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The map label for this gene is dapF

Identifier: 213158316

GI number: 213158316

Start: 3137730

End: 3138575

Strand: Reverse

Name: dapF

Synonym: AB57_3043

Alternate gene names: 213158316

Gene position: 3138575-3137730 (Counterclockwise)

Preceding gene: 213158317

Following gene: 213158315

Centisome position: 77.49

GC content: 42.91

Gene sequence:

>846_bases
ATGTTATTAGAATTTACCAAAATGCATGGTTTAGGCAATGATTTCATGGTGGTTGATCTAATTAGCCAAAGAGCTTATTT
AGATACTGCAACAATTCAACGTTTGGCAGACCGACACTTTGGTGTGGGGTTTGATCAGCTTTTAATTGTTGAACCACCAG
ATGTACCCGAAGCTGATTTTAAATACCGTATTTTCAATGCAGATGGTTCTGAGGTTGAGCAATGTGGTAATGGTGTGCGT
TGTTTTGCCCGTTTTGTCCACGAACGTCATTTAACCAATAAAACCAACATTACCGTACAAACCAAAGCGGGTATTGTAAA
GCCAGAGCTTGGGCAAAATGGTTGGGTGCGCGTAAATATGGGCTACCCAAAGTTTCTACCAAATGAAATTCCATTTGTTG
CCGAAGAGCCAGAAGCTTTATATACCCTTGAGTTGGCAAATGACCAAAACATTAGTATTGATGTGGTCAACATGGGTAAC
CCACACGCGGTCACTATAGTTCCAGATGTACTAACTGCTGATGTAGCTGGTATTGGTCCGCAAGTTGAATCACATAAACG
TTTCCCTGAGCGCGTTAATGCTGGTTTCATGCAAGTGATTGATGACAAGCATGTTCGCTTACGTGTATTTGAACGTGGTG
TGGGTGAAACACTTGCGTGTGGTACAGGAGCTTGTGCTGCTGCGGTAAGTGGCATGCGCCGTGGTCTACTTGCGAACTCT
GTAGAAGTTGAACTTGCTGGTGGTAAGCTACAAATTGAATGGCAAGAAGGCGATGTGGTCTGGATGACTGGTCCAACAAC
TCATGTATATGATGGACGTTTGGATTTACGTTATTTCCAAGGTTAA

Upstream 100 bases:

>100_bases
AAGTCATGGTATCAGGTGAAAAAAGCTATTTAATTCGTGAACGTGAAACGGTTGAGTCGCTTTGGGAAAAAGAACGTTTA
TTGCCTGAGGAGTAAATGAG

Downstream 100 bases:

>100_bases
TCGCATGGCCTAAATAGAAGCCTCTTTTCATCAGTTAAAGTGATAAAAGAGGCTTTCTTTTATATGTACAATAATTTCTT
AATTTCATTACCTTATAAAA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MLLEFTKMHGLGNDFMVVDLISQRAYLDTATIQRLADRHFGVGFDQLLIVEPPDVPEADFKYRIFNADGSEVEQCGNGVR
CFARFVHERHLTNKTNITVQTKAGIVKPELGQNGWVRVNMGYPKFLPNEIPFVAEEPEALYTLELANDQNISIDVVNMGN
PHAVTIVPDVLTADVAGIGPQVESHKRFPERVNAGFMQVIDDKHVRLRVFERGVGETLACGTGACAAAVSGMRRGLLANS
VEVELAGGKLQIEWQEGDVVWMTGPTTHVYDGRLDLRYFQG

Sequences:

>Translated_281_residues
MLLEFTKMHGLGNDFMVVDLISQRAYLDTATIQRLADRHFGVGFDQLLIVEPPDVPEADFKYRIFNADGSEVEQCGNGVR
CFARFVHERHLTNKTNITVQTKAGIVKPELGQNGWVRVNMGYPKFLPNEIPFVAEEPEALYTLELANDQNISIDVVNMGN
PHAVTIVPDVLTADVAGIGPQVESHKRFPERVNAGFMQVIDDKHVRLRVFERGVGETLACGTGACAAAVSGMRRGLLANS
VEVELAGGKLQIEWQEGDVVWMTGPTTHVYDGRLDLRYFQG
>Mature_281_residues
MLLEFTKMHGLGNDFMVVDLISQRAYLDTATIQRLADRHFGVGFDQLLIVEPPDVPEADFKYRIFNADGSEVEQCGNGVR
CFARFVHERHLTNKTNITVQTKAGIVKPELGQNGWVRVNMGYPKFLPNEIPFVAEEPEALYTLELANDQNISIDVVNMGN
PHAVTIVPDVLTADVAGIGPQVESHKRFPERVNAGFMQVIDDKHVRLRVFERGVGETLACGTGACAAAVSGMRRGLLANS
VEVELAGGKLQIEWQEGDVVWMTGPTTHVYDGRLDLRYFQG

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=275, Percent_Identity=58.1818181818182, Blast_Score=322, Evalue=2e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_ACIB3 (B7GY71)

Other databases:

- EMBL:   CP001172
- RefSeq:   YP_002324761.1
- ProteinModelPortal:   B7GY71
- SMR:   B7GY71
- GeneID:   7059148
- GenomeReviews:   CP001172_GR
- KEGG:   abb:ABBFA_000848
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 31140; Mature: 31140

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 220-220

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLEFTKMHGLGNDFMVVDLISQRAYLDTATIQRLADRHFGVGFDQLLIVEPPDVPEADF
CEEEEEHHCCCCCCEEEEEEHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC
KYRIFNADGSEVEQCGNGVRCFARFVHERHLTNKTNITVQTKAGIVKPELGQNGWVRVNM
EEEEECCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEEEEC
GYPKFLPNEIPFVAEEPEALYTLELANDQNISIDVVNMGNPHAVTIVPDVLTADVAGIGP
CCCCCCCCCCCEEECCCCEEEEEEECCCCCEEEEEEECCCCEEEEEECCHHHHHHHCCCC
QVESHKRFPERVNAGFMQVIDDKHVRLRVFERGVGETLACGTGACAAAVSGMRRGLLANS
CCHHHHCCHHHHCCCCEEECCCCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHCCCCCC
VEVELAGGKLQIEWQEGDVVWMTGPTTHVYDGRLDLRYFQG
EEEEEECCEEEEEEECCCEEEEECCCCEEECCEEEEEEECC
>Mature Secondary Structure
MLLEFTKMHGLGNDFMVVDLISQRAYLDTATIQRLADRHFGVGFDQLLIVEPPDVPEADF
CEEEEEHHCCCCCCEEEEEEHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC
KYRIFNADGSEVEQCGNGVRCFARFVHERHLTNKTNITVQTKAGIVKPELGQNGWVRVNM
EEEEECCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCEEEEEC
GYPKFLPNEIPFVAEEPEALYTLELANDQNISIDVVNMGNPHAVTIVPDVLTADVAGIGP
CCCCCCCCCCCEEECCCCEEEEEEECCCCCEEEEEEECCCCEEEEEECCHHHHHHHCCCC
QVESHKRFPERVNAGFMQVIDDKHVRLRVFERGVGETLACGTGACAAAVSGMRRGLLANS
CCHHHHCCHHHHCCCCEEECCCCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHCCCCCC
VEVELAGGKLQIEWQEGDVVWMTGPTTHVYDGRLDLRYFQG
EEEEEECCEEEEEEECCCEEEEECCCCEEECCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA