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The map label for this gene is cca [H]

Identifier: 212224963

GI number: 212224963

Start: 1683769

End: 1685133

Strand: Reverse

Name: cca [H]

Synonym: TON_1812

Alternate gene names: 212224963

Gene position: 1685133-1683769 (Counterclockwise)

Preceding gene: 212224964

Following gene: 212224961

Centisome position: 91.21

GC content: 53.99

Gene sequence:

>1365_bases
ATGGTTCAAGAAGTTATTGGGGAAGTCCTCGTCAGGATCAGGCCGAACGAAGAGGAGAGGGCTTTTATTGAAGGCCTGAT
GCGAGAGATCGAGAACATAGCCCGTGGAAGGGCTGAAGAGCTTGGCCTTGACATTAAACCTTATTTTGTTGGTTCCCTCG
CCAAGGACACTTACCTAGCCGGCGACCACGACGTTGACCTCTTCATGGCCTTTCCCCTCGACACTCCCCTCGAAGAGCTC
CGCGGGAAGGGCCTCGAGCTTGGTAAGGCCATAGCGGAGAGGCTCGATAGCTACGAAGTTGCCTACGCGGAGCACCCCTA
CGTGAGAGCCCGCTATAAAGGGGTCAAGGTTGATATAGTCCCCTGCTACGACGTCGACAGCTGGAAGGACGTCAGAACGG
CAGTTGATCGCTCCATACTCCACACGAAGTGGGTGCTTGAAAACCTCCACGGCAGAAACGACGATGTCAGGCTTTTGAAG
CGCTTCCTCAAGGGAATAAACGCCTACGGCAGCGAGATTTACGTGAGGGGCTTTTCGGGCTACCTTGCCGAAATCCTGAT
TATCAAGTACGGCTCATTCCTGGATGTTCTGGAGAAGGCCGACTTCATGCTGAGGCAGAAGATAATAGACCCTGCCGGCT
GGCTCAAGAGTGAACCAGAAATAGCCATGAAGACCATTAAGAGGGAAGCTGAAGGGGACAAGCCGCTCATCGTCATAGAC
CCCGTTGACCCGAGGAGGAACGTCGCCGCAAATCTGGGCTGGGAGAAGTTCGGGAGGTTCTACTTCAAGGCCCATCAATT
TCTGGAAGAGCCCCTCGTAGAGTTCTTCTTCCCGACGGAGAGCACGGGAGAGAACTACCTCGGAGAGCTCAGAAGAAAGA
GAACACACCTCGTCACGCTCCTCTTCAAAAAGCCCCAGCTTGTTGATGACCTGATCCTCCCGCAGCTGGAGAGGAGTGCC
AGAGGCTTCGAGAAAGCGTTGAGAAGGGAGAACTTCAGGGTTCTCGGCTGGGACTACGGCTACGTTGGGGAGGAAGCGTT
CATCATGCTGGAGCTGGACAGAATAGTGCGCGAGAAGATAACCATAAAGCCCGGCCCGGAGTTCTTCACTGAGAGGGGCT
GGGACTTCTACCGGAAGAACGAGAGGGTCTGGCTGATTGGAAAGAGGCTGTACGCGGAGAAGAGGGTTAAGGAGAGCATC
GTCGACGTCATTGTAGAGCTCCTGGAGAAGAATCAAGTTGCCTTGGGCAAGCAGGTAAGAGAGTTCATCCACTCCGCTGA
TATTCTGGTGGATTACGTTCCAGGGGAGCTCAAGAGAGAGGCATACCTCTTCCTGAGCAGGAAAAAGTGGAATTTGAAGG
GCTAA

Upstream 100 bases:

>100_bases
AGTTTGAGGTCGACGCTATAGAACTGAAGAAAAGCACGCTCACCCCCAAGGGCCCGATTTACGAGACCGTGGCGAGGTTT
GAACTGGCGGAGTGATGGTA

Downstream 100 bases:

>100_bases
GGCGGAAGTTGTCGTTCATCGAACAGCTTTTCAATCTTCTCCAGTATTCGTTTCAACTTCAAGTACTCCTCTCCATTCAG
GGTTCCAAATATCAGCTCCA

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MVQEVIGEVLVRIRPNEEERAFIEGLMREIENIARGRAEELGLDIKPYFVGSLAKDTYLAGDHDVDLFMAFPLDTPLEEL
RGKGLELGKAIAERLDSYEVAYAEHPYVRARYKGVKVDIVPCYDVDSWKDVRTAVDRSILHTKWVLENLHGRNDDVRLLK
RFLKGINAYGSEIYVRGFSGYLAEILIIKYGSFLDVLEKADFMLRQKIIDPAGWLKSEPEIAMKTIKREAEGDKPLIVID
PVDPRRNVAANLGWEKFGRFYFKAHQFLEEPLVEFFFPTESTGENYLGELRRKRTHLVTLLFKKPQLVDDLILPQLERSA
RGFEKALRRENFRVLGWDYGYVGEEAFIMLELDRIVREKITIKPGPEFFTERGWDFYRKNERVWLIGKRLYAEKRVKESI
VDVIVELLEKNQVALGKQVREFIHSADILVDYVPGELKREAYLFLSRKKWNLKG

Sequences:

>Translated_454_residues
MVQEVIGEVLVRIRPNEEERAFIEGLMREIENIARGRAEELGLDIKPYFVGSLAKDTYLAGDHDVDLFMAFPLDTPLEEL
RGKGLELGKAIAERLDSYEVAYAEHPYVRARYKGVKVDIVPCYDVDSWKDVRTAVDRSILHTKWVLENLHGRNDDVRLLK
RFLKGINAYGSEIYVRGFSGYLAEILIIKYGSFLDVLEKADFMLRQKIIDPAGWLKSEPEIAMKTIKREAEGDKPLIVID
PVDPRRNVAANLGWEKFGRFYFKAHQFLEEPLVEFFFPTESTGENYLGELRRKRTHLVTLLFKKPQLVDDLILPQLERSA
RGFEKALRRENFRVLGWDYGYVGEEAFIMLELDRIVREKITIKPGPEFFTERGWDFYRKNERVWLIGKRLYAEKRVKESI
VDVIVELLEKNQVALGKQVREFIHSADILVDYVPGELKREAYLFLSRKKWNLKG
>Mature_454_residues
MVQEVIGEVLVRIRPNEEERAFIEGLMREIENIARGRAEELGLDIKPYFVGSLAKDTYLAGDHDVDLFMAFPLDTPLEEL
RGKGLELGKAIAERLDSYEVAYAEHPYVRARYKGVKVDIVPCYDVDSWKDVRTAVDRSILHTKWVLENLHGRNDDVRLLK
RFLKGINAYGSEIYVRGFSGYLAEILIIKYGSFLDVLEKADFMLRQKIIDPAGWLKSEPEIAMKTIKREAEGDKPLIVID
PVDPRRNVAANLGWEKFGRFYFKAHQFLEEPLVEFFFPTESTGENYLGELRRKRTHLVTLLFKKPQLVDDLILPQLERSA
RGFEKALRRENFRVLGWDYGYVGEEAFIMLELDRIVREKITIKPGPEFFTERGWDFYRKNERVWLIGKRLYAEKRVKESI
VDVIVELLEKNQVALGKQVREFIHSADILVDYVPGELKREAYLFLSRKKWNLKG

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG1746

COG function: function code J; tRNA nucleotidyltransferase (CCA-adding enzyme)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008229
- InterPro:   IPR002934
- InterPro:   IPR011068
- InterPro:   IPR015329 [H]

Pfam domain/function: PF01909 NTP_transf_2; PF09249 tRNA_NucTransf2 [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 52911; Mature: 52911

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQEVIGEVLVRIRPNEEERAFIEGLMREIENIARGRAEELGLDIKPYFVGSLAKDTYLA
CHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHCCHHCCCEEC
GDHDVDLFMAFPLDTPLEELRGKGLELGKAIAERLDSYEVAYAEHPYVRARYKGVKVDIV
CCCCCEEEEECCCCCCHHHHCCCCCHHHHHHHHHHCCCEEEECCCCEEEEEECCEEEEEE
PCYDVDSWKDVRTAVDRSILHTKWVLENLHGRNDDVRLLKRFLKGINAYGSEIYVRGFSG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECHHH
YLAEILIIKYGSFLDVLEKADFMLRQKIIDPAGWLKSEPEIAMKTIKREAEGDKPLIVID
HHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHCCCCCCEEEEC
PVDPRRNVAANLGWEKFGRFYFKAHQFLEEPLVEFFFPTESTGENYLGELRRKRTHLVTL
CCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LFKKPQLVDDLILPQLERSARGFEKALRRENFRVLGWDYGYVGEEAFIMLELDRIVREKI
EECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEHHHHHHHHC
TIKPGPEFFTERGWDFYRKNERVWLIGKRLYAEKRVKESIVDVIVELLEKNQVALGKQVR
CCCCCHHHHHHCCCHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EFIHSADILVDYVPGELKREAYLFLSRKKWNLKG
HHHHHHHHEEEECCCHHHHHHHEEEECCCCCCCC
>Mature Secondary Structure
MVQEVIGEVLVRIRPNEEERAFIEGLMREIENIARGRAEELGLDIKPYFVGSLAKDTYLA
CHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHCCHHCCCEEC
GDHDVDLFMAFPLDTPLEELRGKGLELGKAIAERLDSYEVAYAEHPYVRARYKGVKVDIV
CCCCCEEEEECCCCCCHHHHCCCCCHHHHHHHHHHCCCEEEECCCCEEEEEECCEEEEEE
PCYDVDSWKDVRTAVDRSILHTKWVLENLHGRNDDVRLLKRFLKGINAYGSEIYVRGFSG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECHHH
YLAEILIIKYGSFLDVLEKADFMLRQKIIDPAGWLKSEPEIAMKTIKREAEGDKPLIVID
HHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHCCCCCCEEEEC
PVDPRRNVAANLGWEKFGRFYFKAHQFLEEPLVEFFFPTESTGENYLGELRRKRTHLVTL
CCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
LFKKPQLVDDLILPQLERSARGFEKALRRENFRVLGWDYGYVGEEAFIMLELDRIVREKI
EECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEHHHHHHHHC
TIKPGPEFFTERGWDFYRKNERVWLIGKRLYAEKRVKESIVDVIVELLEKNQVALGKQVR
CCCCCHHHHHHCCCHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EFIHSADILVDYVPGELKREAYLFLSRKKWNLKG
HHHHHHHHEEEECCCHHHHHHHEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA