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The map label for this gene is wax-dgaT [H]

Identifier: 213157794

GI number: 213157794

Start: 3376680

End: 3378065

Strand: Direct

Name: wax-dgaT [H]

Synonym: AB57_3277

Alternate gene names: 213157794

Gene position: 3376680-3378065 (Clockwise)

Preceding gene: 213157793

Following gene: 213157801

Centisome position: 83.36

GC content: 40.48

Gene sequence:

>1386_bases
ATGCGTCCATTACACCCTATTGATTTTATTTTTCTATCACTAGAAAAAAGACAACAACCGATGCATGTTGGCGGATTGTT
TTTATTTGAAATACCTGAAAATGCCCCTGAAACTTTCGTTCATGATTTAGTAGAAGAAATTCGCCAATCTAAAAGTATTC
CTGTTCCACCCTTCAATAATCGTTTAAATGGTCTATTTTGGGATGAAGATGAAGAGTTCGATATCGACCACCATTTTCGC
CATATTGCCTTGCCTCATCCTGGCCGGATTCGTGAATTACTGGTTTATATTTCCCAGCAGCACAGCTCTTTAATTGATCG
TGCAAAACCACTTTGGACGTGTGACATTATTGAAGGGATCGAAGGCAATCGTTTCGCAATGTACTTTAAGATTCATCATG
CCATGGTTGATGGTGTTGCAGGCATGCGCTTAATTGAGAAATCGCTGTCAAAAACACCACAAGAAAAACATGTGGTTCCA
CTTTGGTGTGTAGAAAGTAAAAGAACAAAACGCCTAAAAGTACCAAAACCAAGCACAAGTAAGATCAAAAGTATTTTAGG
TGGTATCAAATCACAGCTTGAAGTGACCCCTAAAGTTATGCAGGAATTATCGCAAACCATTTTTAAAGAAATGGGTAAAA
ATCCTGATTACGTCTCCACCTTTCAAGCTCCGGTTTCAATTTTAAATCAACGTGTAAGTGCTTCACGTCGGTTTGCAGCC
CAATCTTTTGAATTAAGCCGTCTTAGAAAAATTTCTAAAGCACTTGGTGTAACAATCAATGACGTCGTACTTGCTGTGTG
TTCTGGGGCATTACGTGAATATCTGATTAGTCACAACAGCCTGCCTAAAAAGCCTCTCATTGCCATGGTTCCTGCTTCCC
TACGCACAGATGACTCTGACGTGAGCAATCGTATTACCATGATTTTGGCAAATTTAGGAACCCATAAAGATCAGCCTCTA
GAGCGGCTCGAGATCATTCGTCGTAGTATGCAAAATTCAAAGCAACGCTTTAGCCGCATGACCGCGAATGAGATTTTAAA
CTATAGCGCTGTTGTTTATGGTCCAGCTGGACTTAACATTATGTCTGGGATGCTTCCTAAGCGTCAGGCATTCAATTTAG
TGATTTCCAATGTACCTGGCCCACGTGAGCCGCTTTATTGGAATGGTGCGAAACTAGATGCACTCTACCCTGCTTCTATT
GTCATGGACGGACAAGCGCTCAATATTACAATGACAAGCTATCTGGATAAGCTTGAAGTAGGTTTAACAGCATGTCGAAA
TGCTTTGCCTAAAATGCAAAACCTTTTAACGCATCTTGAAGATGAGATTCAACGGTTCGAAGAAATTATTGAAGAGAAAC
AGCTGAAACATCATTCAGCGAGTTAG

Upstream 100 bases:

>100_bases
TAATCTGTAATAAACGCTATATACTAAATTTACGGTTCTATAATTCTAATAAGAGCTCAGTTAAGGGCTTCTTAGAAAAA
AGCATGAGGCACAACGAGTT

Downstream 100 bases:

>100_bases
GAAAAATTAAGGGGCTTTAAAATGGCCCCTTATTTTTTAATTGCGCGGCACTGGTTAAGCAACTGATGACATACTGAACG
AATCATTGCAGTTGTGATTG

Product: hypothetical protein

Products: NA

Alternate protein names: Diacylglycerol O-acyltransferase; DGAT; Long-chain-alcohol O-fatty-acyltransferase; Wax ester synthase/acyl-CoA:diacylglycerol acyltransferase; Wax synthase; WS [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MRPLHPIDFIFLSLEKRQQPMHVGGLFLFEIPENAPETFVHDLVEEIRQSKSIPVPPFNNRLNGLFWDEDEEFDIDHHFR
HIALPHPGRIRELLVYISQQHSSLIDRAKPLWTCDIIEGIEGNRFAMYFKIHHAMVDGVAGMRLIEKSLSKTPQEKHVVP
LWCVESKRTKRLKVPKPSTSKIKSILGGIKSQLEVTPKVMQELSQTIFKEMGKNPDYVSTFQAPVSILNQRVSASRRFAA
QSFELSRLRKISKALGVTINDVVLAVCSGALREYLISHNSLPKKPLIAMVPASLRTDDSDVSNRITMILANLGTHKDQPL
ERLEIIRRSMQNSKQRFSRMTANEILNYSAVVYGPAGLNIMSGMLPKRQAFNLVISNVPGPREPLYWNGAKLDALYPASI
VMDGQALNITMTSYLDKLEVGLTACRNALPKMQNLLTHLEDEIQRFEEIIEEKQLKHHSAS

Sequences:

>Translated_461_residues
MRPLHPIDFIFLSLEKRQQPMHVGGLFLFEIPENAPETFVHDLVEEIRQSKSIPVPPFNNRLNGLFWDEDEEFDIDHHFR
HIALPHPGRIRELLVYISQQHSSLIDRAKPLWTCDIIEGIEGNRFAMYFKIHHAMVDGVAGMRLIEKSLSKTPQEKHVVP
LWCVESKRTKRLKVPKPSTSKIKSILGGIKSQLEVTPKVMQELSQTIFKEMGKNPDYVSTFQAPVSILNQRVSASRRFAA
QSFELSRLRKISKALGVTINDVVLAVCSGALREYLISHNSLPKKPLIAMVPASLRTDDSDVSNRITMILANLGTHKDQPL
ERLEIIRRSMQNSKQRFSRMTANEILNYSAVVYGPAGLNIMSGMLPKRQAFNLVISNVPGPREPLYWNGAKLDALYPASI
VMDGQALNITMTSYLDKLEVGLTACRNALPKMQNLLTHLEDEIQRFEEIIEEKQLKHHSAS
>Mature_461_residues
MRPLHPIDFIFLSLEKRQQPMHVGGLFLFEIPENAPETFVHDLVEEIRQSKSIPVPPFNNRLNGLFWDEDEEFDIDHHFR
HIALPHPGRIRELLVYISQQHSSLIDRAKPLWTCDIIEGIEGNRFAMYFKIHHAMVDGVAGMRLIEKSLSKTPQEKHVVP
LWCVESKRTKRLKVPKPSTSKIKSILGGIKSQLEVTPKVMQELSQTIFKEMGKNPDYVSTFQAPVSILNQRVSASRRFAA
QSFELSRLRKISKALGVTINDVVLAVCSGALREYLISHNSLPKKPLIAMVPASLRTDDSDVSNRITMILANLGTHKDQPL
ERLEIIRRSMQNSKQRFSRMTANEILNYSAVVYGPAGLNIMSGMLPKRQAFNLVISNVPGPREPLYWNGAKLDALYPASI
VMDGQALNITMTSYLDKLEVGLTACRNALPKMQNLLTHLEDEIQRFEEIIEEKQLKHHSAS

Specific function: Bifunctional wax ester synthase/diacylglycerol acyltransferase (WS and DGAT). Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis. WS us

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR009721
- InterPro:   IPR004255 [H]

Pfam domain/function: PF06974 DUF1298; PF03007 UPF0089 [H]

EC number: =2.3.1.20; =2.3.1.75 [H]

Molecular weight: Translated: 52364; Mature: 52364

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPLHPIDFIFLSLEKRQQPMHVGGLFLFEIPENAPETFVHDLVEEIRQSKSIPVPPFNN
CCCCCCHHHHEEEHHHHCCCCEECCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
RLNGLFWDEDEEFDIDHHFRHIALPHPGRIRELLVYISQQHSSLIDRAKPLWTCDIIEGI
CCCCCCCCCCCCCCHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCC
EGNRFAMYFKIHHAMVDGVAGMRLIEKSLSKTPQEKHVVPLWCVESKRTKRLKVPKPSTS
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEECCCCCCHH
KIKSILGGIKSQLEVTPKVMQELSQTIFKEMGKNPDYVSTFQAPVSILNQRVSASRRFAA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QSFELSRLRKISKALGVTINDVVLAVCSGALREYLISHNSLPKKPLIAMVPASLRTDDSD
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHH
VSNRITMILANLGTHKDQPLERLEIIRRSMQNSKQRFSRMTANEILNYSAVVYGPAGLNI
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHH
MSGMLPKRQAFNLVISNVPGPREPLYWNGAKLDALYPASIVMDGQALNITMTSYLDKLEV
HHCCCCCHHHHHHHEECCCCCCCCCEECCCEECEECCCCEEECCCEEEEEHHHHHHHHHH
GLTACRNALPKMQNLLTHLEDEIQRFEEIIEEKQLKHHSAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRPLHPIDFIFLSLEKRQQPMHVGGLFLFEIPENAPETFVHDLVEEIRQSKSIPVPPFNN
CCCCCCHHHHEEEHHHHCCCCEECCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
RLNGLFWDEDEEFDIDHHFRHIALPHPGRIRELLVYISQQHSSLIDRAKPLWTCDIIEGI
CCCCCCCCCCCCCCHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCC
EGNRFAMYFKIHHAMVDGVAGMRLIEKSLSKTPQEKHVVPLWCVESKRTKRLKVPKPSTS
CCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEECCCCCCHH
KIKSILGGIKSQLEVTPKVMQELSQTIFKEMGKNPDYVSTFQAPVSILNQRVSASRRFAA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QSFELSRLRKISKALGVTINDVVLAVCSGALREYLISHNSLPKKPLIAMVPASLRTDDSD
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHH
VSNRITMILANLGTHKDQPLERLEIIRRSMQNSKQRFSRMTANEILNYSAVVYGPAGLNI
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHH
MSGMLPKRQAFNLVISNVPGPREPLYWNGAKLDALYPASIVMDGQALNITMTSYLDKLEV
HHCCCCCHHHHHHHEECCCCCCCCCEECCCEECEECCCCEEECCCEEEEEHHHHHHHHHH
GLTACRNALPKMQNLLTHLEDEIQRFEEIIEEKQLKHHSAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12502715 [H]