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The map label for this gene is pdhC [H]

Identifier: 213157243

GI number: 213157243

Start: 2016532

End: 2018022

Strand: Direct

Name: pdhC [H]

Synonym: AB57_1930

Alternate gene names: 213157243

Gene position: 2016532-2018022 (Clockwise)

Preceding gene: 213157242

Following gene: 213157244

Centisome position: 49.78

GC content: 43.59

Gene sequence:

>1491_bases
ATGGAAGAAGGCACGATCGCCCAATGGTTGATTAAAGAGGGTGATAGCTTTAATAAGGGCGATGAAATTTGTGAAATTGA
AACCACAAAAATCGTCAATGTATTAGAAGCACCTTTTGCTGGAACGCTCCGTAAAATTTTAGCGAAAGATGGTGATACTT
TACCTGTTGGGGGTCTTATAGCCGTATGTGCTGATAGTGAAATTTCTAATGCAGAAATTGAAAAATTTATTGCATCACTA
GGTGGTTCAGCAGCTAAGGCCCCTGAAGCATCTTCGGAACAAAGTAAATCGGAAACATTTGCACCTGTTGCTGAAAAAGC
CGAACAACCACAGTCCGTAGCTGCAAGTGCTCCAGCTCCTACAAAGGTAGCGAAAGGTGACTACGCTGTACCAGAGTCAT
TACAAGGCTATCAAACATCTGATGAGTTGTTTGCAACGCCACATGCGTTAAAGCTTGCAGAAAAGCACAATGTGAACTTG
GCAAAAGTCAGGGGTTCTGGTCGTGAAGGACGTATTAGTGTTCAAGACATCCAGAAAGCTGTGCAGGCTGCTGGTGGTCA
GTGGCCTGATGTTAAGCAGCAAACTCAAGCTAAAGTGATTAAATCTACAGCCGATGATAGCCAAGTTTTAGCGACACCTG
TAGCACGTCGTTTAGCAAAACAATGGGGTATTAACCTAAATGACTGCCGTGTTTCAGGTACTCGTGGCCGTGTCTGCAAA
GAAGATGTTGAAGCAGTTTACTATCGTAACAATCCAACGTCGGTAAATGAACAATCTGCTCAATGTGCAGCTCAACCACA
ATCAACAATTACGACTGTGGCGATGAATGGGATGCGTAAAGTGATTGCTTCACGCTTACAAGCTGCAAAACGTAATGCAC
CGCATTTCCGCTTAGTGGTCGATCTCAACGTAGAGGCTTTGCAAAAATTACGTAAGCAGATTAATGAAACGGTTCCTCAA
GTCAAACTTTCTATTAATGACATGCTAATTAAAGCAGCTGCTGCGGCACTGATTAAAGTGCCTGAGGTGAATGTTCAATT
TGATGAGGCGACTCAATCAATTTTACAGTTCTCTCAAGCGGATATTTCGGTGGCCGTAGCGATTCCAAACGGTTTAATTA
CACCCATTGTAAAAGCAGCGAATCAGAAGTCTTTAGCACAAATTTCCGATGACATGCGTGATTTGGCAACGCGTGCTAAG
ACTGGCAAGTTACAACCTGACGAGTTTCAGGGAGGCAGCTTTAGCATTTCAAATTTAGGAATGTTAGGCATTAAACAGTT
CGATGCCATTATTAACCCACCGCAAGGTGCAATTATGGCATTAGGTGCTTCGGAATCTCGCGCTGTTGTCGAAAATGGCA
ATGTAGTGGTTCGTGAGATTGTCACAGCGACGTTGTCATGCGATCACCGAGTTATTGATGGCGCAGTTGGGGCGAAATTC
TTGGCCAGCTTTAAGCAGTTTGTTGAAAACCCTGCTTTAATTTTGGTGTAG

Upstream 100 bases:

>100_bases
ATTCCAAGTGTAGAGCGTATTGAGCAAGCTGTGCGAAAAACATTGGAGGCTTAGGATGAGCGAAATTAAAACGCTGGAGA
TCCCGAAATGGGGATTATCT

Downstream 100 bases:

>100_bases
GAGGACCCGCATGTCAGAAGCTCAATTTGATTTAATTGTGATTGGCGCGGGACCGGGTGGGTATGTGGCTGCAATTCGTG
CAGCCCAGCTCGGGTTAAAA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MEEGTIAQWLIKEGDSFNKGDEICEIETTKIVNVLEAPFAGTLRKILAKDGDTLPVGGLIAVCADSEISNAEIEKFIASL
GGSAAKAPEASSEQSKSETFAPVAEKAEQPQSVAASAPAPTKVAKGDYAVPESLQGYQTSDELFATPHALKLAEKHNVNL
AKVRGSGREGRISVQDIQKAVQAAGGQWPDVKQQTQAKVIKSTADDSQVLATPVARRLAKQWGINLNDCRVSGTRGRVCK
EDVEAVYYRNNPTSVNEQSAQCAAQPQSTITTVAMNGMRKVIASRLQAAKRNAPHFRLVVDLNVEALQKLRKQINETVPQ
VKLSINDMLIKAAAAALIKVPEVNVQFDEATQSILQFSQADISVAVAIPNGLITPIVKAANQKSLAQISDDMRDLATRAK
TGKLQPDEFQGGSFSISNLGMLGIKQFDAIINPPQGAIMALGASESRAVVENGNVVVREIVTATLSCDHRVIDGAVGAKF
LASFKQFVENPALILV

Sequences:

>Translated_496_residues
MEEGTIAQWLIKEGDSFNKGDEICEIETTKIVNVLEAPFAGTLRKILAKDGDTLPVGGLIAVCADSEISNAEIEKFIASL
GGSAAKAPEASSEQSKSETFAPVAEKAEQPQSVAASAPAPTKVAKGDYAVPESLQGYQTSDELFATPHALKLAEKHNVNL
AKVRGSGREGRISVQDIQKAVQAAGGQWPDVKQQTQAKVIKSTADDSQVLATPVARRLAKQWGINLNDCRVSGTRGRVCK
EDVEAVYYRNNPTSVNEQSAQCAAQPQSTITTVAMNGMRKVIASRLQAAKRNAPHFRLVVDLNVEALQKLRKQINETVPQ
VKLSINDMLIKAAAAALIKVPEVNVQFDEATQSILQFSQADISVAVAIPNGLITPIVKAANQKSLAQISDDMRDLATRAK
TGKLQPDEFQGGSFSISNLGMLGIKQFDAIINPPQGAIMALGASESRAVVENGNVVVREIVTATLSCDHRVIDGAVGAKF
LASFKQFVENPALILV
>Mature_496_residues
MEEGTIAQWLIKEGDSFNKGDEICEIETTKIVNVLEAPFAGTLRKILAKDGDTLPVGGLIAVCADSEISNAEIEKFIASL
GGSAAKAPEASSEQSKSETFAPVAEKAEQPQSVAASAPAPTKVAKGDYAVPESLQGYQTSDELFATPHALKLAEKHNVNL
AKVRGSGREGRISVQDIQKAVQAAGGQWPDVKQQTQAKVIKSTADDSQVLATPVARRLAKQWGINLNDCRVSGTRGRVCK
EDVEAVYYRNNPTSVNEQSAQCAAQPQSTITTVAMNGMRKVIASRLQAAKRNAPHFRLVVDLNVEALQKLRKQINETVPQ
VKLSINDMLIKAAAAALIKVPEVNVQFDEATQSILQFSQADISVAVAIPNGLITPIVKAANQKSLAQISDDMRDLATRAK
TGKLQPDEFQGGSFSISNLGMLGIKQFDAIINPPQGAIMALGASESRAVVENGNVVVREIVTATLSCDHRVIDGAVGAKF
LASFKQFVENPALILV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=296, Percent_Identity=41.8918918918919, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI203098753, Length=237, Percent_Identity=43.4599156118143, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI203098816, Length=230, Percent_Identity=43.9130434782609, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI260898739, Length=164, Percent_Identity=46.9512195121951, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI19923748, Length=225, Percent_Identity=33.7777777777778, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI110671329, Length=311, Percent_Identity=29.2604501607717, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1786305, Length=301, Percent_Identity=32.5581395348837, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1786946, Length=291, Percent_Identity=31.2714776632302, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17560088, Length=297, Percent_Identity=39.0572390572391, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17538894, Length=224, Percent_Identity=39.2857142857143, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17537937, Length=337, Percent_Identity=29.3768545994065, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=227, Percent_Identity=35.2422907488987, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324258, Length=323, Percent_Identity=35.9133126934984, Blast_Score=186, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6320352, Length=233, Percent_Identity=33.9055793991416, Blast_Score=133, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24582497, Length=290, Percent_Identity=37.5862068965517, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI20129315, Length=290, Percent_Identity=37.5862068965517, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI18859875, Length=303, Percent_Identity=32.3432343234323, Blast_Score=148, Evalue=9e-36,
Organism=Drosophila melanogaster, GI24645909, Length=226, Percent_Identity=33.6283185840708, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 52915; Mature: 52915

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEGTIAQWLIKEGDSFNKGDEICEIETTKIVNVLEAPFAGTLRKILAKDGDTLPVGGLI
CCCCHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEE
AVCADSEISNAEIEKFIASLGGSAAKAPEASSEQSKSETFAPVAEKAEQPQSVAASAPAP
EEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCC
TKVAKGDYAVPESLQGYQTSDELFATPHALKLAEKHNVNLAKVRGSGREGRISVQDIQKA
CCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHCCCEEEEEECCCCCCCEEHHHHHHH
VQAAGGQWPDVKQQTQAKVIKSTADDSQVLATPVARRLAKQWGINLNDCRVSGTRGRVCK
HHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHH
EDVEAVYYRNNPTSVNEQSAQCAAQPQSTITTVAMNGMRKVIASRLQAAKRNAPHFRLVV
HCCCEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
DLNVEALQKLRKQINETVPQVKLSINDMLIKAAAAALIKVPEVNVQFDEATQSILQFSQA
ECCHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHC
DISVAVAIPNGLITPIVKAANQKSLAQISDDMRDLATRAKTGKLQPDEFQGGSFSISNLG
CEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCC
MLGIKQFDAIINPPQGAIMALGASESRAVVENGNVVVREIVTATLSCDHRVIDGAVGAKF
HHHHHHHHHHCCCCCCCEEEECCCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHH
LASFKQFVENPALILV
HHHHHHHHCCCCEEEC
>Mature Secondary Structure
MEEGTIAQWLIKEGDSFNKGDEICEIETTKIVNVLEAPFAGTLRKILAKDGDTLPVGGLI
CCCCHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEE
AVCADSEISNAEIEKFIASLGGSAAKAPEASSEQSKSETFAPVAEKAEQPQSVAASAPAP
EEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCC
TKVAKGDYAVPESLQGYQTSDELFATPHALKLAEKHNVNLAKVRGSGREGRISVQDIQKA
CCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHCCCEEEEEECCCCCCCEEHHHHHHH
VQAAGGQWPDVKQQTQAKVIKSTADDSQVLATPVARRLAKQWGINLNDCRVSGTRGRVCK
HHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHH
EDVEAVYYRNNPTSVNEQSAQCAAQPQSTITTVAMNGMRKVIASRLQAAKRNAPHFRLVV
HCCCEEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
DLNVEALQKLRKQINETVPQVKLSINDMLIKAAAAALIKVPEVNVQFDEATQSILQFSQA
ECCHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHC
DISVAVAIPNGLITPIVKAANQKSLAQISDDMRDLATRAKTGKLQPDEFQGGSFSISNLG
CEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCC
MLGIKQFDAIINPPQGAIMALGASESRAVVENGNVVVREIVTATLSCDHRVIDGAVGAKF
HHHHHHHHHHCCCCCCCEEEECCCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHH
LASFKQFVENPALILV
HHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]