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The map label for this gene is gatB [H]

Identifier: 213157803

GI number: 213157803

Start: 3384896

End: 3386365

Strand: Direct

Name: gatB [H]

Synonym: AB57_3286

Alternate gene names: 213157803

Gene position: 3384896-3386365 (Clockwise)

Preceding gene: 213157802

Following gene: 213157804

Centisome position: 83.57

GC content: 43.2

Gene sequence:

>1470_bases
ATGGCTGAAGCTCAAAAGTTGAAATTGATTGACGGTTGGGAAGTCGTTATTGGTATTGAGATTCACACTCAGCTTGCAAC
CAATTCTAAAATTTTCTCAGGTTCATCAACTGAATTTGGTCAAGACCCAAATACACAAGCGAGCCTTGTAGACTTGGCGA
TGCCGGGTGTATTGCCGGTTTTAAATGAAAAAGTGGTTGAACTCGCAATTCGTTTTGGTTTAGGGATTGATGCTTACATC
GACCAAGCTTCAGTATTTGCACGTAAAAACTACTTCTATCCTGACTCTCCAAAAGGCTATCAAATTAGCCAAATGGATAA
CCCGATTGTGGGCTTGGGCCATATCGACATTCAGCTTGAAGATGGTACGACTAAACGCATTGGCGTAACTCGTGCTCACC
TTGAAGAAGATGCGGGTAAGTCTGTGCACGACCAATTTGAAGGTATGTCTGGCATTGACTTAAACCGTGCTGGTACGCCT
TTGCTTGAAATCGTTTCTGAACCAGACATGCGTTCAGTTGAAGAAGCAGTTGCCTACATCAAAGCAATTCACACACTAGT
ACGTTGGTTAGGTATTTCTGACGGTAACATGGCTGAAGGATCATTCCGTGCGGACTGTAACGTGTCTTTACGTCGTCCGG
GACAGCCATTCGGTACACGCTGTGAGCTCAAAAACCTCAACTCATTCCGTTTCATTGAGCAAGCGATCAATGTTGAAATT
GAACGCCAAATGGAAATTTTGGAATGGGGCGGCGAAATTGACCAAGAAACTCGTTTGTTCGATCCAAATAAAATGGAAAC
ACGTTCTATGCGTTCTAAAGAAGAAGCGAATGACTACCGCTACTTCCCTGATCCAGACTTGTTACCAGTAATTATTCCAG
ATGAGCAAATTGAGGCCATCAAAGCAACTATGCCTGAGTTGCCAGCAGCACGCCGTGCACGCTTTATTGCAGACTTTGGC
GTAACTGAATACGACGCACACGTACTTACGCTCTCACGCGAAATGGCGGACTTCTATGAAGCTGTTGTTGCTGCGGCTGG
TGGTGCGAAGCAAGGTAAAATTGCTGCAAACTGGGTAATGGGTGAATTCTCTGGTGCTTTAAATAAAGCGGGCTTAGAGT
TAACAGACTCACCTGTTTCTGCTGAACAGCTTGGCGGCATGATTGCACGTATTGTTGACAACACCATTAGCGGTAAAATT
GCTAAACAAGTCTTTGGCTTTATGTGGGAATCGGAAGGTAAATCTGCCGATGACATTATTGCGGAAAAAGGCTTAAAGCA
AGAAACTGACACGGGTGCAATTGAAGCAATCATTAAAGAAGTACTTGCAGCCAATGAAAAAATGGTTGAAGAATACAAGT
CTGGTAAAGAAAAAGCCTTTAACGGTCTTGTTGGTCAAGTTATGAAAGCTTCTAAGGGTAAAGCTAACCCTGCACAAGTA
AATGAATTAATGAAAAAATTGATTGGTTAA

Upstream 100 bases:

>100_bases
ATTACTGGTCAGAATCACAATTGTTGTCGATTGTTCACCAATACCAACAAAACACTGACTGGCACACCAAACGTGCGGCA
ATTGCTGAGGAGAATGCATA

Downstream 100 bases:

>100_bases
TTTCATTAACTAACTCAATAAAAAAGCTTCGATTTATCGGGGCTTTTTTATACCACTTATGAAATTTCTTCGACTAAAGA
ATGCGCTTTTTTCTAAAAAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MAEAQKLKLIDGWEVVIGIEIHTQLATNSKIFSGSSTEFGQDPNTQASLVDLAMPGVLPVLNEKVVELAIRFGLGIDAYI
DQASVFARKNYFYPDSPKGYQISQMDNPIVGLGHIDIQLEDGTTKRIGVTRAHLEEDAGKSVHDQFEGMSGIDLNRAGTP
LLEIVSEPDMRSVEEAVAYIKAIHTLVRWLGISDGNMAEGSFRADCNVSLRRPGQPFGTRCELKNLNSFRFIEQAINVEI
ERQMEILEWGGEIDQETRLFDPNKMETRSMRSKEEANDYRYFPDPDLLPVIIPDEQIEAIKATMPELPAARRARFIADFG
VTEYDAHVLTLSREMADFYEAVVAAAGGAKQGKIAANWVMGEFSGALNKAGLELTDSPVSAEQLGGMIARIVDNTISGKI
AKQVFGFMWESEGKSADDIIAEKGLKQETDTGAIEAIIKEVLAANEKMVEEYKSGKEKAFNGLVGQVMKASKGKANPAQV
NELMKKLIG

Sequences:

>Translated_489_residues
MAEAQKLKLIDGWEVVIGIEIHTQLATNSKIFSGSSTEFGQDPNTQASLVDLAMPGVLPVLNEKVVELAIRFGLGIDAYI
DQASVFARKNYFYPDSPKGYQISQMDNPIVGLGHIDIQLEDGTTKRIGVTRAHLEEDAGKSVHDQFEGMSGIDLNRAGTP
LLEIVSEPDMRSVEEAVAYIKAIHTLVRWLGISDGNMAEGSFRADCNVSLRRPGQPFGTRCELKNLNSFRFIEQAINVEI
ERQMEILEWGGEIDQETRLFDPNKMETRSMRSKEEANDYRYFPDPDLLPVIIPDEQIEAIKATMPELPAARRARFIADFG
VTEYDAHVLTLSREMADFYEAVVAAAGGAKQGKIAANWVMGEFSGALNKAGLELTDSPVSAEQLGGMIARIVDNTISGKI
AKQVFGFMWESEGKSADDIIAEKGLKQETDTGAIEAIIKEVLAANEKMVEEYKSGKEKAFNGLVGQVMKASKGKANPAQV
NELMKKLIG
>Mature_488_residues
AEAQKLKLIDGWEVVIGIEIHTQLATNSKIFSGSSTEFGQDPNTQASLVDLAMPGVLPVLNEKVVELAIRFGLGIDAYID
QASVFARKNYFYPDSPKGYQISQMDNPIVGLGHIDIQLEDGTTKRIGVTRAHLEEDAGKSVHDQFEGMSGIDLNRAGTPL
LEIVSEPDMRSVEEAVAYIKAIHTLVRWLGISDGNMAEGSFRADCNVSLRRPGQPFGTRCELKNLNSFRFIEQAINVEIE
RQMEILEWGGEIDQETRLFDPNKMETRSMRSKEEANDYRYFPDPDLLPVIIPDEQIEAIKATMPELPAARRARFIADFGV
TEYDAHVLTLSREMADFYEAVVAAAGGAKQGKIAANWVMGEFSGALNKAGLELTDSPVSAEQLGGMIARIVDNTISGKIA
KQVFGFMWESEGKSADDIIAEKGLKQETDTGAIEAIIKEVLAANEKMVEEYKSGKEKAFNGLVGQVMKASKGKANPAQVN
ELMKKLIG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=38.989898989899, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI115534094, Length=476, Percent_Identity=30.8823529411765, Blast_Score=199, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6319391, Length=520, Percent_Identity=31.1538461538462, Blast_Score=228, Evalue=2e-60,
Organism=Drosophila melanogaster, GI45550808, Length=490, Percent_Identity=33.0612244897959, Blast_Score=294, Evalue=9e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53725; Mature: 53594

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAQKLKLIDGWEVVIGIEIHTQLATNSKIFSGSSTEFGQDPNTQASLVDLAMPGVLPV
CCCCCEEEEECCEEEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCHHHHHHHCCCHHHH
LNEKVVELAIRFGLGIDAYIDQASVFARKNYFYPDSPKGYQISQMDNPIVGLGHIDIQLE
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCEEEEEEEEEEEC
DGTTKRIGVTRAHLEEDAGKSVHDQFEGMSGIDLNRAGTPLLEIVSEPDMRSVEEAVAYI
CCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHH
KAIHTLVRWLGISDGNMAEGSFRADCNVSLRRPGQPFGTRCELKNLNSFRFIEQAINVEI
HHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEECCCCCHHHHHHHHHCCHH
ERQMEILEWGGEIDQETRLFDPNKMETRSMRSKEEANDYRYFPDPDLLPVIIPDEQIEAI
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHH
KATMPELPAARRARFIADFGVTEYDAHVLTLSREMADFYEAVVAAAGGAKQGKIAANWVM
HHHCCCCCHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHH
GEFSGALNKAGLELTDSPVSAEQLGGMIARIVDNTISGKIAKQVFGFMWESEGKSADDII
HHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
AEKGLKQETDTGAIEAIIKEVLAANEKMVEEYKSGKEKAFNGLVGQVMKASKGKANPAQV
HHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHH
NELMKKLIG
HHHHHHHCC
>Mature Secondary Structure 
AEAQKLKLIDGWEVVIGIEIHTQLATNSKIFSGSSTEFGQDPNTQASLVDLAMPGVLPV
CCCCEEEEECCEEEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCHHHHHHHCCCHHHH
LNEKVVELAIRFGLGIDAYIDQASVFARKNYFYPDSPKGYQISQMDNPIVGLGHIDIQLE
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCEEEEEEEEEEEC
DGTTKRIGVTRAHLEEDAGKSVHDQFEGMSGIDLNRAGTPLLEIVSEPDMRSVEEAVAYI
CCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHH
KAIHTLVRWLGISDGNMAEGSFRADCNVSLRRPGQPFGTRCELKNLNSFRFIEQAINVEI
HHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEECCCCCHHHHHHHHHCCHH
ERQMEILEWGGEIDQETRLFDPNKMETRSMRSKEEANDYRYFPDPDLLPVIIPDEQIEAI
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHH
KATMPELPAARRARFIADFGVTEYDAHVLTLSREMADFYEAVVAAAGGAKQGKIAANWVM
HHHCCCCCHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHH
GEFSGALNKAGLELTDSPVSAEQLGGMIARIVDNTISGKIAKQVFGFMWESEGKSADDII
HHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
AEKGLKQETDTGAIEAIIKEVLAANEKMVEEYKSGKEKAFNGLVGQVMKASKGKANPAQV
HHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHH
NELMKKLIG
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA