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The map label for this gene is cysT [H]

Identifier: 213158255

GI number: 213158255

Start: 3070035

End: 3070826

Strand: Direct

Name: cysT [H]

Synonym: AB57_2975

Alternate gene names: 213158255

Gene position: 3070035-3070826 (Clockwise)

Preceding gene: 213158254

Following gene: 213158256

Centisome position: 75.79

GC content: 42.8

Gene sequence:

>792_bases
TTGGGCTTCACCCTCGCTTACGTTTCTTTTATTGTGCTTATTCCATTAGCCGCAGTCTTTATCAAATCATTTGGAATCGG
ATGGGACGGACTATGGGAAATTTTAACCTCTGAGCGTATTTTAAAATCGCTTCAACTCAGCTTTAGTTCAGCCTTAATTG
CGGCATTTATTAATGTTGTATTTGGGCTACTTTTAGCTTGGTGCCTTGTCCGTTATAACTTTCCCGGAAAGCGTCTGGTT
GATGCCTTAGTGGACTTACCTTTTGCACTTCCAACAGCGGTTGCAGGCATTGCGTTGACCTCACTCTATGCGCCTACAGG
CTGGATCGGTCAATATTTAGAACCTCTTGGTGTTCAGGTTGCATATACCCCTATCGGGATTACGCTAGCTTTAGTGTTTA
TCGGTATTCCTTTTATTGTTCGAACCGTTCAACCAGTATTAAGCGATATTGAAACTGAACTTGAAGAGGCGGCTTCCGCA
CTCGGTGCAAATCGCTGGCAGACCATTACCAAAATTATTTTACCAATACTACTCCCTGCCCTATTTACAGGCTTTGCTTT
AGCGTTTGCTCGCGGTGTAGGTGAATATGGTTCTGTAATTTTCATTGCAGGTAACCAGCCGTTCAAAACTGAAATTGCTC
CGCTTATGATCATTTCTCGCCTCGAAGAATATGACTATGCAGGTGCAACGACTATTGCAGCAGTGATGTTGGTTCTCTCT
TTCATTATTTTATTTGTCATTAACTTACTTCAAGCATGGGCAAACCGTCGTACAGGGAGAAATGTCACATGA

Upstream 100 bases:

>100_bases
TTTATCTTTATTTATTCGCTTACTGGTTTCAGCAGGCTTTTCTCTTTGAGGAAATATCATGTCGCAGCGATCCCGAGTGC
TGCCTGGGTTTGGCCTATCA

Downstream 100 bases:

>100_bases
ACCTACATACCGACAGCAATGCACTTGCCTTGAAATTGCAATCTCGTGATGCGACACGTGAACCAACTTGGGTACGTTAT
ACGCTCATTACAATTGCACT

Product: sulfate ABC transporter, permease protein CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MGFTLAYVSFIVLIPLAAVFIKSFGIGWDGLWEILTSERILKSLQLSFSSALIAAFINVVFGLLLAWCLVRYNFPGKRLV
DALVDLPFALPTAVAGIALTSLYAPTGWIGQYLEPLGVQVAYTPIGITLALVFIGIPFIVRTVQPVLSDIETELEEAASA
LGANRWQTITKIILPILLPALFTGFALAFARGVGEYGSVIFIAGNQPFKTEIAPLMIISRLEEYDYAGATTIAAVMLVLS
FIILFVINLLQAWANRRTGRNVT

Sequences:

>Translated_263_residues
MGFTLAYVSFIVLIPLAAVFIKSFGIGWDGLWEILTSERILKSLQLSFSSALIAAFINVVFGLLLAWCLVRYNFPGKRLV
DALVDLPFALPTAVAGIALTSLYAPTGWIGQYLEPLGVQVAYTPIGITLALVFIGIPFIVRTVQPVLSDIETELEEAASA
LGANRWQTITKIILPILLPALFTGFALAFARGVGEYGSVIFIAGNQPFKTEIAPLMIISRLEEYDYAGATTIAAVMLVLS
FIILFVINLLQAWANRRTGRNVT
>Mature_262_residues
GFTLAYVSFIVLIPLAAVFIKSFGIGWDGLWEILTSERILKSLQLSFSSALIAAFINVVFGLLLAWCLVRYNFPGKRLVD
ALVDLPFALPTAVAGIALTSLYAPTGWIGQYLEPLGVQVAYTPIGITLALVFIGIPFIVRTVQPVLSDIETELEEAASAL
GANRWQTITKIILPILLPALFTGFALAFARGVGEYGSVIFIAGNQPFKTEIAPLMIISRLEEYDYAGATTIAAVMLVLSF
IILFVINLLQAWANRRTGRNVT

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=232, Percent_Identity=51.2931034482759, Blast_Score=237, Evalue=6e-64,
Organism=Escherichia coli, GI87082099, Length=251, Percent_Identity=34.2629482071713, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1786980, Length=221, Percent_Identity=33.0316742081448, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1787368, Length=243, Percent_Identity=27.9835390946502, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786254, Length=218, Percent_Identity=26.605504587156, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787369, Length=155, Percent_Identity=28.3870967741935, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 28602; Mature: 28471

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFTLAYVSFIVLIPLAAVFIKSFGIGWDGLWEILTSERILKSLQLSFSSALIAAFINVV
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGLLLAWCLVRYNFPGKRLVDALVDLPFALPTAVAGIALTSLYAPTGWIGQYLEPLGVQV
HHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEE
AYTPIGITLALVFIGIPFIVRTVQPVLSDIETELEEAASALGANRWQTITKIILPILLPA
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LFTGFALAFARGVGEYGSVIFIAGNQPFKTEIAPLMIISRLEEYDYAGATTIAAVMLVLS
HHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
FIILFVINLLQAWANRRTGRNVT
HHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GFTLAYVSFIVLIPLAAVFIKSFGIGWDGLWEILTSERILKSLQLSFSSALIAAFINVV
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGLLLAWCLVRYNFPGKRLVDALVDLPFALPTAVAGIALTSLYAPTGWIGQYLEPLGVQV
HHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEE
AYTPIGITLALVFIGIPFIVRTVQPVLSDIETELEEAASALGANRWQTITKIILPILLPA
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LFTGFALAFARGVGEYGSVIFIAGNQPFKTEIAPLMIISRLEEYDYAGATTIAAVMLVLS
HHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
FIILFVINLLQAWANRRTGRNVT
HHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1708375 [H]