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The map label for this gene is ahpF [H]

Identifier: 213157013

GI number: 213157013

Start: 1778265

End: 1779830

Strand: Direct

Name: ahpF [H]

Synonym: AB57_1691

Alternate gene names: 213157013

Gene position: 1778265-1779830 (Clockwise)

Preceding gene: 213157012

Following gene: 213157022

Centisome position: 43.9

GC content: 43.55

Gene sequence:

>1566_bases
ATGTTAGATCAAAACATTAAAACTCAATTAAAAGCTTACCTAGAACGTTTAGAAAGTCCAATCGAATTAGTTGCTGCTTT
AGATGAATCAGATAAAGCTGCCCAAATTAAAGAATTAGTAACTGAAATCGCTGAGCTTTCTGACAAAGTGACTGCCCGTT
TTGATGGCAACAATACACGCCGTCCAAGCTTTGGTGTGGCTAAAGCAGGTGAACAACCCCGTGTGTTCTTTGCAGGCTTA
CCAATGGGCCATGAGTTTACGTCTTTGATCTTGGCTCTGTTACAAGTGTCTGGCTATGCACCTAAAGTGTCGGATGAAGT
TTTAAATCAGATTAAAGGCTTAAACCTCAAAGCTAACTTTGATGTATTCGTATCATTAAGCTGTCATAACTGTCCGGATG
TGGTACAGGCGCTTAACCTGATTGCTATTTATAATCCGAACACCACTGCAACCATGATTGATGGTGCATTCTTCCAAGAC
GAAGTGGAACAACGCAAAATCATGGCTGTACCGATGGTATTCCAGGACAATGAACACATTGGTCAAGGTCGTATGACCCT
TGAAGAGATTGTGGCAAAACTTGATACCAATTCAGCTGAAAAAGACGCAGCAGCACTCAACGCCAAAGATGCGTTTGATG
TATTGGTGATTGGTGGTGGACCTGCGGGTGCAACAGCTGCAATCTATGCGGCACGTAAAGGCATTAACACAGGTATCGTG
GCTGAACGCTTTGGTGGTCAGGTCATGGACACCATGGACATTGAAAACTTCACTTCTGTGCAAAAGACTCAAGGTCCTAA
GTTTGCTGCCGAGATGGAAGCACATGTTCGTGAATACGATGTAGATATCATGAACCTGCAACGTGTCAGCAAAATCACTG
GTGCAGACCAAACAGCAAATGGCTTGGTTGAAGTTGAACTTGAAAACGGTGCAAAACTTGAATCGAAAACTGTGATCCTT
TCAACAGGCGCACGTTGGAGAGAAATGAATGTACCGGGTGAAGCTGAATACCGTACCCGTGGTGTAGCATACTGCCCACA
CTGTGATGGTCCATTGTTCAAAGGCAAACGTGTTGCCGTGATTGGTGGTGGTAACTCAGGTGTTGAAGCTGCAATTGACC
TTGCTGGGATTGTTGAGCATGTAACTCTGGTTGAGTTCGACACTAAACTTCGTGCTGACCAAGTCTTGCAAAACAAGTTA
AATAGCTTGCCAAACACCACTGTGATTATGAATGCATTAAGCACAGAAGTATTGGGTGACGGTTCACAAGTAACCGGTCT
TAAATATAAAGACCGTACAACTGATGAAGAGCATGTGGTAGAGCTTGCAGGGATCTTTGTACAGATTGGTTTACTTCCAA
ACACTGACTTCTTAAAAGACAGTGAGGTTGAGTTAACCAACCGTGGTGAGATCATTGTGAATGACCGCAACGAAACCAAT
GTAAAAGGTGTATTTGCTGCAGGTGACTGTACTACTGTACCGTACAAGCAGATCATTATTGCTACAGGTGAAGGCGCTAA
AGCATCACTCTCTGCGTTTGATTACATCATCCGTTCTGGGCAATAA

Upstream 100 bases:

>100_bases
AAACCGATCAAATACATAAAAACATACTGTTTTACCTATTCATTGTTTTAACGTACTATGCATCTATTGAAAGAATTTGG
ATGCATTGGAGTAAAAACAG

Downstream 100 bases:

>100_bases
AATCTAACCTAAAACAAAAGAGCCAAAGGATATCCTTTGGCTCTTTTTATTTTTTATGGTTTATACAAACCATGTTCAAC
TAAATGCTCTCGTAAAATAC

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MLDQNIKTQLKAYLERLESPIELVAALDESDKAAQIKELVTEIAELSDKVTARFDGNNTRRPSFGVAKAGEQPRVFFAGL
PMGHEFTSLILALLQVSGYAPKVSDEVLNQIKGLNLKANFDVFVSLSCHNCPDVVQALNLIAIYNPNTTATMIDGAFFQD
EVEQRKIMAVPMVFQDNEHIGQGRMTLEEIVAKLDTNSAEKDAAALNAKDAFDVLVIGGGPAGATAAIYAARKGINTGIV
AERFGGQVMDTMDIENFTSVQKTQGPKFAAEMEAHVREYDVDIMNLQRVSKITGADQTANGLVEVELENGAKLESKTVIL
STGARWREMNVPGEAEYRTRGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLVEFDTKLRADQVLQNKL
NSLPNTTVIMNALSTEVLGDGSQVTGLKYKDRTTDEEHVVELAGIFVQIGLLPNTDFLKDSEVELTNRGEIIVNDRNETN
VKGVFAAGDCTTVPYKQIIIATGEGAKASLSAFDYIIRSGQ

Sequences:

>Translated_521_residues
MLDQNIKTQLKAYLERLESPIELVAALDESDKAAQIKELVTEIAELSDKVTARFDGNNTRRPSFGVAKAGEQPRVFFAGL
PMGHEFTSLILALLQVSGYAPKVSDEVLNQIKGLNLKANFDVFVSLSCHNCPDVVQALNLIAIYNPNTTATMIDGAFFQD
EVEQRKIMAVPMVFQDNEHIGQGRMTLEEIVAKLDTNSAEKDAAALNAKDAFDVLVIGGGPAGATAAIYAARKGINTGIV
AERFGGQVMDTMDIENFTSVQKTQGPKFAAEMEAHVREYDVDIMNLQRVSKITGADQTANGLVEVELENGAKLESKTVIL
STGARWREMNVPGEAEYRTRGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLVEFDTKLRADQVLQNKL
NSLPNTTVIMNALSTEVLGDGSQVTGLKYKDRTTDEEHVVELAGIFVQIGLLPNTDFLKDSEVELTNRGEIIVNDRNETN
VKGVFAAGDCTTVPYKQIIIATGEGAKASLSAFDYIIRSGQ
>Mature_521_residues
MLDQNIKTQLKAYLERLESPIELVAALDESDKAAQIKELVTEIAELSDKVTARFDGNNTRRPSFGVAKAGEQPRVFFAGL
PMGHEFTSLILALLQVSGYAPKVSDEVLNQIKGLNLKANFDVFVSLSCHNCPDVVQALNLIAIYNPNTTATMIDGAFFQD
EVEQRKIMAVPMVFQDNEHIGQGRMTLEEIVAKLDTNSAEKDAAALNAKDAFDVLVIGGGPAGATAAIYAARKGINTGIV
AERFGGQVMDTMDIENFTSVQKTQGPKFAAEMEAHVREYDVDIMNLQRVSKITGADQTANGLVEVELENGAKLESKTVIL
STGARWREMNVPGEAEYRTRGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLVEFDTKLRADQVLQNKL
NSLPNTTVIMNALSTEVLGDGSQVTGLKYKDRTTDEEHVVELAGIFVQIGLLPNTDFLKDSEVELTNRGEIIVNDRNETN
VKGVFAAGDCTTVPYKQIIIATGEGAKASLSAFDYIIRSGQ

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=292, Percent_Identity=27.0547945205479, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI65787454, Length=298, Percent_Identity=27.1812080536913, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI226437568, Length=298, Percent_Identity=27.1812080536913, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI87081763, Length=520, Percent_Identity=61.3461538461539, Blast_Score=641, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=314, Percent_Identity=33.7579617834395, Blast_Score=156, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6321898, Length=340, Percent_Identity=32.0588235294118, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6320560, Length=313, Percent_Identity=32.2683706070287, Blast_Score=141, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 56374; Mature: 56374

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDQNIKTQLKAYLERLESPIELVAALDESDKAAQIKELVTEIAELSDKVTARFDGNNTR
CCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCC
RPSFGVAKAGEQPRVFFAGLPMGHEFTSLILALLQVSGYAPKVSDEVLNQIKGLNLKANF
CCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEECC
DVFVSLSCHNCPDVVQALNLIAIYNPNTTATMIDGAFFQDEVEQRKIMAVPMVFQDNEHI
EEEEEEECCCCHHHHHHCCEEEEECCCCCEEEECCHHHHHHHHCCEEEEEEEEEECCCCC
GQGRMTLEEIVAKLDTNSAEKDAAALNAKDAFDVLVIGGGPAGATAAIYAARKGINTGIV
CCCCHHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCHH
AERFGGQVMDTMDIENFTSVQKTQGPKFAAEMEAHVREYDVDIMNLQRVSKITGADQTAN
HHHHCCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
GLVEVELENGAKLESKTVILSTGARWREMNVPGEAEYRTRGVAYCPHCDGPLFKGKRVAV
CEEEEEECCCCCCCCCEEEEECCCCEEECCCCCCCCCCCCCEEECCCCCCCCCCCCEEEE
IGGGNSGVEAAIDLAGIVEHVTLVEFDTKLRADQVLQNKLNSLPNTTVIMNALSTEVLGD
EECCCCCHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCC
GSQVTGLKYKDRTTDEEHVVELAGIFVQIGLLPNTDFLKDSEVELTNRGEIIVNDRNETN
CCCEECEEECCCCCCHHHHHHHHHHHEEEECCCCCCCCCCCCEEEECCCEEEEECCCCCC
VKGVFAAGDCTTVPYKQIIIATGEGAKASLSAFDYIIRSGQ
CEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDQNIKTQLKAYLERLESPIELVAALDESDKAAQIKELVTEIAELSDKVTARFDGNNTR
CCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCC
RPSFGVAKAGEQPRVFFAGLPMGHEFTSLILALLQVSGYAPKVSDEVLNQIKGLNLKANF
CCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEECC
DVFVSLSCHNCPDVVQALNLIAIYNPNTTATMIDGAFFQDEVEQRKIMAVPMVFQDNEHI
EEEEEEECCCCHHHHHHCCEEEEECCCCCEEEECCHHHHHHHHCCEEEEEEEEEECCCCC
GQGRMTLEEIVAKLDTNSAEKDAAALNAKDAFDVLVIGGGPAGATAAIYAARKGINTGIV
CCCCHHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCHH
AERFGGQVMDTMDIENFTSVQKTQGPKFAAEMEAHVREYDVDIMNLQRVSKITGADQTAN
HHHHCCCEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
GLVEVELENGAKLESKTVILSTGARWREMNVPGEAEYRTRGVAYCPHCDGPLFKGKRVAV
CEEEEEECCCCCCCCCEEEEECCCCEEECCCCCCCCCCCCCEEECCCCCCCCCCCCEEEE
IGGGNSGVEAAIDLAGIVEHVTLVEFDTKLRADQVLQNKLNSLPNTTVIMNALSTEVLGD
EECCCCCHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCC
GSQVTGLKYKDRTTDEEHVVELAGIFVQIGLLPNTDFLKDSEVELTNRGEIIVNDRNETN
CCCEECEEECCCCCCHHHHHHHHHHHEEEECCCCCCCCCCCCEEEECCCEEEEECCCCCC
VKGVFAAGDCTTVPYKQIIIATGEGAKASLSAFDYIIRSGQ
CEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]