Click here to switch to the map view.

The map label for this gene is ruvB [H]

Identifier: 213158274

GI number: 213158274

Start: 3089350

End: 3090354

Strand: Direct

Name: ruvB [H]

Synonym: AB57_2995

Alternate gene names: 213158274

Gene position: 3089350-3090354 (Clockwise)

Preceding gene: 213158273

Following gene: 213158275

Centisome position: 76.27

GC content: 45.57

Gene sequence:

>1005_bases
ATGCAAGACCGTCTAATCAGTGGAACAGAAAAACCTGAAGATCATTTTGATCGGGCTATTCGACCGACTTCACTTGCCGA
TTATATCGGGCAACCAGTCGTACGCGAGCAAATGGAAATTTTCATCGGTGCGGCTCGTGGTCGTGGTGAAGCACTCGATC
ACACACTGATTTTCGGTCCACCGGGCTTAGGAAAAACCACGCTTGCCAATATTATTGCGCGTGAAATGGGTGGCAATCTC
AAATCCACTTCAGGGCCTGTTTTAGAGCGTGCGGGTGATTTGGCTGCAATGCTGACTAACCTTGAAGAAGGCGATGTTTT
ATTTATTGATGAAATTCACCGTCTTTCACCAGTGATTGAAGAAATTCTTTATCCGGCAATGGAAGATTACCAACTTGATA
TCATGATTGGTGAAGGTCCTGCTGCTCGTTCAATTAAACTCGATTTACCACCTTTTACGCTCGTTGCTGCGACCACACGT
GCCGGCTTACTCACCTCGCCTCTACGTGACCGTTTCGGTATTGTGCAACGTCTTGAATTCTATTCTGTAGAAGACCTAAC
TCACATCGTGTCACGATCTGCAAACCTCATGGATGTACCCATTACAGTTGAGGGTGCAGAAGAAGTTGCACGCCGTTCAC
GCGGAACACCGCGTATTGCCAATCGTTTATTACGCCGTGTACGTGACTATGCACAAGTAAAAGGTACAGGCGAAGTTAAC
CATGAAATGGCACAACGTGCGCTTGATATGCTTAATGTCGATAAAGCTGGTTTAGATACCTTAGACCGACGTTATTTAAG
TATGTTGTTAGAACGTTTTGATGGTGGACCTGCTGGTGTTGAAGCCCTTGCAGCTGCTATGGCTGAAGATAGCGGAACAC
TTGAAGATGTTATTGAACCTTATTTAATTCAGCAAGGTTATGTTATGCGTACTGCACGAGGTCGCATTGCAACCAACCAA
TCTTATTTGCAGTTTGGCATGACGCCACCTGAGCCTAAAAACTAA

Upstream 100 bases:

>100_bases
TTGAGGCACAAAAAGCTGTTGCGGCGGTTAAAGCCGATTACACTGAATCAGCCGATATTATTCGTGCTGCACTTAAATCA
ATGATGAAGTAAACACACCT

Downstream 100 bases:

>100_bases
TCAAAGGAAGAGAATTTTCTCTTCCTTTTTTTTATTTAACTATTCTTTTTAACTCAAATAAAAATTGTCTTATCAATAAA
TTAGCCACTCTATACCTTTT

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MQDRLISGTEKPEDHFDRAIRPTSLADYIGQPVVREQMEIFIGAARGRGEALDHTLIFGPPGLGKTTLANIIAREMGGNL
KSTSGPVLERAGDLAAMLTNLEEGDVLFIDEIHRLSPVIEEILYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVAATTR
AGLLTSPLRDRFGIVQRLEFYSVEDLTHIVSRSANLMDVPITVEGAEEVARRSRGTPRIANRLLRRVRDYAQVKGTGEVN
HEMAQRALDMLNVDKAGLDTLDRRYLSMLLERFDGGPAGVEALAAAMAEDSGTLEDVIEPYLIQQGYVMRTARGRIATNQ
SYLQFGMTPPEPKN

Sequences:

>Translated_334_residues
MQDRLISGTEKPEDHFDRAIRPTSLADYIGQPVVREQMEIFIGAARGRGEALDHTLIFGPPGLGKTTLANIIAREMGGNL
KSTSGPVLERAGDLAAMLTNLEEGDVLFIDEIHRLSPVIEEILYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVAATTR
AGLLTSPLRDRFGIVQRLEFYSVEDLTHIVSRSANLMDVPITVEGAEEVARRSRGTPRIANRLLRRVRDYAQVKGTGEVN
HEMAQRALDMLNVDKAGLDTLDRRYLSMLLERFDGGPAGVEALAAAMAEDSGTLEDVIEPYLIQQGYVMRTARGRIATNQ
SYLQFGMTPPEPKN
>Mature_334_residues
MQDRLISGTEKPEDHFDRAIRPTSLADYIGQPVVREQMEIFIGAARGRGEALDHTLIFGPPGLGKTTLANIIAREMGGNL
KSTSGPVLERAGDLAAMLTNLEEGDVLFIDEIHRLSPVIEEILYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVAATTR
AGLLTSPLRDRFGIVQRLEFYSVEDLTHIVSRSANLMDVPITVEGAEEVARRSRGTPRIANRLLRRVRDYAQVKGTGEVN
HEMAQRALDMLNVDKAGLDTLDRRYLSMLLERFDGGPAGVEALAAAMAEDSGTLEDVIEPYLIQQGYVMRTARGRIATNQ
SYLQFGMTPPEPKN

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=330, Percent_Identity=71.2121212121212, Blast_Score=473, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 36816; Mature: 36816

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDRLISGTEKPEDHFDRAIRPTSLADYIGQPVVREQMEIFIGAARGRGEALDHTLIFGP
CCCCCCCCCCCCHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCEEEECC
PGLGKTTLANIIAREMGGNLKSTSGPVLERAGDLAAMLTNLEEGDVLFIDEIHRLSPVIE
CCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHH
EILYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVAATTRAGLLTSPLRDRFGIVQRLEF
HHHHHCCCCCEEEEEECCCCCCCEEEECCCCCEEHHHHHHCCHHHHHHHHHHHHHHHHHH
YSVEDLTHIVSRSANLMDVPITVEGAEEVARRSRGTPRIANRLLRRVRDYAQVKGTGEVN
HHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHH
HEMAQRALDMLNVDKAGLDTLDRRYLSMLLERFDGGPAGVEALAAAMAEDSGTLEDVIEP
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHH
YLIQQGYVMRTARGRIATNQSYLQFGMTPPEPKN
HHHCCCCEEEECCCCCCCCCHHHEECCCCCCCCC
>Mature Secondary Structure
MQDRLISGTEKPEDHFDRAIRPTSLADYIGQPVVREQMEIFIGAARGRGEALDHTLIFGP
CCCCCCCCCCCCHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCEEEECC
PGLGKTTLANIIAREMGGNLKSTSGPVLERAGDLAAMLTNLEEGDVLFIDEIHRLSPVIE
CCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHH
EILYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVAATTRAGLLTSPLRDRFGIVQRLEF
HHHHHCCCCCEEEEEECCCCCCCEEEECCCCCEEHHHHHHCCHHHHHHHHHHHHHHHHHH
YSVEDLTHIVSRSANLMDVPITVEGAEEVARRSRGTPRIANRLLRRVRDYAQVKGTGEVN
HHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHH
HEMAQRALDMLNVDKAGLDTLDRRYLSMLLERFDGGPAGVEALAAAMAEDSGTLEDVIEP
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHH
YLIQQGYVMRTARGRIATNQSYLQFGMTPPEPKN
HHHCCCCEEEECCCCCCCCCHHHEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA