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The map label for this gene is entB [H]

Identifier: 213158097

GI number: 213158097

Start: 2888214

End: 2889083

Strand: Reverse

Name: entB [H]

Synonym: AB57_2809

Alternate gene names: 213158097

Gene position: 2889083-2888214 (Counterclockwise)

Preceding gene: 213158098

Following gene: 213158096

Centisome position: 71.33

GC content: 40.69

Gene sequence:

>870_bases
ATGGCTATTTCAAAAATTTCTACTTATCTCATGCCCGAAAGAGAGAGTTATCCAAATAATAAAACAGACTGGCAATTAGA
TCCTTCTCGAGCAGTTTTATTAATTCATGATATGCAGCGTTACTTTTTAAATTTTTACGATGCTGAAAGTGAATTAATTA
AAACGGTTGTTAATCATTTAGTTCAACTAAGAACTTGGGCACATCAGAACAATGTTCCTGTTGTCTATACAGCACAGCCT
TATGAACAGCCAGCGGCAGATCGTGCATTACTGAATGCCATGTGGGGACCGGGTTTGCCAGCCTCAACAATCGATCAGCA
AAAAATTATTGATCAACTTTCTCCTGCTGAACATGACATTGTCTTAACGAAATGGCGTTATAGCGCGTTTAAACGTTCGG
ATTTGCTTGAAAGAATGCAAAACTGGAATCGTGATCAGCTCATTATTGGCGGTGTCTATGCCCATATCGGTTGTATGGTT
ACGGCAATTGAAGCTTTTATGAGTGACATACAACCTTTTCTGGTTGGAGATGCAGTTGCCGATTTTTCAGAAGAAGAACA
TCGCTTAGCTTTGAAATATGTATCTTCACGTTGCGGTCAAGTTGTGGATACCGAATCTGTAGTGGGGCAGGTGGCAACAG
GCATTACACGACCTTGGCTAGAGCAAAAGGTCCAGCAGCTTATTGAAGAAGATGGATTAGATCCAGAAGAGAACCTGATT
CTTTATGGACTGGACTCATTACGAATTATGCAGTTTTCTTCGGAACTCAAAGCGCAGGGAATCAATATTAGTTTTGAAGA
ATTAGGACGCACACCCACTTTATCAAATTGGTGGTCATTGGTTGATGCACGGCAAAGGATTGCAGGGTAG

Upstream 100 bases:

>100_bases
TCGAATGTTTGCCACTGACCGCAGTCGGTAAGGTGGACAAAAAACAACTTCGCAGCATTTTAAATACATCTACAACATCT
TAATAAATATTGAGCAGCAT

Downstream 100 bases:

>100_bases
AGGGTTGCCATCATAAGGCATACGACAAATCCTGGTCGCTAAATTTATACATAAAATTTATATCTTTGATAGCGACTCCT
TAGACGACTTGTAATCGTCA

Product: 2,3 dihydro-2,3 dihydroxybenzoate synthase

Products: NA

Alternate protein names: 2,3 dihydro-2,3 dihydroxybenzoate synthase; Enterobactin synthase component B; Enterochelin synthase B; Isochorismate lyase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MAISKISTYLMPERESYPNNKTDWQLDPSRAVLLIHDMQRYFLNFYDAESELIKTVVNHLVQLRTWAHQNNVPVVYTAQP
YEQPAADRALLNAMWGPGLPASTIDQQKIIDQLSPAEHDIVLTKWRYSAFKRSDLLERMQNWNRDQLIIGGVYAHIGCMV
TAIEAFMSDIQPFLVGDAVADFSEEEHRLALKYVSSRCGQVVDTESVVGQVATGITRPWLEQKVQQLIEEDGLDPEENLI
LYGLDSLRIMQFSSELKAQGINISFEELGRTPTLSNWWSLVDARQRIAG

Sequences:

>Translated_289_residues
MAISKISTYLMPERESYPNNKTDWQLDPSRAVLLIHDMQRYFLNFYDAESELIKTVVNHLVQLRTWAHQNNVPVVYTAQP
YEQPAADRALLNAMWGPGLPASTIDQQKIIDQLSPAEHDIVLTKWRYSAFKRSDLLERMQNWNRDQLIIGGVYAHIGCMV
TAIEAFMSDIQPFLVGDAVADFSEEEHRLALKYVSSRCGQVVDTESVVGQVATGITRPWLEQKVQQLIEEDGLDPEENLI
LYGLDSLRIMQFSSELKAQGINISFEELGRTPTLSNWWSLVDARQRIAG
>Mature_288_residues
AISKISTYLMPERESYPNNKTDWQLDPSRAVLLIHDMQRYFLNFYDAESELIKTVVNHLVQLRTWAHQNNVPVVYTAQPY
EQPAADRALLNAMWGPGLPASTIDQQKIIDQLSPAEHDIVLTKWRYSAFKRSDLLERMQNWNRDQLIIGGVYAHIGCMVT
AIEAFMSDIQPFLVGDAVADFSEEEHRLALKYVSSRCGQVVDTESVVGQVATGITRPWLEQKVQQLIEEDGLDPEENLIL
YGLDSLRIMQFSSELKAQGINISFEELGRTPTLSNWWSLVDARQRIAG

Specific function: Required for production of 2,3-DHB. Also serves as an aryl carrier protein and plays a role in enterobactin assembly [H]

COG id: COG1535

COG function: function code Q; Isochorismate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Escherichia coli, GI1786811, Length=281, Percent_Identity=48.0427046263345, Blast_Score=273, Evalue=9e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009081
- InterPro:   IPR016291
- InterPro:   IPR000868
- InterPro:   IPR006163 [H]

Pfam domain/function: PF00857 Isochorismatase; PF00550 PP-binding [H]

EC number: =3.3.2.1 [H]

Molecular weight: Translated: 32956; Mature: 32825

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50075 ACP_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISKISTYLMPERESYPNNKTDWQLDPSRAVLLIHDMQRYFLNFYDAESELIKTVVNHL
CCHHHHHHHHCCCHHCCCCCCCCCEECCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHH
VQLRTWAHQNNVPVVYTAQPYEQPAADRALLNAMWGPGLPASTIDQQKIIDQLSPAEHDI
HHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCE
VLTKWRYSAFKRSDLLERMQNWNRDQLIIGGVYAHIGCMVTAIEAFMSDIQPFLVGDAVA
EEEEHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
DFSEEEHRLALKYVSSRCGQVVDTESVVGQVATGITRPWLEQKVQQLIEEDGLDPEENLI
HCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEE
LYGLDSLRIMQFSSELKAQGINISFEELGRTPTLSNWWSLVDARQRIAG
EEECCHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AISKISTYLMPERESYPNNKTDWQLDPSRAVLLIHDMQRYFLNFYDAESELIKTVVNHL
CHHHHHHHHCCCHHCCCCCCCCCEECCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHH
VQLRTWAHQNNVPVVYTAQPYEQPAADRALLNAMWGPGLPASTIDQQKIIDQLSPAEHDI
HHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCE
VLTKWRYSAFKRSDLLERMQNWNRDQLIIGGVYAHIGCMVTAIEAFMSDIQPFLVGDAVA
EEEEHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
DFSEEEHRLALKYVSSRCGQVVDTESVVGQVATGITRPWLEQKVQQLIEEDGLDPEENLI
HCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEE
LYGLDSLRIMQFSSELKAQGINISFEELGRTPTLSNWWSLVDARQRIAG
EEECCHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]