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The map label for this gene is yjeA [H]

Identifier: 213158322

GI number: 213158322

Start: 3144621

End: 3145595

Strand: Reverse

Name: yjeA [H]

Synonym: AB57_3050

Alternate gene names: 213158322

Gene position: 3145595-3144621 (Counterclockwise)

Preceding gene: 213158323

Following gene: 213158319

Centisome position: 77.66

GC content: 41.95

Gene sequence:

>975_bases
ATGAGTTTAAGTTATCAACCTACTTGTTCAATTGATGCTTTAAAGGCTCGTGCCAAGCTTTATACTCAAATCCGTCAGTT
TTTTGCAGAACGTGGGGTCATGGAAGTTGAAACACCCGTGGTTTCACAAGCAGGTGTGACTGACGTACATTTAGCCTCGG
TTCAGGCTTTACGTCATATCAATGGCAAATTGCAGACTCAGTATTTACAGACTTCTCCTGAATTTGCCATGAAACGTTTA
CTGGCAAGTGGCAGCGGACCGATTTATCAAATCTGTAAGGTTTTCCGAGACGATGAGCACGGGCGTAAACACAACAGTGA
ATTTACCATGCTTGAATGGTACCGTCCGGGTCTGGACTTAAAAGCGTTAATGCACGAAACAGCCGATTTATTACAAACCT
GTTTGCAGCATCGTTTTGGCGAAGTTCGTCCATTTATTTTAAGTTATAAGCATGCTTTTCAGGACCGTTTAGATATTAAC
CCGTTGCAAGCGACTTTGAAGCAACTTAAAGATACAGCAAATCGTGTTGGGTTAAATCTCGACTTGGGCGATGACCGTTT
AGCTTATATGGATTTGTTGTTTTCTCATTTTGTTGAACCAAGTTTAGGGTTTGATGCACCTGTCTTTTTAACTGACTTTC
CACCAGAAATGGCATCTTTAGCAAAAGTGAAATTAGATGAAGATGGGGAAGAGGTTGCAGCGCGTTTTGAGGTTTATATC
GAAGGCTTAGAGCTAGCCAATGCTTACGATGAATTGCTCGATTCAGACGTGTTGCGTTCTCGCTTTGAAGCAGATAATGC
AGAGCGAGCTAAACAAGGACTTCATATCATGCCATTAGATGAGCACTTGTTGGCTGCGTTACCTCATATGCCTGAATGTT
CAGGGATCGCTTTGGGAATTGATCGATTACTCATGGTTGCAACCGATCAACTTAAAATTGAAAAAGTCATTGCTTTTCCA
GCCGAAATTGCCTGA

Upstream 100 bases:

>100_bases
CCTGTGTTAAAGAGGGCAAGGTAGAGCAAAAAGCATTTGATTTATTACGTAAGAATTATCCGCTGCGCCGTGAATGGGCA
GCTCACGGGGGACCACAGGC

Downstream 100 bases:

>100_bases
AAACAACGAAATAAAAAAGCCACAATAATGTGGCTTTTTTATTTAGAACTTATTTAAGTTAGGCTTGTTGAATACCTGCA
ACAACCCAATCTTGCTGTGA

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSLSYQPTCSIDALKARAKLYTQIRQFFAERGVMEVETPVVSQAGVTDVHLASVQALRHINGKLQTQYLQTSPEFAMKRL
LASGSGPIYQICKVFRDDEHGRKHNSEFTMLEWYRPGLDLKALMHETADLLQTCLQHRFGEVRPFILSYKHAFQDRLDIN
PLQATLKQLKDTANRVGLNLDLGDDRLAYMDLLFSHFVEPSLGFDAPVFLTDFPPEMASLAKVKLDEDGEEVAARFEVYI
EGLELANAYDELLDSDVLRSRFEADNAERAKQGLHIMPLDEHLLAALPHMPECSGIALGIDRLLMVATDQLKIEKVIAFP
AEIA

Sequences:

>Translated_324_residues
MSLSYQPTCSIDALKARAKLYTQIRQFFAERGVMEVETPVVSQAGVTDVHLASVQALRHINGKLQTQYLQTSPEFAMKRL
LASGSGPIYQICKVFRDDEHGRKHNSEFTMLEWYRPGLDLKALMHETADLLQTCLQHRFGEVRPFILSYKHAFQDRLDIN
PLQATLKQLKDTANRVGLNLDLGDDRLAYMDLLFSHFVEPSLGFDAPVFLTDFPPEMASLAKVKLDEDGEEVAARFEVYI
EGLELANAYDELLDSDVLRSRFEADNAERAKQGLHIMPLDEHLLAALPHMPECSGIALGIDRLLMVATDQLKIEKVIAFP
AEIA
>Mature_323_residues
SLSYQPTCSIDALKARAKLYTQIRQFFAERGVMEVETPVVSQAGVTDVHLASVQALRHINGKLQTQYLQTSPEFAMKRLL
ASGSGPIYQICKVFRDDEHGRKHNSEFTMLEWYRPGLDLKALMHETADLLQTCLQHRFGEVRPFILSYKHAFQDRLDINP
LQATLKQLKDTANRVGLNLDLGDDRLAYMDLLFSHFVEPSLGFDAPVFLTDFPPEMASLAKVKLDEDGEEVAARFEVYIE
GLELANAYDELLDSDVLRSRFEADNAERAKQGLHIMPLDEHLLAALPHMPECSGIALGIDRLLMVATDQLKIEKVIAFPA
EIA

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=340, Percent_Identity=27.3529411764706, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI194272210, Length=340, Percent_Identity=27.3529411764706, Blast_Score=104, Evalue=8e-23,
Organism=Escherichia coli, GI87082379, Length=328, Percent_Identity=46.3414634146341, Blast_Score=278, Evalue=4e-76,
Organism=Escherichia coli, GI1789256, Length=329, Percent_Identity=30.0911854103343, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1790571, Length=325, Percent_Identity=29.8461538461538, Blast_Score=126, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71994340, Length=343, Percent_Identity=27.4052478134111, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17535927, Length=343, Percent_Identity=27.4052478134111, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17535925, Length=343, Percent_Identity=27.4052478134111, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6324256, Length=351, Percent_Identity=27.6353276353276, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320242, Length=340, Percent_Identity=26.4705882352941, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24640851, Length=349, Percent_Identity=26.647564469914, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24640849, Length=349, Percent_Identity=26.647564469914, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 36517; Mature: 36386

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSYQPTCSIDALKARAKLYTQIRQFFAERGVMEVETPVVSQAGVTDVHLASVQALRHI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHH
NGKLQTQYLQTSPEFAMKRLLASGSGPIYQICKVFRDDEHGRKHNSEFTMLEWYRPGLDL
CCHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHH
KALMHETADLLQTCLQHRFGEVRPFILSYKHAFQDRLDINPLQATLKQLKDTANRVGLNL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEE
DLGDDRLAYMDLLFSHFVEPSLGFDAPVFLTDFPPEMASLAKVKLDEDGEEVAARFEVYI
ECCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHH
EGLELANAYDELLDSDVLRSRFEADNAERAKQGLHIMPLDEHLLAALPHMPECSGIALGI
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEECCHHHHHHHCCCCCCCCCHHHHH
DRLLMVATDQLKIEKVIAFPAEIA
HHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SLSYQPTCSIDALKARAKLYTQIRQFFAERGVMEVETPVVSQAGVTDVHLASVQALRHI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHH
NGKLQTQYLQTSPEFAMKRLLASGSGPIYQICKVFRDDEHGRKHNSEFTMLEWYRPGLDL
CCHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHH
KALMHETADLLQTCLQHRFGEVRPFILSYKHAFQDRLDINPLQATLKQLKDTANRVGLNL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEE
DLGDDRLAYMDLLFSHFVEPSLGFDAPVFLTDFPPEMASLAKVKLDEDGEEVAARFEVYI
ECCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHH
EGLELANAYDELLDSDVLRSRFEADNAERAKQGLHIMPLDEHLLAALPHMPECSGIALGI
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEECCHHHHHHHCCCCCCCCCHHHHH
DRLLMVATDQLKIEKVIAFPAEIA
HHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA