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The map label for this gene is lepA [H]

Identifier: 213158245

GI number: 213158245

Start: 3059087

End: 3060904

Strand: Reverse

Name: lepA [H]

Synonym: AB57_2964

Alternate gene names: 213158245

Gene position: 3060904-3059087 (Counterclockwise)

Preceding gene: 213158246

Following gene: 213158244

Centisome position: 75.57

GC content: 40.92

Gene sequence:

>1818_bases
ATGGCGCAAGCTAAAAAATCCGTTGATATCAAAAATATCCGAAATTTCTCGATTATCGCTCATATTGATCATGGTAAGTC
TACTTTGGCTGACCGTTTTATTCAGATGTGTGGTGGCCTGCAAGATCGTGAGATGCAAGCTCAGGTCTTGGACTCAATGG
AGCTCGAGCGTGAACGTGGGATTACCATTAAAGCTGCTTCGGTGACGTTGTACTATACTCATCCAAACGGGCAGGAATAT
CAGCTTAACTTTATTGATACTCCGGGACACGTTGATTTCTCTTATGAAGTATCACGTTCATTAGCTGCTTGTGAAGGTGC
GTTATTGGTTGTGGATGCTGCACAAGGTGTAGAAGCACAATCAGTTGCAAACTGTTATACCGCTATTGAGCAAGGTCTCG
AAGTTCTTCCAATTTTAAATAAAATTGATTTGCCGCAAGCAGAGCCTGAGCGCGTTATTCACGAAATTGAAGAAATTATT
GGTATTGAAGCAACCAATGCACCTACATGTTCTGCAAAAACAGGTTTAGGCGTTGAAGGTGTTTTAGAAACATTAGTTGA
TGTTATTCCTGCTCCAACAGGTGACCGTGAGGCTCCTTTACAGGCATTAATCATTGACTCATGGTTTGATAACTATTTAG
GTGTTGTCTCTTTAGTTCGTATTAAAGATGGTCGTATCCGCAAGGGCGACAAAATGTTGGTTAAATCTACTGGTCAGACT
CACATTGTTACATCGGTAGGTGTATTCAACCCTAAACATACTGAAACTGGCGTGCTTGAAGCTGGTGAAGTAGGCTTTGT
TATCGCGGGTATTAAAGATATTTTTGGTGCGCCGGTAGGTGATACCATCACGCTTTCAACAACCCCTGAAGTAGCTTCAC
TTCCTGGTTTTAAAAAGGTTAAACCTCAGGTTTACGCAGGTCTTTTCCCAATTGATGCAAGCGATTTTGAACCATTCCGC
GAAGCCCTACAAAAACTACAGATCAATGACTCAGCTTTATTCTTTGAACCTGAAAGTTCAGATGCGCTTGGTTTTGGTTT
CCGTTGTGGTTTCTTGGGCATGTTGCACATGGAAATCGTACAAGAGCGTTTAGAGCGCGAGTATGACCTTGATTTGATCA
GCTCAGCGCCAACGGTAGTGTATGAAGCTGTTACTAAAAAAGGCGATACGATCTATATTGATAGTCCATCTAAAATGCCG
GATGGTTCTGTTGTTGAAGATTTGCGTGAGCCAATTGCAGAGTGTCATATTCTTGTACCGCAAGAGTACTTAGGTAACGT
CATGACCCTATGTATTGAGCGCCGTGGTGTACAAAAAGACATGAAATTCTTAGGTAATCAAGTTTCAATTACTTTTGAAA
TTCCTATGGCTGAAGTGGTTATGGACTTCTTTGATAAGCTTAAATCATGTTCACGTGGTTTTGCTTCACTTGACTATAAC
TTTATTCGTTTTGAGAGTTCATCTTTAGTTAAGGTTGACGTGTTAATTAATGGTGAGAAGGTCGATGCGCTTGCAATGAT
TTGTCACCGTAATGATGCTCGTCATCGTGGTATTGCTTTAGTTGAGAAAATGAAAGATTTGATTCCACGTCAAATGTTTG
ATGTCGCAATTCAGGCTGCTATTGGTGCACAAATTATTGCTCGTTCGACCGTGAAGGCGATGCGTAAAAACGTTTTAGCA
AAATGTTATGGCGGTGACGTGTCTCGTAAGAAGAAACTACTTGCGAAACAAAAAGAAGGTAAGAAACGTATGAAACAAGT
GGGTAGTGTAGAAATCCCACAAGAAGCGTTCTTAGCTGTTTTGAAAGTAGAAAGATAA

Upstream 100 bases:

>100_bases
TAAAATATCCATCTGCTATAATCTCTCGCAATTTGTTAAACCGCTTTGGCTATGTATTAAAACAAGCTACATAGCACTTT
ATATTTTCTCAAGGTAGCCC

Downstream 100 bases:

>100_bases
GAGAAAAGGCTGGGATGAACTTATCTTCTCCCAGCCGCAAGGCAAGCAGCTAAGCTGCGAGTGAGGTCCTGTGGATTTTG
ATTTTAATTTAATTCTTGTT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 605; Mature: 604

Protein sequence:

>605_residues
MAQAKKSVDIKNIRNFSIIAHIDHGKSTLADRFIQMCGGLQDREMQAQVLDSMELERERGITIKAASVTLYYTHPNGQEY
QLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQSVANCYTAIEQGLEVLPILNKIDLPQAEPERVIHEIEEII
GIEATNAPTCSAKTGLGVEGVLETLVDVIPAPTGDREAPLQALIIDSWFDNYLGVVSLVRIKDGRIRKGDKMLVKSTGQT
HIVTSVGVFNPKHTETGVLEAGEVGFVIAGIKDIFGAPVGDTITLSTTPEVASLPGFKKVKPQVYAGLFPIDASDFEPFR
EALQKLQINDSALFFEPESSDALGFGFRCGFLGMLHMEIVQERLEREYDLDLISSAPTVVYEAVTKKGDTIYIDSPSKMP
DGSVVEDLREPIAECHILVPQEYLGNVMTLCIERRGVQKDMKFLGNQVSITFEIPMAEVVMDFFDKLKSCSRGFASLDYN
FIRFESSSLVKVDVLINGEKVDALAMICHRNDARHRGIALVEKMKDLIPRQMFDVAIQAAIGAQIIARSTVKAMRKNVLA
KCYGGDVSRKKKLLAKQKEGKKRMKQVGSVEIPQEAFLAVLKVER

Sequences:

>Translated_605_residues
MAQAKKSVDIKNIRNFSIIAHIDHGKSTLADRFIQMCGGLQDREMQAQVLDSMELERERGITIKAASVTLYYTHPNGQEY
QLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQSVANCYTAIEQGLEVLPILNKIDLPQAEPERVIHEIEEII
GIEATNAPTCSAKTGLGVEGVLETLVDVIPAPTGDREAPLQALIIDSWFDNYLGVVSLVRIKDGRIRKGDKMLVKSTGQT
HIVTSVGVFNPKHTETGVLEAGEVGFVIAGIKDIFGAPVGDTITLSTTPEVASLPGFKKVKPQVYAGLFPIDASDFEPFR
EALQKLQINDSALFFEPESSDALGFGFRCGFLGMLHMEIVQERLEREYDLDLISSAPTVVYEAVTKKGDTIYIDSPSKMP
DGSVVEDLREPIAECHILVPQEYLGNVMTLCIERRGVQKDMKFLGNQVSITFEIPMAEVVMDFFDKLKSCSRGFASLDYN
FIRFESSSLVKVDVLINGEKVDALAMICHRNDARHRGIALVEKMKDLIPRQMFDVAIQAAIGAQIIARSTVKAMRKNVLA
KCYGGDVSRKKKLLAKQKEGKKRMKQVGSVEIPQEAFLAVLKVER
>Mature_604_residues
AQAKKSVDIKNIRNFSIIAHIDHGKSTLADRFIQMCGGLQDREMQAQVLDSMELERERGITIKAASVTLYYTHPNGQEYQ
LNFIDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQSVANCYTAIEQGLEVLPILNKIDLPQAEPERVIHEIEEIIG
IEATNAPTCSAKTGLGVEGVLETLVDVIPAPTGDREAPLQALIIDSWFDNYLGVVSLVRIKDGRIRKGDKMLVKSTGQTH
IVTSVGVFNPKHTETGVLEAGEVGFVIAGIKDIFGAPVGDTITLSTTPEVASLPGFKKVKPQVYAGLFPIDASDFEPFRE
ALQKLQINDSALFFEPESSDALGFGFRCGFLGMLHMEIVQERLEREYDLDLISSAPTVVYEAVTKKGDTIYIDSPSKMPD
GSVVEDLREPIAECHILVPQEYLGNVMTLCIERRGVQKDMKFLGNQVSITFEIPMAEVVMDFFDKLKSCSRGFASLDYNF
IRFESSSLVKVDVLINGEKVDALAMICHRNDARHRGIALVEKMKDLIPRQMFDVAIQAAIGAQIIARSTVKAMRKNVLAK
CYGGDVSRKKKLLAKQKEGKKRMKQVGSVEIPQEAFLAVLKVER

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=604, Percent_Identity=49.3377483443709, Blast_Score=619, Evalue=1e-177,
Organism=Homo sapiens, GI94966754, Length=144, Percent_Identity=43.0555555555556, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI18390331, Length=183, Percent_Identity=34.9726775956284, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=38.8888888888889, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI25306283, Length=178, Percent_Identity=39.3258426966292, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI19923640, Length=178, Percent_Identity=39.3258426966292, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI25306287, Length=178, Percent_Identity=39.3258426966292, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI310132016, Length=121, Percent_Identity=42.9752066115703, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI310110807, Length=121, Percent_Identity=42.9752066115703, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI310123363, Length=121, Percent_Identity=42.9752066115703, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI217272894, Length=134, Percent_Identity=36.5671641791045, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI217272892, Length=134, Percent_Identity=36.5671641791045, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI53729339, Length=223, Percent_Identity=30.4932735426009, Blast_Score=77, Evalue=6e-14,
Organism=Homo sapiens, GI53729337, Length=223, Percent_Identity=30.4932735426009, Blast_Score=77, Evalue=6e-14,
Organism=Homo sapiens, GI34147630, Length=300, Percent_Identity=28, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI94966752, Length=94, Percent_Identity=39.3617021276596, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788922, Length=594, Percent_Identity=72.8956228956229, Blast_Score=889, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=510, Percent_Identity=28.4313725490196, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1789738, Length=189, Percent_Identity=32.8042328042328, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1790835, Length=174, Percent_Identity=32.183908045977, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789559, Length=231, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17557151, Length=610, Percent_Identity=41.9672131147541, Blast_Score=484, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17506493, Length=231, Percent_Identity=29.4372294372294, Blast_Score=99, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17556745, Length=155, Percent_Identity=32.9032258064516, Blast_Score=98, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=149, Percent_Identity=34.2281879194631, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71988811, Length=149, Percent_Identity=34.2281879194631, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=146, Percent_Identity=35.6164383561644, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=33.0827067669173, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI32566303, Length=247, Percent_Identity=28.3400809716599, Blast_Score=66, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI25141371, Length=285, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=614, Percent_Identity=45.6026058631922, Blast_Score=538, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6323098, Length=183, Percent_Identity=34.9726775956284, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324707, Length=146, Percent_Identity=38.3561643835616, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=146, Percent_Identity=38.3561643835616, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=39.1304347826087, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=173, Percent_Identity=34.6820809248555, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324761, Length=281, Percent_Identity=26.6903914590747, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325337, Length=312, Percent_Identity=26.2820512820513, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319594, Length=312, Percent_Identity=26.2820512820513, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI78706572, Length=602, Percent_Identity=42.1926910299003, Blast_Score=509, Evalue=1e-144,
Organism=Drosophila melanogaster, GI24582462, Length=187, Percent_Identity=35.2941176470588, Blast_Score=106, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24585709, Length=149, Percent_Identity=38.255033557047, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24585711, Length=149, Percent_Identity=38.255033557047, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24585713, Length=149, Percent_Identity=38.255033557047, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=42.0289855072464, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI221458488, Length=188, Percent_Identity=36.1702127659575, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357743, Length=163, Percent_Identity=33.1288343558282, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19921738, Length=279, Percent_Identity=29.3906810035842, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28572034, Length=232, Percent_Identity=29.7413793103448, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66659; Mature: 66528

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQAKKSVDIKNIRNFSIIAHIDHGKSTLADRFIQMCGGLQDREMQAQVLDSMELERERG
CCCCCCCCCHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
ITIKAASVTLYYTHPNGQEYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQ
CEEEEEEEEEEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHHCCCEEEEECCCCCCHH
SVANCYTAIEQGLEVLPILNKIDLPQAEPERVIHEIEEIIGIEATNAPTCSAKTGLGVEG
HHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
VLETLVDVIPAPTGDREAPLQALIIDSWFDNYLGVVSLVRIKDGRIRKGDKMLVKSTGQT
HHHHHHHHHCCCCCCCCCCHHEEEHHHHHHHHHHHHEEEEECCCCCCCCCEEEEECCCCE
HIVTSVGVFNPKHTETGVLEAGEVGFVIAGIKDIFGAPVGDTITLSTTPEVASLPGFKKV
EEEEEEECCCCCCCCCCCEECCCCCEEEECHHHHHCCCCCCEEEEECCCCHHHCCCCHHC
KPQVYAGLFPIDASDFEPFREALQKLQINDSALFFEPESSDALGFGFRCGFLGMLHMEIV
CCHHHCCEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHH
QERLEREYDLDLISSAPTVVYEAVTKKGDTIYIDSPSKMPDGSVVEDLREPIAECHILVP
HHHHHHHCCCHHHCCCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCEEECC
QEYLGNVMTLCIERRGVQKDMKFLGNQVSITFEIPMAEVVMDFFDKLKSCSRGFASLDYN
HHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHCCHHHCCCE
FIRFESSSLVKVDVLINGEKVDALAMICHRNDARHRGIALVEKMKDLIPRQMFDVAIQAA
EEEECCCCEEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IGAQIIARSTVKAMRKNVLAKCYGGDVSRKKKLLAKQKEGKKRMKQVGSVEIPQEAFLAV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LKVER
HEECC
>Mature Secondary Structure 
AQAKKSVDIKNIRNFSIIAHIDHGKSTLADRFIQMCGGLQDREMQAQVLDSMELERERG
CCCCCCCCHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
ITIKAASVTLYYTHPNGQEYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQ
CEEEEEEEEEEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHHCCCEEEEECCCCCCHH
SVANCYTAIEQGLEVLPILNKIDLPQAEPERVIHEIEEIIGIEATNAPTCSAKTGLGVEG
HHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
VLETLVDVIPAPTGDREAPLQALIIDSWFDNYLGVVSLVRIKDGRIRKGDKMLVKSTGQT
HHHHHHHHHCCCCCCCCCCHHEEEHHHHHHHHHHHHEEEEECCCCCCCCCEEEEECCCCE
HIVTSVGVFNPKHTETGVLEAGEVGFVIAGIKDIFGAPVGDTITLSTTPEVASLPGFKKV
EEEEEEECCCCCCCCCCCEECCCCCEEEECHHHHHCCCCCCEEEEECCCCHHHCCCCHHC
KPQVYAGLFPIDASDFEPFREALQKLQINDSALFFEPESSDALGFGFRCGFLGMLHMEIV
CCHHHCCEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHH
QERLEREYDLDLISSAPTVVYEAVTKKGDTIYIDSPSKMPDGSVVEDLREPIAECHILVP
HHHHHHHCCCHHHCCCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCEEECC
QEYLGNVMTLCIERRGVQKDMKFLGNQVSITFEIPMAEVVMDFFDKLKSCSRGFASLDYN
HHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHCCHHHCCCE
FIRFESSSLVKVDVLINGEKVDALAMICHRNDARHRGIALVEKMKDLIPRQMFDVAIQAA
EEEECCCCEEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IGAQIIARSTVKAMRKNVLAKCYGGDVSRKKKLLAKQKEGKKRMKQVGSVEIPQEAFLAV
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LKVER
HEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA