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The map label for this gene is pyrE [H]

Identifier: 212224664

GI number: 212224664

Start: 1388778

End: 1389335

Strand: Reverse

Name: pyrE [H]

Synonym: TON_1513

Alternate gene names: 212224664

Gene position: 1389335-1388778 (Counterclockwise)

Preceding gene: 212224666

Following gene: 212224662

Centisome position: 75.2

GC content: 51.43

Gene sequence:

>558_bases
ATGAAGGACGAGCTCATCGAGATGATCTTTCGGGAAGGGGCTATACTCTTCGGGCGCTTTGTTCTCACCTCCGGAAAGGA
GAGCGACTACTACATCAACGTCAAAAAGCTCATCACGAATCCCCGGGCGCTGAAGCTTATTGCAAAGCTGATGAAATCAA
GGGTCGAGGAACTTGGCATCGAGTTTGATAAAGTGGCCGGCCCTGAGCTTGGGGCGGTTCCAATAGCCACTGCATTGGCT
CTCGAAACGGATAAATCCCTCATCATAGTCAGGAAAAAAAAGAAGGAGTACGGAACAGGAAAGCAGATAGAAGGTGAAGT
GACGCCTGGCGACAGGATACTCCTGGTCGAGGACGTCACAACCACAGGAGGCAGCGTCCTCAGGGCAGCTGAGATTCTTG
AGAAAGAGGGCGCGGTGGTTGTTGCTATAAGCGTTGTTGTGGACAGGGAAGAGGGGGCCAAAGAAAAAATGACGGAGAAG
TACACTTTTCTTCCGCTCGTTACGGTCTCAGAGCTTTTTGCCCGCAGGGATTCTGCCCCCTCGCAAATGAGAGAATAA

Upstream 100 bases:

>100_bases
TCCTCAGCCTTTATCTCTTATTAACCTGACGCTAAGATTTTTAAGTTTCGTGTCTATTTCTCCCAGAATTTACGCGACTA
TTAACATTAAGGTGTCCAAA

Downstream 100 bases:

>100_bases
TGATTTGTAGAGCCTGTGGAGAGACCCTTCCTTTTTCTTTTCCCCGAATATCCATTCACCCATGATGTCGCCCGCCTCGA
CGATGGCTTTTGAGGCTTTT

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MKDELIEMIFREGAILFGRFVLTSGKESDYYINVKKLITNPRALKLIAKLMKSRVEELGIEFDKVAGPELGAVPIATALA
LETDKSLIIVRKKKKEYGTGKQIEGEVTPGDRILLVEDVTTTGGSVLRAAEILEKEGAVVVAISVVVDREEGAKEKMTEK
YTFLPLVTVSELFARRDSAPSQMRE

Sequences:

>Translated_185_residues
MKDELIEMIFREGAILFGRFVLTSGKESDYYINVKKLITNPRALKLIAKLMKSRVEELGIEFDKVAGPELGAVPIATALA
LETDKSLIIVRKKKKEYGTGKQIEGEVTPGDRILLVEDVTTTGGSVLRAAEILEKEGAVVVAISVVVDREEGAKEKMTEK
YTFLPLVTVSELFARRDSAPSQMRE
>Mature_185_residues
MKDELIEMIFREGAILFGRFVLTSGKESDYYINVKKLITNPRALKLIAKLMKSRVEELGIEFDKVAGPELGAVPIATALA
LETDKSLIIVRKKKKEYGTGKQIEGEVTPGDRILLVEDVTTTGGSVLRAAEILEKEGAVVVAISVVVDREEGAKEKMTEK
YTFLPLVTVSELFARRDSAPSQMRE

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=153, Percent_Identity=38.562091503268, Blast_Score=119, Evalue=1e-27,
Organism=Escherichia coli, GI1790073, Length=154, Percent_Identity=33.7662337662338, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17554964, Length=174, Percent_Identity=35.0574712643678, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17508631, Length=171, Percent_Identity=31.5789473684211, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323530, Length=190, Percent_Identity=25.2631578947368, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323927, Length=178, Percent_Identity=25.8426966292135, Blast_Score=65, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17933654, Length=145, Percent_Identity=33.1034482758621, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 20444; Mature: 20444

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDELIEMIFREGAILFGRFVLTSGKESDYYINVKKLITNPRALKLIAKLMKSRVEELGI
CHHHHHHHHHHCCCCEEEHEEEECCCCCCEEEEHHHHHCCCHHHHHHHHHHHHHHHHHCC
EFDKVAGPELGAVPIATALALETDKSLIIVRKKKKEYGTGKQIEGEVTPGDRILLVEDVT
CHHHCCCCCCCCHHHHHHEEEECCCEEEEEEECCCCCCCCCEEECEECCCCEEEEEEECC
TTGGSVLRAAEILEKEGAVVVAISVVVDREEGAKEKMTEKYTFLPLVTVSELFARRDSAP
CCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHEEEHHHHHHHHHHHCCCCH
SQMRE
HHHCC
>Mature Secondary Structure
MKDELIEMIFREGAILFGRFVLTSGKESDYYINVKKLITNPRALKLIAKLMKSRVEELGI
CHHHHHHHHHHCCCCEEEHEEEECCCCCCEEEEHHHHHCCCHHHHHHHHHHHHHHHHHCC
EFDKVAGPELGAVPIATALALETDKSLIIVRKKKKEYGTGKQIEGEVTPGDRILLVEDVT
CHHHCCCCCCCCHHHHHHEEEECCCEEEEEEECCCCCCCCCEEECEECCCCEEEEEEECC
TTGGSVLRAAEILEKEGAVVVAISVVVDREEGAKEKMTEKYTFLPLVTVSELFARRDSAP
CCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHEEEHHHHHHHHHHHCCCCH
SQMRE
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA