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The map label for this gene is yafC [C]

Identifier: 213157441

GI number: 213157441

Start: 2212425

End: 2213357

Strand: Direct

Name: yafC [C]

Synonym: AB57_2130

Alternate gene names: 213157441

Gene position: 2212425-2213357 (Clockwise)

Preceding gene: 213157438

Following gene: 213157443

Centisome position: 54.62

GC content: 40.51

Gene sequence:

>933_bases
ATGGATTTAACAAATTCGATTGATATCCGCACGCTTCGCTTCTTTATTAGTGTTTACAATGCTCAAAACTTCTCGAATGT
AGCACGTAAAGAAGATGTGTCTGCCTCCATGATTTCTCGAACCATTCAACAACTTGAAGATGCGTTGGGGCAACAACTTT
TCTACCGAAATACACGAGCAATCACACCGACCGAATCGGGTAAATTATTTTTTGAATATGCAAAAAGAATTACCGAACAA
TTTGATGAAGCTCAAAAGGCTTTACAAGATAAAACCATAGAGCCATCGGGACTCGTCCGCATTAATGCCCCTGTGTTTTT
TGGTCAACGGCATATTGCGCCGTGGTTGGCGGGTTTATCAGAGCGTTATCCAAGGCTTCTAATAGAACTAATTCAAACCG
ATGAATTTATAGACCCATTACGCGAAAGCACAGATTTAATATTTCGAATTGGAGCACTGACTGATTCATCATTTCATGCC
CGTATTTTTGGTACACAAAAATATCATTTAGCCGCCTCTCCTCAGTATGTTGCCAAATACGGTACGCTCGACCACCCAAC
AGAACTTAATCAACACAAGTGCCTAGTCTATAAAGGTTTTGAAGGTCCTAACCGCTGGTTAGTGAGAAAGCCCAATGAAG
AATGGGTACATTATCCAATTACCCCGTTACTTTCTTCAAACAATGCCGAGAGTTTAATGACAGCCGCGCTTCATGGCATG
GGGATTGTTTTATTTCCTGATTGGCTGATCGGCGAAGCTTTAAAAAAAGGTGACTTAATAAAGCTTTTACCTGAATATGA
AGTCGCGATTAAATCACAACCCCAGCATATCGCAGCCATTTATCCAAATGTTCGTCATCCATCACTTAATATTAGAGCAG
TGATAGATTATTTTGCAGAAATCTATGGCGACACACCGTATTGGCAGAATTAG

Upstream 100 bases:

>100_bases
TCTACATTGCCTCATCTGTATCTAGGCAACGAATACTAAGGTAGCCAATTCATCAGAAAAACAGCATAATTCATGAATCA
CTTTTGCAGAAATTGCACAA

Downstream 100 bases:

>100_bases
TTTTCTTTTCAATGCCAGCAAAGTATTCCAATATCGACATACTTTGCTGGTGTCTCATCAGAGATAAAAATAAGATTAAA
AACTTGAAATAAATAAGAAT

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MDLTNSIDIRTLRFFISVYNAQNFSNVARKEDVSASMISRTIQQLEDALGQQLFYRNTRAITPTESGKLFFEYAKRITEQ
FDEAQKALQDKTIEPSGLVRINAPVFFGQRHIAPWLAGLSERYPRLLIELIQTDEFIDPLRESTDLIFRIGALTDSSFHA
RIFGTQKYHLAASPQYVAKYGTLDHPTELNQHKCLVYKGFEGPNRWLVRKPNEEWVHYPITPLLSSNNAESLMTAALHGM
GIVLFPDWLIGEALKKGDLIKLLPEYEVAIKSQPQHIAAIYPNVRHPSLNIRAVIDYFAEIYGDTPYWQN

Sequences:

>Translated_310_residues
MDLTNSIDIRTLRFFISVYNAQNFSNVARKEDVSASMISRTIQQLEDALGQQLFYRNTRAITPTESGKLFFEYAKRITEQ
FDEAQKALQDKTIEPSGLVRINAPVFFGQRHIAPWLAGLSERYPRLLIELIQTDEFIDPLRESTDLIFRIGALTDSSFHA
RIFGTQKYHLAASPQYVAKYGTLDHPTELNQHKCLVYKGFEGPNRWLVRKPNEEWVHYPITPLLSSNNAESLMTAALHGM
GIVLFPDWLIGEALKKGDLIKLLPEYEVAIKSQPQHIAAIYPNVRHPSLNIRAVIDYFAEIYGDTPYWQN
>Mature_310_residues
MDLTNSIDIRTLRFFISVYNAQNFSNVARKEDVSASMISRTIQQLEDALGQQLFYRNTRAITPTESGKLFFEYAKRITEQ
FDEAQKALQDKTIEPSGLVRINAPVFFGQRHIAPWLAGLSERYPRLLIELIQTDEFIDPLRESTDLIFRIGALTDSSFHA
RIFGTQKYHLAASPQYVAKYGTLDHPTELNQHKCLVYKGFEGPNRWLVRKPNEEWVHYPITPLLSSNNAESLMTAALHGM
GIVLFPDWLIGEALKKGDLIKLLPEYEVAIKSQPQHIAAIYPNVRHPSLNIRAVIDYFAEIYGDTPYWQN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=298, Percent_Identity=29.8657718120805, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI87081978, Length=297, Percent_Identity=34.006734006734, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1789440, Length=290, Percent_Identity=28.6206896551724, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=29.0540540540541, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1789639, Length=306, Percent_Identity=28.4313725490196, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787128, Length=288, Percent_Identity=29.8611111111111, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787589, Length=263, Percent_Identity=25.8555133079848, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790783, Length=268, Percent_Identity=26.865671641791, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788706, Length=306, Percent_Identity=21.2418300653595, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI157672245, Length=121, Percent_Identity=30.5785123966942, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788297, Length=125, Percent_Identity=28, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790208, Length=128, Percent_Identity=31.25, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789173, Length=252, Percent_Identity=22.6190476190476, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787601, Length=146, Percent_Identity=23.972602739726, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786448, Length=264, Percent_Identity=23.8636363636364, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35532; Mature: 35532

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTNSIDIRTLRFFISVYNAQNFSNVARKEDVSASMISRTIQQLEDALGQQLFYRNTRA
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ITPTESGKLFFEYAKRITEQFDEAQKALQDKTIEPSGLVRINAPVFFGQRHIAPWLAGLS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEECCHHHHHHHHHHH
ERYPRLLIELIQTDEFIDPLRESTDLIFRIGALTDSSFHARIFGTQKYHLAASPQYVAKY
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCEEEEECCCHHHHHC
GTLDHPTELNQHKCLVYKGFEGPNRWLVRKPNEEWVHYPITPLLSSNNAESLMTAALHGM
CCCCCCCCCCCCEEEEEECCCCCCCEEEECCCCCEEEECCCHHCCCCCHHHHHHHHHHCC
GIVLFPDWLIGEALKKGDLIKLLPEYEVAIKSQPQHIAAIYPNVRHPSLNIRAVIDYFAE
EEEEECCHHHHHHHCCCCEEEECCCHHEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHH
IYGDTPYWQN
HHCCCCCCCC
>Mature Secondary Structure
MDLTNSIDIRTLRFFISVYNAQNFSNVARKEDVSASMISRTIQQLEDALGQQLFYRNTRA
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ITPTESGKLFFEYAKRITEQFDEAQKALQDKTIEPSGLVRINAPVFFGQRHIAPWLAGLS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEECCHHHHHHHHHHH
ERYPRLLIELIQTDEFIDPLRESTDLIFRIGALTDSSFHARIFGTQKYHLAASPQYVAKY
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCEEEEECCCHHHHHC
GTLDHPTELNQHKCLVYKGFEGPNRWLVRKPNEEWVHYPITPLLSSNNAESLMTAALHGM
CCCCCCCCCCCCEEEEEECCCCCCCEEEECCCCCEEEECCCHHCCCCCHHHHHHHHHHCC
GIVLFPDWLIGEALKKGDLIKLLPEYEVAIKSQPQHIAAIYPNVRHPSLNIRAVIDYFAE
EEEEECCHHHHHHHCCCCEEEECCCHHEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHH
IYGDTPYWQN
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]