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The map label for this gene is pfpI [H]

Identifier: 213157804

GI number: 213157804

Start: 3386535

End: 3387113

Strand: Direct

Name: pfpI [H]

Synonym: AB57_3287

Alternate gene names: 213157804

Gene position: 3386535-3387113 (Clockwise)

Preceding gene: 213157803

Following gene: 213157807

Centisome position: 83.61

GC content: 43.01

Gene sequence:

>579_bases
ATGTCTAAAAAGATTCTAATGTTGGTCGGTGACTATGCTGAAGACTATGAAACTATGGTTCCTTTCCAGTTCTTAACTGG
TTTAGGTTATACCGTGCATGCTGTTTGCCCAAATAAAAAAAACGGCGACCATATTGCGACGGCTATTCATGATTTTGAAG
GTGAGCAAACTTATAGCGAAAAACGTGGCCACAACTTCACAATTAACTATGATTTTGATGCCGTTAATACCGAAGACTAT
GTAGGTCTAGTCATTCCGGGTGGTCGTGCACCAGAGTATTTACGCATGAATGAACGTGTTGTCGAAATTGTACGTGATTT
TGATAGAGTGAAAAAACCAATTGCAGCCGTATGCCACGGTGCTCAGTTACTCGCTGCAGCAGACATTTTAAAAGACCGCC
TCTGCTCGGCTTATCCAGCATGTGCAGCCGAAGTAAAATTAGCAGGTGGGCAATATGCAGACATTGCTGTAACTGATGCC
GTTACAGATGGTCATTTGGTGACTGCTCCGGCTTGGCCTGCACATCCAGCTTGGTTAGCACAATTTGTGAAAGTTTTAGG
CGCTAAGATTAGTATCTAA

Upstream 100 bases:

>100_bases
TCGACTAAAGAATGCGCTTTTTTCTAAAAAATGACATACCGACTATGCTAATTTAAATTAAGAATTTAGATAAATTACAT
TTTAAAAAGAGGGATAAATC

Downstream 100 bases:

>100_bases
ATAAGATTCTTCACCCCAAATACAATAAAGCCTGCATTTAGATAGCAGGCTTTATTACTAAAATAGAAAACTAGATTTTT
ATCTTGTAATTCCCAACTTA

Product: intracellular protease, PfpI family

Products: NA

Alternate protein names: Intracellular protease I [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MSKKILMLVGDYAEDYETMVPFQFLTGLGYTVHAVCPNKKNGDHIATAIHDFEGEQTYSEKRGHNFTINYDFDAVNTEDY
VGLVIPGGRAPEYLRMNERVVEIVRDFDRVKKPIAAVCHGAQLLAAADILKDRLCSAYPACAAEVKLAGGQYADIAVTDA
VTDGHLVTAPAWPAHPAWLAQFVKVLGAKISI

Sequences:

>Translated_192_residues
MSKKILMLVGDYAEDYETMVPFQFLTGLGYTVHAVCPNKKNGDHIATAIHDFEGEQTYSEKRGHNFTINYDFDAVNTEDY
VGLVIPGGRAPEYLRMNERVVEIVRDFDRVKKPIAAVCHGAQLLAAADILKDRLCSAYPACAAEVKLAGGQYADIAVTDA
VTDGHLVTAPAWPAHPAWLAQFVKVLGAKISI
>Mature_191_residues
SKKILMLVGDYAEDYETMVPFQFLTGLGYTVHAVCPNKKNGDHIATAIHDFEGEQTYSEKRGHNFTINYDFDAVNTEDYV
GLVIPGGRAPEYLRMNERVVEIVRDFDRVKKPIAAVCHGAQLLAAADILKDRLCSAYPACAAEVKLAGGQYADIAVTDAV
TDGHLVTAPAWPAHPAWLAQFVKVLGAKISI

Specific function: Highly stable protease. Although the physiological function is unclear it is the dominant protease in P.furiosus [H]

COG id: COG0693

COG function: function code R; Putative intracellular protease/amidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PfpI endopeptidase domain [H]

Homologues:

Organism=Escherichia coli, GI87082219, Length=189, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006286
- InterPro:   IPR002818 [H]

Pfam domain/function: PF01965 DJ-1_PfpI [H]

EC number: NA

Molecular weight: Translated: 21011; Mature: 20880

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKILMLVGDYAEDYETMVPFQFLTGLGYTVHAVCPNKKNGDHIATAIHDFEGEQTYSE
CCCEEEEEECCCCHHHHHHCHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCHHHHH
KRGHNFTINYDFDAVNTEDYVGLVIPGGRAPEYLRMNERVVEIVRDFDRVKKPIAAVCHG
HCCCEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQLLAAADILKDRLCSAYPACAAEVKLAGGQYADIAVTDAVTDGHLVTAPAWPAHPAWLA
HHHHHHHHHHHHHHHHCCCCHHHEEEECCCCEEEEEEECCCCCCEEEECCCCCCCHHHHH
QFVKVLGAKISI
HHHHHHCCCCCC
>Mature Secondary Structure 
SKKILMLVGDYAEDYETMVPFQFLTGLGYTVHAVCPNKKNGDHIATAIHDFEGEQTYSE
CCEEEEEECCCCHHHHHHCHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCHHHHH
KRGHNFTINYDFDAVNTEDYVGLVIPGGRAPEYLRMNERVVEIVRDFDRVKKPIAAVCHG
HCCCEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQLLAAADILKDRLCSAYPACAAEVKLAGGQYADIAVTDAVTDGHLVTAPAWPAHPAWLA
HHHHHHHHHHHHHHHHCCCCHHHEEEECCCCEEEEEEECCCCCCEEEECCCCCCCHHHHH
QFVKVLGAKISI
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626329 [H]