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The map label for this gene is malE [H]

Identifier: 212224946

GI number: 212224946

Start: 1664644

End: 1665963

Strand: Reverse

Name: malE [H]

Synonym: TON_1795

Alternate gene names: 212224946

Gene position: 1665963-1664644 (Counterclockwise)

Preceding gene: 212224947

Following gene: 212224945

Centisome position: 90.17

GC content: 55.83

Gene sequence:

>1320_bases
ATGAAGAAAGGACTGTTTACCCTACTTTTGATTGGAGTTTTGATTTTTAGCGTCGTGGCCAGCGGCTGTATAGGCGGCGG
TGAAACTACCAGCCCCACCACCACTCAGCCAACAACCACCCCGAGCGAGACCACAACGACTACCAGCCCAACAGAAACAA
CTACCACTCCAGAGATCGAGTGCGGCAGCGGAAAAATAGTCATATGGCACGCCATGCAACCGAACGAGCTCGAGGTCTTC
CAGAGCCTCGCAGAGGAGTACATGGCCATGTGCCCGAACGTTGAGATAGTCTTCGAGCAGAAGCCGGATCTTGAGAGTGC
CCTCAAAGCCGCAATTCCGACCGGCCAGGGTCCGGACCTCTTCATATGGGCCCACGACTGGATTGGAAAGTTCGCCGATG
CTGGCCTTCTCGAGCCGATTGACGACTACGTTACCGACGACATTCTGAACCAGTTCGCCCCGATGGCTCAGGATGCTATG
CAGTACAAGGGTCACTTCTACGCCATGCCCTTCGCTGCCGAGACCGTTGCAATCATCTACAACAAGGATATGGTAAGCGA
GCCGCCCAAGACCTTCGACGAGATGAAGGCGATAATGGAGCAGTACTACGACCCGGACAACGATAAGTACGGCATAGCCT
ACCCAGTTAACGCTTACTTCATCTCTGCCTGGGCTCAGGCCTTTGGAGGCTACTACTTCAACGACCAGACCGAGATGCCC
GGTCTCGACCAGCCTGAGACCATAGAGGGCTTCGAGTTCTTCTTCCAGAACGTCTGGCCATACATGGCCCCAACCGCTGA
CTACAACACCCAGCAGAGCATCTTCCTCGAGGGCCGTGCTCCGATGATGGTCAACGGTCCGTGGAGCATTGCCAGCGTCA
AGGACGCTGGAATCAACTTCGGCATCGTCCCGCTCCCGCCGATAATCAAGGACGGTAAGGAGTACTGGCCGAGACCCTAT
GGTGGAGTCAAGAACATCTACTTCGCCAAGGGCATAAATAACAAAGAGACCGCCTGGTACTTCGTCAAGTGGTTCACCAC
CAATCCAGAGGCCATAAAGGAGCTCTCCCTCCAGCTCGGATACATCCCGGTCCTTTCACCGGTTCTTGAGGACCCAGAAA
TCCAAGCTGACCCCGTTATCTACGGCTTCGGTCAGGCAGTCCAGCACGCATACCTCATGCCAAAGAGCCCGAAGATGGGC
GCCGTTTGGGGCGGTGTTGACGGAGCCATCAACGAGATACTCCAGGACCCGGCCACCGCTGACATACCCGCCATACTCCA
GAAGTACCAGCAGGAGATCCTGAACAACCTCGGGGGCTGA

Upstream 100 bases:

>100_bases
GGGTTGTACACACAATGCAAACTTGAACAGGGAAGCGCAAGGTATATATATCACTGACGATGATACTATAACACCCAAAG
CGAATACGGGTGATAAAGCT

Downstream 100 bases:

>100_bases
AGCCCTTTCCCTTTTCCTACCTTTTTGGAGGGATTGGGATGAAGAAGATCACCACAATTGCTCTGTTCCTCATTCTTCCA
GGGATGGCGGCGTTTCTGCT

Product: ABC-type maltodextrin transport system

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: MMBP; Maltodextrin-binding protein [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MKKGLFTLLLIGVLIFSVVASGCIGGGETTSPTTTQPTTTPSETTTTTSPTETTTTPEIECGSGKIVIWHAMQPNELEVF
QSLAEEYMAMCPNVEIVFEQKPDLESALKAAIPTGQGPDLFIWAHDWIGKFADAGLLEPIDDYVTDDILNQFAPMAQDAM
QYKGHFYAMPFAAETVAIIYNKDMVSEPPKTFDEMKAIMEQYYDPDNDKYGIAYPVNAYFISAWAQAFGGYYFNDQTEMP
GLDQPETIEGFEFFFQNVWPYMAPTADYNTQQSIFLEGRAPMMVNGPWSIASVKDAGINFGIVPLPPIIKDGKEYWPRPY
GGVKNIYFAKGINNKETAWYFVKWFTTNPEAIKELSLQLGYIPVLSPVLEDPEIQADPVIYGFGQAVQHAYLMPKSPKMG
AVWGGVDGAINEILQDPATADIPAILQKYQQEILNNLGG

Sequences:

>Translated_439_residues
MKKGLFTLLLIGVLIFSVVASGCIGGGETTSPTTTQPTTTPSETTTTTSPTETTTTPEIECGSGKIVIWHAMQPNELEVF
QSLAEEYMAMCPNVEIVFEQKPDLESALKAAIPTGQGPDLFIWAHDWIGKFADAGLLEPIDDYVTDDILNQFAPMAQDAM
QYKGHFYAMPFAAETVAIIYNKDMVSEPPKTFDEMKAIMEQYYDPDNDKYGIAYPVNAYFISAWAQAFGGYYFNDQTEMP
GLDQPETIEGFEFFFQNVWPYMAPTADYNTQQSIFLEGRAPMMVNGPWSIASVKDAGINFGIVPLPPIIKDGKEYWPRPY
GGVKNIYFAKGINNKETAWYFVKWFTTNPEAIKELSLQLGYIPVLSPVLEDPEIQADPVIYGFGQAVQHAYLMPKSPKMG
AVWGGVDGAINEILQDPATADIPAILQKYQQEILNNLGG
>Mature_439_residues
MKKGLFTLLLIGVLIFSVVASGCIGGGETTSPTTTQPTTTPSETTTTTSPTETTTTPEIECGSGKIVIWHAMQPNELEVF
QSLAEEYMAMCPNVEIVFEQKPDLESALKAAIPTGQGPDLFIWAHDWIGKFADAGLLEPIDDYVTDDILNQFAPMAQDAM
QYKGHFYAMPFAAETVAIIYNKDMVSEPPKTFDEMKAIMEQYYDPDNDKYGIAYPVNAYFISAWAQAFGGYYFNDQTEMP
GLDQPETIEGFEFFFQNVWPYMAPTADYNTQQSIFLEGRAPMMVNGPWSIASVKDAGINFGIVPLPPIIKDGKEYWPRPY
GGVKNIYFAKGINNKETAWYFVKWFTTNPEAIKELSLQLGYIPVLSPVLEDPEIQADPVIYGFGQAVQHAYLMPKSPKMG
AVWGGVDGAINEILQDPATADIPAILQKYQQEILNNLGG

Specific function: Involved in an abc transport system for maltotriose [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=363, Percent_Identity=28.9256198347107, Blast_Score=129, Evalue=4e-31,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059
- InterPro:   IPR006061 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 48654; Mature: 48654

Theoretical pI: Translated: 4.05; Mature: 4.05

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01037 SBP_BACTERIAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGLFTLLLIGVLIFSVVASGCIGGGETTSPTTTQPTTTPSETTTTTSPTETTTTPEIE
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
CGSGKIVIWHAMQPNELEVFQSLAEEYMAMCPNVEIVFEQKPDLESALKAAIPTGQGPDL
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEE
FIWAHDWIGKFADAGLLEPIDDYVTDDILNQFAPMAQDAMQYKGHFYAMPFAAETVAIIY
EEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEE
NKDMVSEPPKTFDEMKAIMEQYYDPDNDKYGIAYPVNAYFISAWAQAFGGYYFNDQTEMP
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHCCEEECCCCCCC
GLDQPETIEGFEFFFQNVWPYMAPTADYNTQQSIFLEGRAPMMVNGPWSIASVKDAGINF
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEEECCCCCEEEECCCCCCC
GIVPLPPIIKDGKEYWPRPYGGVKNIYFAKGINNKETAWYFVKWFTTNPEAIKELSLQLG
CCCCCCHHHHCCHHHCCCCCCCCCCEEEECCCCCCCCEEEEEEEEECCHHHHHHHHHHCC
YIPVLSPVLEDPEIQADPVIYGFGQAVQHAYLMPKSPKMGAVWGGVDGAINEILQDPATA
CHHHHHHHHCCCCCCCCCEEEECHHHHHHHHCCCCCCCCCCEECCCHHHHHHHHCCCCCC
DIPAILQKYQQEILNNLGG
CHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKGLFTLLLIGVLIFSVVASGCIGGGETTSPTTTQPTTTPSETTTTTSPTETTTTPEIE
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
CGSGKIVIWHAMQPNELEVFQSLAEEYMAMCPNVEIVFEQKPDLESALKAAIPTGQGPDL
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEE
FIWAHDWIGKFADAGLLEPIDDYVTDDILNQFAPMAQDAMQYKGHFYAMPFAAETVAIIY
EEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEE
NKDMVSEPPKTFDEMKAIMEQYYDPDNDKYGIAYPVNAYFISAWAQAFGGYYFNDQTEMP
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHCCEEECCCCCCC
GLDQPETIEGFEFFFQNVWPYMAPTADYNTQQSIFLEGRAPMMVNGPWSIASVKDAGINF
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEEECCCCCEEEECCCCCCC
GIVPLPPIIKDGKEYWPRPYGGVKNIYFAKGINNKETAWYFVKWFTTNPEAIKELSLQLG
CCCCCCHHHHCCHHHCCCCCCCCCCEEEECCCCCCCCEEEEEEEEECCHHHHHHHHHHCC
YIPVLSPVLEDPEIQADPVIYGFGQAVQHAYLMPKSPKMGAVWGGVDGAINEILQDPATA
CHHHHHHHHCCCCCCCCCEEEECHHHHHHHHCCCCCCCCCCEECCCHHHHHHHHCCCCCC
DIPAILQKYQQEILNNLGG
CHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]