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The map label for this gene is entE [H]

Identifier: 213158098

GI number: 213158098

Start: 2889101

End: 2890729

Strand: Reverse

Name: entE [H]

Synonym: AB57_2810

Alternate gene names: 213158098

Gene position: 2890729-2889101 (Counterclockwise)

Preceding gene: 213158101

Following gene: 213158097

Centisome position: 71.37

GC content: 40.95

Gene sequence:

>1629_bases
ATGAAAAAACAGTTGATTGAGTTTGTTCGCTGGTCTCCCGAGAGAGCGCAACACTATCGAAATAAAGGTTATTGGATTGA
CCAACCTTTGACGCGCATTTTAACGGTAGGGGTTCAATCGCATCCGCATTCGCCGGCAATTATTTGTGGTGAACGTCAGT
TGAGTTATATCGAGCTGGATCGGCTATCAACAAATTTAGCTTCTCGACTAGCTGAAAAAGGCTTGGGCAAGGGTGATACT
GCTTTAGTACAACTTCCCAATGTTGCGGAGTTTTATATTGTCTTTTTTGCCTTGCTTAAAGCAGGCGTGGTTATGCTCAA
TGCGCTATATAGCCATCGCCAATATGAACTAAATGCCTTCATTAAACAGATTCAACCTAAGCTTTTAATTGGTTCGCGTC
AGCATGAAGTCTTTAGCAATAATCAATTTATTGACTCACTTCATGAAGTAAATTTAAGTCCTGAAATTATTTTGATGTTC
AATCATCAAGCTACCGATTTTGGATTATTAGACTGGATTGAAACACCAGTAGAGACCTTTGTCGATTTTTCATCTACACC
TGCTGATGAAGTTGCTTTCTTTCAGTTGTCAGGTGGAAGTACGGGAACACCAAAACTTATTCCACGCACGCATAATGACT
ATGACTATAGTGTGCGAGCCAGTGCCGAGATTTGCGGTTTAAACTCAAATACCCGGTTATTATGTGCTCTGCCAGCTCCG
CATAACTTTATGTTGAGCTCACCTGGTGCATTAGGTGTTTTACATGCAGGTGGATGTGTTGTTATGGCACCAAATCCTGA
ACCGCTCAATTGTTTTTCTATTATTCAAAGACATCAAGTCAATATGGCATCTTTAGTGCCGAGTGCTGTCATTATGTGGC
TAGAAAAAGCCGCGCAATATAAAGATCAAATTCAGTCTTTAAAGTTGCTTCAAGTAGGTGGAGCAAGTTTCCCTGAATCT
TTAGCCCGTCAAGTTCCTGAAGTCCTCAATTGTAAATTACAACAAGTATTTGGAATGGCCGAAGGCTTGGTCAATTACAC
CAGACTCGATGACTCCGACGAGCAAATCTTTACGACACAAGGGCGTCCTATCAGTTCTGATGATGAAATCAAAATTGTAG
ATGAGCAGTATAGAGAAGTTCCGGAAGGTGAAGTAGGAATGCTTGCGACTCGGGGGCCTTATACCTTTTGTGGTTATTAC
CAAAGCCCTGAACATAATTCACAGGTCTTTGATGAGGACAACTATTACTATTCGGGCGATCTCGTGCAGCGTACCCATGA
TGGCAATTTACGTGTAGTAGGAAGAATTAAAGACCAAATTAACCGTGGTGGTGAAAAAATTGCTTCGGAAGAAATAGAAA
AACTTATTCTTTTACATCCGGAAGTTATGCACGCAGCTTTGGTTGCAATTGTTGATGAACAATTCGGTGAAAAAAGTTGT
GCCTTTATTGTTTCTCGGAATCCTGAACTTAAAGCTGTTGTGCTCAGACGCCATCTCATGGAGTTGGGTATTGCACAATA
TAAGCTTCCAGACCAGATCAAATTAATCGAATGTTTGCCACTGACCGCAGTCGGTAAGGTGGACAAAAAACAACTTCGCA
GCATTTTAAATACATCTACAACATCTTAA

Upstream 100 bases:

>100_bases
AGTCGGTATTCAAATTAAATATTTATGCCGAGATGTAAGAAAATTTACCAAGAATCAATAGCTTGATAACCATTAAATTA
AATTGAATATTTAGAATGTC

Downstream 100 bases:

>100_bases
TAAATATTGAGCAGCATATGGCTATTTCAAAAATTTCTACTTATCTCATGCCCGAAAGAGAGAGTTATCCAAATAATAAA
ACAGACTGGCAATTAGATCC

Product: enterobactin synthase subunit E

Products: NA

Alternate protein names: Enterochelin synthase E; 2,3-dihydroxybenzoate-AMP ligase; Dihydroxybenzoic acid-activating enzyme; S-dihydroxybenzoyltransferase [H]

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MKKQLIEFVRWSPERAQHYRNKGYWIDQPLTRILTVGVQSHPHSPAIICGERQLSYIELDRLSTNLASRLAEKGLGKGDT
ALVQLPNVAEFYIVFFALLKAGVVMLNALYSHRQYELNAFIKQIQPKLLIGSRQHEVFSNNQFIDSLHEVNLSPEIILMF
NHQATDFGLLDWIETPVETFVDFSSTPADEVAFFQLSGGSTGTPKLIPRTHNDYDYSVRASAEICGLNSNTRLLCALPAP
HNFMLSSPGALGVLHAGGCVVMAPNPEPLNCFSIIQRHQVNMASLVPSAVIMWLEKAAQYKDQIQSLKLLQVGGASFPES
LARQVPEVLNCKLQQVFGMAEGLVNYTRLDDSDEQIFTTQGRPISSDDEIKIVDEQYREVPEGEVGMLATRGPYTFCGYY
QSPEHNSQVFDEDNYYYSGDLVQRTHDGNLRVVGRIKDQINRGGEKIASEEIEKLILLHPEVMHAALVAIVDEQFGEKSC
AFIVSRNPELKAVVLRRHLMELGIAQYKLPDQIKLIECLPLTAVGKVDKKQLRSILNTSTTS

Sequences:

>Translated_542_residues
MKKQLIEFVRWSPERAQHYRNKGYWIDQPLTRILTVGVQSHPHSPAIICGERQLSYIELDRLSTNLASRLAEKGLGKGDT
ALVQLPNVAEFYIVFFALLKAGVVMLNALYSHRQYELNAFIKQIQPKLLIGSRQHEVFSNNQFIDSLHEVNLSPEIILMF
NHQATDFGLLDWIETPVETFVDFSSTPADEVAFFQLSGGSTGTPKLIPRTHNDYDYSVRASAEICGLNSNTRLLCALPAP
HNFMLSSPGALGVLHAGGCVVMAPNPEPLNCFSIIQRHQVNMASLVPSAVIMWLEKAAQYKDQIQSLKLLQVGGASFPES
LARQVPEVLNCKLQQVFGMAEGLVNYTRLDDSDEQIFTTQGRPISSDDEIKIVDEQYREVPEGEVGMLATRGPYTFCGYY
QSPEHNSQVFDEDNYYYSGDLVQRTHDGNLRVVGRIKDQINRGGEKIASEEIEKLILLHPEVMHAALVAIVDEQFGEKSC
AFIVSRNPELKAVVLRRHLMELGIAQYKLPDQIKLIECLPLTAVGKVDKKQLRSILNTSTTS
>Mature_542_residues
MKKQLIEFVRWSPERAQHYRNKGYWIDQPLTRILTVGVQSHPHSPAIICGERQLSYIELDRLSTNLASRLAEKGLGKGDT
ALVQLPNVAEFYIVFFALLKAGVVMLNALYSHRQYELNAFIKQIQPKLLIGSRQHEVFSNNQFIDSLHEVNLSPEIILMF
NHQATDFGLLDWIETPVETFVDFSSTPADEVAFFQLSGGSTGTPKLIPRTHNDYDYSVRASAEICGLNSNTRLLCALPAP
HNFMLSSPGALGVLHAGGCVVMAPNPEPLNCFSIIQRHQVNMASLVPSAVIMWLEKAAQYKDQIQSLKLLQVGGASFPES
LARQVPEVLNCKLQQVFGMAEGLVNYTRLDDSDEQIFTTQGRPISSDDEIKIVDEQYREVPEGEVGMLATRGPYTFCGYY
QSPEHNSQVFDEDNYYYSGDLVQRTHDGNLRVVGRIKDQINRGGEKIASEEIEKLILLHPEVMHAALVAIVDEQFGEKSC
AFIVSRNPELKAVVLRRHLMELGIAQYKLPDQIKLIECLPLTAVGKVDKKQLRSILNTSTTS

Specific function: Activates the carboxylate group of 2,3-dihydroxy- benzoate (2,3-DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate. Then, catalyzes the acylation of holo-EntB with 2,3-DHB adenylate, preparing that molecule for amide bond formation with

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. EntE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=514, Percent_Identity=24.3190661478599, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI38505220, Length=501, Percent_Identity=22.5548902195609, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI58082049, Length=511, Percent_Identity=22.8962818003914, Blast_Score=106, Evalue=7e-23,
Organism=Homo sapiens, GI157311624, Length=502, Percent_Identity=22.9083665338645, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI157311622, Length=502, Percent_Identity=22.9083665338645, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI42544132, Length=516, Percent_Identity=23.2558139534884, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI28416953, Length=554, Percent_Identity=23.4657039711191, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI187761345, Length=378, Percent_Identity=24.3386243386243, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI187761343, Length=378, Percent_Identity=24.3386243386243, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI122937307, Length=522, Percent_Identity=22.9885057471264, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI115511026, Length=505, Percent_Identity=23.1683168316832, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1786810, Length=539, Percent_Identity=55.2875695732839, Blast_Score=590, Evalue=1e-169,
Organism=Escherichia coli, GI145693145, Length=542, Percent_Identity=29.8892988929889, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1788107, Length=541, Percent_Identity=24.7689463955638, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI221142682, Length=505, Percent_Identity=24.1584158415842, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1790505, Length=534, Percent_Identity=23.7827715355805, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1786529, Length=533, Percent_Identity=22.1388367729831, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1786801, Length=451, Percent_Identity=23.2815964523282, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789201, Length=364, Percent_Identity=23.0769230769231, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17557194, Length=511, Percent_Identity=28.5714285714286, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17538037, Length=518, Percent_Identity=27.2200772200772, Blast_Score=138, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI71994703, Length=518, Percent_Identity=26.4478764478764, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71994694, Length=518, Percent_Identity=26.4478764478764, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71994690, Length=520, Percent_Identity=26.1538461538462, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17560308, Length=520, Percent_Identity=26.3461538461538, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17559526, Length=518, Percent_Identity=25.4826254826255, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=532, Percent_Identity=22.5563909774436, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71996755, Length=503, Percent_Identity=23.6580516898608, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI32563687, Length=502, Percent_Identity=23.7051792828685, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17558820, Length=507, Percent_Identity=23.4714003944773, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=292, Percent_Identity=25.3424657534247, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71985884, Length=275, Percent_Identity=26.5454545454545, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17507435, Length=526, Percent_Identity=22.6235741444867, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=359, Percent_Identity=24.2339832869081, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319264, Length=514, Percent_Identity=23.1517509727626, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24581924, Length=526, Percent_Identity=23.0038022813688, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI18859661, Length=558, Percent_Identity=23.1182795698925, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21355181, Length=490, Percent_Identity=23.8775510204082, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19922652, Length=501, Percent_Identity=22.7544910179641, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21358303, Length=447, Percent_Identity=22.5950782997763, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24653035, Length=550, Percent_Identity=21.4545454545455, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24656500, Length=511, Percent_Identity=23.091976516634, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21356947, Length=416, Percent_Identity=21.1538461538462, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI161076582, Length=563, Percent_Identity=21.4920071047957, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648260, Length=274, Percent_Identity=24.4525547445255, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI281365686, Length=437, Percent_Identity=24.9427917620137, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356441, Length=491, Percent_Identity=21.5885947046843, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI20130357, Length=359, Percent_Identity=22.8412256267409, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24648253, Length=548, Percent_Identity=21.3503649635036, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648255, Length=548, Percent_Identity=21.5328467153285, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24648676, Length=378, Percent_Identity=22.7513227513227, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =2.7.7.58 [H]

Molecular weight: Translated: 60729; Mature: 60729

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQLIEFVRWSPERAQHYRNKGYWIDQPLTRILTVGVQSHPHSPAIICGERQLSYIELD
CHHHHHHHHHCCHHHHHHHCCCCEECCHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEHH
RLSTNLASRLAEKGLGKGDTALVQLPNVAEFYIVFFALLKAGVVMLNALYSHRQYELNAF
HHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
IKQIQPKLLIGSRQHEVFSNNQFIDSLHEVNLSPEIILMFNHQATDFGLLDWIETPVETF
HHHCCCCEEECCCHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHH
VDFSSTPADEVAFFQLSGGSTGTPKLIPRTHNDYDYSVRASAEICGLNSNTRLLCALPAP
HCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCEEEECCCCCCEEEEEECCC
HNFMLSSPGALGVLHAGGCVVMAPNPEPLNCFSIIQRHQVNMASLVPSAVIMWLEKAAQY
CCEEECCCCCEEEEECCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KDQIQSLKLLQVGGASFPESLARQVPEVLNCKLQQVFGMAEGLVNYTRLDDSDEQIFTTQ
HHHHHHHHHHEECCCCCHHHHHHHCHHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
GRPISSDDEIKIVDEQYREVPEGEVGMLATRGPYTFCGYYQSPEHNSQVFDEDNYYYSGD
CCCCCCCCCEEEECHHHHCCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCEECCC
LVQRTHDGNLRVVGRIKDQINRGGEKIASEEIEKLILLHPEVMHAALVAIVDEQFGEKSC
CEEEECCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCE
AFIVSRNPELKAVVLRRHLMELGIAQYKLPDQIKLIECLPLTAVGKVDKKQLRSILNTST
EEEEECCCCHHHHHHHHHHHHHCHHHCCCCCCEEEEEECCCHHCCCCCHHHHHHHHHCCC
TS
CC
>Mature Secondary Structure
MKKQLIEFVRWSPERAQHYRNKGYWIDQPLTRILTVGVQSHPHSPAIICGERQLSYIELD
CHHHHHHHHHCCHHHHHHHCCCCEECCHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEHH
RLSTNLASRLAEKGLGKGDTALVQLPNVAEFYIVFFALLKAGVVMLNALYSHRQYELNAF
HHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
IKQIQPKLLIGSRQHEVFSNNQFIDSLHEVNLSPEIILMFNHQATDFGLLDWIETPVETF
HHHCCCCEEECCCHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHH
VDFSSTPADEVAFFQLSGGSTGTPKLIPRTHNDYDYSVRASAEICGLNSNTRLLCALPAP
HCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCEEEECCCCCCEEEEEECCC
HNFMLSSPGALGVLHAGGCVVMAPNPEPLNCFSIIQRHQVNMASLVPSAVIMWLEKAAQY
CCEEECCCCCEEEEECCCEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KDQIQSLKLLQVGGASFPESLARQVPEVLNCKLQQVFGMAEGLVNYTRLDDSDEQIFTTQ
HHHHHHHHHHEECCCCCHHHHHHHCHHHHCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
GRPISSDDEIKIVDEQYREVPEGEVGMLATRGPYTFCGYYQSPEHNSQVFDEDNYYYSGD
CCCCCCCCCEEEECHHHHCCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCEECCC
LVQRTHDGNLRVVGRIKDQINRGGEKIASEEIEKLILLHPEVMHAALVAIVDEQFGEKSC
CEEEECCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCE
AFIVSRNPELKAVVLRRHLMELGIAQYKLPDQIKLIECLPLTAVGKVDKKQLRSILNTST
EEEEECCCCHHHHHHHHHHHHHCHHHCCCCCCEEEEEECCCHHCCCCCHHHHHHHHHCCC
TS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]