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The map label for this gene is pldA [C]

Identifier: 213157563

GI number: 213157563

Start: 2339921

End: 2341072

Strand: Reverse

Name: pldA [C]

Synonym: AB57_2255

Alternate gene names: 213157563

Gene position: 2341072-2339921 (Counterclockwise)

Preceding gene: 213157564

Following gene: 213157559

Centisome position: 57.8

GC content: 40.62

Gene sequence:

>1152_bases
ATGGCGTTCAGACAATTTGAACGCACATCTTTACAGCTAAGTATGGGAACCCTGCTTAGCTGTTTATGTGCGTCGGTGGC
ATATGCCGACACCTTAGCCCCTGTAAAACCTGCGAGTGTAGATGCATGTGTTGCCCTTGCTTCGAATGCTGATCGCCTAG
CTTGTTATGATGCTGTGTTTAAACCATCGGCCTTACCGGTTGTTCAAGCCGCAGTAGTACCAGAGCCTGTTAAAAAAATT
GATAAACCTGTTGTTCAACCTGAAACATTTAAAGAAAAGGTTGTAGATAAGGTAAGTAATATCAAAGTAATTGGTAAAGC
ACCTACAATTGAACCTACTTCTTCTTTGCTAGATCAACGTTGGGAGTTGTCTGAAAAAAGCAAACTGGGTGTTTGGAATA
TTCGTGCTTATCAACCTGTTTACTTGCTACCAGTTTTCTGGACGAGCGATAAAAATGAGTTTCCAAGCAGTCCAAACCCA
AATAATACGGTGACAGAAGCCCAAAACCTTAAATCAACTGAAAGTAAATTCCAGATTTCTTTGAAAACGAAAGCTTGGGA
AAATATTTTTGGTAACAATGGTGACTTGTGGGTAGGTTATACTCAATCTTCACGTTGGCAGACCTTTAATGCTGAAGAAT
CTCGTCCATTCCGTGAAACGAACTATGAGCCAGAAGCAAGTTTAATGTTTAGAACAAACTATGAGTTGCTTGGCTTAGAT
GGTCGTTTGCTTGGGGTAACTTTAAATCACCAGTCAAATGGACGTTCAGATCCTTTATCTCGTAGCTGGAACCGTGTCAT
CTTTAATGTGGGTTTAGAACGCGGTAACTTTGCTTTAATGCTTCGTCCATGGATACGCATTGAAGAAGACAGCAAAGATG
ATAATAATCCAGATATGGAAGATTACATCGGTCGTGGTGATTTAACTGCTTTTTATAAGTGGAAGCAAAATGACTTCTCA
TTAATGCTACGTCACTCTTTAAAAGGTGGTGATGATTCTCATGGTGCTGTTCAGTTTGACTGGGCTTTCCCGATTAGTGG
CAAGCTGCGCGGACACTTCCAATTATTTAATGGTTATGGTGAAAGCTTAATTGACTATAACCATCGTGCAACTTATGCCG
GTTTGGGTGTTTCACTCATGAACTGGTACTAA

Upstream 100 bases:

>100_bases
CTGTAAAAGCTGTCGTTGCACCTTTTGCAAAACGTGATGAATTGCAACAACTGGGCTTTACGATCAAACAAGTTAATTAA
AATAAAAATTGGAGATGAAC

Downstream 100 bases:

>100_bases
GTTTGACCTATAAAAATGCCGCTTTTACAGCGGCTTTTTTATTAACCAATTTGAATATTGGCTTGAGGATGTTTGAGACG
GCTTTTTTTAGGAGTAGCAA

Product: phospholipase

Products: NA

Alternate protein names: Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MAFRQFERTSLQLSMGTLLSCLCASVAYADTLAPVKPASVDACVALASNADRLACYDAVFKPSALPVVQAAVVPEPVKKI
DKPVVQPETFKEKVVDKVSNIKVIGKAPTIEPTSSLLDQRWELSEKSKLGVWNIRAYQPVYLLPVFWTSDKNEFPSSPNP
NNTVTEAQNLKSTESKFQISLKTKAWENIFGNNGDLWVGYTQSSRWQTFNAEESRPFRETNYEPEASLMFRTNYELLGLD
GRLLGVTLNHQSNGRSDPLSRSWNRVIFNVGLERGNFALMLRPWIRIEEDSKDDNNPDMEDYIGRGDLTAFYKWKQNDFS
LMLRHSLKGGDDSHGAVQFDWAFPISGKLRGHFQLFNGYGESLIDYNHRATYAGLGVSLMNWY

Sequences:

>Translated_383_residues
MAFRQFERTSLQLSMGTLLSCLCASVAYADTLAPVKPASVDACVALASNADRLACYDAVFKPSALPVVQAAVVPEPVKKI
DKPVVQPETFKEKVVDKVSNIKVIGKAPTIEPTSSLLDQRWELSEKSKLGVWNIRAYQPVYLLPVFWTSDKNEFPSSPNP
NNTVTEAQNLKSTESKFQISLKTKAWENIFGNNGDLWVGYTQSSRWQTFNAEESRPFRETNYEPEASLMFRTNYELLGLD
GRLLGVTLNHQSNGRSDPLSRSWNRVIFNVGLERGNFALMLRPWIRIEEDSKDDNNPDMEDYIGRGDLTAFYKWKQNDFS
LMLRHSLKGGDDSHGAVQFDWAFPISGKLRGHFQLFNGYGESLIDYNHRATYAGLGVSLMNWY
>Mature_382_residues
AFRQFERTSLQLSMGTLLSCLCASVAYADTLAPVKPASVDACVALASNADRLACYDAVFKPSALPVVQAAVVPEPVKKID
KPVVQPETFKEKVVDKVSNIKVIGKAPTIEPTSSLLDQRWELSEKSKLGVWNIRAYQPVYLLPVFWTSDKNEFPSSPNPN
NTVTEAQNLKSTESKFQISLKTKAWENIFGNNGDLWVGYTQSSRWQTFNAEESRPFRETNYEPEASLMFRTNYELLGLDG
RLLGVTLNHQSNGRSDPLSRSWNRVIFNVGLERGNFALMLRPWIRIEEDSKDDNNPDMEDYIGRGDLTAFYKWKQNDFSL
MLRHSLKGGDDSHGAVQFDWAFPISGKLRGHFQLFNGYGESLIDYNHRATYAGLGVSLMNWY

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=213, Percent_Identity=41.3145539906103, Blast_Score=157, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 43276; Mature: 43145

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFRQFERTSLQLSMGTLLSCLCASVAYADTLAPVKPASVDACVALASNADRLACYDAVF
CCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEHHHHC
KPSALPVVQAAVVPEPVKKIDKPVVQPETFKEKVVDKVSNIKVIGKAPTIEPTSSLLDQR
CCCCCCHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHH
WELSEKSKLGVWNIRAYQPVYLLPVFWTSDKNEFPSSPNPNNTVTEAQNLKSTESKFQIS
HCCCCCCCCCEEEEEECCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEE
LKTKAWENIFGNNGDLWVGYTQSSRWQTFNAEESRPFRETNYEPEASLMFRTNYELLGLD
EECHHHHHHCCCCCCEEEEECCCCCEEECCCCCCCCCHHCCCCCCCEEEEEECCEEEECC
GRLLGVTLNHQSNGRSDPLSRSWNRVIFNVGLERGNFALMLRPWIRIEEDSKDDNNPDME
CEEEEEEEECCCCCCCCCCHHCCCEEEEEECCCCCCEEEEEECCEEEECCCCCCCCCCHH
DYIGRGDLTAFYKWKQNDFSLMLRHSLKGGDDSHGAVQFDWAFPISGKLRGHFQLFNGYG
HHCCCCCEEEEEEECCCCCEEEEEHHCCCCCCCCCEEEEEEECCCCCCEEEEEEEECCCC
ESLIDYNHRATYAGLGVSLMNWY
CHHHCCCCCEEEEECCEEEECCC
>Mature Secondary Structure 
AFRQFERTSLQLSMGTLLSCLCASVAYADTLAPVKPASVDACVALASNADRLACYDAVF
CCCCCCHHHEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEHHHHC
KPSALPVVQAAVVPEPVKKIDKPVVQPETFKEKVVDKVSNIKVIGKAPTIEPTSSLLDQR
CCCCCCHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHH
WELSEKSKLGVWNIRAYQPVYLLPVFWTSDKNEFPSSPNPNNTVTEAQNLKSTESKFQIS
HCCCCCCCCCEEEEEECCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEE
LKTKAWENIFGNNGDLWVGYTQSSRWQTFNAEESRPFRETNYEPEASLMFRTNYELLGLD
EECHHHHHHCCCCCCEEEEECCCCCEEECCCCCCCCCHHCCCCCCCEEEEEECCEEEECC
GRLLGVTLNHQSNGRSDPLSRSWNRVIFNVGLERGNFALMLRPWIRIEEDSKDDNNPDME
CEEEEEEEECCCCCCCCCCHHCCCEEEEEECCCCCCEEEEEECCEEEECCCCCCCCCCHH
DYIGRGDLTAFYKWKQNDFSLMLRHSLKGGDDSHGAVQFDWAFPISGKLRGHFQLFNGYG
HHCCCCCEEEEEEECCCCCEEEEEHHCCCCCCCCCEEEEEEECCCCCCEEEEEEEECCCC
ESLIDYNHRATYAGLGVSLMNWY
CHHHCCCCCEEEEECCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]