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The map label for this gene is trpA

Identifier: 213157883

GI number: 213157883

Start: 3478679

End: 3479482

Strand: Reverse

Name: trpA

Synonym: AB57_3373

Alternate gene names: 213157883

Gene position: 3479482-3478679 (Counterclockwise)

Preceding gene: 213157884

Following gene: 213157882

Centisome position: 85.9

GC content: 44.9

Gene sequence:

>804_bases
ATGTCACGTCTTGCCACCCGTTTTGAAAAGCTGCAGTCCCAACAACGTAAAGCGCTTGTATCTTATGTCATGGCGGGTGA
TCCACAGCCACAAGTAACGGTGCCGTTACTTCATAAAATGGTTGCGGCAGGAGTAGACGTCATTGAGCTTGGTTTACCAT
TTTCAGACCCGATGGCTGATGGTCCGGTAATTGCACTTGCTGCTGAACGCGCACTTGCGGCTGGGACGAACACCTTAGAT
GCGCTGAACATGGTAAAAGAGTTTCGTGAGCAGGATCAGGAAACACCTGTAGTTTTAATGGGTTATCTTAATCCTGTTGA
AGTGATTGGTTACGAGAAGTTTGTTTCTTATGCGAAACAATGTGGTGTAGATGGCTTATTACTTGTGGACTTACCACCGG
AAGAGTCAAAAGAATTTGGTGCAATCTTGAAACAACATGATATGGATCAGATTTTCTTACTTGCACCAACTTCAACTGAT
CAACGTATTCAACACGTAGCGAATCAGGCAAGCGGGTTTATCTACTATGTTTCACTCAAAGGTGTAACAGGTGCAGCGAC
TTTGGATACTTCTGAAGCGGCTGCACGTATTGAAAAAATCAAAGGTATGACTAACGTTCCAGTTGGCGTTGGTTTCGGAA
TTAGTGATGCTGCTTCAGCGAAAGCGATGGGTAGTGTTGCAGATGCCGTGATTGTAGGTAGTGCTTTCGTTAAATCTTTT
GCAACGTTGGCAGCTGATGAAGCTGTTGAACAGACGGTGAATAAAGTCAAGGAGCTTCGAGCAGCGCTCGATGAGTTAGT
ATGA

Upstream 100 bases:

>100_bases
AGACCTGTTCAATGAACAAAAAATAGCGTTAAAATGCGGTGTTCTTGTATTTCTACAAACAACTCCTCAATTTTAAATAA
TTCAAGGAAAGCACAACCCT

Downstream 100 bases:

>100_bases
ATCAAGAAGTGAAATCAGGGAAAGTTCTTAGCCCTTCGACGCCATGGACACAGCGTCCAGTTCCAGGTATTGAAGTTGCA
GATGAGCAACAGACTCTCAA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSRLATRFEKLQSQQRKALVSYVMAGDPQPQVTVPLLHKMVAAGVDVIELGLPFSDPMADGPVIALAAERALAAGTNTLD
ALNMVKEFREQDQETPVVLMGYLNPVEVIGYEKFVSYAKQCGVDGLLLVDLPPEESKEFGAILKQHDMDQIFLLAPTSTD
QRIQHVANQASGFIYYVSLKGVTGAATLDTSEAAARIEKIKGMTNVPVGVGFGISDAASAKAMGSVADAVIVGSAFVKSF
ATLAADEAVEQTVNKVKELRAALDELV

Sequences:

>Translated_267_residues
MSRLATRFEKLQSQQRKALVSYVMAGDPQPQVTVPLLHKMVAAGVDVIELGLPFSDPMADGPVIALAAERALAAGTNTLD
ALNMVKEFREQDQETPVVLMGYLNPVEVIGYEKFVSYAKQCGVDGLLLVDLPPEESKEFGAILKQHDMDQIFLLAPTSTD
QRIQHVANQASGFIYYVSLKGVTGAATLDTSEAAARIEKIKGMTNVPVGVGFGISDAASAKAMGSVADAVIVGSAFVKSF
ATLAADEAVEQTVNKVKELRAALDELV
>Mature_266_residues
SRLATRFEKLQSQQRKALVSYVMAGDPQPQVTVPLLHKMVAAGVDVIELGLPFSDPMADGPVIALAAERALAAGTNTLDA
LNMVKEFREQDQETPVVLMGYLNPVEVIGYEKFVSYAKQCGVDGLLLVDLPPEESKEFGAILKQHDMDQIFLLAPTSTDQ
RIQHVANQASGFIYYVSLKGVTGAATLDTSEAAARIEKIKGMTNVPVGVGFGISDAASAKAMGSVADAVIVGSAFVKSFA
TLAADEAVEQTVNKVKELRAALDELV

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=268, Percent_Identity=32.8358208955224, Blast_Score=140, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321412, Length=245, Percent_Identity=38.3673469387755, Blast_Score=177, Evalue=1e-45,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_ACIB3 (B7GWQ5)

Other databases:

- EMBL:   CP001172
- RefSeq:   YP_002324516.1
- ProteinModelPortal:   B7GWQ5
- SMR:   B7GWQ5
- GeneID:   7059579
- GenomeReviews:   CP001172_GR
- KEGG:   abb:ABBFA_000592
- HOGENOM:   HBG391690
- OMA:   VILMTYY
- ProtClustDB:   PRK13111
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28441; Mature: 28310

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLATRFEKLQSQQRKALVSYVMAGDPQPQVTVPLLHKMVAAGVDVIELGLPFSDPMAD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIALAAERALAAGTNTLDALNMVKEFREQDQETPVVLMGYLNPVEVIGYEKFVSYAKQ
CCEEEEEHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
CGVDGLLLVDLPPEESKEFGAILKQHDMDQIFLLAPTSTDQRIQHVANQASGFIYYVSLK
CCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEEEC
GVTGAATLDTSEAAARIEKIKGMTNVPVGVGFGISDAASAKAMGSVADAVIVGSAFVKSF
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ATLAADEAVEQTVNKVKELRAALDELV
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRLATRFEKLQSQQRKALVSYVMAGDPQPQVTVPLLHKMVAAGVDVIELGLPFSDPMAD
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIALAAERALAAGTNTLDALNMVKEFREQDQETPVVLMGYLNPVEVIGYEKFVSYAKQ
CCEEEEEHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
CGVDGLLLVDLPPEESKEFGAILKQHDMDQIFLLAPTSTDQRIQHVANQASGFIYYVSLK
CCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEEEC
GVTGAATLDTSEAAARIEKIKGMTNVPVGVGFGISDAASAKAMGSVADAVIVGSAFVKSF
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ATLAADEAVEQTVNKVKELRAALDELV
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA