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The map label for this gene is pcaJ [H]

Identifier: 213157494

GI number: 213157494

Start: 2265114

End: 2265764

Strand: Direct

Name: pcaJ [H]

Synonym: AB57_2183

Alternate gene names: 213157494

Gene position: 2265114-2265764 (Clockwise)

Preceding gene: 213157493

Following gene: 213157495

Centisome position: 55.92

GC content: 48.54

Gene sequence:

>651_bases
ATGAGCTACCAGAAACTCAGCCGTGACCAGATTGCACAACGTGTGGCACAAGATATTCCCGATGGTGCCTATGTGAACTT
GGGTATTGGTTTACCAACCAAGATTGCCAGCTACTTACCTGCTGACAAAGATGTCTTCCTACATTCTGAAAATGGCCTGT
TGGCTTTTGGCCCACCGCCAGCGGCAGGTGAAGAAGACCCTGAACTGATTAATGCGGGTAAAGAGTTTGTGACCATGCTT
GAAGGCGGCAGTTTTTTCCACCATGGCGATTCATTTGCCATGATGCGTGGTGGTCACCTTGATATTGCGGTGCTTGGGGC
ATTTCAGGTGGCAGCGAATGGTGACTTAGCGAACTGGCACACGGGTGCGCCGGATGCGATTCCTGCCGTAGGTGGTGCGA
TGGATTTGGCGGTTGGTGCCAAGAAAGTATTTATCACTACAGACCATGTGACTAAACAAGGTGAGCCGAAGATTGTGGCT
GAGCTGACCTATCCGGTAACGGGTAAACACTGTGTTGACCGTATTTACACTGACCTGTGCGTGATTGATGTGACCAGAGA
TGGTTTAAAGGTGATTGAGAAAGTTGAAGGTCTTAGCTTTGATGAGTTACAGGCTTTAACCGGTGCAACTTTGATTGATG
CAACGAATTAA

Upstream 100 bases:

>100_bases
ACTCGGTGGATTAGACCCTGAGCACATTATCACCCCAGGTATTTTTGTACAGCATGTTGTACAAGTACAGCCCGCACAGT
AAGCAATAAGGAGAAATAAC

Downstream 100 bases:

>100_bases
GTCTCTAAATTGATTGAGTATGGCAGCTAATCAATAGCGCCACAGCCTATAAATGATGGTGATATTCAAAGGCCCTTTGT
ATAGGCAATACCTTACTTTT

Product: 3-oxoadipate CoA-transferase subunit B

Products: NA

Alternate protein names: Beta-ketoadipate:succinyl-CoA transferase subunit B [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MSYQKLSRDQIAQRVAQDIPDGAYVNLGIGLPTKIASYLPADKDVFLHSENGLLAFGPPPAAGEEDPELINAGKEFVTML
EGGSFFHHGDSFAMMRGGHLDIAVLGAFQVAANGDLANWHTGAPDAIPAVGGAMDLAVGAKKVFITTDHVTKQGEPKIVA
ELTYPVTGKHCVDRIYTDLCVIDVTRDGLKVIEKVEGLSFDELQALTGATLIDATN

Sequences:

>Translated_216_residues
MSYQKLSRDQIAQRVAQDIPDGAYVNLGIGLPTKIASYLPADKDVFLHSENGLLAFGPPPAAGEEDPELINAGKEFVTML
EGGSFFHHGDSFAMMRGGHLDIAVLGAFQVAANGDLANWHTGAPDAIPAVGGAMDLAVGAKKVFITTDHVTKQGEPKIVA
ELTYPVTGKHCVDRIYTDLCVIDVTRDGLKVIEKVEGLSFDELQALTGATLIDATN
>Mature_215_residues
SYQKLSRDQIAQRVAQDIPDGAYVNLGIGLPTKIASYLPADKDVFLHSENGLLAFGPPPAAGEEDPELINAGKEFVTMLE
GGSFFHHGDSFAMMRGGHLDIAVLGAFQVAANGDLANWHTGAPDAIPAVGGAMDLAVGAKKVFITTDHVTKQGEPKIVAE
LTYPVTGKHCVDRIYTDLCVIDVTRDGLKVIEKVEGLSFDELQALTGATLIDATN

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=204, Percent_Identity=48.0392156862745, Blast_Score=184, Evalue=6e-47,
Organism=Homo sapiens, GI11545841, Length=212, Percent_Identity=46.2264150943396, Blast_Score=184, Evalue=7e-47,
Organism=Escherichia coli, GI1788552, Length=203, Percent_Identity=41.3793103448276, Blast_Score=155, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17531517, Length=213, Percent_Identity=46.9483568075117, Blast_Score=182, Evalue=1e-46,
Organism=Drosophila melanogaster, GI21358325, Length=211, Percent_Identity=46.9194312796209, Blast_Score=185, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24655794, Length=211, Percent_Identity=46.9194312796209, Blast_Score=185, Evalue=2e-47,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.6 [H]

Molecular weight: Translated: 22932; Mature: 22801

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYQKLSRDQIAQRVAQDIPDGAYVNLGIGLPTKIASYLPADKDVFLHSENGLLAFGPPP
CCHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCCEEEECCCCEEEECCCC
AAGEEDPELINAGKEFVTMLEGGSFFHHGDSFAMMRGGHLDIAVLGAFQVAANGDLANWH
CCCCCCHHHHHCCHHHHHHHCCCCEEECCCCEEEEECCCEEEEEEEEEEEECCCCCCCCC
TGAPDAIPAVGGAMDLAVGAKKVFITTDHVTKQGEPKIVAELTYPVTGKHCVDRIYTDLC
CCCCCCHHHCCCHHHHHCCCEEEEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHEE
VIDVTRDGLKVIEKVEGLSFDELQALTGATLIDATN
EEEECCHHHHHHHHHCCCCHHHHHHHHCCEEEECCC
>Mature Secondary Structure 
SYQKLSRDQIAQRVAQDIPDGAYVNLGIGLPTKIASYLPADKDVFLHSENGLLAFGPPP
CHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCCEEEECCCCEEEECCCC
AAGEEDPELINAGKEFVTMLEGGSFFHHGDSFAMMRGGHLDIAVLGAFQVAANGDLANWH
CCCCCCHHHHHCCHHHHHHHCCCCEEECCCCEEEEECCCEEEEEEEEEEEECCCCCCCCC
TGAPDAIPAVGGAMDLAVGAKKVFITTDHVTKQGEPKIVAELTYPVTGKHCVDRIYTDLC
CCCCCCHHHCCCHHHHHCCCEEEEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHEE
VIDVTRDGLKVIEKVEGLSFDELQALTGATLIDATN
EEEECCHHHHHHHHHCCCCHHHHHHHHCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8063101; 8125318 [H]