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The map label for this gene is ruvA

Identifier: 213158273

GI number: 213158273

Start: 3088742

End: 3089341

Strand: Direct

Name: ruvA

Synonym: AB57_2994

Alternate gene names: 213158273

Gene position: 3088742-3089341 (Clockwise)

Preceding gene: 213158272

Following gene: 213158274

Centisome position: 76.26

GC content: 41.67

Gene sequence:

>600_bases
ATGATCGGATGTTTAATTGGCGAAGTGTTTGCCCTAGAAGCCCCAACTGTCTTACTGAATGTAAATGGTGTGGGCTATGA
AATTGATACACCACTTTCAACATTTTGCCAATTACAAAAAGGCCAGAAAGTTACGCTATGGACGCATTTGGTCGTCCGTG
AAGATGCTCAGCAGCTTTATGGTTTTAGTGATGCTCAAGAGAAAACTATTTTCCGCACCTTACTAAAAGTAAATGGTGTG
GGCCCGAAAATGGCATTAGGTATTCTTTCTACGTTAAGTGTTGAATTGCTGGTACACACAATTGAACATGACGATGTGAA
TACTTTGGTTAAAGTTCCGGGTGTAGGCAAAAAAACAGCTGAACGTTTAATGATTGAACTTCGTGACCGTTTTAAAACTT
TGGCACAAGGAACTTCTAGCGCTGCCGCCCTGCCTCAGATTCAATTTGTTTCGAACTCACCTGTTGCAGAAGCAGAAGCA
GCCTTACAGTCACTAGGTTATAAACCTCTTGAGGCACAAAAAGCTGTTGCGGCGGTTAAAGCCGATTACACTGAATCAGC
CGATATTATTCGTGCTGCACTTAAATCAATGATGAAGTAA

Upstream 100 bases:

>100_bases
TTGTTTTAAGCTGCCTTAATATAGAAAGAAATACAGCTAGTGTCGTTTTAATACATTTAGAACGACCAGATTTACAGTTA
TGAATAAGTGAAATTACGCT

Downstream 100 bases:

>100_bases
ACACACCTATGCAAGACCGTCTAATCAGTGGAACAGAAAAACCTGAAGATCATTTTGATCGGGCTATTCGACCGACTTCA
CTTGCCGATTATATCGGGCA

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MIGCLIGEVFALEAPTVLLNVNGVGYEIDTPLSTFCQLQKGQKVTLWTHLVVREDAQQLYGFSDAQEKTIFRTLLKVNGV
GPKMALGILSTLSVELLVHTIEHDDVNTLVKVPGVGKKTAERLMIELRDRFKTLAQGTSSAAALPQIQFVSNSPVAEAEA
ALQSLGYKPLEAQKAVAAVKADYTESADIIRAALKSMMK

Sequences:

>Translated_199_residues
MIGCLIGEVFALEAPTVLLNVNGVGYEIDTPLSTFCQLQKGQKVTLWTHLVVREDAQQLYGFSDAQEKTIFRTLLKVNGV
GPKMALGILSTLSVELLVHTIEHDDVNTLVKVPGVGKKTAERLMIELRDRFKTLAQGTSSAAALPQIQFVSNSPVAEAEA
ALQSLGYKPLEAQKAVAAVKADYTESADIIRAALKSMMK
>Mature_199_residues
MIGCLIGEVFALEAPTVLLNVNGVGYEIDTPLSTFCQLQKGQKVTLWTHLVVREDAQQLYGFSDAQEKTIFRTLLKVNGV
GPKMALGILSTLSVELLVHTIEHDDVNTLVKVPGVGKKTAERLMIELRDRFKTLAQGTSSAAALPQIQFVSNSPVAEAEA
ALQSLGYKPLEAQKAVAAVKADYTESADIIRAALKSMMK

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=207, Percent_Identity=45.8937198067633, Blast_Score=179, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_ACIB3 (B7GYB6)

Other databases:

- EMBL:   CP001172
- RefSeq:   YP_002324806.1
- ProteinModelPortal:   B7GYB6
- SMR:   B7GYB6
- GeneID:   7060444
- GenomeReviews:   CP001172_GR
- KEGG:   abb:ABBFA_000893
- HOGENOM:   HBG635309
- OMA:   LFGFAQK
- ProtClustDB:   PRK00116
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 21527; Mature: 21527

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGCLIGEVFALEAPTVLLNVNGVGYEIDTPLSTFCQLQKGQKVTLWTHLVVREDAQQLY
CCHHHHHHHHHCCCCEEEEEECCCCEEECCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHC
GFSDAQEKTIFRTLLKVNGVGPKMALGILSTLSVELLVHTIEHDDVNTLVKVPGVGKKTA
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHH
ERLMIELRDRFKTLAQGTSSAAALPQIQFVSNSPVAEAEAALQSLGYKPLEAQKAVAAVK
HHHHHHHHHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
ADYTESADIIRAALKSMMK
HCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIGCLIGEVFALEAPTVLLNVNGVGYEIDTPLSTFCQLQKGQKVTLWTHLVVREDAQQLY
CCHHHHHHHHHCCCCEEEEEECCCCEEECCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHC
GFSDAQEKTIFRTLLKVNGVGPKMALGILSTLSVELLVHTIEHDDVNTLVKVPGVGKKTA
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHH
ERLMIELRDRFKTLAQGTSSAAALPQIQFVSNSPVAEAEAALQSLGYKPLEAQKAVAAVK
HHHHHHHHHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHH
ADYTESADIIRAALKSMMK
HCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA