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The map label for this gene is greB [H]

Identifier: 213158338

GI number: 213158338

Start: 3163093

End: 3163587

Strand: Reverse

Name: greB [H]

Synonym: AB57_3068

Alternate gene names: 213158338

Gene position: 3163587-3163093 (Counterclockwise)

Preceding gene: 213158340

Following gene: 213158336

Centisome position: 78.1

GC content: 38.18

Gene sequence:

>495_bases
ATGAAATCTAACTTAATCACTCGGTCAGGCCATGACAAATTAGTTGCTGAATTAAAACAACTTTGGCATGAAGAAAGACC
AGAAATTACGAAAAAAGTAAATTGGGCTGCAAGCCTCGGTGACCGTAGTGAGAATGCAGATTATCAATATAACAAGCAGC
TCCTTAGAAAAATTGATCGACGAGTACGCTACTTAGGAAAGCGTTTAGAAGAGTTAAAAATTGTTGATTACTCACCTGAG
CAAGAAGGCAAAGTTTACTTTGGTGCTTGGGTCGACATTGAAAATGAAGAAGGGGAGCAAAAAACTTTACGAATTGTCGG
GGTAGATGAAATTTATGATCACCATCCTCAGCATATTTCAATTGAGTCACCTATGGCACGTGCACTCTTGAGTAAAGAAG
TCGATGATGAAGTAGAAGTACACACGCCTTTGGGCAAAAAGCTGTGGTATATCAATGCAATTCGCTATGAAAAACCTAAA
AATGATGAAATTTAG

Upstream 100 bases:

>100_bases
TTTATTAAGGAAGAAAATTTATACACTTCTGATGTGAGAAAATAGGGTATAAAATGCTACATCTTGTAACCCTTATGTTG
TTAATCAGTGGTTTTTAATC

Downstream 100 bases:

>100_bases
ATAGATTTGTTTTTATTTTGAGCGGTTGAATTAAAAATTAAATAAAAGATTTGCCAACTTGCATTTTTGTTTATATTATA
GCGCTCGTTGGAAGCGTGGC

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MKSNLITRSGHDKLVAELKQLWHEERPEITKKVNWAASLGDRSENADYQYNKQLLRKIDRRVRYLGKRLEELKIVDYSPE
QEGKVYFGAWVDIENEEGEQKTLRIVGVDEIYDHHPQHISIESPMARALLSKEVDDEVEVHTPLGKKLWYINAIRYEKPK
NDEI

Sequences:

>Translated_164_residues
MKSNLITRSGHDKLVAELKQLWHEERPEITKKVNWAASLGDRSENADYQYNKQLLRKIDRRVRYLGKRLEELKIVDYSPE
QEGKVYFGAWVDIENEEGEQKTLRIVGVDEIYDHHPQHISIESPMARALLSKEVDDEVEVHTPLGKKLWYINAIRYEKPK
NDEI
>Mature_164_residues
MKSNLITRSGHDKLVAELKQLWHEERPEITKKVNWAASLGDRSENADYQYNKQLLRKIDRRVRYLGKRLEELKIVDYSPE
QEGKVYFGAWVDIENEEGEQKTLRIVGVDEIYDHHPQHISIESPMARALLSKEVDDEVEVHTPLGKKLWYINAIRYEKPK
NDEI

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=159, Percent_Identity=67.9245283018868, Blast_Score=217, Evalue=3e-58,
Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=34.1772151898734, Blast_Score=79, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 19340; Mature: 19340

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSNLITRSGHDKLVAELKQLWHEERPEITKKVNWAASLGDRSENADYQYNKQLLRKIDR
CCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
RVRYLGKRLEELKIVDYSPEQEGKVYFGAWVDIENEEGEQKTLRIVGVDEIYDHHPQHIS
HHHHHHHHHHHCEEECCCCCCCCCEEEEEEEEECCCCCCCEEEEEEEHHHHHHCCCCEEE
IESPMARALLSKEVDDEVEVHTPLGKKLWYINAIRYEKPKNDEI
CCCHHHHHHHHHCCCCCEEEECCCCCEEEEEEEEECCCCCCCCC
>Mature Secondary Structure
MKSNLITRSGHDKLVAELKQLWHEERPEITKKVNWAASLGDRSENADYQYNKQLLRKIDR
CCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
RVRYLGKRLEELKIVDYSPEQEGKVYFGAWVDIENEEGEQKTLRIVGVDEIYDHHPQHIS
HHHHHHHHHHHCEEECCCCCCCCCEEEEEEEEECCCCCCCEEEEEEEHHHHHHCCCCEEE
IESPMARALLSKEVDDEVEVHTPLGKKLWYINAIRYEKPKNDEI
CCCHHHHHHHHHCCCCCEEEECCCCCEEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]