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The map label for this gene is pdxB

Identifier: 213158323

GI number: 213158323

Start: 3145592

End: 3146659

Strand: Reverse

Name: pdxB

Synonym: AB57_3051

Alternate gene names: 213158323

Gene position: 3146659-3145592 (Counterclockwise)

Preceding gene: 213158324

Following gene: 213158322

Centisome position: 77.69

GC content: 41.85

Gene sequence:

>1068_bases
ATGAAAATCGTCGCAGATGAAAATTTGGCGTTTACCGATTACTTTTTTTCAGAGTTTGGCGATATTCAACATAAAGCAGG
GCGCACCTTAACGCATACAGACGTACAAGATGCTGAAGCTTTATTGGTCCGTTCAGTCACCGCAGTAAATGAAAGTTTAA
TTCAAAATACCGCCCTAAAATATGTGGGTAGTGCCACAATCGGGACCGATCATTTAGATATTCAAGCTTTAGAAAAGCAT
GGCATTACATGGGCCAATGCCGCAGGTTGTAATGCACAGGCGGTTGCTGAATATGTGATTACAGCTTTACTGCATTTAGA
TGCTAGCCTTTTAGAACAACAAGAAAAATTTACGCTAGGCATTGTGGGGCTTGGCAATGTCGGTAAGCGCTTGGTCTATA
TGGCTCAGTTGCTTGGTTGGAAAGTGATTGGCTTCGACCCCTATGTACAGTTAGATTCAATTGAAAATGTAAGCTTTCAA
GCCTTGTTGCAACAAGCCAATGCAGTTTCAATTCACGTGCCGCTGACCAAAAAAGGCGAACATGCGACGTATCATCTGTT
TGACGAAAAAGCTTTTGCAGCGCTTCAACCGAACACGATTTTAATTAATAGTGCACGCGGTCCGGTCGTAAAAGAAGCCG
CTTTAATCGAAGATATTCAACGTACCCAGCGTAAAGTTGTACTTGATGTATTTGAGCATGAGCCAGTGATATCTGAAGAG
CTTTTAAATATGTTGGCTTTGGCAACACCGCATATTGCAGGTTATAGCTTGGAAGGAAAAGCTCGTGGTACGCAAATGAT
TTATGAAGCGTTTTGTCAAAAATTTGGTTATGAGATTAATAAGCGTTTCGAAACCCAGTTGCCTGCATGTGAAGACTATT
TTTCAGGACATGATTTAAAAGCAGTGCTCAAGCAAAAACTATCTCAAATTTATGATATTGCTCAAGATGATGCCAACATC
CGTGCCTGTGTTAAAGAGGGCAAGGTAGAGCAAAAAGCATTTGATTTATTACGTAAGAATTATCCGCTGCGCCGTGAATG
GGCAGCTCACGGGGGACCACAGGCATGA

Upstream 100 bases:

>100_bases
ACTGAGTCTTGAAACCCTGTACTAATTTAATACCCTTAACATTGTACTTTGCCACAACTCAAAGGATAATAACGTTTTTA
TAAGCTATGGGTTGCAGACC

Downstream 100 bases:

>100_bases
GTTTAAGTTATCAACCTACTTGTTCAATTGATGCTTTAAAGGCTCGTGCCAAGCTTTATACTCAAATCCGTCAGTTTTTT
GCAGAACGTGGGGTCATGGA

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKIVADENLAFTDYFFSEFGDIQHKAGRTLTHTDVQDAEALLVRSVTAVNESLIQNTALKYVGSATIGTDHLDIQALEKH
GITWANAAGCNAQAVAEYVITALLHLDASLLEQQEKFTLGIVGLGNVGKRLVYMAQLLGWKVIGFDPYVQLDSIENVSFQ
ALLQQANAVSIHVPLTKKGEHATYHLFDEKAFAALQPNTILINSARGPVVKEAALIEDIQRTQRKVVLDVFEHEPVISEE
LLNMLALATPHIAGYSLEGKARGTQMIYEAFCQKFGYEINKRFETQLPACEDYFSGHDLKAVLKQKLSQIYDIAQDDANI
RACVKEGKVEQKAFDLLRKNYPLRREWAAHGGPQA

Sequences:

>Translated_355_residues
MKIVADENLAFTDYFFSEFGDIQHKAGRTLTHTDVQDAEALLVRSVTAVNESLIQNTALKYVGSATIGTDHLDIQALEKH
GITWANAAGCNAQAVAEYVITALLHLDASLLEQQEKFTLGIVGLGNVGKRLVYMAQLLGWKVIGFDPYVQLDSIENVSFQ
ALLQQANAVSIHVPLTKKGEHATYHLFDEKAFAALQPNTILINSARGPVVKEAALIEDIQRTQRKVVLDVFEHEPVISEE
LLNMLALATPHIAGYSLEGKARGTQMIYEAFCQKFGYEINKRFETQLPACEDYFSGHDLKAVLKQKLSQIYDIAQDDANI
RACVKEGKVEQKAFDLLRKNYPLRREWAAHGGPQA
>Mature_355_residues
MKIVADENLAFTDYFFSEFGDIQHKAGRTLTHTDVQDAEALLVRSVTAVNESLIQNTALKYVGSATIGTDHLDIQALEKH
GITWANAAGCNAQAVAEYVITALLHLDASLLEQQEKFTLGIVGLGNVGKRLVYMAQLLGWKVIGFDPYVQLDSIENVSFQ
ALLQQANAVSIHVPLTKKGEHATYHLFDEKAFAALQPNTILINSARGPVVKEAALIEDIQRTQRKVVLDVFEHEPVISEE
LLNMLALATPHIAGYSLEGKARGTQMIYEAFCQKFGYEINKRFETQLPACEDYFSGHDLKAVLKQKLSQIYDIAQDDANI
RACVKEGKVEQKAFDLLRKNYPLRREWAAHGGPQA

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily

Homologues:

Organism=Homo sapiens, GI23308577, Length=265, Percent_Identity=28.6792452830189, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI145580578, Length=255, Percent_Identity=27.843137254902, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI4557499, Length=255, Percent_Identity=27.843137254902, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI145580575, Length=255, Percent_Identity=27.843137254902, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI61743967, Length=253, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI4557497, Length=253, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1788660, Length=360, Percent_Identity=42.2222222222222, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1789279, Length=259, Percent_Identity=31.2741312741313, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787645, Length=204, Percent_Identity=24.0196078431373, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87082289, Length=261, Percent_Identity=27.9693486590038, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17532191, Length=262, Percent_Identity=31.2977099236641, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6324055, Length=289, Percent_Identity=26.643598615917, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322116, Length=264, Percent_Identity=27.2727272727273, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320925, Length=264, Percent_Identity=26.8939393939394, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24646446, Length=253, Percent_Identity=29.6442687747036, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24646448, Length=253, Percent_Identity=29.6442687747036, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24646452, Length=253, Percent_Identity=29.6442687747036, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24646450, Length=253, Percent_Identity=29.6442687747036, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI62472511, Length=253, Percent_Identity=29.6442687747036, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19921140, Length=268, Percent_Identity=23.5074626865672, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXB_ACIB3 (B7GY62)

Other databases:

- EMBL:   CP001172
- RefSeq:   YP_002324752.1
- ProteinModelPortal:   B7GY62
- SMR:   B7GY62
- GeneID:   7059383
- GenomeReviews:   CP001172_GR
- KEGG:   abb:ABBFA_000839
- HOGENOM:   HBG289972
- OMA:   SAPGCNA
- ProtClustDB:   CLSK707643
- GO:   GO:0005737
- HAMAP:   MF_01825
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10996:SF4

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: =1.1.1.290

Molecular weight: Translated: 39426; Mature: 39426

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 206-206 ACT_SITE 234-234 ACT_SITE 251-251 BINDING 45-45 BINDING 66-66 BINDING 146-146 BINDING 229-229 BINDING 254-254 BINDING 255-255

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVADENLAFTDYFFSEFGDIQHKAGRTLTHTDVQDAEALLVRSVTAVNESLIQNTALK
CEEEECCCCCHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YVGSATIGTDHLDIQALEKHGITWANAAGCNAQAVAEYVITALLHLDASLLEQQEKFTLG
HHCCCCCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
IVGLGNVGKRLVYMAQLLGWKVIGFDPYVQLDSIENVSFQALLQQANAVSIHVPLTKKGE
EEECCHHHHHHHHHHHHHCCEEECCCCCEEECCCCCHHHHHHHHHCCEEEEEEECCCCCC
HATYHLFDEKAFAALQPNTILINSARGPVVKEAALIEDIQRTQRKVVLDVFEHEPVISEE
CCEEEEECCCHHHEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LLNMLALATPHIAGYSLEGKARGTQMIYEAFCQKFGYEINKRFETQLPACEDYFSGHDLK
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHCCCCHHH
AVLKQKLSQIYDIAQDDANIRACVKEGKVEQKAFDLLRKNYPLRREWAAHGGPQA
HHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MKIVADENLAFTDYFFSEFGDIQHKAGRTLTHTDVQDAEALLVRSVTAVNESLIQNTALK
CEEEECCCCCHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YVGSATIGTDHLDIQALEKHGITWANAAGCNAQAVAEYVITALLHLDASLLEQQEKFTLG
HHCCCCCCCCCHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
IVGLGNVGKRLVYMAQLLGWKVIGFDPYVQLDSIENVSFQALLQQANAVSIHVPLTKKGE
EEECCHHHHHHHHHHHHHCCEEECCCCCEEECCCCCHHHHHHHHHCCEEEEEEECCCCCC
HATYHLFDEKAFAALQPNTILINSARGPVVKEAALIEDIQRTQRKVVLDVFEHEPVISEE
CCEEEEECCCHHHEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
LLNMLALATPHIAGYSLEGKARGTQMIYEAFCQKFGYEINKRFETQLPACEDYFSGHDLK
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHCCCCHHH
AVLKQKLSQIYDIAQDDANIRACVKEGKVEQKAFDLLRKNYPLRREWAAHGGPQA
HHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA