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The map label for this gene is rimO [H]

Identifier: 213158375

GI number: 213158375

Start: 2405215

End: 2406558

Strand: Reverse

Name: rimO [H]

Synonym: AB57_2321

Alternate gene names: 213158375

Gene position: 2406558-2405215 (Counterclockwise)

Preceding gene: 213158380

Following gene: 213158374

Centisome position: 59.41

GC content: 40.48

Gene sequence:

>1344_bases
ATGAAGACCCCCAAAGTCGGTTTTGTTTCTTTAGGTTGTCCTAAGGCATTGGTAGATTCTGAACGAATTTTAACTCAGTT
AAAGACTGACGGTTATCAAGTTGCATCAGATTATGATGGTGCTGATTTAGTTGTTGTTAATACCTGTGGTTTTATTGAAT
CTGCAGTACAAGAATCTTTAGATGCAATTGGCGAAGCCATGAGTGAAAATGGCCGAGTTATTGTGACAGGCTGTTTAGGT
AAAGACGAAGATAAAATTCGTCAAATGCACCCTAATGTTCTAAAAGTTACTGGTGCAGCAGCTTATCAAGACGTTATGGA
AGCTGTACATGAATATGTCCCAGCACCGCCTAAACATAATCCGTTTATTGACCTTGTTCCTGAACAAGGTATCCGTTTAA
CACCAAAACATTATGCTTATTTAAAAATATCTGAAGGCTGTAACCACCGTTGTACCTTCTGTATTATCCCGAGCATGCGC
GGTGATTTAGTATCACGTCCGGTTGGAAGCGTATTAGAAGAAGCTGCTGCGTTAAAACGTGCAGGTGTAAAAGAAATCTT
GGTCATTTCCCAAGATACCTCAGCTTATGGCGTAGACACCAAATATAAGCTCGATTTCTGGAATGGACAGCCCGTAAAAA
CCAAATTCTTTGACATGTGTGAAGCACTTGGTCAGCTTGGAATTTGGGTTCGCTTACATTATGTATATCCATATCCACAT
GTTGATGCAGTAATCGATTTAATGGCTCAAGGTAAAATCTTGCCATACCTCGATATTCCATTCCAGCATGCAAGCCCACG
CGTTCTTAAATTAATGAAACGTCCGGCGCATAGTGAGAATACATTAGAAAAAATTAAATTATGGCGCGAAAAATGCCCGA
ATCTTGTGATCCGTTCTACTTTCGTGGTTGGCTTTCCAGGCGAAACTGAAGAAGACTTCCAGATTTTGCTCGATTGGTTG
GTTGAAGCTCAACTTGATCGTGTAGGCTGCTTTACCTATTCACCAGTAGAAGGCGCTACGGCAAATGATTTACCAGATCA
TGTGCCAGAAGAAATTAAGCAAGAGCGTTACGAGCGCTTTATGCAAGTGCAGCAACAAATCTCTGCTGCCAAATTACAAA
AACGTATTGGTCAAACGATGACTGTATTGGTCGATAGTTTGGAAGATGAATATCCTGTTGCTGTTGCACGTTCTTATGCC
GATGCTCCGGAAATTGACGGTAATGTCTTTGTAGAAGATATCGATAAGAGCACTATTCAACCAGGCGATATGTTGGAAGT
CGAAATTACCGATGCGGATGAATACGACTTATTTGCAAAGTTAATTAAAATTAAATCGGTCTAA

Upstream 100 bases:

>100_bases
TTGGTTGTATTTTGGTGCATTACACAAAGAGTGGGGTTTCCACCTATGCTTGAGACGAGAAAAGACATACAATACGCGCA
TTCAAGTGGAGCGCAGATTC

Downstream 100 bases:

>100_bases
TCGAATCACGATTCAAAAAGCATTCAGTTTAAGAGGTCTTGGAGCAAAATCATGGCGGAAACAATCAGCCCCCGTTATTC
ACGAATTTTATTAAAATTAT

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKTPKVGFVSLGCPKALVDSERILTQLKTDGYQVASDYDGADLVVVNTCGFIESAVQESLDAIGEAMSENGRVIVTGCLG
KDEDKIRQMHPNVLKVTGAAAYQDVMEAVHEYVPAPPKHNPFIDLVPEQGIRLTPKHYAYLKISEGCNHRCTFCIIPSMR
GDLVSRPVGSVLEEAAALKRAGVKEILVISQDTSAYGVDTKYKLDFWNGQPVKTKFFDMCEALGQLGIWVRLHYVYPYPH
VDAVIDLMAQGKILPYLDIPFQHASPRVLKLMKRPAHSENTLEKIKLWREKCPNLVIRSTFVVGFPGETEEDFQILLDWL
VEAQLDRVGCFTYSPVEGATANDLPDHVPEEIKQERYERFMQVQQQISAAKLQKRIGQTMTVLVDSLEDEYPVAVARSYA
DAPEIDGNVFVEDIDKSTIQPGDMLEVEITDADEYDLFAKLIKIKSV

Sequences:

>Translated_447_residues
MKTPKVGFVSLGCPKALVDSERILTQLKTDGYQVASDYDGADLVVVNTCGFIESAVQESLDAIGEAMSENGRVIVTGCLG
KDEDKIRQMHPNVLKVTGAAAYQDVMEAVHEYVPAPPKHNPFIDLVPEQGIRLTPKHYAYLKISEGCNHRCTFCIIPSMR
GDLVSRPVGSVLEEAAALKRAGVKEILVISQDTSAYGVDTKYKLDFWNGQPVKTKFFDMCEALGQLGIWVRLHYVYPYPH
VDAVIDLMAQGKILPYLDIPFQHASPRVLKLMKRPAHSENTLEKIKLWREKCPNLVIRSTFVVGFPGETEEDFQILLDWL
VEAQLDRVGCFTYSPVEGATANDLPDHVPEEIKQERYERFMQVQQQISAAKLQKRIGQTMTVLVDSLEDEYPVAVARSYA
DAPEIDGNVFVEDIDKSTIQPGDMLEVEITDADEYDLFAKLIKIKSV
>Mature_447_residues
MKTPKVGFVSLGCPKALVDSERILTQLKTDGYQVASDYDGADLVVVNTCGFIESAVQESLDAIGEAMSENGRVIVTGCLG
KDEDKIRQMHPNVLKVTGAAAYQDVMEAVHEYVPAPPKHNPFIDLVPEQGIRLTPKHYAYLKISEGCNHRCTFCIIPSMR
GDLVSRPVGSVLEEAAALKRAGVKEILVISQDTSAYGVDTKYKLDFWNGQPVKTKFFDMCEALGQLGIWVRLHYVYPYPH
VDAVIDLMAQGKILPYLDIPFQHASPRVLKLMKRPAHSENTLEKIKLWREKCPNLVIRSTFVVGFPGETEEDFQILLDWL
VEAQLDRVGCFTYSPVEGATANDLPDHVPEEIKQERYERFMQVQQQISAAKLQKRIGQTMTVLVDSLEDEYPVAVARSYA
DAPEIDGNVFVEDIDKSTIQPGDMLEVEITDADEYDLFAKLIKIKSV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=489, Percent_Identity=27.4028629856851, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI28872782, Length=484, Percent_Identity=27.2727272727273, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI93277076, Length=448, Percent_Identity=25.6696428571429, Blast_Score=136, Evalue=5e-32,
Organism=Escherichia coli, GI1787057, Length=439, Percent_Identity=68.3371298405467, Blast_Score=644, Evalue=0.0,
Organism=Escherichia coli, GI1786882, Length=466, Percent_Identity=27.8969957081545, Blast_Score=149, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17553146, Length=480, Percent_Identity=25.4166666666667, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71996771, Length=212, Percent_Identity=31.1320754716981, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21356207, Length=470, Percent_Identity=26.1702127659574, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI19922432, Length=410, Percent_Identity=25.3658536585366, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49999; Mature: 49999

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPKVGFVSLGCPKALVDSERILTQLKTDGYQVASDYDGADLVVVNTCGFIESAVQESL
CCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCHHHHHHHHHHH
DAIGEAMSENGRVIVTGCLGKDEDKIRQMHPNVLKVTGAAAYQDVMEAVHEYVPAPPKHN
HHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCEEEECCHHHHHHHHHHHHHHCCCCCCCC
PFIDLVPEQGIRLTPKHYAYLKISEGCNHRCTFCIIPSMRGDLVSRPVGSVLEEAAALKR
CEEEECCCCCCEECCCCEEEEEECCCCCCCEEEEEECCCCCHHHHCHHHHHHHHHHHHHH
AGVKEILVISQDTSAYGVDTKYKLDFWNGQPVKTKFFDMCEALGQLGIWVRLHYVYPYPH
CCCEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCC
VDAVIDLMAQGKILPYLDIPFQHASPRVLKLMKRPAHSENTLEKIKLWREKCPNLVIRST
HHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEE
FVVGFPGETEEDFQILLDWLVEAQLDRVGCFTYSPVEGATANDLPDHVPEEIKQERYERF
EEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
MQVQQQISAAKLQKRIGQTMTVLVDSLEDEYPVAVARSYADAPEIDGNVFVEDIDKSTIQ
HHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCC
PGDMLEVEITDADEYDLFAKLIKIKSV
CCCEEEEEECCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTPKVGFVSLGCPKALVDSERILTQLKTDGYQVASDYDGADLVVVNTCGFIESAVQESL
CCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCHHHHHHHHHHH
DAIGEAMSENGRVIVTGCLGKDEDKIRQMHPNVLKVTGAAAYQDVMEAVHEYVPAPPKHN
HHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCEEEECCHHHHHHHHHHHHHHCCCCCCCC
PFIDLVPEQGIRLTPKHYAYLKISEGCNHRCTFCIIPSMRGDLVSRPVGSVLEEAAALKR
CEEEECCCCCCEECCCCEEEEEECCCCCCCEEEEEECCCCCHHHHCHHHHHHHHHHHHHH
AGVKEILVISQDTSAYGVDTKYKLDFWNGQPVKTKFFDMCEALGQLGIWVRLHYVYPYPH
CCCEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCC
VDAVIDLMAQGKILPYLDIPFQHASPRVLKLMKRPAHSENTLEKIKLWREKCPNLVIRST
HHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEE
FVVGFPGETEEDFQILLDWLVEAQLDRVGCFTYSPVEGATANDLPDHVPEEIKQERYERF
EEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
MQVQQQISAAKLQKRIGQTMTVLVDSLEDEYPVAVARSYADAPEIDGNVFVEDIDKSTIQ
HHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCC
PGDMLEVEITDADEYDLFAKLIKIKSV
CCCEEEEEECCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA