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The map label for this gene is pitB [C]

Identifier: 212224703

GI number: 212224703

Start: 1424246

End: 1425466

Strand: Reverse

Name: pitB [C]

Synonym: TON_1552

Alternate gene names: 212224703

Gene position: 1425466-1424246 (Counterclockwise)

Preceding gene: 212224704

Following gene: 212224702

Centisome position: 77.15

GC content: 53.97

Gene sequence:

>1221_bases
GTGATAAGCGTGGATCCATGGCTGCTGATAACCATCGTTCTTGGATTTGCGATGGCATGGGCCATCGGTGCCAATGATGC
CGCAAATTCTATGAGCACTGCTGTCGGTGCGAAGGCGATAACTCCGAAGCAGGCCGTGATCATAGCGGGTATCCTCGAGT
TCACCGGAGCCTACTTCTTCGGCAAGAGCGTCACAGAGACGATAAGGAAGGGCATACTCTACCCCGACAAAATCACAGAT
CCAACGGTTCTTATATACGGCTCAATAGCGGCCCTGCTTGCTGCAACGATTTGGCTGGTAGCGGCTACCAAATTCGGACT
CCCGGTTTCAACGACGCACTCGATCATAGGTGGCATAGCGGGCTACGGGATAGCCTACGCCGGCCTGGGCATCGTCAACT
GGGACAAGATGGCGCAGGTCGTCCTTAGCTGGATACTGTCCCCGGTAATAGGTGCCCTAATGGCTTACTTCATCTTCAAG
GCCCTTACCAAGAGCATTTTCGAAAGCGGAAATCCTGTGAGAAGTGCGAGGATATGGTCTCCCTTCTGGATAGGCATGGC
CTTCGTGGTCATCGGGGCCATGTTCTACATCAAGGTTCTCCACGGGAGTGACCTCAAGACGGGGGTCATTTTCTATGGAA
TACCTCTTGGCATAATCGTTTTCATCCTCAGCTACTTCCTCATAAAGGTCCGCTTCCCCTCGAGCGACCCGTACATTGGC
GTTGAGAGCATATTCAAGAAGGTGCAGGTGGTCACCTCTGCCTACGTGGCTTTAGCTCATGGAGCCAATGACGTCGCCAA
CGCAATAGGCCCTGTTGCCGCCGTTTACGCCGTCGCAACGATGGGTCTGGCTGGAATGCAGGTGCCCGTTCCGAGGTGGA
TTCTCGTCCTCGGTGGAGTAGGCATAGCGGTTGGCGTCGCAACCTACGGCTACCGCGTCATGGAGACGGTCGGCAAGAAA
ATAACAGAGCTTACCAATACAAGGGGCTTCACCATAGACTTCTCCGCTGCAACGGTGGTTCTCGTGGCTTCCTGGCTCGG
AATGCCGATCTCAACGACCCACACGGTGGTCGGTGCCGTCATAGGTATAGGCTTGGCTAGGGGAGTAAAGGCAATAAACA
AAAACATCGTGAGGGATATAATAATCTCCTGGTTCGTGACGGTTCCGATAGCGGCCGTTGTGAGCGCCATTATATTCAAG
GTGCTTATGGTGGTGGGGTGA

Upstream 100 bases:

>100_bases
CTGATTTCTCTTTATTCTATTTATGTCTATATAGAACTTTGCGGTGCACTATATTCTACTGCGGCAAGATATTTATATCT
TCGGGGGAAGCGTTCTCTCG

Downstream 100 bases:

>100_bases
TATCATGCAGGTGTGGACCAAACTCTTCGCGAAGAGCCCGTTCAAGCCTCTAATTAAGCACGCTGATGTGGTTCTCCAGA
CGGTTGAGACCCTCGAGAAG

Product: sodium/phosphate symporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MISVDPWLLITIVLGFAMAWAIGANDAANSMSTAVGAKAITPKQAVIIAGILEFTGAYFFGKSVTETIRKGILYPDKITD
PTVLIYGSIAALLAATIWLVAATKFGLPVSTTHSIIGGIAGYGIAYAGLGIVNWDKMAQVVLSWILSPVIGALMAYFIFK
ALTKSIFESGNPVRSARIWSPFWIGMAFVVIGAMFYIKVLHGSDLKTGVIFYGIPLGIIVFILSYFLIKVRFPSSDPYIG
VESIFKKVQVVTSAYVALAHGANDVANAIGPVAAVYAVATMGLAGMQVPVPRWILVLGGVGIAVGVATYGYRVMETVGKK
ITELTNTRGFTIDFSAATVVLVASWLGMPISTTHTVVGAVIGIGLARGVKAINKNIVRDIIISWFVTVPIAAVVSAIIFK
VLMVVG

Sequences:

>Translated_406_residues
MISVDPWLLITIVLGFAMAWAIGANDAANSMSTAVGAKAITPKQAVIIAGILEFTGAYFFGKSVTETIRKGILYPDKITD
PTVLIYGSIAALLAATIWLVAATKFGLPVSTTHSIIGGIAGYGIAYAGLGIVNWDKMAQVVLSWILSPVIGALMAYFIFK
ALTKSIFESGNPVRSARIWSPFWIGMAFVVIGAMFYIKVLHGSDLKTGVIFYGIPLGIIVFILSYFLIKVRFPSSDPYIG
VESIFKKVQVVTSAYVALAHGANDVANAIGPVAAVYAVATMGLAGMQVPVPRWILVLGGVGIAVGVATYGYRVMETVGKK
ITELTNTRGFTIDFSAATVVLVASWLGMPISTTHTVVGAVIGIGLARGVKAINKNIVRDIIISWFVTVPIAAVVSAIIFK
VLMVVG
>Mature_406_residues
MISVDPWLLITIVLGFAMAWAIGANDAANSMSTAVGAKAITPKQAVIIAGILEFTGAYFFGKSVTETIRKGILYPDKITD
PTVLIYGSIAALLAATIWLVAATKFGLPVSTTHSIIGGIAGYGIAYAGLGIVNWDKMAQVVLSWILSPVIGALMAYFIFK
ALTKSIFESGNPVRSARIWSPFWIGMAFVVIGAMFYIKVLHGSDLKTGVIFYGIPLGIIVFILSYFLIKVRFPSSDPYIG
VESIFKKVQVVTSAYVALAHGANDVANAIGPVAAVYAVATMGLAGMQVPVPRWILVLGGVGIAVGVATYGYRVMETVGKK
ITELTNTRGFTIDFSAATVVLVASWLGMPISTTHTVVGAVIGIGLARGVKAINKNIVRDIIISWFVTVPIAAVVSAIIFK
VLMVVG

Specific function: Potential transporter for phosphate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=162, Percent_Identity=44.4444444444444, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI5803173, Length=163, Percent_Identity=42.3312883435583, Blast_Score=145, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI25146401, Length=454, Percent_Identity=30.1762114537445, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17539280, Length=287, Percent_Identity=31.7073170731707, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71981576, Length=157, Percent_Identity=40.1273885350318, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32566716, Length=236, Percent_Identity=33.4745762711864, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17536725, Length=165, Percent_Identity=31.5151515151515, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17557328, Length=155, Percent_Identity=28.3870967741935, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319773, Length=164, Percent_Identity=38.4146341463415, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21356511, Length=187, Percent_Identity=37.9679144385027, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 42913; Mature: 42913

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA