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The map label for this gene is cysW [H]

Identifier: 213158256

GI number: 213158256

Start: 3070823

End: 3071707

Strand: Direct

Name: cysW [H]

Synonym: AB57_2976

Alternate gene names: 213158256

Gene position: 3070823-3071707 (Clockwise)

Preceding gene: 213158255

Following gene: 213158257

Centisome position: 75.81

GC content: 42.49

Gene sequence:

>885_bases
ATGAACCTACATACCGACAGCAATGCACTTGCCTTGAAATTGCAATCTCGTGATGCGACACGTGAACCAACTTGGGTACG
TTATACGCTCATTACAATTGCACTGATTTTTTTCCTTAGCTGCCTCATGCTGCCACTTATTCTGGTTTTTGTTGAAGCAT
TTAAACAAGGCCTTGAAGTCTATGTTCAAGCTTTAATTGATCCAGATACGTTATCAGCAGTGAAATTAACACTATTGACC
GCAGCAATTGCCGTTCCGATTAATGTGATATTTGGTGTTGCCGCTGCATGGGCTGTTACCAAATTTCAGTTTAAAGGTAA
AGCAATTTTAACCACCATTATTGATATGCCTTTTTCGGTTTCACCTGTTATTGCAGGTTTAATGATTGTACTGATTTTCG
GTGCTCAAGGCTGGTTAGGCGGTTGGCTCATGGAACATGACATCAAGATTCTATATGCCGTACCTGCCATTGTTTTAGCG
ACCATCTTTATTACAGTTCCATTTGTGGCGCGTGAGTTAATTCCACTTATGGAAGCGCAAGGAACTGAAGAAGAGGAAGC
AGCGATTGTACTCGGTGCATCAGGCTGGCAAACCTTTTGGAAAGTCACGCTCCCTAACATTAAATGGGGTCTTATTTACG
GCGTGATTTTGTGTAATGCCCGTGCAATGGGTGAGTTTGGGGCAGTATCAGTAGTTTCCGGCCACATCCGTGGTGAGACC
AATACCCTGCCTCTACATGTCGAAATTTTATATAACGAGTACACCTTTAGTGCAGCTTTTGCGGTGTCCTCACTACTTGC
TTTCTTGGCACTATTAACACTGATTTTAAAAACCTGGTTAGAACTACATCAAGACAAGCCAGAACCGCATTCAACTGATT
CGTAA

Upstream 100 bases:

>100_bases
CGACTATTGCAGCAGTGATGTTGGTTCTCTCTTTCATTATTTTATTTGTCATTAACTTACTTCAAGCATGGGCAAACCGT
CGTACAGGGAGAAATGTCAC

Downstream 100 bases:

>100_bases
GGAATGAAATCATGAGTATTCAAGTTAAAAATATTGAAAAACACTTTGGTGCATTCCATGCGCTTAAAAATATTTCACTC
GATTTCCCAGAGGGTGAACT

Product: sulfate ABC transporter, permease protein CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MNLHTDSNALALKLQSRDATREPTWVRYTLITIALIFFLSCLMLPLILVFVEAFKQGLEVYVQALIDPDTLSAVKLTLLT
AAIAVPINVIFGVAAAWAVTKFQFKGKAILTTIIDMPFSVSPVIAGLMIVLIFGAQGWLGGWLMEHDIKILYAVPAIVLA
TIFITVPFVARELIPLMEAQGTEEEEAAIVLGASGWQTFWKVTLPNIKWGLIYGVILCNARAMGEFGAVSVVSGHIRGET
NTLPLHVEILYNEYTFSAAFAVSSLLAFLALLTLILKTWLELHQDKPEPHSTDS

Sequences:

>Translated_294_residues
MNLHTDSNALALKLQSRDATREPTWVRYTLITIALIFFLSCLMLPLILVFVEAFKQGLEVYVQALIDPDTLSAVKLTLLT
AAIAVPINVIFGVAAAWAVTKFQFKGKAILTTIIDMPFSVSPVIAGLMIVLIFGAQGWLGGWLMEHDIKILYAVPAIVLA
TIFITVPFVARELIPLMEAQGTEEEEAAIVLGASGWQTFWKVTLPNIKWGLIYGVILCNARAMGEFGAVSVVSGHIRGET
NTLPLHVEILYNEYTFSAAFAVSSLLAFLALLTLILKTWLELHQDKPEPHSTDS
>Mature_294_residues
MNLHTDSNALALKLQSRDATREPTWVRYTLITIALIFFLSCLMLPLILVFVEAFKQGLEVYVQALIDPDTLSAVKLTLLT
AAIAVPINVIFGVAAAWAVTKFQFKGKAILTTIIDMPFSVSPVIAGLMIVLIFGAQGWLGGWLMEHDIKILYAVPAIVLA
TIFITVPFVARELIPLMEAQGTEEEEAAIVLGASGWQTFWKVTLPNIKWGLIYGVILCNARAMGEFGAVSVVSGHIRGET
NTLPLHVEILYNEYTFSAAFAVSSLLAFLALLTLILKTWLELHQDKPEPHSTDS

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=282, Percent_Identity=50.354609929078, Blast_Score=297, Evalue=6e-82,
Organism=Escherichia coli, GI1788764, Length=206, Percent_Identity=37.8640776699029, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1786980, Length=218, Percent_Identity=31.1926605504587, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787369, Length=175, Percent_Identity=26.8571428571429, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32255; Mature: 32255

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLHTDSNALALKLQSRDATREPTWVRYTLITIALIFFLSCLMLPLILVFVEAFKQGLEV
CCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVQALIDPDTLSAVKLTLLTAAIAVPINVIFGVAAAWAVTKFQFKGKAILTTIIDMPFSV
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCH
SPVIAGLMIVLIFGAQGWLGGWLMEHDIKILYAVPAIVLATIFITVPFVARELIPLMEAQ
HHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GTEEEEAAIVLGASGWQTFWKVTLPNIKWGLIYGVILCNARAMGEFGAVSVVSGHIRGET
CCCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCHHCCCCCCHHEEEEEECCCC
NTLPLHVEILYNEYTFSAAFAVSSLLAFLALLTLILKTWLELHQDKPEPHSTDS
CCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNLHTDSNALALKLQSRDATREPTWVRYTLITIALIFFLSCLMLPLILVFVEAFKQGLEV
CCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVQALIDPDTLSAVKLTLLTAAIAVPINVIFGVAAAWAVTKFQFKGKAILTTIIDMPFSV
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCH
SPVIAGLMIVLIFGAQGWLGGWLMEHDIKILYAVPAIVLATIFITVPFVARELIPLMEAQ
HHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GTEEEEAAIVLGASGWQTFWKVTLPNIKWGLIYGVILCNARAMGEFGAVSVVSGHIRGET
CCCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCHHCCCCCCHHEEEEEECCCC
NTLPLHVEILYNEYTFSAAFAVSSLLAFLALLTLILKTWLELHQDKPEPHSTDS
CCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]