Click here to switch to the map view.

The map label for this gene is lpdA [H]

Identifier: 213157244

GI number: 213157244

Start: 2018033

End: 2019436

Strand: Direct

Name: lpdA [H]

Synonym: AB57_1931

Alternate gene names: 213157244

Gene position: 2018033-2019436 (Clockwise)

Preceding gene: 213157243

Following gene: 213157245

Centisome position: 49.82

GC content: 42.81

Gene sequence:

>1404_bases
ATGTCAGAAGCTCAATTTGATTTAATTGTGATTGGCGCGGGACCGGGTGGGTATGTGGCTGCAATTCGTGCAGCCCAGCT
CGGGTTAAAAACCGCAATTGTAGAAGAGCGACATTTAGGCGGAATTTGTCTAAATTGGGGCTGTATTCCAACCAAAGCTT
TGCTTGCAGGGGCTGAGCTCGCAACCCAGTTTAAACATGCCAGACAGTTTGGTTTTCAGGTATCGCAACTCGATTTTGAT
ATTCAAAAGCTTGTGCAACATAGCCGACAAGTGTCAGCTCAATTAGTACAAGGCATCGATTACTTACTGAAAAAGAATCA
GGTGACGGTTTTTAATGGTCGTGCTCAGCTTACGGCAAAAGAAAAACTTGAAGTGACAGATGCTCAAGGTAATAGTCAGG
CTTTGAGTGCACCACATATCATTTTGGCAACTGGAGCTAAAGCTCGACATGTTCCTCAACTTCCTGTCGATGGAACATAT
GTCTGGAGCTATAAAGAAGCGCTTGTTCCAGAGCAGTTACCGAAAAGTTTGCTGGTTGTAGGTTCAGGTGCAATTGGTAG
CGAGTTTGCTAGCCTTTATCAAGACTTGGGTTGTCAGGTGACATTAATTGATTTAGCCAAGCAGATTTTACCAACTGAAG
ATGCGGAAGTTGCCCAATTTGTCAGAAAACAATTTGAACAAAAAGGTATGAAAGTCTTAACCGATGCTGTCGTTCAAAGT
ATTCAAATTGAAAATGAGCAAGTCCACTGTGTGGTTGAAACGGCAAATGATGTTCAAACGCTTGTTTTTGATCGCGTGCT
TTCGGCAATTGGTGTACAGCCGAATACCACAGGGCTAGGACTAGAACGTTTAGGTGTAGAACTCAATCCACAAGGTTTTG
TGGCAATTGATGACTATTGTAAAACCAACGTAGCAGGGCTTTATGCCATTGGTGATGTTGCTGGAGCACCTTGTCTCGCT
CATAAAGCAAGCCATGAAGCCATAATATGCGTCGAGAAAATCGCTGGTGTGGCAAACGTCCATTCACTCGACCGTAGCCA
GATACCTGGTTGTATTTTTACACATCCACAAGTGGCGAGTATTGGTTTAACGGAAAATGCAGCAAAAGCTAAGAATCTAC
CTATACGTATTGGTAAGTTTTCTTTAACTGCAAATGGTAAAGCTTTAGCAATTGGAGATGCGTCAGGTTTCGTTAAAACG
GTGGTACATGCAGAAACAGGTGAATTACTTGGTGCACATATGGTGGGTCATGAAGTGACCGAACATATCCAAGGATTTGT
CATTGCGAAATATCTAGAAGCAACTGATGAAAGTTTAGCTCAAGTGATTTTCCCGCATCCAACATTATCAGAAGCCATGC
ATGAATCCATTTTGGCTTCAATGCAACGAGCGATTCATATGTAG

Upstream 100 bases:

>100_bases
GATCACCGAGTTATTGATGGCGCAGTTGGGGCGAAATTCTTGGCCAGCTTTAAGCAGTTTGTTGAAAACCCTGCTTTAAT
TTTGGTGTAGGAGGACCCGC

Downstream 100 bases:

>100_bases
AAATTCTACATTTACAGGAGTTGAACATGGTTAAGGGATTACAAAATAAAGTCGCTTTAGTGACTGGGGCAGCCCAAGGC
ATTGGCCGTGGGATTGCCCT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSEAQFDLIVIGAGPGGYVAAIRAAQLGLKTAIVEERHLGGICLNWGCIPTKALLAGAELATQFKHARQFGFQVSQLDFD
IQKLVQHSRQVSAQLVQGIDYLLKKNQVTVFNGRAQLTAKEKLEVTDAQGNSQALSAPHIILATGAKARHVPQLPVDGTY
VWSYKEALVPEQLPKSLLVVGSGAIGSEFASLYQDLGCQVTLIDLAKQILPTEDAEVAQFVRKQFEQKGMKVLTDAVVQS
IQIENEQVHCVVETANDVQTLVFDRVLSAIGVQPNTTGLGLERLGVELNPQGFVAIDDYCKTNVAGLYAIGDVAGAPCLA
HKASHEAIICVEKIAGVANVHSLDRSQIPGCIFTHPQVASIGLTENAAKAKNLPIRIGKFSLTANGKALAIGDASGFVKT
VVHAETGELLGAHMVGHEVTEHIQGFVIAKYLEATDESLAQVIFPHPTLSEAMHESILASMQRAIHM

Sequences:

>Translated_467_residues
MSEAQFDLIVIGAGPGGYVAAIRAAQLGLKTAIVEERHLGGICLNWGCIPTKALLAGAELATQFKHARQFGFQVSQLDFD
IQKLVQHSRQVSAQLVQGIDYLLKKNQVTVFNGRAQLTAKEKLEVTDAQGNSQALSAPHIILATGAKARHVPQLPVDGTY
VWSYKEALVPEQLPKSLLVVGSGAIGSEFASLYQDLGCQVTLIDLAKQILPTEDAEVAQFVRKQFEQKGMKVLTDAVVQS
IQIENEQVHCVVETANDVQTLVFDRVLSAIGVQPNTTGLGLERLGVELNPQGFVAIDDYCKTNVAGLYAIGDVAGAPCLA
HKASHEAIICVEKIAGVANVHSLDRSQIPGCIFTHPQVASIGLTENAAKAKNLPIRIGKFSLTANGKALAIGDASGFVKT
VVHAETGELLGAHMVGHEVTEHIQGFVIAKYLEATDESLAQVIFPHPTLSEAMHESILASMQRAIHM
>Mature_466_residues
SEAQFDLIVIGAGPGGYVAAIRAAQLGLKTAIVEERHLGGICLNWGCIPTKALLAGAELATQFKHARQFGFQVSQLDFDI
QKLVQHSRQVSAQLVQGIDYLLKKNQVTVFNGRAQLTAKEKLEVTDAQGNSQALSAPHIILATGAKARHVPQLPVDGTYV
WSYKEALVPEQLPKSLLVVGSGAIGSEFASLYQDLGCQVTLIDLAKQILPTEDAEVAQFVRKQFEQKGMKVLTDAVVQSI
QIENEQVHCVVETANDVQTLVFDRVLSAIGVQPNTTGLGLERLGVELNPQGFVAIDDYCKTNVAGLYAIGDVAGAPCLAH
KASHEAIICVEKIAGVANVHSLDRSQIPGCIFTHPQVASIGLTENAAKAKNLPIRIGKFSLTANGKALAIGDASGFVKTV
VHAETGELLGAHMVGHEVTEHIQGFVIAKYLEATDESLAQVIFPHPTLSEAMHESILASMQRAIHM

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=462, Percent_Identity=36.3636363636364, Blast_Score=296, Evalue=3e-80,
Organism=Homo sapiens, GI50301238, Length=463, Percent_Identity=28.5097192224622, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI22035672, Length=452, Percent_Identity=28.5398230088496, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI291045266, Length=432, Percent_Identity=26.1574074074074, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI33519430, Length=468, Percent_Identity=24.3589743589744, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI33519428, Length=468, Percent_Identity=24.3589743589744, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI33519426, Length=468, Percent_Identity=24.3589743589744, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI148277065, Length=468, Percent_Identity=24.3589743589744, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI148277071, Length=468, Percent_Identity=24.3589743589744, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI291045268, Length=424, Percent_Identity=25.2358490566038, Blast_Score=105, Evalue=6e-23,
Organism=Escherichia coli, GI1786307, Length=454, Percent_Identity=33.4801762114537, Blast_Score=260, Evalue=2e-70,
Organism=Escherichia coli, GI87082354, Length=463, Percent_Identity=26.5658747300216, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1789915, Length=432, Percent_Identity=28.2407407407407, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI87081717, Length=465, Percent_Identity=27.0967741935484, Blast_Score=161, Evalue=9e-41,
Organism=Escherichia coli, GI1788892, Length=196, Percent_Identity=27.0408163265306, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI32565766, Length=462, Percent_Identity=37.012987012987, Blast_Score=288, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI17557007, Length=479, Percent_Identity=26.9311064718163, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71983429, Length=459, Percent_Identity=26.3616557734205, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=459, Percent_Identity=26.3616557734205, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=485, Percent_Identity=25.5670103092783, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17559934, Length=286, Percent_Identity=26.9230769230769, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321091, Length=473, Percent_Identity=36.1522198731501, Blast_Score=268, Evalue=9e-73,
Organism=Saccharomyces cerevisiae, GI6325240, Length=474, Percent_Identity=27.4261603375527, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6325166, Length=465, Percent_Identity=29.4623655913978, Blast_Score=173, Evalue=5e-44,
Organism=Drosophila melanogaster, GI21358499, Length=466, Percent_Identity=35.4077253218884, Blast_Score=271, Evalue=6e-73,
Organism=Drosophila melanogaster, GI24640553, Length=469, Percent_Identity=26.6524520255864, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24640549, Length=469, Percent_Identity=26.6524520255864, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24640551, Length=469, Percent_Identity=26.6524520255864, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17737741, Length=480, Percent_Identity=24.1666666666667, Blast_Score=105, Evalue=9e-23,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49962; Mature: 49831

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAQFDLIVIGAGPGGYVAAIRAAQLGLKTAIVEERHLGGICLNWGCIPTKALLAGAEL
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHH
ATQFKHARQFGFQVSQLDFDIQKLVQHSRQVSAQLVQGIDYLLKKNQVTVFNGRAQLTAK
HHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEC
EKLEVTDAQGNSQALSAPHIILATGAKARHVPQLPVDGTYVWSYKEALVPEQLPKSLLVV
CCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEHHHHHCHHHCCCEEEEE
GSGAIGSEFASLYQDLGCQVTLIDLAKQILPTEDAEVAQFVRKQFEQKGMKVLTDAVVQS
ECCCHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IQIENEQVHCVVETANDVQTLVFDRVLSAIGVQPNTTGLGLERLGVELNPQGFVAIDDYC
HEECCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCEECCCCEEEECCHH
KTNVAGLYAIGDVAGAPCLAHKASHEAIICVEKIAGVANVHSLDRSQIPGCIFTHPQVAS
CCCCCEEEEECCCCCCCHHHHCCCCCEEEEHHHHCCHHHHHHCHHHHCCCEEECCCCEEE
IGLTENAAKAKNLPIRIGKFSLTANGKALAIGDASGFVKTVVHAETGELLGAHMVGHEVT
ECCCCCCHHHCCCCEEEEEEEEECCCCEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHH
EHIQGFVIAKYLEATDESLAQVIFPHPTLSEAMHESILASMQRAIHM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEAQFDLIVIGAGPGGYVAAIRAAQLGLKTAIVEERHLGGICLNWGCIPTKALLAGAEL
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHH
ATQFKHARQFGFQVSQLDFDIQKLVQHSRQVSAQLVQGIDYLLKKNQVTVFNGRAQLTAK
HHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEC
EKLEVTDAQGNSQALSAPHIILATGAKARHVPQLPVDGTYVWSYKEALVPEQLPKSLLVV
CCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEHHHHHCHHHCCCEEEEE
GSGAIGSEFASLYQDLGCQVTLIDLAKQILPTEDAEVAQFVRKQFEQKGMKVLTDAVVQS
ECCCHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IQIENEQVHCVVETANDVQTLVFDRVLSAIGVQPNTTGLGLERLGVELNPQGFVAIDDYC
HEECCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCEECCCCEEEECCHH
KTNVAGLYAIGDVAGAPCLAHKASHEAIICVEKIAGVANVHSLDRSQIPGCIFTHPQVAS
CCCCCEEEEECCCCCCCHHHHCCCCCEEEEHHHHCCHHHHHHCHHHHCCCEEECCCCEEE
IGLTENAAKAKNLPIRIGKFSLTANGKALAIGDASGFVKTVVHAETGELLGAHMVGHEVT
ECCCCCCHHHCCCCEEEEEEEEECCCCEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHH
EHIQGFVIAKYLEATDESLAQVIFPHPTLSEAMHESILASMQRAIHM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]