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The map label for this gene is ilvD [H]

Identifier: 213157486

GI number: 213157486

Start: 2257296

End: 2259029

Strand: Direct

Name: ilvD [H]

Synonym: AB57_2175

Alternate gene names: 213157486

Gene position: 2257296-2259029 (Clockwise)

Preceding gene: 213157482

Following gene: 213157487

Centisome position: 55.73

GC content: 44.46

Gene sequence:

>1734_bases
ATGAATAACAAACAAGTTCTTCGTTCGGCTGCCTGGTTTGGTACAACGGATAAAAATGGCTTTATGTATCGCAGCTGGAT
GAAGAACCAAGGAATTCCAGATCATGAGTTTCAAGGCAAGCCCATTATTGGAATTTGTAATACGTGGTCTGAACTCACCC
CATGTAATGCGCATTTTCGAAAAATTGCCGAACACGTAAAAAAAGGGATTTTAGAGGCGGGCGGCTACCCTGTTGAGTTT
CCTGTATTTTCAAATGGTGAGTCGAATTTACGTCCTACCGCCATGTTTACCCGCAACTTAGCCAGTATGGATGTCGAAGA
AGCGATTCGTGGTAACCCTATTGACGGTGTGGTGTTACTGACAGGTTGTGACAAAACCACCCCTGCATTATTAATGGGAG
CTGCAAGTTGTGATATTCCTGCCATTGTAGTGACTGGTGGACCAATGCTGAATGGTAAACACAAAGGTAAAGACATTGGT
GCAGGTACTATCGTTTGGCAAATGCATGAAGAGTTAAAAGCAGGCAAAATTGATTTAAATGAATTTCTATCTGCCGAATC
TGGTATGTCACGCTCGGCTGGTACATGTAATACCATGGGCACTGCTTCTACAATGGCCTGTATGGCTGAAGCCCTAGGCA
CTTCCTTACCCCACAATGCGGCCATTCCAGCGGTAGATTCTCGTCGTTATGTGTTGGCTCATCTTTCTGGTATGCGTATT
GTTGATATGGTTCATGAAGATTTACGCCTTTCTAAAATCTTAACCAAAGAAGCCTTTGAAAATGCGATTAAAGTCAATGC
AGCTATTGGTGGTTCAACCAACGCCGTGATTCATTTAAAAGCAATTGCTGGACGCATTGGGGTTGATTTACAACTGGATG
ACTGGAACCGTGTTGGCCGCGGCATGCCAACCATTGTCGACTTACAGCCTTCGGGTCGTTTTTTAATGGAAGAGTTCTAT
TATAGCGGCGGCTTGCCAGCCGTGATTCGTCGCATGGGTGAAGCCAATCTCTTACCACATCCTCAAGCTTTAACCGTAAA
CGGTCAAACCATTTGGGAAAACTGCCAACAGTCACCTATTTATAATGATGAAGTGATTCGGAAAATAGATAATCCAATTC
GACAAGATGGCGGTATGTGTATTTTGCGCGGAAATTTAGCGCCTAAAGGTGCGGTGCTTAAACCATCTGCTGCCACACCG
GAGTTGATGAAACACCGTGGTCGTGCAGTCGTATTTGAAAACTTCGATGATTATAAATCCCGTATTAACGACCCAGATCT
AGATGTCGATGAAACTTGTATTCTTGTGATGAAAAATGCTGGCCCTAAAGGATATCCGGGCATGGCCGAGGTTGGAAATA
TGGGACTACCACCAAAAATTTTAGCCAAAGGCATTACCGACATGGTTCGTATTTCCGACGCACGTATGAGTGGAACTGCG
TATGGCACGGTCGTTTTACATGTTGCCCCTGAAGCTATGGCGGGTGGGCCTTTGGCCGTAGTTCAAAATGGTGACTTCAT
TGAACTTGATGCCTATGCTGGAAAACTTCACCTAGAAGTGAGTGATGAAGAACTTAAGCAACGTCTTGAAAATTTAGCAC
CACCAGCTCCACCAAGTTTTATTGGCGGTTACCGTAAGCTCTATGTAGAACATGTTTTACAAGCAGACGAAGGTTGTGAT
TTTGATTTCTTGGTCGGTTGCCGTGGCTCAGAAGTTCCACGTCATTCCCATTAA

Upstream 100 bases:

>100_bases
ATAGTAATACTATTTAAATTTCAAACCTTTTAAAAATAAAATTTATGGTGTATTTTTAATTAAATTAATTCTAATAGTAA
TACTATATAGGGAAATTTGT

Downstream 100 bases:

>100_bases
TATTTATTCTTGAAAAAGATGATCTTGTTAAAACACAAGGTCATCTATACCATAGGATATTCAACATGTCGTAATAGTGA
AATATGAGTAAAGCTGACTA

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MNNKQVLRSAAWFGTTDKNGFMYRSWMKNQGIPDHEFQGKPIIGICNTWSELTPCNAHFRKIAEHVKKGILEAGGYPVEF
PVFSNGESNLRPTAMFTRNLASMDVEEAIRGNPIDGVVLLTGCDKTTPALLMGAASCDIPAIVVTGGPMLNGKHKGKDIG
AGTIVWQMHEELKAGKIDLNEFLSAESGMSRSAGTCNTMGTASTMACMAEALGTSLPHNAAIPAVDSRRYVLAHLSGMRI
VDMVHEDLRLSKILTKEAFENAIKVNAAIGGSTNAVIHLKAIAGRIGVDLQLDDWNRVGRGMPTIVDLQPSGRFLMEEFY
YSGGLPAVIRRMGEANLLPHPQALTVNGQTIWENCQQSPIYNDEVIRKIDNPIRQDGGMCILRGNLAPKGAVLKPSAATP
ELMKHRGRAVVFENFDDYKSRINDPDLDVDETCILVMKNAGPKGYPGMAEVGNMGLPPKILAKGITDMVRISDARMSGTA
YGTVVLHVAPEAMAGGPLAVVQNGDFIELDAYAGKLHLEVSDEELKQRLENLAPPAPPSFIGGYRKLYVEHVLQADEGCD
FDFLVGCRGSEVPRHSH

Sequences:

>Translated_577_residues
MNNKQVLRSAAWFGTTDKNGFMYRSWMKNQGIPDHEFQGKPIIGICNTWSELTPCNAHFRKIAEHVKKGILEAGGYPVEF
PVFSNGESNLRPTAMFTRNLASMDVEEAIRGNPIDGVVLLTGCDKTTPALLMGAASCDIPAIVVTGGPMLNGKHKGKDIG
AGTIVWQMHEELKAGKIDLNEFLSAESGMSRSAGTCNTMGTASTMACMAEALGTSLPHNAAIPAVDSRRYVLAHLSGMRI
VDMVHEDLRLSKILTKEAFENAIKVNAAIGGSTNAVIHLKAIAGRIGVDLQLDDWNRVGRGMPTIVDLQPSGRFLMEEFY
YSGGLPAVIRRMGEANLLPHPQALTVNGQTIWENCQQSPIYNDEVIRKIDNPIRQDGGMCILRGNLAPKGAVLKPSAATP
ELMKHRGRAVVFENFDDYKSRINDPDLDVDETCILVMKNAGPKGYPGMAEVGNMGLPPKILAKGITDMVRISDARMSGTA
YGTVVLHVAPEAMAGGPLAVVQNGDFIELDAYAGKLHLEVSDEELKQRLENLAPPAPPSFIGGYRKLYVEHVLQADEGCD
FDFLVGCRGSEVPRHSH
>Mature_577_residues
MNNKQVLRSAAWFGTTDKNGFMYRSWMKNQGIPDHEFQGKPIIGICNTWSELTPCNAHFRKIAEHVKKGILEAGGYPVEF
PVFSNGESNLRPTAMFTRNLASMDVEEAIRGNPIDGVVLLTGCDKTTPALLMGAASCDIPAIVVTGGPMLNGKHKGKDIG
AGTIVWQMHEELKAGKIDLNEFLSAESGMSRSAGTCNTMGTASTMACMAEALGTSLPHNAAIPAVDSRRYVLAHLSGMRI
VDMVHEDLRLSKILTKEAFENAIKVNAAIGGSTNAVIHLKAIAGRIGVDLQLDDWNRVGRGMPTIVDLQPSGRFLMEEFY
YSGGLPAVIRRMGEANLLPHPQALTVNGQTIWENCQQSPIYNDEVIRKIDNPIRQDGGMCILRGNLAPKGAVLKPSAATP
ELMKHRGRAVVFENFDDYKSRINDPDLDVDETCILVMKNAGPKGYPGMAEVGNMGLPPKILAKGITDMVRISDARMSGTA
YGTVVLHVAPEAMAGGPLAVVQNGDFIELDAYAGKLHLEVSDEELKQRLENLAPPAPPSFIGGYRKLYVEHVLQADEGCD
FDFLVGCRGSEVPRHSH

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=587, Percent_Identity=28.7904599659284, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1788157, Length=534, Percent_Identity=28.8389513108614, Blast_Score=185, Evalue=8e-48,
Organism=Escherichia coli, GI2367371, Length=483, Percent_Identity=32.5051759834368, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1786464, Length=445, Percent_Identity=31.2359550561798, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6322476, Length=575, Percent_Identity=31.304347826087, Blast_Score=262, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 62558; Mature: 62558

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00886 ILVD_EDD_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKQVLRSAAWFGTTDKNGFMYRSWMKNQGIPDHEFQGKPIIGICNTWSELTPCNAHFR
CCCHHHHHHHHHCCCCCCCCCEEHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHH
KIAEHVKKGILEAGGYPVEFPVFSNGESNLRPTAMFTRNLASMDVEEAIRGNPIDGVVLL
HHHHHHHHHHHHCCCCEEEECEECCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCCCEEEE
TGCDKTTPALLMGAASCDIPAIVVTGGPMLNGKHKGKDIGAGTIVWQMHEELKAGKIDLN
ECCCCCCHHHEEECCCCCCCEEEEECCCEECCCCCCCCCCCCEEEEEEHHHHCCCCCCHH
EFLSAESGMSRSAGTCNTMGTASTMACMAEALGTSLPHNAAIPAVDSRRYVLAHLSGMRI
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHCCCHH
VDMVHEDLRLSKILTKEAFENAIKVNAAIGGSTNAVIHLKAIAGRIGVDLQLDDWNRVGR
HHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEEEEEECCCCCEEEEECCHHHHCC
GMPTIVDLQPSGRFLMEEFYYSGGLPAVIRRMGEANLLPHPQALTVNGQTIWENCQQSPI
CCCEEEEECCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHCCCCC
YNDEVIRKIDNPIRQDGGMCILRGNLAPKGAVLKPSAATPELMKHRGRAVVFENFDDYKS
CCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCCCCCHHHHHCCCCEEEECCCHHHHH
RINDPDLDVDETCILVMKNAGPKGYPGMAEVGNMGLPPKILAKGITDMVRISDARMSGTA
HCCCCCCCCCCEEEEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCE
YGTVVLHVAPEAMAGGPLAVVQNGDFIELDAYAGKLHLEVSDEELKQRLENLAPPAPPSF
EEEEEEEECCHHHCCCCEEEEECCCEEEEEECCCEEEEEECHHHHHHHHHHCCCCCCHHH
IGGYRKLYVEHVLQADEGCDFDFLVGCRGSEVPRHSH
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MNNKQVLRSAAWFGTTDKNGFMYRSWMKNQGIPDHEFQGKPIIGICNTWSELTPCNAHFR
CCCHHHHHHHHHCCCCCCCCCEEHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHH
KIAEHVKKGILEAGGYPVEFPVFSNGESNLRPTAMFTRNLASMDVEEAIRGNPIDGVVLL
HHHHHHHHHHHHCCCCEEEECEECCCCCCCCCHHHHHHHHHHCCHHHHHCCCCCCCEEEE
TGCDKTTPALLMGAASCDIPAIVVTGGPMLNGKHKGKDIGAGTIVWQMHEELKAGKIDLN
ECCCCCCHHHEEECCCCCCCEEEEECCCEECCCCCCCCCCCCEEEEEEHHHHCCCCCCHH
EFLSAESGMSRSAGTCNTMGTASTMACMAEALGTSLPHNAAIPAVDSRRYVLAHLSGMRI
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHCCCHH
VDMVHEDLRLSKILTKEAFENAIKVNAAIGGSTNAVIHLKAIAGRIGVDLQLDDWNRVGR
HHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEEEEEECCCCCEEEEECCHHHHCC
GMPTIVDLQPSGRFLMEEFYYSGGLPAVIRRMGEANLLPHPQALTVNGQTIWENCQQSPI
CCCEEEEECCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHCCCCC
YNDEVIRKIDNPIRQDGGMCILRGNLAPKGAVLKPSAATPELMKHRGRAVVFENFDDYKS
CCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCCCCCHHHHHCCCCEEEECCCHHHHH
RINDPDLDVDETCILVMKNAGPKGYPGMAEVGNMGLPPKILAKGITDMVRISDARMSGTA
HCCCCCCCCCCEEEEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCE
YGTVVLHVAPEAMAGGPLAVVQNGDFIELDAYAGKLHLEVSDEELKQRLENLAPPAPPSF
EEEEEEEECCHHHCCCCEEEEECCCEEEEEECCCEEEEEECHHHHHHHHHHCCCCCCHHH
IGGYRKLYVEHVLQADEGCDFDFLVGCRGSEVPRHSH
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA