGGCTAGACAACTGGTTCGAGGCGTTGCTCACGAAATTAAAAACCCTCTGGCTGGTATTCGGGGGGCAACT
CAGTTACTTGCTCGTAGTTTAAATGACGAGAGCTATGCTGAATTTACAGATATCATTATTAACGAGGTAGATCGTCTCAC
CAATTTGGCCGACACCATGTTGGGCTCAAGACAACTGCCAAGTTATGAAAATGTAAATGTGCATGAACCTCTAGAACGGG
TACGCTCTTTAATCGCAAATCAGACCAAGAAAAAAATCAAAATTACGCGTGATTATGATTTGTCTTTACCCGATGTAAAA
GCCGATCGCGACCAACTCATTCAAGTCATGCTCAATATTAGCGTTAATGCCATTCAAGCTATTACAGAAAATAAATCATT
TTTTACAGATCAAGAGCCTGAATTAATTTTACGTACCCGTATTCAACGTCTAGTCACCATTAATGGAGTACTCAACCGCT
CTGCTGTACGAGTCGATATTGAAGACAATGGACCGGGTATTCCCGAAAGCATTTTAGAGTCTGTGTTTTACCCTTTGGTT
ACAGGACGAGCAAAAGGCACAGGGCTTGGCCTGAGTATTGCACAGAACATCATGCATCAACATAACGGAATGATTGAATG
CCAATCAGTTCCTGGAAAAACAATGTTTAGCTTATATTTACCTTGGGAGTCAGACCGTGTCGCGAAATAA

Upstream 100 bases:

>100_bases
AAGTTGGCGTACATTTTGCATCGATCATGTTCTATAACGGTGAAAACCGATAACCAATGAAGATGCCATTGCGCCATTTT
AAGACAGGAACGATTCGCTA

Downstream 100 bases:

>100_bases
AATATGGGTTATTGATGACGATCGCGCCATGCGATGGGTACTTGAAAAGACCTTTAAAGAAGAAGGATTTGACGTCACCA
ATTTTGAAGAAGCTCAAACA

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MDQHTPIDYRLLVDNLTTAILLIDSNFNIFYLNSACEALFDISLLRASGQPVLNLLHAPDDTFNTHEALLNTLKTGQPYT
RREAIINVNFKDLHVDYTVSQLNAGKSYHPLLLIELNPIDRMLKISKEENLIQQHQVARQLVRGVAHEIKNPLAGIRGAT
QLLARSLNDESYAEFTDIIINEVDRLTNLADTMLGSRQLPSYENVNVHEPLERVRSLIANQTKKKIKITRDYDLSLPDVK
ADRDQLIQVMLNISVNAIQAITENKSFFTDQEPELILRTRIQRLVTINGVLNRSAVRVDIEDNGPGIPESILESVFYPLV
TGRAKGTGLGLSIAQNIMHQHNGMIECQSVPGKTMFSLYLPWESDRVAK

Sequences:

>Translated_369_residues
MDQHTPIDYRLLVDNLTTAILLIDSNFNIFYLNSACEALFDISLLRASGQPVLNLLHAPDDTFNTHEALLNTLKTGQPYT
RREAIINVNFKDLHVDYTVSQLNAGKSYHPLLLIELNPIDRMLKISKEENLIQQHQVARQLVRGVAHEIKNPLAGIRGAT
QLLARSLNDESYAEFTDIIINEVDRLTNLADTMLGSRQLPSYENVNVHEPLERVRSLIANQTKKKIKITRDYDLSLPDVK
ADRDQLIQVMLNISVNAIQAITENKSFFTDQEPELILRTRIQRLVTINGVLNRSAVRVDIEDNGPGIPESILESVFYPLV
TGRAKGTGLGLSIAQNIMHQHNGMIECQSVPGKTMFSLYLPWESDRVAK
>Mature_369_residues
MDQHTPIDYRLLVDNLTTAILLIDSNFNIFYLNSACEALFDISLLRASGQPVLNLLHAPDDTFNTHEALLNTLKTGQPYT
RREAIINVNFKDLHVDYTVSQLNAGKSYHPLLLIELNPIDRMLKISKEENLIQQHQVARQLVRGVAHEIKNPLAGIRGAT
QLLARSLNDESYAEFTDIIINEVDRLTNLADTMLGSRQLPSYENVNVHEPLERVRSLIANQTKKKIKITRDYDLSLPDVK
ADRDQLIQVMLNISVNAIQAITENKSFFTDQEPELILRTRIQRLVTINGVLNRSAVRVDIEDNGPGIPESILESVFYPLV
TGRAKGTGLGLSIAQNIMHQHNGMIECQSVPGKTMFSLYLPWESDRVAK

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=363, Percent_Identity=41.0468319559229, Blast_Score=256, Evalue=1e-69,
Organism=Escherichia coli, GI1788549, Length=357, Percent_Identity=27.4509803921569, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=29.9559471365639, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790861, Length=232, Percent_Identity=28.8793103448276, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41611; Mature: 41611

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQHTPIDYRLLVDNLTTAILLIDSNFNIFYLNSACEALFDISLLRASGQPVLNLLHAPD
CCCCCCCCCEEECCCCCEEEEEEECCCCEEEECHHHHHHHHHHHHHCCCCHHHHHHCCCC
DTFNTHEALLNTLKTGQPYTRREAIINVNFKDLHVDYTVSQLNAGKSYHPLLLIELNPID
CCCCHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEEEEHHHHCCCCCCCCEEEEEECCHH
RMLKISKEENLIQQHQVARQLVRGVAHEIKNPLAGIRGATQLLARSLNDESYAEFTDIII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NEVDRLTNLADTMLGSRQLPSYENVNVHEPLERVRSLIANQTKKKIKITRDYDLSLPDVK
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHEEEEEECCCCCCCCCC
ADRDQLIQVMLNISVNAIQAITENKSFFTDQEPELILRTRIQRLVTINGVLNRSAVRVDI
CCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
EDNGPGIPESILESVFYPLVTGRAKGTGLGLSIAQNIMHQHNGMIECQSVPGKTMFSLYL
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEEEE
PWESDRVAK
CCCCCCCCC
>Mature Secondary Structure
MDQHTPIDYRLLVDNLTTAILLIDSNFNIFYLNSACEALFDISLLRASGQPVLNLLHAPD
CCCCCCCCCEEECCCCCEEEEEEECCCCEEEECHHHHHHHHHHHHHCCCCHHHHHHCCCC
DTFNTHEALLNTLKTGQPYTRREAIINVNFKDLHVDYTVSQLNAGKSYHPLLLIELNPID
CCCCHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEEEEHHHHCCCCCCCCEEEEEECCHH
RMLKISKEENLIQQHQVARQLVRGVAHEIKNPLAGIRGATQLLARSLNDESYAEFTDIII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NEVDRLTNLADTMLGSRQLPSYENVNVHEPLERVRSLIANQTKKKIKITRDYDLSLPDVK
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHEEEEEECCCCCCCCCC
ADRDQLIQVMLNISVNAIQAITENKSFFTDQEPELILRTRIQRLVTINGVLNRSAVRVDI
CCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
EDNGPGIPESILESVFYPLVTGRAKGTGLGLSIAQNIMHQHNGMIECQSVPGKTMFSLYL
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEEEE
PWESDRVAK
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]