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The map label for this gene is nfdA [H]

Identifier: 213157005

GI number: 213157005

Start: 1771768

End: 1773642

Strand: Direct

Name: nfdA [H]

Synonym: AB57_1683

Alternate gene names: 213157005

Gene position: 1771768-1773642 (Clockwise)

Preceding gene: 213157004

Following gene: 213157006

Centisome position: 43.74

GC content: 43.89

Gene sequence:

>1875_bases
ATGACCGAAATAAACCTCATACTTAAAAACGGTAAAATTACAACACTAGATCCTCAGAACCCCGAAGTACAAGCAATAGC
GATTGCAGATGGTAAAGTGGTACGTACGGGTACAACTGATGAGGTTATGAAACTCGCAACTCCAACAAGTAAAGTTGTAG
ACTTAAATGGTCGTCGCGTTATACCGGGTCTAAATGACAGTCATTTGCATATTATTCGTGGTGGTCTAAATTACAATATG
GAACTGCGCTGGGAAGGTGTGCCATCCGTTGCCGATGCATTACGCTTACTCAAAGAACAAGCAGATAATACGCCAGCTCC
ACAATGGGTTCGTGTCGTAGGCGGTTGGACTGAATTCCAGTTTGCCGAAAAGCGTTTACCGACTTTAGAAGAAATTAATA
AAGCTGCGCCAGATACTCCTGTATTCGTTTTACATCTATATGCCAGTGCTTTGTTGAACCGAGCTGCACTTGATGTATTA
GGCTTTAACAAAGATACACCTGACCCACCTGGTGGCAAGATTGTACGAAATGAAAAAGGTGAGCCAACAGGTCTCTTGCT
TGCTACGCCATCGGCTATGATTTTGTATTCGACCTTAGGGAAAGCACCGAAATTGCCAGTTGAAGATCAAGTCAATTCAA
CTCGACATTTCATGCGTGAATTGAACCGATTAGGTATTACTTCAGCAATTGATGCAGGTGGTGGTGGTCAAAACTATCCT
GAAGATTATGATGTGATTAAGCAATTGCACGATCAAAATCAAATGACAGTACGAATTGCTTATAACCTATTTGCTCAAAA
GGCAGGCCAAGAGCTTGATGATTATCGACGCTGGACAGAAATGACATTTCCGGGTGAGGGTGATGAGTTATTTAGAATGA
ATGGAGCAGGTGAGAACCTGACTTGGTCAGCGGGGGACTTTGAAGACTTCTATGAGCCACGTCCCGATTTACCAGAAAAA
ATGGAAGGTGAACTCGAAGCCATCGTAGAGCACTTGGCTGAAAAGAAATGGCCATTCCGTATTCATGCAACCTATGATGA
AAGTATTAGTCGGTTACTCAATGTATTTGAGCGCGTCAATTCAAAACAGCCATTTGCGACACGATTTATTATTGACCATG
CCGAAACCGTATCTGAGCGTAATATCGAACGTATTGGTGCGTTGGGTGGTGGTATCGCGATTCAGCATCGTATGGCATAT
CAAGGTGAGATTTTCGTAAAACGCTATGGTGCCGAAGCTGCCCAAGCAACGCCACCCGTTAAGAAAATGTTAGAACTCGG
TGTTCCTGTGGGTGCGGGAACGGATGCGACTCGTGTGGCCTCTTATAACCCTTGGGTGTGTTTGTATTGGCTGACTACAG
GTAAAACAGTAGGTGGGTTACCTTTGTATGATGAAAAAAATCTATTAGACCGTCAAACAGCGCTTAAACTCTGGACTAAA
GGTTCGGCTTGGTTCTCAGGTGAAAAAGATGTCAAAGGTTCACTCACAGCAGGGGAACTTGCCGACTTAGTTGTGTTGTC
AGATGACTACTTCAAAGTTGAAGCAGAAGATATCCAGTGGATTGAGTCTGTACTGACTGTTTTAGGCGGCAAAGTGGTAT
ATGCAGGGGCAGAGTTTAAGCAAGACGATCCGCCGTTACCTCCAGCATCGCCAACATGGTCGCCAGTAAAACGTTTTGGT
GGTCAGTGGCGTTTATCTGAAAATCGTAATGCACCTTCCAATCAATCATTACAGTCACAAAGTGCATTGTGTGAATGTGC
TAGCAGTTGTGGAATACATGGACACAGCCACGCTTGGATGCTTGATGTTCCTGTAAATGATAAGGATAAAAAATCTTTCT
GGGGCGCACTTGGGTGCTCGTGTTTTGCATTTTAA

Upstream 100 bases:

>100_bases
CGGTCAGACTCCGTCGTGTAAAATCAAAAAGAATAATGAAATATATATATTGCTATACCTTTATTTTTTAGAACGATAAT
TCACAAGAATGGAGTTCAGA

Downstream 100 bases:

>100_bases
ATAAAACAGTATGGGAACCTGCCGCATTAAATAGTTTGTTTCCTTAAGGTATGTACGATGAAAATGTACTTGTTATCTTT
GGCTGTAGGGTTGCTTGTTG

Product: amidohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 624; Mature: 623

Protein sequence:

>624_residues
MTEINLILKNGKITTLDPQNPEVQAIAIADGKVVRTGTTDEVMKLATPTSKVVDLNGRRVIPGLNDSHLHIIRGGLNYNM
ELRWEGVPSVADALRLLKEQADNTPAPQWVRVVGGWTEFQFAEKRLPTLEEINKAAPDTPVFVLHLYASALLNRAALDVL
GFNKDTPDPPGGKIVRNEKGEPTGLLLATPSAMILYSTLGKAPKLPVEDQVNSTRHFMRELNRLGITSAIDAGGGGQNYP
EDYDVIKQLHDQNQMTVRIAYNLFAQKAGQELDDYRRWTEMTFPGEGDELFRMNGAGENLTWSAGDFEDFYEPRPDLPEK
MEGELEAIVEHLAEKKWPFRIHATYDESISRLLNVFERVNSKQPFATRFIIDHAETVSERNIERIGALGGGIAIQHRMAY
QGEIFVKRYGAEAAQATPPVKKMLELGVPVGAGTDATRVASYNPWVCLYWLTTGKTVGGLPLYDEKNLLDRQTALKLWTK
GSAWFSGEKDVKGSLTAGELADLVVLSDDYFKVEAEDIQWIESVLTVLGGKVVYAGAEFKQDDPPLPPASPTWSPVKRFG
GQWRLSENRNAPSNQSLQSQSALCECASSCGIHGHSHAWMLDVPVNDKDKKSFWGALGCSCFAF

Sequences:

>Translated_624_residues
MTEINLILKNGKITTLDPQNPEVQAIAIADGKVVRTGTTDEVMKLATPTSKVVDLNGRRVIPGLNDSHLHIIRGGLNYNM
ELRWEGVPSVADALRLLKEQADNTPAPQWVRVVGGWTEFQFAEKRLPTLEEINKAAPDTPVFVLHLYASALLNRAALDVL
GFNKDTPDPPGGKIVRNEKGEPTGLLLATPSAMILYSTLGKAPKLPVEDQVNSTRHFMRELNRLGITSAIDAGGGGQNYP
EDYDVIKQLHDQNQMTVRIAYNLFAQKAGQELDDYRRWTEMTFPGEGDELFRMNGAGENLTWSAGDFEDFYEPRPDLPEK
MEGELEAIVEHLAEKKWPFRIHATYDESISRLLNVFERVNSKQPFATRFIIDHAETVSERNIERIGALGGGIAIQHRMAY
QGEIFVKRYGAEAAQATPPVKKMLELGVPVGAGTDATRVASYNPWVCLYWLTTGKTVGGLPLYDEKNLLDRQTALKLWTK
GSAWFSGEKDVKGSLTAGELADLVVLSDDYFKVEAEDIQWIESVLTVLGGKVVYAGAEFKQDDPPLPPASPTWSPVKRFG
GQWRLSENRNAPSNQSLQSQSALCECASSCGIHGHSHAWMLDVPVNDKDKKSFWGALGCSCFAF
>Mature_623_residues
TEINLILKNGKITTLDPQNPEVQAIAIADGKVVRTGTTDEVMKLATPTSKVVDLNGRRVIPGLNDSHLHIIRGGLNYNME
LRWEGVPSVADALRLLKEQADNTPAPQWVRVVGGWTEFQFAEKRLPTLEEINKAAPDTPVFVLHLYASALLNRAALDVLG
FNKDTPDPPGGKIVRNEKGEPTGLLLATPSAMILYSTLGKAPKLPVEDQVNSTRHFMRELNRLGITSAIDAGGGGQNYPE
DYDVIKQLHDQNQMTVRIAYNLFAQKAGQELDDYRRWTEMTFPGEGDELFRMNGAGENLTWSAGDFEDFYEPRPDLPEKM
EGELEAIVEHLAEKKWPFRIHATYDESISRLLNVFERVNSKQPFATRFIIDHAETVSERNIERIGALGGGIAIQHRMAYQ
GEIFVKRYGAEAAQATPPVKKMLELGVPVGAGTDATRVASYNPWVCLYWLTTGKTVGGLPLYDEKNLLDRQTALKLWTKG
SAWFSGEKDVKGSLTAGELADLVVLSDDYFKVEAEDIQWIESVLTVLGGKVVYAGAEFKQDDPPLPPASPTWSPVKRFGG
QWRLSENRNAPSNQSLQSQSALCECASSCGIHGHSHAWMLDVPVNDKDKKSFWGALGCSCFAF

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 69067; Mature: 68936

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEINLILKNGKITTLDPQNPEVQAIAIADGKVVRTGTTDEVMKLATPTSKVVDLNGRRV
CCEEEEEEECCEEEEECCCCCCEEEEEEECCEEEECCCHHHHHHHCCCCCEEEECCCCEE
IPGLNDSHLHIIRGGLNYNMELRWEGVPSVADALRLLKEQADNTPAPQWVRVVGGWTEFQ
CCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHEECCCHHHH
FAEKRLPTLEEINKAAPDTPVFVLHLYASALLNRAALDVLGFNKDTPDPPGGKIVRNEKG
HHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCC
EPTGLLLATPSAMILYSTLGKAPKLPVEDQVNSTRHFMRELNRLGITSAIDAGGGGQNYP
CCCEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCC
EDYDVIKQLHDQNQMTVRIAYNLFAQKAGQELDDYRRWTEMTFPGEGDELFRMNGAGENL
HHHHHHHHHHCCCCEEEEEHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCC
TWSAGDFEDFYEPRPDLPEKMEGELEAIVEHLAEKKWPFRIHATYDESISRLLNVFERVN
EECCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHC
SKQPFATRFIIDHAETVSERNIERIGALGGGIAIQHRMAYQGEIFVKRYGAEAAQATPPV
CCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCHHCCCCHH
KKMLELGVPVGAGTDATRVASYNPWVCLYWLTTGKTVGGLPLYDEKNLLDRQTALKLWTK
HHHHHHCCCCCCCCCCCEEECCCCCEEEEEEECCCEECCCCCCCCCCHHHHHHHHHHEEC
GSAWFSGEKDVKGSLTAGELADLVVLSDDYFKVEAEDIQWIESVLTVLGGKVVYAGAEFK
CCCCCCCCCCCCCCCCHHHHEEEEEECCCCEEEEHHHHHHHHHHHHHHCCEEEEECCCCC
QDDPPLPPASPTWSPVKRFGGQWRLSENRNAPSNQSLQSQSALCECASSCGIHGHSHAWM
CCCCCCCCCCCCHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEE
LDVPVNDKDKKSFWGALGCSCFAF
EEECCCCCHHHHHHHHHCCCEECC
>Mature Secondary Structure 
TEINLILKNGKITTLDPQNPEVQAIAIADGKVVRTGTTDEVMKLATPTSKVVDLNGRRV
CEEEEEEECCEEEEECCCCCCEEEEEEECCEEEECCCHHHHHHHCCCCCEEEECCCCEE
IPGLNDSHLHIIRGGLNYNMELRWEGVPSVADALRLLKEQADNTPAPQWVRVVGGWTEFQ
CCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHEECCCHHHH
FAEKRLPTLEEINKAAPDTPVFVLHLYASALLNRAALDVLGFNKDTPDPPGGKIVRNEKG
HHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCC
EPTGLLLATPSAMILYSTLGKAPKLPVEDQVNSTRHFMRELNRLGITSAIDAGGGGQNYP
CCCEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCC
EDYDVIKQLHDQNQMTVRIAYNLFAQKAGQELDDYRRWTEMTFPGEGDELFRMNGAGENL
HHHHHHHHHHCCCCEEEEEHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCC
TWSAGDFEDFYEPRPDLPEKMEGELEAIVEHLAEKKWPFRIHATYDESISRLLNVFERVN
EECCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHC
SKQPFATRFIIDHAETVSERNIERIGALGGGIAIQHRMAYQGEIFVKRYGAEAAQATPPV
CCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCHHCCCCHH
KKMLELGVPVGAGTDATRVASYNPWVCLYWLTTGKTVGGLPLYDEKNLLDRQTALKLWTK
HHHHHHCCCCCCCCCCCEEECCCCCEEEEEEECCCEECCCCCCCCCCHHHHHHHHHHEEC
GSAWFSGEKDVKGSLTAGELADLVVLSDDYFKVEAEDIQWIESVLTVLGGKVVYAGAEFK
CCCCCCCCCCCCCCCCHHHHEEEEEECCCCEEEEHHHHHHHHHHHHHHCCEEEEECCCCC
QDDPPLPPASPTWSPVKRFGGQWRLSENRNAPSNQSLQSQSALCECASSCGIHGHSHAWM
CCCCCCCCCCCCHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEE
LDVPVNDKDKKSFWGALGCSCFAF
EEECCCCCHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA